research paper guidelines biology

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Formatting guide

This guide describes how to prepare contributions for submission. We recommend you read this in full if you have not previously submitted a contribution to Nature . We also recommend that, before submission, you familiarize yourself with Nature ’s style and content by reading the journal, either in print or online, particularly if you have not submitted to the journal recently.

Formats for Nature contributions

Articles are the main format for original research contributions to Nature . In addition, Nature publishes other submitted material as detailed below.

Articles are original reports whose conclusions represent a substantial advance in understanding of an important problem and have immediate, far-reaching implications. In print, physical sciences papers do not normally exceed 6 pages on average, and biological, clinical and social-sciences papers do not normally exceed 8 pages on average. However, the final print length is at the editor’s discretion.

Articles start with a fully referenced summary paragraph, ideally of no more than 200 words, which is separate from the main text and avoids numbers, abbreviations, acronyms or measurements unless essential. It is aimed at readers outside the discipline. This summary paragraph should be structured as follows: 2-3 sentences of basic-level introduction to the field; a brief account of the background and rationale of the work; a statement of the main conclusions (introduced by the phrase 'Here we show' or its equivalent); and finally, 2-3 sentences putting the main findings into general context so it is clear how the results described in the paper have moved the field forwards. Please refer to our annotated example   to see how the summary paragraph should be constructed.

The typical length of a 6-page article with 4 modest display items (figures and tables) is 2500 words (summary paragraph plus body text). The typical length of an 8-page article with 5-6 modest display items is 4300 words. A ‘modest’ display item is one that, with its legend, occupies about a quarter of a page (equivalent to ~270 words). If a composite figure (with several panels) needs to occupy at least half a page in order for all the elements to be visible, the text length may need to be reduced accordingly to accommodate such figures. Keep in mind that essential but technical details can be moved into the Methods or Supplementary Information.

As a guideline, articles typically have no more than 50 references. (There is no such constraint on any additional references associated with Methods or Supplementary Information.)

Sections are separated with subheadings to aid navigation. Subheadings may be up to 40 characters (including spaces).

Word counts refer to the text of the paper. Title, author list, acknowledgements and references are not included in total word counts.

Matters Arising and Corrections

Matters Arising are exceptionally interesting or important comments and clarifications on original research papers or other peer-reviewed material published within the past 18 months in Nature . They are published online but not in print.

For further details of and instructions for how to submit such comments on peer-reviewed material published in Nature — or to notify editors of the potential need for a correction — please consult our Matters Arising page.

Other contributions to Nature

Please access the other submitted material pages for further details on any of the contribution types below:

News and Comment

Correspondence

Books & Arts

News & Views

Insights, Reviews and Perspectives

Technology Features

The editorial process

See this section for an explanation of Nature 's editorial criteria for publication, refereeing policy and how editors handle papers after submission. Submission to a Nature journal is taken by the journal to mean that all the listed authors have agreed to all of the contents. See authorship policy for more details.

Presubmission enquiries

If you wish to enquire whether your Article might be suitable for consideration by Nature , please use our online presubmission enquiry service . All presubmission enquiries must include a cover paragraph to the editor stating the interest to a broad scientific readership, a fully referenced summary paragraph, and a reference list.

Readability

Nature is an international journal covering all the sciences. Contributions should therefore be written clearly and simply so that they are accessible to readers in other disciplines and to readers for whom English is not their first language. Thus, technical jargon should be avoided as far as possible and clearly explained where its use is unavoidable. Abbreviations, particularly those that are not standard, should also be kept to a minimum. The background, rationale and main conclusions of the study should be clearly explained. Titles and abstracts in particular should be written in language that will be readily intelligible to any scientist. Essential but specialized terms should be explained concisely but not didactically.

For gene, protein and other specialized names authors can use their preferred terminology so long as it is in current use by the community, but they must give all known names for the entity at first use in the paper. Nature prefers authors to use internationally agreed nomenclature. Papers containing new or revised formal taxonomic nomenclature for animals, whether living or extinct, are accepted conditional on the provision of LSIDs (Life Science Identifiers) by means of registration of such nomenclature with ZooBank, the proposed online registration system for the International Code of Zoological Nomenclature (ICZN).

Even though no paper will be rejected because of poor language, non–native English speakers occasionally receive feedback from editors and reviewers regarding language and grammar usage in their manuscripts. You may wish to consider asking colleagues to read your manuscript and/or to use a professional editing service such as those provided by our affiliates Nature Research Editing Service or American Journal Experts . You can also get a fast, free grammar check of your manuscript that takes into account all aspects of readability in English. Please note that the use of a language editing service is not a requirement for publication in Nature .

Nature 's editors provide detailed advice about the expected print length when asking for the final version of the manuscript. Nature 's editors often suggest revised titles and rewrite the summary paragraphs of Articles so the conclusions are clear to a broad readership.

After acceptance, Nature 's subeditors (copyeditors) ensure that the text and figures are readable and clear to those outside the field, and edit papers into Nature 's house style. They pay particular attention to summary paragraphs, overall clarity, figures, figure legends and titles.

Proofs are sent before publication; authors are welcome to discuss proposed changes with Nature 's subeditors, but Nature reserves the right to make the final decision about matters of style and the size of figures.

A useful set of articles providing general advice about writing and submitting scientific papers can be found on the SciDev.Net website.

Format of Articles

Contributions should be double-spaced and written in English (spellings as in the Oxford English Dictionary ).

Contributions should be organized in the sequence: title, authors, affiliations (plus present addresses), bold first paragraph, main text, main references, tables, figure legends, methods (including separate data and code availability statements), methods references, acknowledgements, author contributions, competing interest declaration, additional information (containing supplementary information line (if any) and corresponding author line), extended data figure/table legends. In order to facilitate the review process, for initial submissions we encourage authors to present the manuscript text and figures together in a single file (Microsoft Word or PDF, up to 30 MB in size). The figures may be inserted within the text at the appropriate positions or grouped at the end, and each figure legend should be presented together with its figure. Also, please include line numbers within the text.

Titles do not exceed two lines in print. This equates to 75 characters (including spaces). Titles do not normally include numbers, acronyms, abbreviations or punctuation. They should include sufficient detail for indexing purposes but be general enough for readers outside the field to appreciate what the paper is about.

An uninterrupted page of text contains about 1250 words.

A typical 6-page Article contains about 2,500 words of text and, additionally, 4 modest display items (figures and/or tables) with brief legends, reference list and online-only methods section if applicable. A composite figure (with several panels) usually needs to take about half a page, equivalent to about 600 words, in order for all the elements to be visible (see section 5.9 for instructions on sizing figures).

A typical 8-page Article contains about 4300 words of text and, additionally, 5-6 modest display items (figures and/or tables) with brief legends, reference list and online-only methods section if applicable. A composite figure (with several panels) usually needs to take about half a page, equivalent to about 600 words, in order for all the elements to be visible (see section 5.9 for instructions on sizing figures).

Authors of contributions that significantly exceed the limits stated here (or as specified by the editor) will have to shorten their papers before acceptance, inevitably delaying publication.

Nature requires authors to specify the contribution made by their co-authors in the end notes of the paper (see section 5.5). If authors regard it as essential to indicate that two or more co-authors are equal in status, they may be identified by an asterisk symbol with the caption ‘These authors contributed equally to this work’ immediately under the address list. If more than three co-authors are equal in status, this should be indicated in the author contributions statement. Present addresses appear immediately below the author list (below the footnote rule at the bottom of the first page) and may be identified by a dagger symbol; all other essential author-related explanation is placed in the acknowledgements.

Our preferred format for text is Microsoft Word, with the style tags removed.

TeX/LaTeX: If you have prepared your paper using TeX/LaTeX, we will need to convert this to Word after acceptance, before your paper can be typeset. All textual material of the paper (including references, tables, figure captions, online methods, etc.) should be included as a single .tex file.

We prefer the use of a ‘standard’ font, preferably 12-point Times New Roman. For mathematical symbols, Greek letters and other special characters, use normal text or Symbol font. Word Equation Editor/MathType should be used only for formulae that cannot be produced using normal text or Symbol font.

The ‘Methods’ section is in the main text file, following the figure legends. This Methods section will appear in the PDF and in the full-text (HTML) version of the paper online, but will not appear in the printed issue. The Methods section should be written as concisely as possible but should contain all elements necessary to allow interpretation and replication of the results. As a guideline, the Methods section does not typically exceed 3,000 words. To increase reproducibility, authors are encouraged to deposit a detailed description of protocols used in their study in a protocol sharing platform of their choice. Springer Nature’s protocols.io is a free and open service designed to help researchers share experimental know-how. Protocols deposited by the authors in www.protocols.io will be linked to the online Methods section upon publication

Detailed descriptions of methods already published should be avoided; a reference number can be provided to save space, with any new addition or variation stated.

The Methods section should be subdivided by short bold headings referring to methods used and we encourage the inclusion of specific subsections for statistics, reagents and animal models. If further references are included in this section their numbering should continue from the end of the last reference number in the rest of the paper and they are listed after the Methods section.

Please provide separate Data Availability and Code Availability statements after the main text statements and before the Extended Data legends; detailed guidance can be found in our data availability and data citations policy . Certain data types must be deposited in an appropriate public structured data depository (details are available here ), and the accession number(s) provided in the manuscript. Full access is required at the time of publication. Should full access to data be required for peer review, authors must provide it.

The Methods section cannot contain figures or tables (essential display items should be included in the Extended Data or exceptionally in the Supplementary Information).

References are each numbered, ordered sequentially as they appear in the text, tables, boxes, figure legends, Methods, Extended Data tables and Extended Data figure legends.

When cited in the text, reference numbers are superscript, not in brackets unless they are likely to be confused with a superscript number.

Do not use linked fields (produced by EndNote and similar programs). Please use the one-click button provided by EndNote to remove EndNote codes before saving your file.

As a guideline, Articles allow up to 50 references in the main text if needed and within the average page budget. Only one publication can be listed for each number. Additional references for Methods or Supplementary Information are not included in this count.

Only articles that have been published or accepted by a named publication, or that have been uploaded to a recognized preprint server (for example, arXiv, bioRxiv), should be in the reference list; papers in preparation should be mentioned in the text with a list of authors (or initials if any of the authors are co-authors of the present contribution).

Published conference abstracts, numbered patents, preprints on recognized servers, papers in press, and research datasets that have been assigned a digital object identifier may be included in reference lists, but text, grant details and acknowledgements may not. (An exception is the highlighted references which we ask authors of Reviews, Perspectives and Insights articles to provide.)

All authors should be included in reference lists unless there are more than five, in which case only the first author should be given, followed by ‘et al.’.

Please follow the style below in the published edition of Nature in preparing reference lists.

Authors should be listed surname first, followed by a comma and initials of given names.

Titles of all cited articles are required. Titles of articles cited in reference lists should be in upright, not italic text; the first word of the title is capitalized, the title written exactly as it appears in the work cited, ending with a full stop. Book titles are italic with all main words capitalized. Journal titles are italic and abbreviated according to common usage. Volume numbers are bold. The publisher and city of publication are required for books cited. (Refer to published papers in Nature for details.)

Research datasets may be cited in the reference list if they have been assigned digital object identifiers (DOIs) and include authors, title, publisher (repository name), identifier (DOI expressed as a URL). Example: Hao, Z., AghaKouchak, A., Nakhjiri, N. & Farahmand, A. Global Integrated Drought Monitoring and Prediction System (GIDMaPS) data sets. figshare http://dx.doi.org/10.6084/m9.figshare.853801 (2014).

Recognized preprints may be cited in the reference list. Example: Babichev, S. A., Ries, J. & Lvovsky, A. I. Quantum scissors: teleportation of single-mode optical states by means of a nonlocal single photon. Preprint at http://arXiv.org/quant-ph/0208066 (2002).

References to web-only journals should give authors, article title and journal name as above, followed by URL in full - or DOI if known - and the year of publication in parentheses.

References to websites should give authors if known, title of cited page, URL in full, and year of posting in parentheses.

End notes are brief and follow the Methods (or Methods References, if any).

Acknowledgements should be brief, and should not include thanks to anonymous referees and editors, inessential words, or effusive comments. A person can be thanked for assistance, not “excellent” assistance, or for comments, not “insightful” comments, for example. Acknowledgements can contain grant and contribution numbers.

Author Contributions: Authors are required to include a statement to specify the contributions of each co-author. The statement can be up to several sentences long, describing the tasks of individual authors referred to by their initials. See the authorship policy page for further explanation and examples.

Competing interests  statement.

Additional Information: Authors should include a set of statements at the end of the paper, in the following order:

Papers containing Supplementary Information contain the statement: “Supplementary Information is available for this paper.”

A sentence reading "Correspondence and requests for materials should be addressed to XX.” Nature expects this identified author to respond to readers’ enquiries and requests for materials, and to coordinate the handling of any other matters arising from the published contribution, including corrections complaints. The author named as corresponding author is not necessarily the senior author, and publication of this author’s name does not imply seniority. Authors may include more than one e-mail address if essential, in which event Nature will communicate with the first-listed address for any post-publication matters, and expect that author to coordinate with the other co-authors.

Peer review information includes the names of reviewers who agree to be cited and is completed by Nature staff during proofing.

A sentence reading “Reprints and permissions information is available at www.nature.com/reprints.”

Life sciences and behavioural & social sciences reporting guidelines

To improve the transparency of reporting and the reproducibility of published results, authors of life sciences and behavioural & social sciences Articles must provide a completed Reporting Summary that will be made available to editors and reviewers during manuscript assessment. The Reporting Summary will be published with all accepted manuscripts.

Please note: because of the advanced features used in these forms, you must use Adobe Reader to open the documents and fill them out.

Guidance and resources related to the use and reporting of statistics are available here .

Tables should each be presented on a separate page, portrait (not landscape) orientation, and upright on the page, not sideways.

Tables have a short, one-line title in bold text. Tables should be as small as possible. Bear in mind the size of a Nature page as a limiting factor when compiling a table.

Symbols and abbreviations are defined immediately below the table, followed by essential descriptive material as briefly as possible, all in double-spaced text.

Standard table formats are available for submissions of cryo-EM , NMR and X-ray crystallography data . Authors providing these data must use these standard tables and include them as Extended Data.

Figure legends

For initial submissions, we encourage authors to present the manuscript text and figures together in a single Word doc or PDF file, and for each figure legend to be presented together with its figure. However, when preparing the final paper to be accepted, we require figure legends to be listed one after the other, as part of the text document, separate from the figure files, and after the main reference list.

Each figure legend should begin with a brief title for the whole figure and continue with a short description of each panel and the symbols used. If the paper contains a Methods section, legends should not contain any details of methods. Legends should be fewer than 300 words each.

All error bars and statistics must be defined in the figure legend, as discussed above.

Nature requires figures in electronic format. Please ensure that all digital images comply with the Nature journals’ policy on image integrity .

Figures should be as small and simple as is compatible with clarity. The goal is for figures to be comprehensible to readers in other or related disciplines, and to assist their understanding of the paper. Unnecessary figures and parts (panels) of figures should be avoided: data presented in small tables or histograms, for instance, can generally be stated briefly in the text instead. Avoid unnecessary complexity, colouring and excessive detail.

Figures should not contain more than one panel unless the parts are logically connected; each panel of a multipart figure should be sized so that the whole figure can be reduced by the same amount and reproduced on the printed page at the smallest size at which essential details are visible. For guidance, Nature ’s standard figure sizes are 90 mm (single column) and 180 mm (double column) and the full depth of the page is 170 mm.

Amino-acid sequences should be printed in Courier (or other monospaced) font using the one-letter code in lines of 50 or 100 characters.

Authors describing chemical structures should use the Nature Research Chemical Structures style guide .

Some brief guidance for figure preparation:

Lettering in figures (labelling of axes and so on) should be in lower-case type, with the first letter capitalized and no full stop.

Units should have a single space between the number and the unit, and follow SI nomenclature or the nomenclature common to a particular field. Thousands should be separated by commas (1,000). Unusual units or abbreviations are defined in the legend.

Scale bars should be used rather than magnification factors.

Layering type directly over shaded or textured areas and using reversed type (white lettering on a coloured background) should be avoided where possible.

Where possible, text, including keys to symbols, should be provided in the legend rather than on the figure itself.

Figure quality

At initial submission, figures should be at good enough quality to be assessed by referees, preferably incorporated into the manuscript text in a single Word doc or PDF, although figures can be supplied separately as JPEGs if authors are unable to include them with the text. Authors are advised to follow the initial and revised submissions guidelines with respect to sizing, resolution and labelling.

Please note that print-publication quality figures are large and it is not helpful to upload them at the submission stage. Authors will be asked for high-quality figures when they are asked to submit the final version of their article for publication.At that stage, please prepare figures according to these guidelines .

Third party rights

Nature discourages the use or adaptation of previously published display items (for example, figures, tables, images, videos or text boxes). However, we recognize that to illustrate some concepts the use of published data is required and the reuse of previously published display items may be necessary. Please note that in these instances we might not be able to obtain the necessary rights for some images to be reused (as is, or adapted versions) in our articles. In such cases, we will contact you to discuss the sourcing of alternative material.

Figure costs

In order to help cover some of the additional cost of four-colour reproduction, Nature Portfolio charges our authors a fee for the printing of their colour figures. Please contact our offices for exact pricing and details. Inability to pay this charge will not prevent publication of colour figures judged essential by the editors, but this must be agreed with the editor prior to acceptance.

Production-quality figures

When a manuscript is accepted in principle for publication, the editor will ask for high-resolution figures. Do not submit publication-quality figures until asked to do so by an editor. At that stage, please prepare figures according to these guidelines .

Extended Data

Extended Data figures and tables are online-only (appearing in the online PDF and full-text HTML version of the paper), peer-reviewed display items that provide essential background to the Article but are not included in the printed version of the paper due to space constraints or being of interest only to a few specialists. A maximum of ten Extended Data display items (figures and tables) is typically permitted. See Composition of a Nature research paper .

Extended Data tables should be formatted along similar lines to tables appearing in print (see section 5.7) but the main body (excluding title and legend, which should be included at the end of the Word file) should be submitted separately as an image rather than as an editable format in Word, as Extended Data tables are not edited by Nature’s subediting department. Small tables may also be included as sub-panels within Extended Data figures. See Extended Data Formatting Guide .

Extended Data figures should be prepared along slightly different guidelines compared to figures appearing in print, and may be multi-panelled as long as they fit to size rules (see Extended Data Formatting Guide ). Extended Data figures are not edited or styled by Nature’s art department; for this reason, authors are requested to follow Nature style as closely as possible when preparing these figures. The legends for Extended Data figures should be prepared as for print figures and should be listed one after the other at the end of the Word file.

If space allows, Nature encourages authors to include a simple schematic, as a panel in an Extended Data figure, that summarizes the main finding of the paper, where appropriate (for example, to assist understanding of complex detail in cell, structural and molecular biology disciplines).

If a manuscript has Extended Data figures or tables, authors are asked to refer to discrete items at an appropriate place in the main text (for example, Extended Data Fig. 1 and Extended Data Table 1).

If further references are included in the Extended Data tables and Extended Data figure legends, the numbering should continue from the end of the last reference number in the main paper (or from the last reference number in the additional Methods section if present) and the list should be added to the end of the list accompanying the additional Methods section, if present, or added below the Extended Data legends if no additional Methods section is present.

Supplementary Information

Supplementary Information (SI) is online-only, peer-reviewed material that is essential background to the Article (for example, large data sets, methods, calculations), but which is too large or impractical, or of interest only to a few specialists, to justify inclusion in the printed version of the paper. See the Supplementary Information page for further details.

Supplementary Information should not contain figures (any figures additional to those appearing in print should be formatted as Extended Data figures). Tables may be included in Supplementary Information, but only if they are unsuitable for formatting as Extended Data tables (for example, tables containing large data sets or raw data that are best suited to Excel files).

If a manuscript has accompanying SI, either at submission or in response to an editor’s letter that requests it, authors are asked to refer to discrete items of the SI (for example, videos, tables) at an appropriate point in the main manuscript.

Chemical structures and characterization of chemical materials

For guidelines describing Nature ’s standards for experimental methods and the characterization of new compounds, please see the information sheet on the characterization of chemical materials .

We aim to produce chemical structures in a consistent format throughout our articles. Please use the Nature Portfolio Chemical Structures Guide and ChemDraw template to ensure that you prepare your figures in a format that will require minimal changes by our art and production teams. Submit final files at 100% as .cdx files.

Registered Reports

Registered Reports are empirical articles testing confirmatory hypotheses in which the methods and proposed analyses are pre-registered and peer reviewed prior to research being conducted. For further details about Registered Reports and instructions for how to submit such articles to Nature please consult our Registered Reports page.

All contributions should be submitted online , unless otherwise instructed by the editors. Please be sure to read the information on what to include in your cover letter as well as several important content-related issues when putting a submission together.

Before submitting, all contributors must agree to all of Nature's publication policies .

Nature authors must make data and materials publicly available upon publication. This includes deposition of data into the relevant databases and arranging for them to be publicly released by the online publication date (not after). A description of our initiative to improve the transparency and the reproducibility of published results is available here . A full description of Nature’s publication policies is at the Nature Portfolio Authors and Referees website .

Other Nature Research journals

An account of the relationship between all the Nature journals is provided at the Nature family page . 

Quick links

• Explore articles by subject
• Guide to authors
• Editorial policies

This document originally came from the Journal of Mammalogy courtesy of Dr. Ronald Barry, a former editor of the journal.

Biology Research: Getting Started: Writing & Citing

• Grant Proposals
• Research Design/Methods
• Finding Articles
• Writing & Citing
• Research Tips
• Background Information

Writing/Publishing

Links to Writing and Style Guides

• APA Style and Grammar Guidelines This comprehensive site from the American Psychological Association offers advice on topics that include paper formatting, in-text citations, references, and bias-free language.
• Ecological Society of America Style format for the journals Ecology, Ecological Applications, Ecological Monographs

Other Resources

• Conducting and Writing a Literature Review How to find and put together background information on your topic
• Guidelines for Effective Professional and Academic Writing A concise guide from the University of Florida/Institute of Food and Agricultural Sciences.
• How to Write Scientific Reports A comprehensive guide from the University of North Carolina Chapel Hill's Writing Center
• Materials and Methods An example of how to write up what was done and how it was done in your research.
• Poster and Presentation Resources Advice on presenting academic research and an extensive list of poster resources
• Writing in the Disciplines: Biology Examples of citing your sources from the University of Richmond Writing Center, from Bates College Department of Biology.

Selected Literature Review Books

Health and Sciences Librarian

Literature Review Tutorial

• Literature Reviews: An Overview for Graduate Students What is a literature review? What purpose does it serve in research? What should you expect when writing one? This tutorial from North Carolina State University answers these questions.

Creating Your Bibliography

• RefWorks Bibliographic management tools, such as RefWorks, allow you to organize and manage citations for your bibliography, and work with Microsoft Word to allow insertion of citations as you write a paper.
• RefWorks Information See the RefWorks for Biology page for more information.
• << Previous: Finding Articles
• Next: RefWorks >>
• Last Updated: Feb 1, 2024 1:56 PM
• URL: https://libguides.jsu.edu/bioresearch

Want to create or adapt books like this? Learn more about how Pressbooks supports open publishing practices.

13.1 Formatting a Research Paper

Learning objectives.

• Identify the major components of a research paper written using American Psychological Association (APA) style.
• Apply general APA style and formatting conventions in a research paper.

In this chapter, you will learn how to use APA style , the documentation and formatting style followed by the American Psychological Association, as well as MLA style , from the Modern Language Association. There are a few major formatting styles used in academic texts, including AMA, Chicago, and Turabian:

• AMA (American Medical Association) for medicine, health, and biological sciences
• APA (American Psychological Association) for education, psychology, and the social sciences
• Chicago—a common style used in everyday publications like magazines, newspapers, and books
• MLA (Modern Language Association) for English, literature, arts, and humanities
• Turabian—another common style designed for its universal application across all subjects and disciplines

While all the formatting and citation styles have their own use and applications, in this chapter we focus our attention on the two styles you are most likely to use in your academic studies: APA and MLA.

If you find that the rules of proper source documentation are difficult to keep straight, you are not alone. Writing a good research paper is, in and of itself, a major intellectual challenge. Having to follow detailed citation and formatting guidelines as well may seem like just one more task to add to an already-too-long list of requirements.

Following these guidelines, however, serves several important purposes. First, it signals to your readers that your paper should be taken seriously as a student’s contribution to a given academic or professional field; it is the literary equivalent of wearing a tailored suit to a job interview. Second, it shows that you respect other people’s work enough to give them proper credit for it. Finally, it helps your reader find additional materials if he or she wishes to learn more about your topic.

Furthermore, producing a letter-perfect APA-style paper need not be burdensome. Yes, it requires careful attention to detail. However, you can simplify the process if you keep these broad guidelines in mind:

• Work ahead whenever you can. Chapter 11 “Writing from Research: What Will I Learn?” includes tips for keeping track of your sources early in the research process, which will save time later on.
• Get it right the first time. Apply APA guidelines as you write, so you will not have much to correct during the editing stage. Again, putting in a little extra time early on can save time later.
• Use the resources available to you. In addition to the guidelines provided in this chapter, you may wish to consult the APA website at http://www.apa.org or the Purdue University Online Writing lab at http://owl.english.purdue.edu , which regularly updates its online style guidelines.

General Formatting Guidelines

This chapter provides detailed guidelines for using the citation and formatting conventions developed by the American Psychological Association, or APA. Writers in disciplines as diverse as astrophysics, biology, psychology, and education follow APA style. The major components of a paper written in APA style are listed in the following box.

These are the major components of an APA-style paper:

Body, which includes the following:

• Headings and, if necessary, subheadings to organize the content
• In-text citations of research sources
• References page

All these components must be saved in one document, not as separate documents.

The title page of your paper includes the following information:

• Title of the paper
• Author’s name
• Name of the institution with which the author is affiliated
• Header at the top of the page with the paper title (in capital letters) and the page number (If the title is lengthy, you may use a shortened form of it in the header.)

List the first three elements in the order given in the previous list, centered about one third of the way down from the top of the page. Use the headers and footers tool of your word-processing program to add the header, with the title text at the left and the page number in the upper-right corner. Your title page should look like the following example.

The next page of your paper provides an abstract , or brief summary of your findings. An abstract does not need to be provided in every paper, but an abstract should be used in papers that include a hypothesis. A good abstract is concise—about one hundred fifty to two hundred fifty words—and is written in an objective, impersonal style. Your writing voice will not be as apparent here as in the body of your paper. When writing the abstract, take a just-the-facts approach, and summarize your research question and your findings in a few sentences.

In Chapter 12 “Writing a Research Paper” , you read a paper written by a student named Jorge, who researched the effectiveness of low-carbohydrate diets. Read Jorge’s abstract. Note how it sums up the major ideas in his paper without going into excessive detail.

Write an abstract summarizing your paper. Briefly introduce the topic, state your findings, and sum up what conclusions you can draw from your research. Use the word count feature of your word-processing program to make sure your abstract does not exceed one hundred fifty words.

Depending on your field of study, you may sometimes write research papers that present extensive primary research, such as your own experiment or survey. In your abstract, summarize your research question and your findings, and briefly indicate how your study relates to prior research in the field.

Margins, Pagination, and Headings

APA style requirements also address specific formatting concerns, such as margins, pagination, and heading styles, within the body of the paper. Review the following APA guidelines.

Use these general guidelines to format the paper:

• Set the top, bottom, and side margins of your paper at 1 inch.
• Use double-spaced text throughout your paper.
• Use a standard font, such as Times New Roman or Arial, in a legible size (10- to 12-point).
• Use continuous pagination throughout the paper, including the title page and the references section. Page numbers appear flush right within your header.
• Section headings and subsection headings within the body of your paper use different types of formatting depending on the level of information you are presenting. Additional details from Jorge’s paper are provided.

Begin formatting the final draft of your paper according to APA guidelines. You may work with an existing document or set up a new document if you choose. Include the following:

• Your title page
• The abstract you created in Note 13.8 “Exercise 1”
• Correct headers and page numbers for your title page and abstract

APA style uses section headings to organize information, making it easy for the reader to follow the writer’s train of thought and to know immediately what major topics are covered. Depending on the length and complexity of the paper, its major sections may also be divided into subsections, sub-subsections, and so on. These smaller sections, in turn, use different heading styles to indicate different levels of information. In essence, you are using headings to create a hierarchy of information.

The following heading styles used in APA formatting are listed in order of greatest to least importance:

• Section headings use centered, boldface type. Headings use title case, with important words in the heading capitalized.
• Subsection headings use left-aligned, boldface type. Headings use title case.
• The third level uses left-aligned, indented, boldface type. Headings use a capital letter only for the first word, and they end in a period.
• The fourth level follows the same style used for the previous level, but the headings are boldfaced and italicized.
• The fifth level follows the same style used for the previous level, but the headings are italicized and not boldfaced.

Visually, the hierarchy of information is organized as indicated in Table 13.1 “Section Headings” .

Table 13.1 Section Headings

A college research paper may not use all the heading levels shown in Table 13.1 “Section Headings” , but you are likely to encounter them in academic journal articles that use APA style. For a brief paper, you may find that level 1 headings suffice. Longer or more complex papers may need level 2 headings or other lower-level headings to organize information clearly. Use your outline to craft your major section headings and determine whether any subtopics are substantial enough to require additional levels of headings.

Working with the document you developed in Note 13.11 “Exercise 2” , begin setting up the heading structure of the final draft of your research paper according to APA guidelines. Include your title and at least two to three major section headings, and follow the formatting guidelines provided above. If your major sections should be broken into subsections, add those headings as well. Use your outline to help you.

Because Jorge used only level 1 headings, his Exercise 3 would look like the following:

Citation Guidelines

In-text citations.

Throughout the body of your paper, include a citation whenever you quote or paraphrase material from your research sources. As you learned in Chapter 11 “Writing from Research: What Will I Learn?” , the purpose of citations is twofold: to give credit to others for their ideas and to allow your reader to follow up and learn more about the topic if desired. Your in-text citations provide basic information about your source; each source you cite will have a longer entry in the references section that provides more detailed information.

In-text citations must provide the name of the author or authors and the year the source was published. (When a given source does not list an individual author, you may provide the source title or the name of the organization that published the material instead.) When directly quoting a source, it is also required that you include the page number where the quote appears in your citation.

This information may be included within the sentence or in a parenthetical reference at the end of the sentence, as in these examples.

Epstein (2010) points out that “junk food cannot be considered addictive in the same way that we think of psychoactive drugs as addictive” (p. 137).

Here, the writer names the source author when introducing the quote and provides the publication date in parentheses after the author’s name. The page number appears in parentheses after the closing quotation marks and before the period that ends the sentence.

Addiction researchers caution that “junk food cannot be considered addictive in the same way that we think of psychoactive drugs as addictive” (Epstein, 2010, p. 137).

Here, the writer provides a parenthetical citation at the end of the sentence that includes the author’s name, the year of publication, and the page number separated by commas. Again, the parenthetical citation is placed after the closing quotation marks and before the period at the end of the sentence.

As noted in the book Junk Food, Junk Science (Epstein, 2010, p. 137), “junk food cannot be considered addictive in the same way that we think of psychoactive drugs as addictive.”

Here, the writer chose to mention the source title in the sentence (an optional piece of information to include) and followed the title with a parenthetical citation. Note that the parenthetical citation is placed before the comma that signals the end of the introductory phrase.

David Epstein’s book Junk Food, Junk Science (2010) pointed out that “junk food cannot be considered addictive in the same way that we think of psychoactive drugs as addictive” (p. 137).

Another variation is to introduce the author and the source title in your sentence and include the publication date and page number in parentheses within the sentence or at the end of the sentence. As long as you have included the essential information, you can choose the option that works best for that particular sentence and source.

Citing a book with a single author is usually a straightforward task. Of course, your research may require that you cite many other types of sources, such as books or articles with more than one author or sources with no individual author listed. You may also need to cite sources available in both print and online and nonprint sources, such as websites and personal interviews. Chapter 13 “APA and MLA Documentation and Formatting” , Section 13.2 “Citing and Referencing Techniques” and Section 13.3 “Creating a References Section” provide extensive guidelines for citing a variety of source types.

Writing at Work

APA is just one of several different styles with its own guidelines for documentation, formatting, and language usage. Depending on your field of interest, you may be exposed to additional styles, such as the following:

• MLA style. Determined by the Modern Languages Association and used for papers in literature, languages, and other disciplines in the humanities.
• Chicago style. Outlined in the Chicago Manual of Style and sometimes used for papers in the humanities and the sciences; many professional organizations use this style for publications as well.
• Associated Press (AP) style. Used by professional journalists.

References List

The brief citations included in the body of your paper correspond to the more detailed citations provided at the end of the paper in the references section. In-text citations provide basic information—the author’s name, the publication date, and the page number if necessary—while the references section provides more extensive bibliographical information. Again, this information allows your reader to follow up on the sources you cited and do additional reading about the topic if desired.

The specific format of entries in the list of references varies slightly for different source types, but the entries generally include the following information:

• The name(s) of the author(s) or institution that wrote the source
• The year of publication and, where applicable, the exact date of publication
• The full title of the source
• For books, the city of publication
• For articles or essays, the name of the periodical or book in which the article or essay appears
• For magazine and journal articles, the volume number, issue number, and pages where the article appears
• For sources on the web, the URL where the source is located

The references page is double spaced and lists entries in alphabetical order by the author’s last name. If an entry continues for more than one line, the second line and each subsequent line are indented five spaces. Review the following example. ( Chapter 13 “APA and MLA Documentation and Formatting” , Section 13.3 “Creating a References Section” provides extensive guidelines for formatting reference entries for different types of sources.)

In APA style, book and article titles are formatted in sentence case, not title case. Sentence case means that only the first word is capitalized, along with any proper nouns.

Key Takeaways

• Following proper citation and formatting guidelines helps writers ensure that their work will be taken seriously, give proper credit to other authors for their work, and provide valuable information to readers.
• Working ahead and taking care to cite sources correctly the first time are ways writers can save time during the editing stage of writing a research paper.
• APA papers usually include an abstract that concisely summarizes the paper.
• APA papers use a specific headings structure to provide a clear hierarchy of information.
• In APA papers, in-text citations usually include the name(s) of the author(s) and the year of publication.
• In-text citations correspond to entries in the references section, which provide detailed bibliographical information about a source.

Writing for Success Copyright © 2015 by University of Minnesota is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

Browse Course Material

Course info, instructors.

• Dr. Agi Stachowiak
• Prof. Alan Jasanoff
• Prof. Jacquin Niles
• Dr. Atissa Banuazizi
• Dr. Neal Lerner
• Dr. Linda Sutliff

Departments

• Biological Engineering

As Taught In

• Biomaterials
• Cell and Tissue Engineering

Learning Resource Types

Laboratory fundamentals in biological engineering, guidelines for writing up your research, list of authors, introduction, materials and methods.

• Figures and tables
• Figure and table legends

Recommended Order of Assembly for a Scientific Paper

Structure and repetition, results vs. discussion, holistic view of data, precision and detail, instructions for writing specific sections of a scientific paper.

A formal research article or less formal lab reports are the principal ways scientific data is conveyed to the rest of the scientific community and preserved for future examination. Each scientific journal has its own idiosyncrasies regarding particulars of the research article’s format, but the most common elements of a scientific article, in order of presentation, are:

• Results, including figures and tables

The requirements for each section are outlined below. If you want more information, you can find parts of this text in an on-line collection of instructional materials used in the Purdue University Writing Lab . Other parts are inspired by Robert A. Day’s book, “How to Write and Publish a Scientific Paper” from Oryx Press. Still other content is based on the research and teaching experiences of 20.109 instructors Natalie Kuldell and Agi Stachowiak.

The title should be short (about 10 words), interesting, and it should describe what you found. Avoid very broad titles (e.g., ‘DNA recombination’), and try to specify your own unique result within the limited space, while still maintaining readability of your title.

Indicate that you are the primary author of the report by underlining your name. Others who substantially contributed to your work, such as your lab partner, should also be listed.

The abstract serves as a condensed version (not >250 words) of your report, from motivational background to key results (and how they were found) to implications for the future. By convention, it should be single-spaced and not include citations.

The importance of abstracts is increasing as more scientists are using computers to keep up with the literature. Since many search engines can only search for words in a paper’s title and abstract, these may be the only parts that many people read. The abstract may also be the way a journal’s editor decides whether to send your paper out for peer review or reject it as uninteresting and not generally relevant. Consequently, a well written abstract is extraordinarily important.

The introduction provides a framework for the story you are about to tell, and thus serves two main purposes. For one, you must provide sufficient background information for a reader to understand the forthcoming results. Just as importantly, you must motivate the audience to keep reading! How? Reveal the significance of the work through connections to both prior scientific accomplishments and future applications. You are welcome to use your own creativity and judgement as to what a good introduction should look like; however, you may find the suggested structure below useful.

• Paragraph 1: most general, “big picture” paragraph. Here you should introduce the reader to the broader context of your experiment and motivate why your research is important. Be sure to tell a coherent story, not a dense but unconnected list of facts.
• Paragraph 2: “zooming in” somewhat. Now that the reader has a frame for thinking about your research, you can present background information in more depth.
• Paragraph 3: most specific, a description of your particular investigation.

The methods section should allow an independent investigator to repeat any of your experiments. Use sub-section headings to allow readers to quickly identify experiments of interest to them (e.g., “Protein conjugation to hydrogels” and “Cell culture and fluorescent labeling”). When commercially available kits were used, it is sufficient to cite the name of the kit and say that it was used according to the manufacturer’s protocol. The key to a good methods section is developing your judgement for what information is essential and what is extraneous.

Note that the methods section should be written in the past tense, since your experiments are completed at the time you are writing your paper. This section should also be written in complete sentences and paragraphs, not in bullet point form.

You should cite the class wiki at the beginning of this section as follows: “Protocols were according to the 20.109 Spring 2010 lab wiki: URL accessed on January 1, 2020, unless otherwise noted.”

The purpose of the results section is to present your data in a relatively unbiased way, but with some guiding framework. Begin with a short description of the goal and strategy of your overall experiment, and then delve into specific sub-sections that describe each piece of the work.

Titled sub-sections help create a high-level narrative and thus improve readability of dense papers. Effective sub-sections are organized by functional content, not by what you did each day in lab. For example, two successive titles might be “Conjugating lymphoid tissue-associated adhesion proteins to hydrogels” and “T cell migration in protein-coated hydrogels.” Less helpful titles for the same content would be “Hydrogel-protein reaction” and “Microscopy and velocity analysis.”

To write the results section, use the figures and tables as a guide. One approach you might take is to start by outlining, in point form, what you found, going slowly through each part of the figures. Then take the points and group them into paragraphs, and finally order the points within each paragraph. Present the data as fully as possible, including stuff that does not quite make sense at first glance. Ultimately, each sub-section should begin with an overview sentence that introduces the present experiment and end with a sentence stating the primary conclusion reached from that experiment. (Sub-section headings and figure caption titles can also emphasize said conclusion.) The overview and/or concluding sentences should also provide a transition to the previous/next piece of data when possible. Within a sub-section, be sure to stick to one topic per paragraph; sub-sections will generally require a few paragraphs each.

Note that verbs in the results section are usually in the past tense. Only established scientific knowledge is written about in the present tense, “the world is round,” for example. You cannot presume that your own data are part of the body of established scientific knowledge, and so when you describe your own results, use the past tense, “a band of 1.3 kb was seen,” for example. There are, however, exceptions to this general rule. It is acceptable to say, “Table 3 shows the sizes of the DNA fragments in our preparation.” It is also acceptable to say, “In a 1991 paper, Ebright and coworkers used PCR to mutagenize DNA.”

The purpose of the discussion section is to interpret and contextualize your data. You should begin by reiterating the purpose of your research and your major findings. Then you might do any or all of the following: connect your findings to other research (published or that of your peers); describe any ambiguities and sources of error in the data, and suggest future experiments to resolve uncertainties; explain where you expect your work may lead, and suggest specific experiments for extending your findings; describe any conceptual or technical limitations of the research. Finally, you should explain the significance of your findings to basic science and to engineering applications. Like the previous sections, the discussion should have a clear organization and narrative flow, whether or not you use sub-sections.

References are generally used in the Introduction and Discussion sections to support any claims that are not common knowledge. Include only those references that pertain to the question at hand. Journals vary considerably in their preferred format for the reference list. For this class, you should list the references alphabetically by the first author’s last name. Include all the authors, the paper’s title, the name of the journal in which it was published, its year of publication, the volume number, and page numbers. Please carefully follow the punctuation and format requirements. A typical reference should look like

Pavletich NP, Pabo CO. Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A. Science 1991; 252:809-817

In the body of your report, this article would be cited as follows: “The Crystal Structure of the Zif268-DNA Complex has been Solved (Pavletich 1991).”

If two or more articles can be cited for this finding, then they are listed alphabetically, separated by a comma.

Figures and Tables

Some readers begin by scanning the figures first. The figures, with the legends, should provide a self-explanatory overview of your data. Decide what the data show, then create figures which highlight the most important points of your paper.

Tables are used to present repetitive data that is numerical. Graphs or illustrations, collectively called figures, are used to present numerical trends, raw data (like a picture of a gel), or a model that explains your work.

When you prepare your figures and tables, keep in mind that it is significantly more expensive for journals to publish figures and tables than text, so try to present the data in a way that is worthy of such added expense. The table below is an example of an ineffective table.

The information in Table 1 could be presented in one sentence, such as: “In ten experiments carried out at 24°C, ion flow was detected only in the presence of cortical cells.” This is a clearer and more concise way to present the information. In addition, all tables and figures must have numbers, titles and legends.

Figure and Table Legends

Legends to the figures and tables explain the elements that appear in the illustration. Conclusions about the data are NOT included in the legends. As you write your first draft, you might state in a short simple sentence what the point of the figure or table is. In later drafts, make sure each element of the figure or table is explained. Your figure legends should be written in the present tense since you are explaining elements that still exist at the time that you are writing the paper.

Your data (results and figures) is the heart of your paper. Begin by assembling and understanding it as clearly as possible. Then you can write the framing material – introduction and discussion, while compiling a few relevant references. (Actually, you will write a draft of the introduction about halfway through the module, once you have begun to develop some understanding of protein engineering. The final revision is best written once you have all your results.) You should generally write the abstract last, once you have a big picture understanding of every element of your investigation. As for the methods section, you might write it in short pieces day-by-day, while the procedures are fresh in your mind.

Additional Scientific Writing Tips

In my experience, concentrating on the following topics tends to repay student effort.

The readability of your paper can be substantially improved by its organization. Well chosen sub-section titles provide the reader with a distillation of the high-level points you want to make. Take the opportunity early and often to summarize major points - if you do this right, you will feel like you are repeating yourself too much! Each major section of the report should be more or less stand-alone, that is, understandable without having read the rest of your paper. Be sure and work from big to small to big (from broad points to details and back) throughout your paper. Finally, limit paragraphs to one topic apiece, splitting up longer linked ideas as needed.

One difficult task for budding scientific writers is determining what goes in a Results vs. a Discussion section. Even at the professional level, different scientific journals may have somewhat different expectations for how much interpretation is appropriate in the Results. A good rule of thumb in most contexts is to draw technical conclusions in the Results, but save scientific conclusions for the Discussion. An alternative framework that should yield similar writing choices is to focus on relatively certain conclusions in the Results, and save more speculative (but still supported!) conclusions for the Discussion.

Example of a technical conclusion: “Sequence alignment revealed that both mutants exhibited mutations in at least one of the expected sites and thus were consistent with the possible nucleotide changes that could occur with the library used.”

Example of a scientific conclusion: “A previously reported model proposes that the OmpR-P phosphatase reaction occurs by hydrolysis rather than direct phosphotransfer from H557 (Hsing 1997). Our mutant is consistent with a change to the structure of EnvZ that facilitates this hydrolysis reaction, thus increasing phosphatase activity while concurrently reducing kinase activity.”

What should you do when two independent pieces of data suggest two very different conclusions about your overall experiment? The one thing you should not do is state that both conclusions are correct, in different parts of the paper, and hope that the reader will ignore the discrepancy! As a scientist, you must take a holistic view in your analyses. Is there any way to reconcile the two results? Is one method more trustworthy than the other or is there otherwise an indication to favour one conclusion over the other? If you have no way of determining which result is correct with your present data, can you propose future experiments to conclusively decide?

Writing concisely takes more effort than writing inefficient prose. It is also results in eminently more readable text that expresses your ideas more powerfully.

Being concise is not an invitation to being vague. Be sure to support your claims with specific examples, and to follow through on your ideas with sufficient detail.

The following table describes the features of reports that are excellent, good, and that need substantial improvement, respectively from left to right. Your own reports will be evaluated with these features in mind.

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April 29, 2024

Assembling muscles for flight

The CELF family of RNA binding proteins are important regulators of RNA processing during myogenesis. Elena Nikonova, Maria Spletter and colleagues show that the Drosophila homolog Bruno1 promotes a transition to mature splice isoforms, which is necessary for myofibrillogenesis and balanced growth in sarcomere length and width in flight muscles.

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BAK and BAX execute apoptotic cell death by increasing the permeability of the mitochondria outer membrane, a process regulated by interactions with the mitochondrial porin VDAC2. Zheng Yuan, Richard Birkinshaw, Peter Czabotar, Grant Dewson and co-authors map the key residues involved in the BAK:VDAC2 interaction and show that this interaction can be targeted to either promote or inhibit apoptosis.

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Methods and Resources

Blik: a 3D visualization tool for cryo-ET data

A common challenge encountered by researchers working on cryo-ET data is the incompatibility between different software tools. Lorenzo Gaifas, Irina Gutsche and co-workers develop a napari-based 3D visualization and analysis tool, named blik, that is compatible with commonly used cryo-ET processing packages.

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Unique role for VPS4A in separating daughter cells

The human AAA-ATPase VPS4 helps to separate daughter cells at the end of cytokinesis, but it has two paralogs, VPS4A and VPS4B; how do their functions differ? Inbar Dvilansky, Yarin Altaras, Natalie Elia and colleagues show that VPS4A has unique regulatory functions in cytokinesis which VPS4B cannot rescue, explaining the neurodevelopmental defects seen in people with VPS4A mutations.

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Resisting the forces of gastrulation

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Making brain pericytes

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Prioritizing UndP during cell wall synthesis

How do bacteria distribute the use of the lipid carrier UndP to the various pathways that require it? Ian Roney and David Rudner show that the Bacillus subtilis transcription factor sigma-M and its membrane-anchored anti-sigma factor monitor UndP levels and prioritize its use for cell wall synthesis.

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Unsolved Mystery

How do endosymbionts work with so few genes?

This Unsolved Mystery article explores how genome reduction alters endosymbiont biology and highlights a ‘tipping point’ where the loss of the ability to build a cell envelope coincides with a marked erosion of translation-related genes.

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Symbiosis: In search of a deeper understanding

Thomas Richards and Nancy Moran discuss our new collection of articles exploring emerging themes in symbiosis research, as researchers exploit modern research tools and new models to unravel how symbiotic interactions function and evolve.

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Perspective

Fungal holobionts to inform synthetic endosymbioses

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Fitness trade-offs and the origins of endosymbiosis

Endosymbiosis is common and has played an important role in the evolution of complex life. Michael Brockhurst, Duncan Cameron and Andrew Beckerman explore the theory and experimental evidence for trade-offs in the early-stage evolution of endosymbiosis.

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Novel role of LLGL2 silencing in autophagy: reversing epithelial-mesenchymal transition in prostate cancer

Prostate cancer (PCa) is a major urological disease that is associated with significant morbidity and mortality in men. LLGL2 is the mammalian homolog of Lgl. It acts as a tumor suppressor in breast and hepati...

• View Full Text

Rapid development and mass production of SARS-CoV-2 neutralizing chicken egg yolk antibodies with protective efficacy in hamsters

Despite the record speed of developing vaccines and therapeutics against the SARS-CoV-2 virus, it is not a given that such success can be secured in future pandemics. In addition, COVID-19 vaccination and appl...

High-fat diet, microbiome-gut-brain axis signaling, and anxiety-like behavior in male rats

Obesity, associated with the intake of a high-fat diet (HFD), and anxiety are common among those living in modern urban societies. Recent studies suggest a role of microbiome-gut-brain axis signaling, includin...

General regulatory factors exert differential effects on nucleosome sliding activity of the ISW1a complex

Chromatin dynamics is deeply involved in processes that require access to DNA, such as transcriptional regulation. Among the factors involved in chromatin dynamics at gene regulatory regions are general regula...

Establishment of primary prostate epithelial and tumorigenic cell lines using a non-viral immortalization approach

Research on prostate cancer is mostly performed using cell lines derived from metastatic disease, not reflecting stages of tumor initiation or early progression. Establishment of cancer cell lines derived from...

The effect of diabetes mellitus on differentiation of mesenchymal stem cells into insulin-producing cells

Diabetes mellitus (DM) is a global epidemic with increasing incidences. DM is a metabolic disease associated with chronic hyperglycemia. Aside from conventional treatments, there is no clinically approved cure...

Control of astrocytic Ca 2+ signaling by nitric oxide-dependent S-nitrosylation of Ca 2+ homeostasis modulator 1 channels

Astrocytes Ca 2+ signaling play a central role in the modulation of neuronal function. Activation of metabotropic glutamate receptors (mGluR) by glutamate released during an increase in synaptic activity triggers ...

Increased levels and activation of the IL-17 receptor in microglia contribute to enhanced neuroinflammation in cerebellum of hyperammonemic rats

Patients with liver cirrhosis may show minimal hepatic encephalopathy (MHE) with mild cognitive impairment and motor incoordination. Rats with chronic hyperammonemia reproduce these alterations. Motor incoordi...

Identification and expression analysis of two steamer-like retrotransposons in the Chilean blue mussel ( Mytilus chilensis )

Disseminated neoplasia (DN) is a proliferative cell disorder of the circulatory system of bivalve mollusks. The disease is transmitted between individuals and can also be induced by external chemical agents su...

Noncoding RNAs in skeletal development and disorders

Protein-encoding genes only constitute less than 2% of total human genomic sequences, and 98% of genetic information was previously referred to as “junk DNA”. Meanwhile, non-coding RNAs (ncRNAs) consist of app...

Cx43 hemichannels and panx1 channels contribute to ethanol-induced astrocyte dysfunction and damage

Alcohol, a widely abused drug, significantly diminishes life quality, causing chronic diseases and psychiatric issues, with severe health, societal, and economic repercussions. Previously, we demonstrated that...

Galectins in epithelial-mesenchymal transition: roles and mechanisms contributing to tissue repair, fibrosis and cancer metastasis

Galectins are soluble glycan-binding proteins that interact with a wide range of glycoproteins and glycolipids and modulate a broad spectrum of physiological and pathological processes. The expression and subc...

Glutaminolysis regulates endometrial fibrosis in intrauterine adhesion via modulating mitochondrial function

Endometrial fibrosis, a significant characteristic of intrauterine adhesion (IUA), is caused by the excessive differentiation and activation of endometrial stromal cells (ESCs). Glutaminolysis is the metabolic...

The long-chain flavodoxin FldX1 improves the biodegradation of 4-hydroxyphenylacetate and 3-hydroxyphenylacetate and counteracts the oxidative stress associated to aromatic catabolism in Paraburkholderia xenovorans

Bacterial aromatic degradation may cause oxidative stress. The long-chain flavodoxin FldX1 of Paraburkholderia xenovorans LB400 counteracts reactive oxygen species (ROS). The aim of this study was to evaluate the...

MicroRNA-148b secreted by bovine oviductal extracellular vesicles enhance embryo quality through BPM/TGF-beta pathway

Extracellular vesicles (EVs) and their cargoes, including MicroRNAs (miRNAs) play a crucial role in cell-to-cell communication. We previously demonstrated the upregulation of bta-mir-148b in EVs from oviductal...

YME1L-mediated mitophagy protects renal tubular cells against cellular senescence under diabetic conditions

The senescence of renal tubular epithelial cells (RTECs) is crucial in the progression of diabetic kidney disease (DKD). Accumulating evidence suggests a close association between insufficient mitophagy and RT...

Effects of latroeggtoxin-VI on dopamine and α-synuclein in PC12 cells and the implications for Parkinson’s disease

Parkinson’s disease (PD) is characterized by death of dopaminergic neurons leading to dopamine deficiency, excessive α-synuclein facilitating Lewy body formation, etc. Latroeggtoxin-VI (LETX-VI), a proteinaceo...

Glial-restricted progenitor cells: a cure for diseased brain?

The central nervous system (CNS) is home to neuronal and glial cells. Traditionally, glia was disregarded as just the structural support across the brain and spinal cord, in striking contrast to neurons, alway...

Carbapenem-resistant hypervirulent ST23 Klebsiella pneumoniae with a highly transmissible dual-carbapenemase plasmid in Chile

The convergence of hypervirulence and carbapenem resistance in the bacterial pathogen Klebsiella pneumoniae represents a critical global health concern. Hypervirulent K. pneumoniae (hvKp) strains, frequently from...

Endometrial mesenchymal stromal/stem cells improve regeneration of injured endometrium in mice

The monthly regeneration of human endometrial tissue is maintained by the presence of human endometrial mesenchymal stromal/stem cells (eMSC), a cell population co-expressing the perivascular markers CD140b an...

Embryo development is impaired by sperm mitochondrial-derived ROS

Basal energetic metabolism in sperm, particularly oxidative phosphorylation, is known to condition not only their oocyte fertilising ability, but also the subsequent embryo development. While the molecular pat...

Fibroblasts inhibit osteogenesis by regulating nuclear-cytoplasmic shuttling of YAP in mesenchymal stem cells and secreting DKK1

Fibrous scars frequently form at the sites of bone nonunion when attempts to repair bone fractures have failed. However, the detailed mechanism by which fibroblasts, which are the main components of fibrous sc...

MSC-derived exosomes protect auditory hair cells from neomycin-induced damage via autophagy regulation

Sensorineural hearing loss (SNHL) poses a major threat to both physical and mental health; however, there is still a lack of effective drugs to treat the disease. Recently, novel biological therapies, such as ...

Alpha-synuclein dynamics bridge Type-I Interferon response and SARS-CoV-2 replication in peripheral cells

Increasing evidence suggests a double-faceted role of alpha-synuclein (α-syn) following infection by a variety of viruses, including SARS-CoV-2. Although α-syn accumulation is known to contribute to cell toxic...

Lactadherin immunoblockade in small extracellular vesicles inhibits sEV-mediated increase of pro-metastatic capacities

Tumor-derived small extracellular vesicles (sEVs) can promote tumorigenic and metastatic capacities in less aggressive recipient cells mainly through the biomolecules in their cargo. However, despite recent ad...

Integration of ATAC-seq and RNA-seq identifies MX1-mediated AP-1 transcriptional regulation as a therapeutic target for Down syndrome

Growing evidence has suggested that Type I Interferon (I-IFN) plays a potential role in the pathogenesis of Down Syndrome (DS). This work investigates the underlying function of MX1, an effector gene of I-IFN,...

The novel roles of YULINK in the migration, proliferation and glycolysis of pulmonary arterial smooth muscle cells: implications for pulmonary arterial hypertension

Abnormal remodeling of the pulmonary vasculature, characterized by the proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) along with dysregulated glycolysis, is a pathognomonic feat...

Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model

Impaired pattern separation occurs in the early stage of Alzheimer’s disease (AD), and hippocampal dentate gyrus (DG) neurogenesis participates in pattern separation. Here, we investigated whether spatial memo...

Role of SYVN1 in the control of airway remodeling in asthma protection by promoting SIRT2 ubiquitination and degradation

Asthma is a heterogenous disease that characterized by airway remodeling. SYVN1 (Synoviolin 1) acts as an E3 ligase to mediate the suppression of endoplasmic reticulum (ER) stress through ubiquitination and de...

Advances towards the use of gastrointestinal tumor patient-derived organoids as a therapeutic decision-making tool

In December 2022 the US Food and Drug Administration (FDA) removed the requirement that drugs in development must undergo animal testing before clinical evaluation, a declaration that now demands the establish...

Melatonin alleviates pyroptosis by regulating the SIRT3/FOXO3α/ROS axis and interacting with apoptosis in Atherosclerosis progression

Atherosclerosis (AS), a significant contributor to cardiovascular disease (CVD), is steadily rising with the aging of the global population. Pyroptosis and apoptosis, both caspase-mediated cell death mechanism...

Prenatal ethanol exposure and changes in fetal neuroendocrine metabolic programming

Prenatal ethanol exposure (PEE) (mainly through maternal alcohol consumption) has become widespread. However, studies suggest that it can cause intrauterine growth retardation (IUGR) and multi-organ developmen...

Autologous non-invasively derived stem cells mitochondria transfer shows therapeutic advantages in human embryo quality rescue

The decline in the quantity and quality of mitochondria are closely associated with infertility, particularly in advanced maternal age. Transferring autologous mitochondria into the oocytes of infertile female...

Development of synthetic modulator enabling long-term propagation and neurogenesis of human embryonic stem cell-derived neural progenitor cells

Neural progenitor cells (NPCs) are essential for in vitro drug screening and cell-based therapies for brain-related disorders, necessitating well-defined and reproducible culture systems. Current strategies em...

Heat-responsive microRNAs participate in regulating the pollen fertility stability of CMS-D2 restorer line under high-temperature stress

Anther development and pollen fertility of cytoplasmic male sterility (CMS) conditioned by Gossypium harknessii cytoplasm (CMS-D2) restorer lines are susceptible to continuous high-temperature (HT) stress in sum...

Chemogenetic inhibition of NTS astrocytes normalizes cardiac autonomic control and ameliorate hypertension during chronic intermittent hypoxia

Obstructive sleep apnea (OSA) is characterized by recurrent episodes of chronic intermittent hypoxia (CIH), which has been linked to the development of sympathoexcitation and hypertension. Furthermore, it has ...

SARS-CoV-2 spike protein S1 activates Cx43 hemichannels and disturbs intracellular Ca 2+ dynamics

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes the ongoing coronavirus disease 2019 (COVID-19). An aspect of high uncertainty is whether the SARS-CoV-2 per se or the systemic inflammation ...

The effect of zofenopril on the cardiovascular system of spontaneously hypertensive rats treated with the ACE2 inhibitor MLN-4760

Angiotensin converting enzyme 2 (ACE2) plays a crucial role in the infection cycle of SARS-CoV-2 responsible for formation of COVID-19 pandemic. In the cardiovascular system, the virus enters the cells by bind...

Two murine models of sepsis: immunopathological differences between the sexes—possible role of TGFβ1 in female resistance to endotoxemia

Endotoxic shock (ExSh) and cecal ligature and puncture (CLP) are models that induce sepsis. In this work, we investigated early immunologic and histopathologic changes induced by ExSh or CLP models in female a...

An intracellular, non-oxidative factor activates in vitro chromatin fragmentation in pig sperm

In vitro incubation of epididymal and vas deferens sperm with Mn 2+ induces Sperm Chromatin Fragmentation (SCF), a mechanism that causes double-stranded breaks in toroid-linker regions (TLRs). Whether this mechani...

Focal ischemic stroke modifies microglia-derived exosomal miRNAs: potential role of mir-212-5p in neuronal protection and functional recovery

Ischemic stroke is a severe type of stroke with high disability and mortality rates. In recent years, microglial exosome-derived miRNAs have been shown to be promising candidates for the treatment of ischemic ...

S -Nitrosylation in endothelial cells contributes to tumor cell adhesion and extravasation during breast cancer metastasis

Nitric oxide is produced by different nitric oxide synthases isoforms. NO activates two signaling pathways, one dependent on soluble guanylate cyclase and protein kinase G, and other where NO post-translationa...

Identifying pyroptosis- and inflammation-related genes in intracranial aneurysms based on bioinformatics analysis

Intracranial aneurysm (IA) is the most common cerebrovascular disease, and subarachnoid hemorrhage caused by its rupture can seriously impede nerve function. Pyroptosis is an inflammatory mode of cell death wh...

Drosophila Atlastin regulates synaptic vesicle mobilization independent of bone morphogenetic protein signaling

The endoplasmic reticulum (ER) contacts endosomes in all parts of a motor neuron, including the axon and presynaptic terminal, to move structural proteins, proteins that send signals, and lipids over long dist...

Mucin1 induced trophoblast dysfunction in gestational diabetes mellitus via Wnt/β-catenin pathway

To elucidate the role of Mucin1 (MUC1) in the trophoblast function (glucose uptake and apoptosis) of gestational diabetes mellitus (GDM) women through the Wnt/β-catenin pathway.

Human umbilical cord mesenchymal stem cells (hUC-MSCs) alleviate paclitaxel-induced spermatogenesis defects and maintain male fertility

Chemotherapeutic drugs can cause reproductive damage by affecting sperm quality and other aspects of male fertility. Stem cells are thought to alleviate the damage caused by chemotherapy drugs and to play role...

Exploring the Neandertal legacy of pancreatic ductal adenocarcinoma risk in Eurasians

The genomes of present-day non-Africans are composed of 1–3% of Neandertal-derived DNA as a consequence of admixture events between Neandertals and anatomically modern humans about 50–60 thousand years ago. Ne...

Identification and analysis of key hypoxia- and immune-related genes in hypertrophic cardiomyopathy

Hypertrophic cardiomyopathy (HCM), an autosomal dominant genetic disease, is the main cause of sudden death in adolescents and athletes globally. Hypoxia and immune factors have been revealed to be related to ...

How do prolonged anchorage-free lifetimes strengthen non-small-cell lung cancer cells to evade anoikis? – A link with altered cellular metabolomics

Malignant cells adopt anoikis resistance to survive anchorage-free stresses and initiate cancer metastasis. It is still unknown how varying periods of anchorage loss contribute to anoikis resistance, cell migr...

Single nucleotide polymorphisms associated with wine fermentation and adaptation to nitrogen limitation in wild and domesticated yeast strains

For more than 20 years, Saccharomyces cerevisiae has served as a model organism for genetic studies and molecular biology, as well as a platform for biotechnology (e.g., wine production). One of the important eco...

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Biological Research

ISSN: 0717-6287

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