two independent sample hypothesis test

Independent t-test for two samples

Introduction.

The independent t-test, also called the two sample t-test, independent-samples t-test or student's t-test, is an inferential statistical test that determines whether there is a statistically significant difference between the means in two unrelated groups.

Null and alternative hypotheses for the independent t-test

The null hypothesis for the independent t-test is that the population means from the two unrelated groups are equal:

H 0 : u 1 = u 2

In most cases, we are looking to see if we can show that we can reject the null hypothesis and accept the alternative hypothesis, which is that the population means are not equal:

H A : u 1 ≠ u 2

To do this, we need to set a significance level (also called alpha) that allows us to either reject or accept the alternative hypothesis. Most commonly, this value is set at 0.05.

What do you need to run an independent t-test?

In order to run an independent t-test, you need the following:

• One independent, categorical variable that has two levels/groups.
• One continuous dependent variable.

Unrelated groups

Unrelated groups, also called unpaired groups or independent groups, are groups in which the cases (e.g., participants) in each group are different. Often we are investigating differences in individuals, which means that when comparing two groups, an individual in one group cannot also be a member of the other group and vice versa. An example would be gender - an individual would have to be classified as either male or female – not both.

Assumption of normality of the dependent variable

The independent t-test requires that the dependent variable is approximately normally distributed within each group.

Note: Technically, it is the residuals that need to be normally distributed, but for an independent t-test, both will give you the same result.

You can test for this using a number of different tests, but the Shapiro-Wilks test of normality or a graphical method, such as a Q-Q Plot, are very common. You can run these tests using SPSS Statistics, the procedure for which can be found in our Testing for Normality guide. However, the t-test is described as a robust test with respect to the assumption of normality. This means that some deviation away from normality does not have a large influence on Type I error rates. The exception to this is if the ratio of the smallest to largest group size is greater than 1.5 (largest compared to smallest).

What to do when you violate the normality assumption

If you find that either one or both of your group's data is not approximately normally distributed and groups sizes differ greatly, you have two options: (1) transform your data so that the data becomes normally distributed (to do this in SPSS Statistics see our guide on Transforming Data ), or (2) run the Mann-Whitney U test which is a non-parametric test that does not require the assumption of normality (to run this test in SPSS Statistics see our guide on the Mann-Whitney U Test ).

Assumption of homogeneity of variance

The independent t-test assumes the variances of the two groups you are measuring are equal in the population. If your variances are unequal, this can affect the Type I error rate. The assumption of homogeneity of variance can be tested using Levene's Test of Equality of Variances, which is produced in SPSS Statistics when running the independent t-test procedure. If you have run Levene's Test of Equality of Variances in SPSS Statistics, you will get a result similar to that below:

This test for homogeneity of variance provides an F -statistic and a significance value ( p -value). We are primarily concerned with the significance value – if it is greater than 0.05 (i.e., p > .05), our group variances can be treated as equal. However, if p < 0.05, we have unequal variances and we have violated the assumption of homogeneity of variances.

Overcoming a violation of the assumption of homogeneity of variance

If the Levene's Test for Equality of Variances is statistically significant, which indicates that the group variances are unequal in the population, you can correct for this violation by not using the pooled estimate for the error term for the t -statistic, but instead using an adjustment to the degrees of freedom using the Welch-Satterthwaite method. In all reality, you will probably never have heard of these adjustments because SPSS Statistics hides this information and simply labels the two options as "Equal variances assumed" and "Equal variances not assumed" without explicitly stating the underlying tests used. However, you can see the evidence of these tests as below:

From the result of Levene's Test for Equality of Variances, we can reject the null hypothesis that there is no difference in the variances between the groups and accept the alternative hypothesis that there is a statistically significant difference in the variances between groups. The effect of not being able to assume equal variances is evident in the final column of the above figure where we see a reduction in the value of the t -statistic and a large reduction in the degrees of freedom (df). This has the effect of increasing the p -value above the critical significance level of 0.05. In this case, we therefore do not accept the alternative hypothesis and accept that there are no statistically significant differences between means. This would not have been our conclusion had we not tested for homogeneity of variances.

Reporting the result of an independent t-test

When reporting the result of an independent t-test, you need to include the t -statistic value, the degrees of freedom (df) and the significance value of the test ( p -value). The format of the test result is: t (df) = t -statistic, p = significance value. Therefore, for the example above, you could report the result as t (7.001) = 2.233, p = 0.061.

Fully reporting your results

In order to provide enough information for readers to fully understand the results when you have run an independent t-test, you should include the result of normality tests, Levene's Equality of Variances test, the two group means and standard deviations, the actual t-test result and the direction of the difference (if any). In addition, you might also wish to include the difference between the groups along with a 95% confidence interval. For example:

Inspection of Q-Q Plots revealed that cholesterol concentration was normally distributed for both groups and that there was homogeneity of variance as assessed by Levene's Test for Equality of Variances. Therefore, an independent t-test was run on the data with a 95% confidence interval (CI) for the mean difference. It was found that after the two interventions, cholesterol concentrations in the dietary group (6.15 ± 0.52 mmol/L) were significantly higher than the exercise group (5.80 ± 0.38 mmol/L) ( t (38) = 2.470, p = 0.018) with a difference of 0.35 (95% CI, 0.06 to 0.64) mmol/L.

To know how to run an independent t-test in SPSS Statistics, see our SPSS Statistics Independent-Samples T-Test guide. Alternatively, you can carry out an independent-samples t-test using Excel, R and RStudio .

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The Two-Sample t -Test

What is the two-sample t -test.

The two-sample t -test (also known as the independent samples t -test) is a method used to test whether the unknown population means of two groups are equal or not.

Is this the same as an A/B test?

Yes, a two-sample t -test is used to analyze the results from A/B tests.

When can I use the test?

You can use the test when your data values are independent, are randomly sampled from two normal populations and the two independent groups have equal variances.

What if I have more than two groups?

Use a multiple comparison method. Analysis of variance (ANOVA) is one such method. Other multiple comparison methods include the Tukey-Kramer test of all pairwise differences, analysis of means (ANOM) to compare group means to the overall mean or Dunnett’s test to compare each group mean to a control mean.

What if the variances for my two groups are not equal?

You can still use the two-sample t- test. You use a different estimate of the standard deviation. 

What if my data isn’t nearly normally distributed?

If your sample sizes are very small, you might not be able to test for normality. You might need to rely on your understanding of the data. When you cannot safely assume normality, you can perform a nonparametric test that doesn’t assume normality.

See how to perform a two-sample t -test using statistical software

• Download JMP to follow along using the sample data included with the software.
• To see more JMP tutorials, visit the JMP Learning Library .

Using the two-sample t -test

The sections below discuss what is needed to perform the test, checking our data, how to perform the test and statistical details.

What do we need?

For the two-sample t -test, we need two variables. One variable defines the two groups. The second variable is the measurement of interest.

We also have an idea, or hypothesis, that the means of the underlying populations for the two groups are different. Here are a couple of examples:

• We have students who speak English as their first language and students who do not. All students take a reading test. Our two groups are the native English speakers and the non-native speakers. Our measurements are the test scores. Our idea is that the mean test scores for the underlying populations of native and non-native English speakers are not the same. We want to know if the mean score for the population of native English speakers is different from the people who learned English as a second language.
• We measure the grams of protein in two different brands of energy bars. Our two groups are the two brands. Our measurement is the grams of protein for each energy bar. Our idea is that the mean grams of protein for the underlying populations for the two brands may be different. We want to know if we have evidence that the mean grams of protein for the two brands of energy bars is different or not.

Two-sample t -test assumptions

To conduct a valid test:

• Data values must be independent. Measurements for one observation do not affect measurements for any other observation.
• Data in each group must be obtained via a random sample from the population.
• Data in each group are normally distributed .
• Data values are continuous.
• The variances for the two independent groups are equal.

For very small groups of data, it can be hard to test these requirements. Below, we'll discuss how to check the requirements using software and what to do when a requirement isn’t met.

Two-sample t -test example

One way to measure a person’s fitness is to measure their body fat percentage. Average body fat percentages vary by age, but according to some guidelines, the normal range for men is 15-20% body fat, and the normal range for women is 20-25% body fat.

Our sample data is from a group of men and women who did workouts at a gym three times a week for a year. Then, their trainer measured the body fat. The table below shows the data.

Table 1: Body fat percentage data grouped by gender

You can clearly see some overlap in the body fat measurements for the men and women in our sample, but also some differences. Just by looking at the data, it's hard to draw any solid conclusions about whether the underlying populations of men and women at the gym have the same mean body fat. That is the value of statistical tests – they provide a common, statistically valid way to make decisions, so that everyone makes the same decision on the same set of data values.

Checking the data

Let’s start by answering: Is the two-sample t -test an appropriate method to evaluate the difference in body fat between men and women?

• The data values are independent. The body fat for any one person does not depend on the body fat for another person.
• We assume the people measured represent a simple random sample from the population of members of the gym.
• We assume the data are normally distributed, and we can check this assumption.
• The data values are body fat measurements. The measurements are continuous.
• We assume the variances for men and women are equal, and we can check this assumption.

Before jumping into analysis, we should always take a quick look at the data. The figure below shows histograms and summary statistics for the men and women.

The two histograms are on the same scale. From a quick look, we can see that there are no very unusual points, or outliers . The data look roughly bell-shaped, so our initial idea of a normal distribution seems reasonable.

Examining the summary statistics, we see that the standard deviations are similar. This supports the idea of equal variances. We can also check this using a test for variances.

Based on these observations, the two-sample t -test appears to be an appropriate method to test for a difference in means.

How to perform the two-sample t -test

For each group, we need the average, standard deviation and sample size. These are shown in the table below.

Table 2: Average, standard deviation and sample size statistics grouped by gender

Without doing any testing, we can see that the averages for men and women in our samples are not the same. But how different are they? Are the averages “close enough” for us to conclude that mean body fat is the same for the larger population of men and women at the gym? Or are the averages too different for us to make this conclusion?

We'll further explain the principles underlying the two sample t -test in the statistical details section below, but let's first proceed through the steps from beginning to end. We start by calculating our test statistic. This calculation begins with finding the difference between the two averages:

$ 22.29 - 14.95 = 7.34 $

This difference in our samples estimates the difference between the population means for the two groups.

Next, we calculate the pooled standard deviation. This builds a combined estimate of the overall standard deviation. The estimate adjusts for different group sizes. First, we calculate the pooled variance:

$ s_p^2 = \frac{((n_1 - 1)s_1^2) + ((n_2 - 1)s_2^2)} {n_1 + n_2 - 2} $

$ s_p^2 = \frac{((10 - 1)5.32^2) + ((13 - 1)6.84^2)}{(10 + 13 - 2)} $

$ = \frac{(9\times28.30) + (12\times46.82)}{21} $

$ = \frac{(254.7 + 561.85)}{21} $

$ =\frac{816.55}{21} = 38.88 $

Next, we take the square root of the pooled variance to get the pooled standard deviation. This is:

$ \sqrt{38.88} = 6.24 $

We now have all the pieces for our test statistic. We have the difference of the averages, the pooled standard deviation and the sample sizes.  We calculate our test statistic as follows:

$ t = \frac{\text{difference of group averages}}{\text{standard error of difference}} = \frac{7.34}{(6.24\times \sqrt{(1/10 + 1/13)})} = \frac{7.34}{2.62} = 2.80 $

To evaluate the difference between the means in order to make a decision about our gym programs, we compare the test statistic to a theoretical value from the t- distribution. This activity involves four steps:

• We decide on the risk we are willing to take for declaring a significant difference. For the body fat data, we decide that we are willing to take a 5% risk of saying that the unknown population means for men and women are not equal when they really are. In statistics-speak, the significance level, denoted by α, is set to 0.05. It is a good practice to make this decision before collecting the data and before calculating test statistics.
• We calculate a test statistic. Our test statistic is 2.80.
• We find the theoretical value from the t- distribution based on our null hypothesis which states that the means for men and women are equal. Most statistics books have look-up tables for the t- distribution. You can also find tables online. The most likely situation is that you will use software and will not use printed tables. To find this value, we need the significance level (α = 0.05) and the degrees of freedom . The degrees of freedom ( df ) are based on the sample sizes of the two groups. For the body fat data, this is: $ df = n_1 + n_2 - 2 = 10 + 13 - 2 = 21 $ The t value with α = 0.05 and 21 degrees of freedom is 2.080.
• We compare the value of our statistic (2.80) to the t value. Since 2.80 > 2.080, we reject the null hypothesis that the mean body fat for men and women are equal, and conclude that we have evidence body fat in the population is different between men and women.

Statistical details

Let’s look at the body fat data and the two-sample t -test using statistical terms.

Our null hypothesis is that the underlying population means are the same. The null hypothesis is written as:

$ H_o:  \mathrm{\mu_1} =\mathrm{\mu_2} $

The alternative hypothesis is that the means are not equal. This is written as:

$ H_o:  \mathrm{\mu_1} \neq \mathrm{\mu_2} $

We calculate the average for each group, and then calculate the difference between the two averages. This is written as:

$\overline{x_1} -  \overline{x_2} $

We calculate the pooled standard deviation. This assumes that the underlying population variances are equal. The pooled variance formula is written as:

The formula shows the sample size for the first group as n 1 and the second group as n 2 . The standard deviations for the two groups are s 1 and s 2 . This estimate allows the two groups to have different numbers of observations. The pooled standard deviation is the square root of the variance and is written as s p .

What if your sample sizes for the two groups are the same? In this situation, the pooled estimate of variance is simply the average of the variances for the two groups:

$ s_p^2 = \frac{(s_1^2 + s_2^2)}{2} $

The test statistic is calculated as:

$ t = \frac{(\overline{x_1} -\overline{x_2})}{s_p\sqrt{1/n_1 + 1/n_2}} $

The numerator of the test statistic is the difference between the two group averages. It estimates the difference between the two unknown population means. The denominator is an estimate of the standard error of the difference between the two unknown population means. 

Technical Detail: For a single mean, the standard error is $ s/\sqrt{n} $  . The formula above extends this idea to two groups that use a pooled estimate for s (standard deviation), and that can have different group sizes.

We then compare the test statistic to a t value with our chosen alpha value and the degrees of freedom for our data. Using the body fat data as an example, we set α = 0.05. The degrees of freedom ( df ) are based on the group sizes and are calculated as:

$ df = n_1 + n_2 - 2 = 10 + 13 - 2 = 21 $

The formula shows the sample size for the first group as n 1 and the second group as n 2 .  Statisticians write the t value with α = 0.05 and 21 degrees of freedom as:

$ t_{0.05,21} $

The t value with α = 0.05 and 21 degrees of freedom is 2.080. There are two possible results from our comparison:

• The test statistic is lower than the t value. You fail to reject the hypothesis of equal means. You conclude that the data support the assumption that the men and women have the same average body fat.
• The test statistic is higher than the t value. You reject the hypothesis of equal means. You do not conclude that men and women have the same average body fat.

t -Test with unequal variances

When the variances for the two groups are not equal, we cannot use the pooled estimate of standard deviation. Instead, we take the standard error for each group separately. The test statistic is:

$ t = \frac{ (\overline{x_1} -  \overline{x_2})}{\sqrt{s_1^2/n_1 + s_2^2/n_2}} $

The numerator of the test statistic is the same. It is the difference between the averages of the two groups. The denominator is an estimate of the overall standard error of the difference between means. It is based on the separate standard error for each group.

The degrees of freedom calculation for the t value is more complex with unequal variances than equal variances and is usually left up to statistical software packages. The key point to remember is that if you cannot use the pooled estimate of standard deviation, then you cannot use the simple formula for the degrees of freedom.

Testing for normality

The normality assumption is more important   when the two groups have small sample sizes than for larger sample sizes.

Normal distributions are symmetric, which means they are “even” on both sides of the center. Normal distributions do not have extreme values, or outliers. You can check these two features of a normal distribution with graphs. Earlier, we decided that the body fat data was “close enough” to normal to go ahead with the assumption of normality. The figure below shows a normal quantile plot for men and women, and supports our decision.

You can also perform a formal test for normality using software. The figure above shows results of testing for normality with JMP software. We test each group separately. Both the test for men and the test for women show that we cannot reject the hypothesis of a normal distribution. We can go ahead with the assumption that the body fat data for men and for women are normally distributed.

Testing for unequal variances

Testing for unequal variances is complex. We won’t show the calculations in detail, but will show the results from JMP software. The figure below shows results of a test for unequal variances for the body fat data.

Without diving into details of the different types of tests for unequal variances, we will use the F test. Before testing, we decide to accept a 10% risk of concluding the variances are equal when they are not. This means we have set α = 0.10.

Like most statistical software, JMP shows the p -value for a test. This is the likelihood of finding a more extreme value for the test statistic than the one observed. It’s difficult to calculate by hand. For the figure above, with the F test statistic of 1.654, the p- value is 0.4561. This is larger than our α value: 0.4561 > 0.10. We fail to reject the hypothesis of equal variances. In practical terms, we can go ahead with the two-sample t -test with the assumption of equal variances for the two groups.

Understanding p-values

Using a visual, you can check to see if your test statistic is a more extreme value in the distribution. The figure below shows a t- distribution with 21 degrees of freedom.

Since our test is two-sided and we have set α = .05, the figure shows that the value of 2.080 “cuts off” 2.5% of the data in each of the two tails. Only 5% of the data overall is further out in the tails than 2.080. Because our test statistic of 2.80 is beyond the cut-off point, we reject the null hypothesis of equal means.

Putting it all together with software

The figure below shows results for the two-sample t -test for the body fat data from JMP software.

The results for the two-sample t -test that assumes equal variances are the same as our calculations earlier. The test statistic is 2.79996. The software shows results for a two-sided test and for one-sided tests. The two-sided test is what we want (Prob > |t|). Our null hypothesis is that the mean body fat for men and women is equal. Our alternative hypothesis is that the mean body fat is not equal. The one-sided tests are for one-sided alternative hypotheses – for example, for a null hypothesis that mean body fat for men is less than that for women.

We can reject the hypothesis of equal mean body fat for the two groups and conclude that we have evidence body fat differs in the population between men and women. The software shows a p -value of 0.0107. We decided on a 5% risk of concluding the mean body fat for men and women are different, when they are not. It is important to make this decision before doing the statistical test.

The figure also shows the results for the t- test that does not assume equal variances. This test does not use the pooled estimate of the standard deviation. As was mentioned above, this test also has a complex formula for degrees of freedom. You can see that the degrees of freedom are 20.9888. The software shows a p- value of 0.0086. Again, with our decision of a 5% risk, we can reject the null hypothesis of equal mean body fat for men and women.

Other topics

If you have more than two independent groups, you cannot use the two-sample t- test. You should use a multiple comparison   method. ANOVA, or analysis of variance, is one such method. Other multiple comparison methods include the Tukey-Kramer test of all pairwise differences, analysis of means (ANOM) to compare group means to the overall mean or Dunnett’s test to compare each group mean to a control mean.

What if my data are not from normal distributions?

If your sample size is very small, it might be hard to test for normality. In this situation, you might need to use your understanding of the measurements. For example, for the body fat data, the trainer knows that the underlying distribution of body fat is normally distributed. Even for a very small sample, the trainer would likely go ahead with the t -test and assume normality.

What if you know the underlying measurements are not normally distributed? Or what if your sample size is large and the test for normality is rejected? In this situation, you can use nonparametric analyses. These types of analyses do not depend on an assumption that the data values are from a specific distribution. For the two-sample t ­-test, the Wilcoxon rank sum test is a nonparametric test that could be used.

Calcworkshop

Two Sample T Test Defined w/ 7 Step-by-Step Examples!

// Last Updated: October 9, 2020 - Watch Video //

Did you know that the two sample t test is used to calculate the difference between population means?

Jenn, Founder Calcworkshop ® , 15+ Years Experience (Licensed & Certified Teacher)

It’s true!

Now, there 3 ways to calculate the difference between means, as listed below:

• If the population standard deviation is known (z-test)
• Independent samples with an un-known standard deviation (two-sample-t-test)
• pooled variances
• un-pooled variances
• Matched Pair

Let’s find out more!

So how do we compare the mean of some quantitative variables for two different populations?

If our parameters of interest are the population means , then the best approach is to take random samples from both populations and compare their sample means as noted on the Engineering Statistics Handbook .

In other words , we analyze the difference between two sample means to understand the average difference between the two populations. And as always, the larger the sample size the more accurate our inferences will be.

Just like we saw with one-sample means , we will either employ a z-test or t-test depending on whether or not the population standard deviation is known or unknown .

However, there is a component we must consider, if we have independent random samples where the population standard deviation is unknown – do we pool our variances ?

When we found the difference of population proportions, we automatically pooled our variances. However, with the difference of population means, we will have to check. We do this by finding an F-statistic .

If this F-statistic is less than or equal to the critical number, then we will pool our variances. Otherwise, we will not pool.

Please note, that it is infrequent to have two independent samples with equal, or almost equal, variances — therefore, the formula for un-pooled variations is more readily accepted for most high school statistics courses.

But it is an important skill to learn and understand, so we will be working through several examples of when we need to pool variances and when we do not.

Worked Example

For example, imagine the college provost at one school said their students study more, on average than those at the neighboring school.

However, the provost at the nearby school believed the study time was the same and wants to clear up the controversy.

So, independent random samples were taken from both schools, with the results stated below. And at a 5% significance level, the following significance test is conducted.

Two Sample T Test Pooled Example

Notice that we pooled our variances because our F-statistic yielded a value less than our critical value. The interpretation of our results are as follows:

• Since the p-value is greater than our significance level, we fail to reject the null hypothesis.
• And conclude that the students at both schools, on average, study the same amount.

Matched Pairs Test

But what do we do if the populations we wish to compare are not different but the same?

Meaning, the difference between means is due to the population’s varying conditions and not due to the experimental units in the study.

When this happens, we have what is called a Matched Pairs T Test .

The great thing about a paired t test is that it becomes a one-sample t-test on the differences.

And then we will calculate the sample mean and sample standard deviation, sometimes referred to as standard error, using these difference values.

Matched Pairs T Test Formula

What is important to remember with any of these tests, whether it be a z-test or a two-sample t-test, our conclusions will be the same as a one-sample test.

For example, once we find out the test statistic, we then determine our p-value, and if our p-value is less than or equal to our significance level, we will reject our null hypothesis.

One Sample Flow Chart

Two Sample Flow Chart

As the flow chart demonstrates above, our first step is to decide what type of test we are conducting. Is the standard deviation known? Do we have a one sample test or a two sample test or is it matched-pair?

Then, once we have identified the test we are using, our procedure is as follows:

• Calculate the test statistic
• Determine our p-value
• If our p-value is less than or equal to our significance level, we will reject our null hypothesis.
• Otherwise we fail to reject the null hypothesis

Together, we will work through various examples of all different hypothesis tests for the difference in population means, so we become comfortable with each formula and know why and how to use them effectively.

Two Sample T Test – Lesson & Examples (Video)

1 hr 22 min

• Introduction to Video: Two Sample Hypothesis Test for Population Means
• 00:00:37 – How to write a two sample hypothesis test when population standard deviation is known? (Example#1)
• Exclusive Content for Members Only
• 00:16:35 – Construct a two sample hypothesis test when population standard deviation is known (Example #2)
• 00:26:01 – What is a Two-Sample t-test? Pooled variances or non-pooled variances?
• 00:28:31 – Use a two sample t-test with un-pooled variances (Example #3)
• 00:37:48 – Create a two sample t-test and confidence interval with pooled variances (Example #4)
• 00:51:23 – Construct a two-sample t-test (Example #5)
• 00:59:47 – Matched Pair one sample t-test (Example #6)
• 01:09:38 – Use a match paired hypothesis test and provide a confidence interval for difference of means (Example #7)
• Practice Problems with Step-by-Step Solutions
• Chapter Tests with Video Solutions

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Hypothesis Testing for Means & Proportions

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Z score Table

t score Table

Tests with Two Independent Samples, Continuous Outcome

There are many applications where it is of interest to compare two independent groups with respect to their mean scores on a continuous outcome. Here we compare means between groups, but rather than generating an estimate of the difference, we will test whether the observed difference (increase, decrease or difference) is statistically significant or not. Remember, that hypothesis testing gives an assessment of statistical significance, whereas estimation gives an estimate of effect and both are important.

Here we discuss the comparison of means when the two comparison groups are independent or physically separate. The two groups might be determined by a particular attribute (e.g., sex, diagnosis of cardiovascular disease) or might be set up by the investigator (e.g., participants assigned to receive an experimental treatment or placebo). The first step in the analysis involves computing descriptive statistics on each of the two samples. Specifically, we compute the sample size, mean and standard deviation in each sample and we denote these summary statistics as follows:

for sample 1:

for sample 2:

The designation of sample 1 and sample 2 is arbitrary. In a clinical trial setting the convention is to call the treatment group 1 and the control group 2. However, when comparing men and women, for example, either group can be 1 or 2.  

In the two independent samples application with a continuous outcome, the parameter of interest in the test of hypothesis is the difference in population means, μ 1 -μ 2 . The null hypothesis is always that there is no difference between groups with respect to means, i.e.,

The null hypothesis can also be written as follows: H 0 : μ 1 = μ 2 . In the research hypothesis, an investigator can hypothesize that the first mean is larger than the second (H 1 : μ 1 > μ 2 ), that the first mean is smaller than the second (H 1 : μ 1 < μ 2 ), or that the means are different (H 1 : μ 1 ≠ μ 2 ). The three different alternatives represent upper-, lower-, and two-tailed tests, respectively. The following test statistics are used to test these hypotheses.

Test Statistics for Testing H 0 : μ 1 = μ 2

• if n 1 > 30 and n 2 > 30
• if n 1 < 30 or n 2 < 30

NOTE: The formulas above assume equal variability in the two populations (i.e., the population variances are equal, or s 1 2 = s 2 2 ). This means that the outcome is equally variable in each of the comparison populations. For analysis, we have samples from each of the comparison populations. If the sample variances are similar, then the assumption about variability in the populations is probably reasonable. As a guideline, if the ratio of the sample variances, s 1 2 /s 2 2 is between 0.5 and 2 (i.e., if one variance is no more than double the other), then the formulas above are appropriate. If the ratio of the sample variances is greater than 2 or less than 0.5 then alternative formulas must be used to account for the heterogeneity in variances.    

The test statistics include Sp, which is the pooled estimate of the common standard deviation (again assuming that the variances in the populations are similar) computed as the weighted average of the standard deviations in the samples as follows:

Because we are assuming equal variances between groups, we pool the information on variability (sample variances) to generate an estimate of the variability in the population. Note: Because Sp is a weighted average of the standard deviations in the sample, Sp will always be in between s 1 and s 2 .)

Data measured on n=3,539 participants who attended the seventh examination of the Offspring in the Framingham Heart Study are shown below.  

Suppose we now wish to assess whether there is a statistically significant difference in mean systolic blood pressures between men and women using a 5% level of significance.  

• Step 1. Set up hypotheses and determine level of significance

H 0 : μ 1 = μ 2

H 1 : μ 1 ≠ μ 2                       α=0.05

• Step 2. Select the appropriate test statistic.  

Because both samples are large ( > 30), we can use the Z test statistic as opposed to t. Note that statistical computing packages use t throughout. Before implementing the formula, we first check whether the assumption of equality of population variances is reasonable. The guideline suggests investigating the ratio of the sample variances, s 1 2 /s 2 2 . Suppose we call the men group 1 and the women group 2. Again, this is arbitrary; it only needs to be noted when interpreting the results. The ratio of the sample variances is 17.5 2 /20.1 2 = 0.76, which falls between 0.5 and 2 suggesting that the assumption of equality of population variances is reasonable. The appropriate test statistic is

• Step 3. Set up decision rule.  

This is a two-tailed test, using a Z statistic and a 5% level of significance. Reject H 0 if Z < -1.960 or is Z > 1.960.

• Step 4. Compute the test statistic.  

We now substitute the sample data into the formula for the test statistic identified in Step 2. Before substituting, we will first compute Sp, the pooled estimate of the common standard deviation.

Notice that the pooled estimate of the common standard deviation, Sp, falls in between the standard deviations in the comparison groups (i.e., 17.5 and 20.1). Sp is slightly closer in value to the standard deviation in the women (20.1) as there were slightly more women in the sample.   Recall, Sp is a weight average of the standard deviations in the comparison groups, weighted by the respective sample sizes.  

Now the test statistic:

• Step 5. Conclusion.  

We reject H 0 because 2.66 > 1.960. We have statistically significant evidence at α=0.05 to show that there is a difference in mean systolic blood pressures between men and women. The p-value is p < 0.010.  

Here again we find that there is a statistically significant difference in mean systolic blood pressures between men and women at p < 0.010. Notice that there is a very small difference in the sample means (128.2-126.5 = 1.7 units), but this difference is beyond what would be expected by chance. Is this a clinically meaningful difference? The large sample size in this example is driving the statistical significance. A 95% confidence interval for the difference in mean systolic blood pressures is: 1.7 + 1.26 or (0.44, 2.96). The confidence interval provides an assessment of the magnitude of the difference between means whereas the test of hypothesis and p-value provide an assessment of the statistical significance of the difference.  

Above we performed a study to evaluate a new drug designed to lower total cholesterol. The study involved one sample of patients, each patient took the new drug for 6 weeks and had their cholesterol measured. As a means of evaluating the efficacy of the new drug, the mean total cholesterol following 6 weeks of treatment was compared to the NCHS-reported mean total cholesterol level in 2002 for all adults of 203. At the end of the example, we discussed the appropriateness of the fixed comparator as well as an alternative study design to evaluate the effect of the new drug involving two treatment groups, where one group receives the new drug and the other does not. Here, we revisit the example with a concurrent or parallel control group, which is very typical in randomized controlled trials or clinical trials (refer to the EP713 module on Clinical Trials ).  

A new drug is proposed to lower total cholesterol. A randomized controlled trial is designed to evaluate the efficacy of the medication in lowering cholesterol. Thirty participants are enrolled in the trial and are randomly assigned to receive either the new drug or a placebo. The participants do not know which treatment they are assigned. Each participant is asked to take the assigned treatment for 6 weeks. At the end of 6 weeks, each patient's total cholesterol level is measured and the sample statistics are as follows.

Is there statistical evidence of a reduction in mean total cholesterol in patients taking the new drug for 6 weeks as compared to participants taking placebo? We will run the test using the five-step approach.

H 0 : μ 1 = μ 2 H 1 : μ 1 < μ 2                         α=0.05

Because both samples are small (< 30), we use the t test statistic. Before implementing the formula, we first check whether the assumption of equality of population variances is reasonable. The ratio of the sample variances, s 1 2 /s 2 2 =28.7 2 /30.3 2 = 0.90, which falls between 0.5 and 2, suggesting that the assumption of equality of population variances is reasonable. The appropriate test statistic is:

This is a lower-tailed test, using a t statistic and a 5% level of significance. The appropriate critical value can be found in the t Table (in More Resources to the right). In order to determine the critical value of t we need degrees of freedom, df, defined as df=n 1 +n 2 -2 = 15+15-2=28. The critical value for a lower tailed test with df=28 and α=0.05 is -1.701 and the decision rule is: Reject H 0 if t < -1.701.

Now the test statistic,

We reject H 0 because -2.92 < -1.701. We have statistically significant evidence at α=0.05 to show that the mean total cholesterol level is lower in patients taking the new drug for 6 weeks as compared to patients taking placebo, p < 0.005.

The clinical trial in this example finds a statistically significant reduction in total cholesterol, whereas in the previous example where we had a historical control (as opposed to a parallel control group) we did not demonstrate efficacy of the new drug. Notice that the mean total cholesterol level in patients taking placebo is 217.4 which is very different from the mean cholesterol reported among all Americans in 2002 of 203 and used as the comparator in the prior example. The historical control value may not have been the most appropriate comparator as cholesterol levels have been increasing over time. In the next section, we present another design that can be used to assess the efficacy of the new drug.

Video - Comparison of Two Independent Samples With a Continuous Outcome (8:02)

Link to transcript of the video

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Inference for Comparing 2 Population Means (HT for 2 Means, independent samples)

More of the good stuff! We will need to know how to label the null and alternative hypothesis, calculate the test statistic, and then reach our conclusion using the critical value method or the p-value method.

The Test Statistic for a Test of 2 Means from Independent Samples:

[latex]t = \displaystyle \frac{(\bar{x_1} - \bar{x_2}) - (\mu_1 - \mu_2)}{\sqrt{\displaystyle \frac{s_1^2}{n_1} + \displaystyle \frac{s_2^2}{n_2}}}[/latex]

What the different symbols mean:

[latex]n_1[/latex] is the sample size for the first group

[latex]n_2[/latex] is the sample size for the second group

[latex]df[/latex], the degrees of freedom, is the smaller of [latex]n_1 - 1[/latex] and [latex]n_2 - 1[/latex]

[latex]\mu_1[/latex] is the population mean from the first group

[latex]\mu_2[/latex] is the population mean from the second group

[latex]\bar{x_1}[/latex] is the sample mean for the first group

[latex]\bar{x_2}[/latex] is the sample mean for the second group

[latex]s_1[/latex] is the sample standard deviation for the first group

[latex]s_2[/latex] is the sample standard deviation for the second group

[latex]\alpha[/latex] is the significance level , usually given within the problem, or if not given, we assume it to be 5% or 0.05

Assumptions when conducting a Test for 2 Means from Independent Samples:

• We do not know the population standard deviations, and we do not assume they are equal
• The two samples or groups are independent
• Both samples are simple random samples
• Both populations are Normally distributed OR both samples are large ([latex]n_1 > 30[/latex] and [latex]n_2 > 30[/latex])

Steps to conduct the Test for 2 Means from Independent Samples:

• Identify all the symbols listed above (all the stuff that will go into the formulas). This includes [latex]n_1[/latex] and [latex]n_2[/latex], [latex]df[/latex], [latex]\mu_1[/latex] and [latex]\mu_2[/latex], [latex]\bar{x_1}[/latex] and [latex]\bar{x_2}[/latex], [latex]s_1[/latex] and [latex]s_2[/latex], and [latex]\alpha[/latex]
• Identify the null and alternative hypotheses
• Calculate the test statistic, [latex]t = \displaystyle \frac{(\bar{x_1} - \bar{x_2}) - (\mu_1 - \mu_2)}{\sqrt{\displaystyle \frac{s_1^2}{n_1} + \displaystyle \frac{s_2^2}{n_2}}}[/latex]
• Find the critical value(s) OR the p-value OR both
• Apply the Decision Rule
• Write up a conclusion for the test

Example 1: Study on the effectiveness of stents for stroke patients [1]

In this study , researchers randomly assigned stroke patients to two groups: one received the current standard care (control) and the other received a stent surgery in addition to the standard care (stent treatment). If the stents work, the treatment group should have a lower average disability score . Do the results give convincing statistical evidence that the stent treatment reduces the average disability from stroke?

Since we are being asked for convincing statistical evidence, a hypothesis test should be conducted. In this case, we are dealing with averages from two samples or groups (the patients with stent treatment and patients receiving the standard care), so we will conduct a Test of 2 Means.

• [latex]n_1 = 98[/latex] is the sample size for the first group
• [latex]n_2 = 93[/latex] is the sample size for the second group
• [latex]df[/latex], the degrees of freedom, is the smaller of [latex]98 - 1 = 97[/latex] and [latex]93 - 1 = 92[/latex], so [latex]df = 92[/latex]
• [latex]\bar{x_1} = 2.26[/latex] is the sample mean for the first group
• [latex]\bar{x_2} = 3.23[/latex] is the sample mean for the second group
• [latex]s_1 = 1.78[/latex] is the sample standard deviation for the first group
• [latex]s_2 = 1.78[/latex] is the sample standard deviation for the second group
• [latex]\alpha = 0.05[/latex] (we were not told a specific value in the problem, so we are assuming it is 5%)
• One additional assumption we extend from the null hypothesis is that [latex]\mu_1 - \mu_2 = 0[/latex]; this means that in our formula, those variables cancel out
• [latex]H_{0}: \mu_1 = \mu_2[/latex]
• [latex]H_{A}: \mu_1 < \mu_2[/latex]
• [latex]t = \displaystyle \frac{(\bar{x_1} - \bar{x_2}) - (\mu_1 - \mu_2)}{\sqrt{\displaystyle \frac{s_1^2}{n_1} + \displaystyle \frac{s_2^2}{n_2}}} = \displaystyle \frac{(2.26 - 3.23) - 0)}{\sqrt{\displaystyle \frac{1.78^2}{98} + \displaystyle \frac{1.78^2}{93}}} = -3.76[/latex]
• StatDisk : We can conduct this test using StatDisk. The nice thing about StatDisk is that it will also compute the test statistic. From the main menu above we click on Analysis, Hypothesis Testing, and then Mean Two Independent Samples. From there enter the 0.05 significance, along with the specific values as outlined in the picture below in Step 2. Notice the alternative hypothesis is the [latex]<[/latex] option. Enter the sample size, mean, and standard deviation for each group, and make sure that unequal variances is selected. Now we click on Evaluate. If you check the values, the test statistic is reported in the Step 3 display, as well as the P-Value of 0.00011.
• Applying the Decision Rule: We now compare this to our significance level, which is 0.05. If the p-value is smaller or equal to the alpha level, we have enough evidence for our claim, otherwise we do not. Here, [latex]p-value = 0.00011[/latex], which is definitely smaller than [latex]\alpha = 0.05[/latex], so we have enough evidence for the alternative hypothesis…but what does this mean?
• Conclusion: Because our p-value  of [latex]0.00011[/latex] is less than our [latex]\alpha[/latex] level of [latex]0.05[/latex], we reject [latex]H_{0}[/latex]. We have convincing statistical evidence that the stent treatment reduces the average disability from stroke.

Example 2: Home Run Distances

In 1998, Sammy Sosa and Mark McGwire (2 players in Major League Baseball) were on pace to set a new home run record. At the end of the season McGwire ended up with 70 home runs, and Sosa ended up with 66. The home run distances were recorded and compared (sometimes a player’s home run distance is used to measure their “power”). Do the results give convincing statistical evidence that the home run distances are different from each other? Who would you say “hit the ball farther” in this comparison?

Since we are being asked for convincing statistical evidence, a hypothesis test should be conducted. In this case, we are dealing with averages from two samples or groups (the home run distances), so we will conduct a Test of 2 Means.

• [latex]n_1 = 70[/latex] is the sample size for the first group
• [latex]n_2 = 66[/latex] is the sample size for the second group
• [latex]df[/latex], the degrees of freedom, is the smaller of [latex]70 - 1 = 69[/latex] and [latex]66 - 1 = 65[/latex], so [latex]df = 65[/latex]
• [latex]\bar{x_1} = 418.5[/latex] is the sample mean for the first group
• [latex]\bar{x_2} = 404.8[/latex] is the sample mean for the second group
• [latex]s_1 = 45.5[/latex] is the sample standard deviation for the first group
• [latex]s_2 = 35.7[/latex] is the sample standard deviation for the second group
• [latex]H_{A}: \mu_1 \neq \mu_2[/latex]
• [latex]t = \displaystyle \frac{(\bar{x_1} - \bar{x_2}) - (\mu_1 - \mu_2)}{\sqrt{\displaystyle \frac{s_1^2}{n_1} + \displaystyle \frac{s_2^2}{n_2}}} = \displaystyle \frac{(418.5 - 404.8) - 0)}{\sqrt{\displaystyle \frac{45.5^2}{70} + \displaystyle \frac{35.7^2}{65}}} = 1.95[/latex]
• StatDisk : We can conduct this test using StatDisk. The nice thing about StatDisk is that it will also compute the test statistic. From the main menu above we click on Analysis, Hypothesis Testing, and then Mean Two Independent Samples. From there enter the 0.05 significance, along with the specific values as outlined in the picture below in Step 2. Notice the alternative hypothesis is the [latex]\neq[/latex] option. Enter the sample size, mean, and standard deviation for each group, and make sure that unequal variances is selected. Now we click on Evaluate. If you check the values, the test statistic is reported in the Step 3 display, as well as the P-Value of 0.05221.
• Applying the Decision Rule: We now compare this to our significance level, which is 0.05. If the p-value is smaller or equal to the alpha level, we have enough evidence for our claim, otherwise we do not. Here, [latex]p-value = 0.05221[/latex], which is larger than [latex]\alpha = 0.05[/latex], so we do not have enough evidence for the alternative hypothesis…but what does this mean?
• Conclusion: Because our p-value  of [latex]0.05221[/latex] is larger than our [latex]\alpha[/latex] level of [latex]0.05[/latex], we fail to reject [latex]H_{0}[/latex]. We do not have convincing statistical evidence that the home run distances are different.
• Follow-up commentary: But what does this mean? There actually was a difference, right? If we take McGwire’s average and subtract Sosa’s average we get a difference of 13.7. What this result indicates is that the difference is not statistically significant; it could be due more to random chance than something meaningful. Other factors, such as sample size, could also be a determining factor (with a larger sample size, the difference may have been more meaningful).
• Adapted from the Skew The Script curriculum ( skewthescript.org ), licensed under CC BY-NC-Sa 4.0 ↵

Basic Statistics Copyright © by Allyn Leon is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License , except where otherwise noted.

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• SPSS Tutorials

Independent Samples t Test

Spss tutorials: independent samples t test.

• The SPSS Environment
• The Data View Window
• Using SPSS Syntax
• Data Creation in SPSS
• Importing Data into SPSS
• Variable Types
• Date-Time Variables in SPSS
• Defining Variables
• Creating a Codebook
• Computing Variables
• Computing Variables: Mean Centering
• Computing Variables: Recoding Categorical Variables
• Computing Variables: Recoding String Variables into Coded Categories (Automatic Recode)
• rank transform converts a set of data values by ordering them from smallest to largest, and then assigning a rank to each value. In SPSS, the Rank Cases procedure can be used to compute the rank transform of a variable." href="https://libguides.library.kent.edu/SPSS/RankCases" style="" >Computing Variables: Rank Transforms (Rank Cases)
• Weighting Cases
• Sorting Data
• Grouping Data
• Descriptive Stats for One Numeric Variable (Explore)
• Descriptive Stats for One Numeric Variable (Frequencies)
• Descriptive Stats for Many Numeric Variables (Descriptives)
• Descriptive Stats by Group (Compare Means)
• Frequency Tables
• Working with "Check All That Apply" Survey Data (Multiple Response Sets)
• Chi-Square Test of Independence
• Pearson Correlation
• One Sample t Test
• Paired Samples t Test
• One-Way ANOVA
• How to Cite the Tutorials

Sample Data Files

Our tutorials reference a dataset called "sample" in many examples. If you'd like to download the sample dataset to work through the examples, choose one of the files below:

• Data definitions (*.pdf)
• Data - Comma delimited (*.csv)
• Data - Tab delimited (*.txt)
• Data - Excel format (*.xlsx)
• Data - SAS format (*.sas7bdat)
• Data - SPSS format (*.sav)
• SPSS Syntax (*.sps) Syntax to add variable labels, value labels, set variable types, and compute several recoded variables used in later tutorials.
• SAS Syntax (*.sas) Syntax to read the CSV-format sample data and set variable labels and formats/value labels.

The Independent Samples t Test compares the means of two independent groups in order to determine whether there is statistical evidence that the associated population means are significantly different. The Independent Samples t Test is a parametric test.

This test is also known as:

• Independent t Test
• Independent Measures t Test
• Independent Two-sample t Test
• Student t Test
• Two-Sample t Test
• Uncorrelated Scores t Test
• Unpaired t Test
• Unrelated t Test

The variables used in this test are known as:

• Dependent variable, or test variable
• Independent variable, or grouping variable

Common Uses

The Independent Samples t Test is commonly used to test the following:

• Statistical differences between the means of two groups
• Statistical differences between the means of two interventions
• Statistical differences between the means of two change scores

Note:  The Independent Samples  t  Test can only compare the means for two (and only two) groups. It cannot make comparisons among more than two groups. If you wish to compare the means across more than two groups, you will likely want to run an ANOVA.

Data Requirements

Your data must meet the following requirements:

• Dependent variable that is continuous (i.e., interval or ratio level)
• Independent variable that is categorical and has exactly two categories
• Cases that have values on both the dependent and independent variables
• Subjects in the first group cannot also be in the second group
• No subject in either group can influence subjects in the other group
• No group can influence the other group
• Violation of this assumption will yield an inaccurate p value
• Random sample of data from the population
• Non-normal population distributions, especially those that are thick-tailed or heavily skewed, considerably reduce the power of the test
• Among moderate or large samples, a violation of normality may still yield accurate p values
• When this assumption is violated and the sample sizes for each group differ, the p value is not trustworthy. However, the Independent Samples t Test output also includes an approximate t statistic that is not based on assuming equal population variances. This alternative statistic, called the Welch t Test statistic 1 , may be used when equal variances among populations cannot be assumed. The Welch t Test is also known an Unequal Variance t Test or Separate Variances t Test.
• No outliers

Note: When one or more of the assumptions for the Independent Samples t Test are not met, you may want to run the nonparametric Mann-Whitney U Test instead.

Researchers often follow several rules of thumb:

• Each group should have at least 6 subjects, ideally more. Inferences for the population will be more tenuous with too few subjects.
• A balanced design (i.e., same number of subjects in each group) is ideal. Extremely unbalanced designs increase the possibility that violating any of the requirements/assumptions will threaten the validity of the Independent Samples t Test.

1  Welch, B. L. (1947). The generalization of "Student's" problem when several different population variances are involved. Biometrika , 34 (1–2), 28–35.

The null hypothesis ( H 0 ) and alternative hypothesis ( H 1 ) of the Independent Samples t Test can be expressed in two different but equivalent ways:

H 0 : µ 1  = µ 2 ("the two population means are equal") H 1 : µ 1  ≠ µ 2 ("the two population means are not equal")

H 0 : µ 1  - µ 2  = 0 ("the difference between the two population means is equal to 0") H 1 :  µ 1  - µ 2  ≠ 0 ("the difference between the two population means is not 0")

where µ 1 and µ 2 are the population means for group 1 and group 2, respectively. Notice that the second set of hypotheses can be derived from the first set by simply subtracting µ 2 from both sides of the equation.

Levene’s Test for Equality of Variances

Recall that the Independent Samples t Test requires the assumption of homogeneity of variance -- i.e., both groups have the same variance. SPSS conveniently includes a test for the homogeneity of variance, called Levene's Test , whenever you run an independent samples t test.

The hypotheses for Levene’s test are: 

H 0 : σ 1 2 - σ 2 2 = 0 ("the population variances of group 1 and 2 are equal") H 1 : σ 1 2 - σ 2 2 ≠ 0 ("the population variances of group 1 and 2 are not equal")

This implies that if we reject the null hypothesis of Levene's Test, it suggests that the variances of the two groups are not equal; i.e., that the homogeneity of variances assumption is violated.

The output in the Independent Samples Test table includes two rows: Equal variances assumed and Equal variances not assumed . If Levene’s test indicates that the variances are equal across the two groups (i.e., p -value large), you will rely on the first row of output, Equal variances assumed , when you look at the results for the actual Independent Samples t Test (under the heading t -test for Equality of Means). If Levene’s test indicates that the variances are not equal across the two groups (i.e., p -value small), you will need to rely on the second row of output, Equal variances not assumed , when you look at the results of the Independent Samples t Test (under the heading t -test for Equality of Means). 

The difference between these two rows of output lies in the way the independent samples t test statistic is calculated. When equal variances are assumed, the calculation uses pooled variances; when equal variances cannot be assumed, the calculation utilizes un-pooled variances and a correction to the degrees of freedom.

Test Statistic

The test statistic for an Independent Samples t Test is denoted t . There are actually two forms of the test statistic for this test, depending on whether or not equal variances are assumed. SPSS produces both forms of the test, so both forms of the test are described here. Note that the null and alternative hypotheses are identical for both forms of the test statistic.

Equal variances assumed

When the two independent samples are assumed to be drawn from populations with identical population variances (i.e., σ 1 2 = σ 2 2 ) , the test statistic t is computed as:

$$ t = \frac{\overline{x}_{1} - \overline{x}_{2}}{s_{p}\sqrt{\frac{1}{n_{1}} + \frac{1}{n_{2}}}} $$

$$ s_{p} = \sqrt{\frac{(n_{1} - 1)s_{1}^{2} + (n_{2} - 1)s_{2}^{2}}{n_{1} + n_{2} - 2}} $$

\(\bar{x}_{1}\) = Mean of first sample \(\bar{x}_{2}\) = Mean of second sample \(n_{1}\) = Sample size (i.e., number of observations) of first sample \(n_{2}\) = Sample size (i.e., number of observations) of second sample \(s_{1}\) = Standard deviation of first sample \(s_{2}\) = Standard deviation of second sample \(s_{p}\) = Pooled standard deviation

The calculated t value is then compared to the critical t value from the t distribution table with degrees of freedom df = n 1 + n 2 - 2 and chosen confidence level. If the calculated t value is greater than the critical t value, then we reject the null hypothesis.

Note that this form of the independent samples t test statistic assumes equal variances.

Because we assume equal population variances, it is OK to "pool" the sample variances ( s p ). However, if this assumption is violated, the pooled variance estimate may not be accurate, which would affect the accuracy of our test statistic (and hence, the p-value).

Equal variances not assumed

When the two independent samples are assumed to be drawn from populations with unequal variances (i.e., σ 1 2  ≠ σ 2 2 ), the test statistic t is computed as:

$$ t = \frac{\overline{x}_{1} - \overline{x}_{2}}{\sqrt{\frac{s_{1}^{2}}{n_{1}} + \frac{s_{2}^{2}}{n_{2}}}} $$

\(\bar{x}_{1}\) = Mean of first sample \(\bar{x}_{2}\) = Mean of second sample \(n_{1}\) = Sample size (i.e., number of observations) of first sample \(n_{2}\) = Sample size (i.e., number of observations) of second sample \(s_{1}\) = Standard deviation of first sample \(s_{2}\) = Standard deviation of second sample

The calculated t value is then compared to the critical t value from the t distribution table with degrees of freedom

$$ df = \frac{ \left ( \frac{s_{1}^2}{n_{1}} + \frac{s_{2}^2}{n_{2}} \right ) ^{2} }{ \frac{1}{n_{1}-1} \left ( \frac{s_{1}^2}{n_{1}} \right ) ^{2} + \frac{1}{n_{2}-1} \left ( \frac{s_{2}^2}{n_{2}} \right ) ^{2}} $$

and chosen confidence level. If the calculated t value > critical t value, then we reject the null hypothesis.

Note that this form of the independent samples t test statistic does not assume equal variances. This is why both the denominator of the test statistic and the degrees of freedom of the critical value of  t are different than the equal variances form of the test statistic.

Data Set-Up

Your data should include two variables (represented in columns) that will be used in the analysis. The independent variable should be categorical and include exactly two groups. (Note that SPSS restricts categorical indicators to numeric or short string values only.) The dependent variable should be continuous (i.e., interval or ratio). SPSS can only make use of cases that have nonmissing values for the independent and the dependent variables, so if a case has a missing value for either variable, it cannot be included in the test.

The number of rows in the dataset should correspond to the number of subjects in the study. Each row of the dataset should represent a unique subject, person, or unit, and all of the measurements taken on that person or unit should appear in that row.

Run an Independent Samples t Test

To run an Independent Samples t Test in SPSS, click  Analyze > Compare Means > Independent-Samples T Test .

The Independent-Samples T Test window opens where you will specify the variables to be used in the analysis. All of the variables in your dataset appear in the list on the left side. Move variables to the right by selecting them in the list and clicking the blue arrow buttons. You can move a variable(s) to either of two areas: Grouping Variable or Test Variable(s) .

A Test Variable(s): The dependent variable(s). This is the continuous variable whose means will be compared between the two groups. You may run multiple t tests simultaneously by selecting more than one test variable.

B Grouping Variable: The independent variable. The categories (or groups) of the independent variable will define which samples will be compared in the t test. The grouping variable must have at least two categories (groups); it may have more than two categories but a t test can only compare two groups, so you will need to specify which two groups to compare. You can also use a continuous variable by specifying a cut point to create two groups (i.e., values at or above the cut point and values below the cut point).

C Define Groups : Click Define Groups to define the category indicators (groups) to use in the t test. If the button is not active, make sure that you have already moved your independent variable to the right in the Grouping Variable field. You must define the categories of your grouping variable before you can run the Independent Samples t Test procedure.

You will not be able to run the Independent Samples t Test until the levels (or cut points) of the grouping variable have been defined. The OK and Paste buttons will be unclickable until the levels have been defined. You can tell if the levels of the grouping variable have not been defined by looking at the Grouping Variable box: if a variable appears in the box but has two question marks next to it, then the levels are not defined:

D Options: The Options section is where you can set your desired confidence level for the confidence interval for the mean difference, and specify how SPSS should handle missing values.

When finished, click OK to run the Independent Samples t Test, or click Paste to have the syntax corresponding to your specified settings written to an open syntax window. (If you do not have a syntax window open, a new window will open for you.)

Define Groups

Clicking the Define Groups button (C) opens the Define Groups window:

1 Use specified values: If your grouping variable is categorical, select Use specified values . Enter the values for the categories you wish to compare in the Group 1 and Group 2 fields. If your categories are numerically coded, you will enter the numeric codes. If your group variable is string, you will enter the exact text strings representing the two categories. If your grouping variable has more than two categories (e.g., takes on values of 1, 2, 3, 4), you can specify two of the categories to be compared (SPSS will disregard the other categories in this case).

Note that when computing the test statistic, SPSS will subtract the mean of the Group 2 from the mean of Group 1. Changing the order of the subtraction affects the sign of the results, but does not affect the magnitude of the results.

2 Cut point: If your grouping variable is numeric and continuous, you can designate a cut point for dichotomizing the variable. This will separate the cases into two categories based on the cut point. Specifically, for a given cut point x , the new categories will be:

• Group 1: All cases where grouping variable > x
• Group 2: All cases where grouping variable < x

Note that this implies that cases where the grouping variable is equal to the cut point itself will be included in the "greater than or equal to" category. (If you want your cut point to be included in a "less than or equal to" group, then you will need to use Recode into Different Variables or use DO IF syntax to create this grouping variable yourself.) Also note that while you can use cut points on any variable that has a numeric type, it may not make practical sense depending on the actual measurement level of the variable (e.g., nominal categorical variables coded numerically). Additionally, using a dichotomized variable created via a cut point generally reduces the power of the test compared to using a non-dichotomized variable.

Clicking the Options button (D) opens the Options window:

The Confidence Interval Percentage box allows you to specify the confidence level for a confidence interval. Note that this setting does NOT affect the test statistic or p-value or standard error; it only affects the computed upper and lower bounds of the confidence interval. You can enter any value between 1 and 99 in this box (although in practice, it only makes sense to enter numbers between 90 and 99).

The Missing Values section allows you to choose if cases should be excluded "analysis by analysis" (i.e. pairwise deletion) or excluded listwise. This setting is not relevant if you have only specified one dependent variable; it only matters if you are entering more than one dependent (continuous numeric) variable. In that case, excluding "analysis by analysis" will use all nonmissing values for a given variable. If you exclude "listwise", it will only use the cases with nonmissing values for all of the variables entered. Depending on the amount of missing data you have, listwise deletion could greatly reduce your sample size.

Example: Independent samples T test when variances are not equal

Problem statement.

In our sample dataset, students reported their typical time to run a mile, and whether or not they were an athlete. Suppose we want to know if the average time to run a mile is different for athletes versus non-athletes. This involves testing whether the sample means for mile time among athletes and non-athletes in your sample are statistically different (and by extension, inferring whether the means for mile times in the population are significantly different between these two groups). You can use an Independent Samples t Test to compare the mean mile time for athletes and non-athletes.

The hypotheses for this example can be expressed as:

H 0 : µ non-athlete  − µ athlete  = 0 ("the difference of the means is equal to zero") H 1 : µ non-athlete  − µ athlete  ≠ 0 ("the difference of the means is not equal to zero")

where µ athlete and µ non-athlete are the population means for athletes and non-athletes, respectively.

In the sample data, we will use two variables: Athlete and MileMinDur . The variable Athlete has values of either “0” (non-athlete) or "1" (athlete). It will function as the independent variable in this T test. The variable MileMinDur is a numeric duration variable (h:mm:ss), and it will function as the dependent variable. In SPSS, the first few rows of data look like this:

Before the Test

Before running the Independent Samples t Test, it is a good idea to look at descriptive statistics and graphs to get an idea of what to expect. Running Compare Means ( Analyze > Compare Means > Means ) to get descriptive statistics by group tells us that the standard deviation in mile time for non-athletes is about 2 minutes; for athletes, it is about 49 seconds. This corresponds to a variance of 14803 seconds for non-athletes, and a variance of 2447 seconds for athletes 1 . Running the Explore procedure ( Analyze > Descriptives > Explore ) to obtain a comparative boxplot yields the following graph:

If the variances were indeed equal, we would expect the total length of the boxplots to be about the same for both groups. However, from this boxplot, it is clear that the spread of observations for non-athletes is much greater than the spread of observations for athletes. Already, we can estimate that the variances for these two groups are quite different. It should not come as a surprise if we run the Independent Samples t Test and see that Levene's Test is significant.

Additionally, we should also decide on a significance level (typically denoted using the Greek letter alpha, α ) before we perform our hypothesis tests. The significance level is the threshold we use to decide whether a test result is significant. For this example, let's use α = 0.05.

1 When computing the variance of a duration variable (formatted as hh:mm:ss or mm:ss or mm:ss.s), SPSS converts the standard deviation value to seconds before squaring.

Running the Test

To run the Independent Samples t Test:

• Click  Analyze > Compare Means > Independent-Samples T Test .
• Move the variable Athlete to the Grouping Variable field, and move the variable MileMinDur to the Test Variable(s) area. Now Athlete is defined as the independent variable and MileMinDur is defined as the dependent variable.
• Click Define Groups , which opens a new window. Use specified values is selected by default. Since our grouping variable is numerically coded (0 = "Non-athlete", 1 = "Athlete"), type “0” in the first text box, and “1” in the second text box. This indicates that we will compare groups 0 and 1, which correspond to non-athletes and athletes, respectively. Click Continue when finished.
• Click OK to run the Independent Samples t Test. Output for the analysis will display in the Output Viewer window. 

Two sections (boxes) appear in the output: Group Statistics and Independent Samples Test . The first section, Group Statistics , provides basic information about the group comparisons, including the sample size ( n ), mean, standard deviation, and standard error for mile times by group. In this example, there are 166 athletes and 226 non-athletes. The mean mile time for athletes is 6 minutes 51 seconds, and the mean mile time for non-athletes is 9 minutes 6 seconds.

The second section, Independent Samples Test , displays the results most relevant to the Independent Samples t Test. There are two parts that provide different pieces of information: (A) Levene’s Test for Equality of Variances and (B) t-test for Equality of Means.

A Levene's Test for Equality of of Variances : This section has the test results for Levene's Test. From left to right:

• F is the test statistic of Levene's test
• Sig. is the p-value corresponding to this test statistic.

The p -value of Levene's test is printed as ".000" (but should be read as p < 0.001 -- i.e., p very small), so we we reject the null of Levene's test and conclude that the variance in mile time of athletes is significantly different than that of non-athletes. This tells us that we should look at the "Equal variances not assumed" row for the t test (and corresponding confidence interval) results . (If this test result had not been significant -- that is, if we had observed p > α -- then we would have used the "Equal variances assumed" output.)

B t-test for Equality of Means provides the results for the actual Independent Samples t Test. From left to right:

• t is the computed test statistic, using the formula for the equal-variances-assumed test statistic (first row of table) or the formula for the equal-variances-not-assumed test statistic (second row of table)
• df is the degrees of freedom, using the equal-variances-assumed degrees of freedom formula (first row of table) or the equal-variances-not-assumed degrees of freedom formula (second row of table)
• Sig (2-tailed) is the p-value corresponding to the given test statistic and degrees of freedom
• Mean Difference is the difference between the sample means, i.e. x 1  − x 2 ; it also corresponds to the numerator of the test statistic for that test
• Std. Error Difference is the standard error of the mean difference estimate; it also corresponds to the denominator of the test statistic for that test

Note that the mean difference is calculated by subtracting the mean of the second group from the mean of the first group. In this example, the mean mile time for athletes was subtracted from the mean mile time for non-athletes (9:06 minus 6:51 = 02:14). The sign of the mean difference corresponds to the sign of the t value. The positive t value in this example indicates that the mean mile time for the first group, non-athletes, is significantly greater than the mean for the second group, athletes.

The associated p value is printed as ".000"; double-clicking on the p-value will reveal the un-rounded number. SPSS rounds p-values to three decimal places, so any p-value too small to round up to .001 will print as .000. (In this particular example, the p-values are on the order of 10 -40 .)

C Confidence Interval of the Difference : This part of the t -test output complements the significance test results. Typically, if the CI for the mean difference contains 0 within the interval -- i.e., if the lower boundary of the CI is a negative number and the upper boundary of the CI is a positive number -- the results are not significant at the chosen significance level. In this example, the 95% CI is [01:57, 02:32], which does not contain zero; this agrees with the small p -value of the significance test.

Decision and Conclusions

Since p < .001 is less than our chosen significance level α = 0.05, we can reject the null hypothesis, and conclude that the that the mean mile time for athletes and non-athletes is significantly different.

Based on the results, we can state the following:

• There was a significant difference in mean mile time between non-athletes and athletes ( t 315.846 = 15.047, p < .001).
• The average mile time for athletes was 2 minutes and 14 seconds lower than the average mile time for non-athletes.
• << Previous: Paired Samples t Test
• Next: One-Way ANOVA >>
• Last Updated: May 10, 2024 1:32 PM
• URL: https://libguides.library.kent.edu/SPSS

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t-test Calculator

Table of contents

Welcome to our t-test calculator! Here you can not only easily perform one-sample t-tests , but also two-sample t-tests , as well as paired t-tests .

Do you prefer to find the p-value from t-test, or would you rather find the t-test critical values? Well, this t-test calculator can do both! 😊

What does a t-test tell you? Take a look at the text below, where we explain what actually gets tested when various types of t-tests are performed. Also, we explain when to use t-tests (in particular, whether to use the z-test vs. t-test) and what assumptions your data should satisfy for the results of a t-test to be valid. If you've ever wanted to know how to do a t-test by hand, we provide the necessary t-test formula, as well as tell you how to determine the number of degrees of freedom in a t-test.

When to use a t-test?

A t-test is one of the most popular statistical tests for location , i.e., it deals with the population(s) mean value(s).

There are different types of t-tests that you can perform:

• A one-sample t-test;
• A two-sample t-test; and
• A paired t-test.

In the next section , we explain when to use which. Remember that a t-test can only be used for one or two groups . If you need to compare three (or more) means, use the analysis of variance ( ANOVA ) method.

The t-test is a parametric test, meaning that your data has to fulfill some assumptions :

• The data points are independent; AND
• The data, at least approximately, follow a normal distribution .

If your sample doesn't fit these assumptions, you can resort to nonparametric alternatives. Visit our Mann–Whitney U test calculator or the Wilcoxon rank-sum test calculator to learn more. Other possibilities include the Wilcoxon signed-rank test or the sign test.

Which t-test?

Your choice of t-test depends on whether you are studying one group or two groups:

One sample t-test

Choose the one-sample t-test to check if the mean of a population is equal to some pre-set hypothesized value .

The average volume of a drink sold in 0.33 l cans — is it really equal to 330 ml?

The average weight of people from a specific city — is it different from the national average?

Two-sample t-test

Choose the two-sample t-test to check if the difference between the means of two populations is equal to some pre-determined value when the two samples have been chosen independently of each other.

In particular, you can use this test to check whether the two groups are different from one another .

The average difference in weight gain in two groups of people: one group was on a high-carb diet and the other on a high-fat diet.

The average difference in the results of a math test from students at two different universities.

This test is sometimes referred to as an independent samples t-test , or an unpaired samples t-test .

Paired t-test

A paired t-test is used to investigate the change in the mean of a population before and after some experimental intervention , based on a paired sample, i.e., when each subject has been measured twice: before and after treatment.

In particular, you can use this test to check whether, on average, the treatment has had any effect on the population .

The change in student test performance before and after taking a course.

The change in blood pressure in patients before and after administering some drug.

How to do a t-test?

So, you've decided which t-test to perform. These next steps will tell you how to calculate the p-value from t-test or its critical values, and then which decision to make about the null hypothesis.

Decide on the alternative hypothesis :

Use a two-tailed t-test if you only care whether the population's mean (or, in the case of two populations, the difference between the populations' means) agrees or disagrees with the pre-set value.

Use a one-tailed t-test if you want to test whether this mean (or difference in means) is greater/less than the pre-set value.

Compute your T-score value :

Formulas for the test statistic in t-tests include the sample size , as well as its mean and standard deviation . The exact formula depends on the t-test type — check the sections dedicated to each particular test for more details.

Determine the degrees of freedom for the t-test:

The degrees of freedom are the number of observations in a sample that are free to vary as we estimate statistical parameters. In the simplest case, the number of degrees of freedom equals your sample size minus the number of parameters you need to estimate . Again, the exact formula depends on the t-test you want to perform — check the sections below for details.

The degrees of freedom are essential, as they determine the distribution followed by your T-score (under the null hypothesis). If there are d degrees of freedom, then the distribution of the test statistics is the t-Student distribution with d degrees of freedom . This distribution has a shape similar to N(0,1) (bell-shaped and symmetric) but has heavier tails . If the number of degrees of freedom is large (>30), which generically happens for large samples, the t-Student distribution is practically indistinguishable from N(0,1).

💡 The t-Student distribution owes its name to William Sealy Gosset, who, in 1908, published his paper on the t-test under the pseudonym "Student". Gosset worked at the famous Guinness Brewery in Dublin, Ireland, and devised the t-test as an economical way to monitor the quality of beer. Cheers! 🍺🍺🍺

p-value from t-test

Recall that the p-value is the probability (calculated under the assumption that the null hypothesis is true) that the test statistic will produce values at least as extreme as the T-score produced for your sample . As probabilities correspond to areas under the density function, p-value from t-test can be nicely illustrated with the help of the following pictures:

The following formulae say how to calculate p-value from t-test. By cdf t,d we denote the cumulative distribution function of the t-Student distribution with d degrees of freedom:

p-value from left-tailed t-test:

p-value = cdf t,d (t score )

p-value from right-tailed t-test:

p-value = 1 − cdf t,d (t score )

p-value from two-tailed t-test:

p-value = 2 × cdf t,d (−|t score |)

or, equivalently: p-value = 2 − 2 × cdf t,d (|t score |)

However, the cdf of the t-distribution is given by a somewhat complicated formula. To find the p-value by hand, you would need to resort to statistical tables, where approximate cdf values are collected, or to specialized statistical software. Fortunately, our t-test calculator determines the p-value from t-test for you in the blink of an eye!

t-test critical values

Recall, that in the critical values approach to hypothesis testing, you need to set a significance level, α, before computing the critical values , which in turn give rise to critical regions (a.k.a. rejection regions).

Formulas for critical values employ the quantile function of t-distribution, i.e., the inverse of the cdf :

Critical value for left-tailed t-test: cdf t,d -1 (α)

critical region:

(-∞, cdf t,d -1 (α)]

Critical value for right-tailed t-test: cdf t,d -1 (1-α)

[cdf t,d -1 (1-α), ∞)

Critical values for two-tailed t-test: ±cdf t,d -1 (1-α/2)

(-∞, -cdf t,d -1 (1-α/2)] ∪ [cdf t,d -1 (1-α/2), ∞)

To decide the fate of the null hypothesis, just check if your T-score lies within the critical region:

If your T-score belongs to the critical region , reject the null hypothesis and accept the alternative hypothesis.

If your T-score is outside the critical region , then you don't have enough evidence to reject the null hypothesis.

How to use our t-test calculator

Choose the type of t-test you wish to perform:

A one-sample t-test (to test the mean of a single group against a hypothesized mean);

A two-sample t-test (to compare the means for two groups); or

A paired t-test (to check how the mean from the same group changes after some intervention).

Two-tailed;

Left-tailed; or

Right-tailed.

This t-test calculator allows you to use either the p-value approach or the critical regions approach to hypothesis testing!

Enter your T-score and the number of degrees of freedom . If you don't know them, provide some data about your sample(s): sample size, mean, and standard deviation, and our t-test calculator will compute the T-score and degrees of freedom for you .

Once all the parameters are present, the p-value, or critical region, will immediately appear underneath the t-test calculator, along with an interpretation!

One-sample t-test

The null hypothesis is that the population mean is equal to some value μ 0 \mu_0 μ 0 ​ .

The alternative hypothesis is that the population mean is:

• different from μ 0 \mu_0 μ 0 ​ ;
• smaller than μ 0 \mu_0 μ 0 ​ ; or
• greater than μ 0 \mu_0 μ 0 ​ .

One-sample t-test formula :

• μ 0 \mu_0 μ 0 ​ — Mean postulated in the null hypothesis;
• n n n — Sample size;
• x ˉ \bar{x} x ˉ — Sample mean; and
• s s s — Sample standard deviation.

Number of degrees of freedom in t-test (one-sample) = n − 1 n-1 n − 1 .

The null hypothesis is that the actual difference between these groups' means, μ 1 \mu_1 μ 1 ​ , and μ 2 \mu_2 μ 2 ​ , is equal to some pre-set value, Δ \Delta Δ .

The alternative hypothesis is that the difference μ 1 − μ 2 \mu_1 - \mu_2 μ 1 ​ − μ 2 ​ is:

• Different from Δ \Delta Δ ;
• Smaller than Δ \Delta Δ ; or
• Greater than Δ \Delta Δ .

In particular, if this pre-determined difference is zero ( Δ = 0 \Delta = 0 Δ = 0 ):

The null hypothesis is that the population means are equal.

The alternate hypothesis is that the population means are:

• μ 1 \mu_1 μ 1 ​ and μ 2 \mu_2 μ 2 ​ are different from one another;
• μ 1 \mu_1 μ 1 ​ is smaller than μ 2 \mu_2 μ 2 ​ ; and
• μ 1 \mu_1 μ 1 ​ is greater than μ 2 \mu_2 μ 2 ​ .

Formally, to perform a t-test, we should additionally assume that the variances of the two populations are equal (this assumption is called the homogeneity of variance ).

There is a version of a t-test that can be applied without the assumption of homogeneity of variance: it is called a Welch's t-test . For your convenience, we describe both versions.

Two-sample t-test if variances are equal

Use this test if you know that the two populations' variances are the same (or very similar).

Two-sample t-test formula (with equal variances) :

where s p s_p s p ​ is the so-called pooled standard deviation , which we compute as:

• Δ \Delta Δ — Mean difference postulated in the null hypothesis;
• n 1 n_1 n 1 ​ — First sample size;
• x ˉ 1 \bar{x}_1 x ˉ 1 ​ — Mean for the first sample;
• s 1 s_1 s 1 ​ — Standard deviation in the first sample;
• n 2 n_2 n 2 ​ — Second sample size;
• x ˉ 2 \bar{x}_2 x ˉ 2 ​ — Mean for the second sample; and
• s 2 s_2 s 2 ​ — Standard deviation in the second sample.

Number of degrees of freedom in t-test (two samples, equal variances) = n 1 + n 2 − 2 n_1 + n_2 - 2 n 1 ​ + n 2 ​ − 2 .

Two-sample t-test if variances are unequal (Welch's t-test)

Use this test if the variances of your populations are different.

Two-sample Welch's t-test formula if variances are unequal:

• s 1 s_1 s 1 ​ — Standard deviation in the first sample;
• s 2 s_2 s 2 ​ — Standard deviation in the second sample.

The number of degrees of freedom in a Welch's t-test (two-sample t-test with unequal variances) is very difficult to count. We can approximate it with the help of the following Satterthwaite formula :

Alternatively, you can take the smaller of n 1 − 1 n_1 - 1 n 1 ​ − 1 and n 2 − 1 n_2 - 1 n 2 ​ − 1 as a conservative estimate for the number of degrees of freedom.

🔎 The Satterthwaite formula for the degrees of freedom can be rewritten as a scaled weighted harmonic mean of the degrees of freedom of the respective samples: n 1 − 1 n_1 - 1 n 1 ​ − 1 and n 2 − 1 n_2 - 1 n 2 ​ − 1 , and the weights are proportional to the standard deviations of the corresponding samples.

As we commonly perform a paired t-test when we have data about the same subjects measured twice (before and after some treatment), let us adopt the convention of referring to the samples as the pre-group and post-group.

The null hypothesis is that the true difference between the means of pre- and post-populations is equal to some pre-set value, Δ \Delta Δ .

The alternative hypothesis is that the actual difference between these means is:

Typically, this pre-determined difference is zero. We can then reformulate the hypotheses as follows:

The null hypothesis is that the pre- and post-means are the same, i.e., the treatment has no impact on the population .

The alternative hypothesis:

• The pre- and post-means are different from one another (treatment has some effect);
• The pre-mean is smaller than the post-mean (treatment increases the result); or
• The pre-mean is greater than the post-mean (treatment decreases the result).

Paired t-test formula

In fact, a paired t-test is technically the same as a one-sample t-test! Let us see why it is so. Let x 1 , . . . , x n x_1, ... , x_n x 1 ​ , ... , x n ​ be the pre observations and y 1 , . . . , y n y_1, ... , y_n y 1 ​ , ... , y n ​ the respective post observations. That is, x i , y i x_i, y_i x i ​ , y i ​ are the before and after measurements of the i -th subject.

For each subject, compute the difference, d i : = x i − y i d_i := x_i - y_i d i ​ := x i ​ − y i ​ . All that happens next is just a one-sample t-test performed on the sample of differences d 1 , . . . , d n d_1, ... , d_n d 1 ​ , ... , d n ​ . Take a look at the formula for the T-score :

Δ \Delta Δ — Mean difference postulated in the null hypothesis;

n n n — Size of the sample of differences, i.e., the number of pairs;

x ˉ \bar{x} x ˉ — Mean of the sample of differences; and

s s s  — Standard deviation of the sample of differences.

Number of degrees of freedom in t-test (paired): n − 1 n - 1 n − 1

t-test vs Z-test

We use a Z-test when we want to test the population mean of a normally distributed dataset, which has a known population variance . If the number of degrees of freedom is large, then the t-Student distribution is very close to N(0,1).

Hence, if there are many data points (at least 30), you may swap a t-test for a Z-test, and the results will be almost identical. However, for small samples with unknown variance, remember to use the t-test because, in such cases, the t-Student distribution differs significantly from the N(0,1)!

🙋 Have you concluded you need to perform the z-test? Head straight to our z-test calculator !

What is a t-test?

A t-test is a widely used statistical test that analyzes the means of one or two groups of data. For instance, a t-test is performed on medical data to determine whether a new drug really helps.

What are different types of t-tests?

Different types of t-tests are:

• One-sample t-test;
• Two-sample t-test; and
• Paired t-test.

How to find the t value in a one sample t-test?

To find the t-value:

• Subtract the null hypothesis mean from the sample mean value.
• Divide the difference by the standard deviation of the sample.
• Multiply the resultant with the square root of the sample size.

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t-test for the population mean, μ, based on one independent sample . Null hypothesis H 0 : μ = μ 0  

Alternative hypothesis H 1

Test details

Significance level α

The probability that we reject a true H 0 (type I error).

Degrees of freedom

Calculated as sample size minus one.

Test results

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9.2: Comparing Two Independent Population Means (Hypothesis test)

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• The two independent samples are simple random samples from two distinct populations.
• if the sample sizes are small, the distributions are important (should be normal)
• if the sample sizes are large, the distributions are not important (need not be normal)

The test comparing two independent population means with unknown and possibly unequal population standard deviations is called the Aspin-Welch \(t\)-test. The degrees of freedom formula was developed by Aspin-Welch.

The comparison of two population means is very common. A difference between the two samples depends on both the means and the standard deviations. Very different means can occur by chance if there is great variation among the individual samples. In order to account for the variation, we take the difference of the sample means, \(\bar{X}_{1} - \bar{X}_{2}\), and divide by the standard error in order to standardize the difference. The result is a t-score test statistic.

Because we do not know the population standard deviations, we estimate them using the two sample standard deviations from our independent samples. For the hypothesis test, we calculate the estimated standard deviation, or standard error , of the difference in sample means , \(\bar{X}_{1} - \bar{X}_{2}\).

The standard error is:

\[\sqrt{\dfrac{(s_{1})^{2}}{n_{1}} + \dfrac{(s_{2})^{2}}{n_{2}}}\]

The test statistic ( t -score) is calculated as follows:

\[\dfrac{(\bar{x}-\bar{x}) - (\mu_{1} - \mu_{2})}{\sqrt{\dfrac{(s_{1})^{2}}{n_{1}} + \dfrac{(s_{2})^{2}}{n_{2}}}}\]

• \(s_{1}\) and \(s_{2}\), the sample standard deviations, are estimates of \(\sigma_{1}\) and \(\sigma_{1}\), respectively.
• \(\sigma_{1}\) and \(\sigma_{2}\) are the unknown population standard deviations.
• \(\bar{x}_{1}\) and \(\bar{x}_{2}\) are the sample means. \(\mu_{1}\) and \(\mu_{2}\) are the population means.

The number of degrees of freedom (\(df\)) requires a somewhat complicated calculation. However, a computer or calculator calculates it easily. The \(df\) are not always a whole number. The test statistic calculated previously is approximated by the Student's t -distribution with \(df\) as follows:

Degrees of freedom

\[df = \dfrac{\left(\dfrac{(s_{1})^{2}}{n_{1}} + \dfrac{(s_{2})^{2}}{n_{2}}\right)^{2}}{\left(\dfrac{1}{n_{1}-1}\right)\left(\dfrac{(s_{1})^{2}}{n_{1}}\right)^{2} + \left(\dfrac{1}{n_{2}-1}\right)\left(\dfrac{(s_{2})^{2}}{n_{2}}\right)^{2}}\]

We can also use a conservative estimation of degree of freedom by taking DF to be the smallest of \(n_{1}-1\) and \(n_{2}-1\)

When both sample sizes \(n_{1}\) and \(n_{2}\) are five or larger, the Student's t approximation is very good. Notice that the sample variances \((s_{1})^{2}\) and \((s_{2})^{2}\) are not pooled. (If the question comes up, do not pool the variances.)

It is not necessary to compute the degrees of freedom by hand. A calculator or computer easily computes it.

Example \(\PageIndex{1}\): Independent groups

The average amount of time boys and girls aged seven to 11 spend playing sports each day is believed to be the same. A study is done and data are collected, resulting in the data in Table \(\PageIndex{1}\). Each populations has a normal distribution.

Is there a difference in the mean amount of time boys and girls aged seven to 11 play sports each day? Test at the 5% level of significance.

The population standard deviations are not known. Let g be the subscript for girls and b be the subscript for boys. Then, \(\mu_{g}\) is the population mean for girls and \(\mu_{b}\) is the population mean for boys. This is a test of two independent groups, two population means.

Random variable: \(\bar{X}_{g} - \bar{X}_{b} =\) difference in the sample mean amount of time girls and boys play sports each day.

• \(H_{0}: \mu_{g} = \mu_{b}\)  
• \(H_{0}: \mu_{g} - \mu_{b} = 0\)
• \(H_{a}: \mu_{g} \neq \mu_{b}\)  
• \(H_{a}: \mu_{g} - \mu_{b} \neq 0\)

The words "the same" tell you \(H_{0}\) has an "=". Since there are no other words to indicate \(H_{a}\), assume it says "is different." This is a two-tailed test.

Distribution for the test: Use \(t_{df}\) where \(df\) is calculated using the \(df\) formula for independent groups, two population means. Using a calculator, \(df\) is approximately 18.8462. Do not pool the variances.

Calculate the p -value using a Student's t -distribution: \(p\text{-value} = 0.0054\)

\[s_{g} = 0.866\]

\[s_{b} = 1\]

\[\bar{x}_{g} - \bar{x}_{b} = 2 - 3.2 = -1.2\]

Half the \(p\text{-value}\) is below –1.2 and half is above 1.2.

Make a decision: Since \(\alpha > p\text{-value}\), reject \(H_{0}\). This means you reject \(\mu_{g} = \mu_{b}\). The means are different.

Press STAT . Arrow over to TESTS and press 4:2-SampTTest . Arrow over to Stats and press ENTER . Arrow down and enter 2 for the first sample mean, \(\sqrt{0.866}\) for Sx1, 9 for n1, 3.2 for the second sample mean, 1 for Sx2, and 16 for n2. Arrow down to μ1: and arrow to does not equal μ2. Press ENTER . Arrow down to Pooled: and No . Press ENTER . Arrow down to Calculate and press ENTER . The \(p\text{-value}\) is \(p = 0.0054\), the dfs are approximately 18.8462, and the test statistic is -3.14. Do the procedure again but instead of Calculate do Draw.

Conclusion: At the 5% level of significance, the sample data show there is sufficient evidence to conclude that the mean number of hours that girls and boys aged seven to 11 play sports per day is different (mean number of hours boys aged seven to 11 play sports per day is greater than the mean number of hours played by girls OR the mean number of hours girls aged seven to 11 play sports per day is greater than the mean number of hours played by boys).

Exercise \(\PageIndex{1}\)

Two samples are shown in Table. Both have normal distributions. The means for the two populations are thought to be the same. Is there a difference in the means? Test at the 5% level of significance.

The \(p\text{-value}\) is \(0.4125\), which is much higher than 0.05, so we decline to reject the null hypothesis. There is not sufficient evidence to conclude that the means of the two populations are not the same.

When the sum of the sample sizes is larger than \(30 (n_{1} + n_{2} > 30)\) you can use the normal distribution to approximate the Student's \(t\).

Example \(\PageIndex{2}\)

A study is done by a community group in two neighboring colleges to determine which one graduates students with more math classes. College A samples 11 graduates. Their average is four math classes with a standard deviation of 1.5 math classes. College B samples nine graduates. Their average is 3.5 math classes with a standard deviation of one math class. The community group believes that a student who graduates from college A has taken more math classes, on the average. Both populations have a normal distribution. Test at a 1% significance level. Answer the following questions.

• Is this a test of two means or two proportions?
• Are the populations standard deviations known or unknown?
• Which distribution do you use to perform the test?
• What is the random variable?
• What are the null and alternate hypotheses? Write the null and alternate hypotheses in words and in symbols.
• Is this test right-, left-, or two-tailed?
• What is the \(p\text{-value}\)?
• Do you reject or not reject the null hypothesis?
• Student's t
• \(\bar{X}_{A} - \bar{X}_{B}\)
• \(H_{0}: \mu_{A} \leq \mu_{B}\) and \(H_{a}: \mu_{A} > \mu_{B}\)

• h. Do not reject.
• i. At the 1% level of significance, from the sample data, there is not sufficient evidence to conclude that a student who graduates from college A has taken more math classes, on the average, than a student who graduates from college B.

Exercise \(\PageIndex{2}\)

A study is done to determine if Company A retains its workers longer than Company B. Company A samples 15 workers, and their average time with the company is five years with a standard deviation of 1.2. Company B samples 20 workers, and their average time with the company is 4.5 years with a standard deviation of 0.8. The populations are normally distributed.

• Are the population standard deviations known?
• Conduct an appropriate hypothesis test. At the 5% significance level, what is your conclusion?
• They are unknown.
• The \(p\text{-value} = 0.0878\). At the 5% level of significance, there is insufficient evidence to conclude that the workers of Company A stay longer with the company.

Example \(\PageIndex{3}\)

A professor at a large community college wanted to determine whether there is a difference in the means of final exam scores between students who took his statistics course online and the students who took his face-to-face statistics class. He believed that the mean of the final exam scores for the online class would be lower than that of the face-to-face class. Was the professor correct? The randomly selected 30 final exam scores from each group are listed in Table \(\PageIndex{3}\) and Table \(\PageIndex{4}\).

Is the mean of the Final Exam scores of the online class lower than the mean of the Final Exam scores of the face-to-face class? Test at a 5% significance level. Answer the following questions:

• Are the population standard deviations known or unknown?
• What are the null and alternative hypotheses? Write the null and alternative hypotheses in words and in symbols.
• Is this test right, left, or two tailed?
• At the ___ level of significance, from the sample data, there ______ (is/is not) sufficient evidence to conclude that ______.

(See the conclusion in Example, and write yours in a similar fashion)

Be careful not to mix up the information for Group 1 and Group 2!

• Student's \(t\)
• \(\bar{X}_{1} - \bar{X}_{2}\)
• \(H_{0}: \mu_{1} = \mu_{2}\) Null hypothesis: the means of the final exam scores are equal for the online and face-to-face statistics classes.
• \(H_{a}: \mu_{1} < \mu_{2}\) Alternative hypothesis: the mean of the final exam scores of the online class is less than the mean of the final exam scores of the face-to-face class.
• left-tailed

Figure \(\PageIndex{3}\).

• Reject the null hypothesis

At the 5% level of significance, from the sample data, there is (is/is not) sufficient evidence to conclude that the mean of the final exam scores for the online class is less than the mean of final exam scores of the face-to-face class.

First put the data for each group into two lists (such as L1 and L2). Press STAT. Arrow over to TESTS and press 4:2SampTTest. Make sure Data is highlighted and press ENTER. Arrow down and enter L1 for the first list and L2 for the second list. Arrow down to \(\mu_{1}\): and arrow to \(\neq \mu_{1}\) (does not equal). Press ENTER. Arrow down to Pooled: No. Press ENTER. Arrow down to Calculate and press ENTER.

Cohen's Standards for Small, Medium, and Large Effect Sizes

Cohen's \(d\) is a measure of effect size based on the differences between two means. Cohen’s \(d\), named for United States statistician Jacob Cohen, measures the relative strength of the differences between the means of two populations based on sample data. The calculated value of effect size is then compared to Cohen’s standards of small, medium, and large effect sizes.

Cohen's \(d\) is the measure of the difference between two means divided by the pooled standard deviation: \(d = \dfrac{\bar{x}_{2}-\bar{x}_{2}}{s_{\text{pooled}}}\) where \(s_{pooled} = \sqrt{\dfrac{(n_{1}-1)s^{2}_{1} + (n_{2}-1)s^{2}_{2}}{n_{1}+n_{2}-2}}\)

Example \(\PageIndex{4}\)

Calculate Cohen’s d for Example. Is the size of the effect small, medium, or large? Explain what the size of the effect means for this problem.

\(\mu_{1} = 4 s_{1} = 1.5 n_{1} = 11\)

\(\mu_{2} = 3.5 s_{2} = 1 n_{2} = 9\)

\(d = 0.384\)

The effect is small because 0.384 is between Cohen’s value of 0.2 for small effect size and 0.5 for medium effect size. The size of the differences of the means for the two colleges is small indicating that there is not a significant difference between them.

Example \(\PageIndex{5}\)

Calculate Cohen’s \(d\) for Example. Is the size of the effect small, medium or large? Explain what the size of the effect means for this problem.

\(d = 0.834\); Large, because 0.834 is greater than Cohen’s 0.8 for a large effect size. The size of the differences between the means of the Final Exam scores of online students and students in a face-to-face class is large indicating a significant difference.

Example 10.2.6

Weighted alpha is a measure of risk-adjusted performance of stocks over a period of a year. A high positive weighted alpha signifies a stock whose price has risen while a small positive weighted alpha indicates an unchanged stock price during the time period. Weighted alpha is used to identify companies with strong upward or downward trends. The weighted alpha for the top 30 stocks of banks in the northeast and in the west as identified by Nasdaq on May 24, 2013 are listed in Table and Table, respectively.

Is there a difference in the weighted alpha of the top 30 stocks of banks in the northeast and in the west? Test at a 5% significance level. Answer the following questions:

• Calculate Cohen’s d and interpret it.
• Student’s-t
• \(H_{0}: \mu_{1} = \mu_{2}\) Null hypothesis: the means of the weighted alphas are equal.
• \(H_{a}: \mu_{1} \neq \mu_{2}\) Alternative hypothesis : the means of the weighted alphas are not equal.
• \(p\text{-value} = 0.8787\)
• Do not reject the null hypothesis

Figure \(\PageIndex{4}\).

• \(d = 0.040\), Very small, because 0.040 is less than Cohen’s value of 0.2 for small effect size. The size of the difference of the means of the weighted alphas for the two regions of banks is small indicating that there is not a significant difference between their trends in stocks.
• Data from Graduating Engineer + Computer Careers. Available online at www.graduatingengineer.com
• Data from Microsoft Bookshelf .
• Data from the United States Senate website, available online at www.Senate.gov (accessed June 17, 2013).
• “List of current United States Senators by Age.” Wikipedia. Available online at en.Wikipedia.org/wiki/List_of...enators_by_age (accessed June 17, 2013).
• “Sectoring by Industry Groups.” Nasdaq. Available online at www.nasdaq.com/markets/barcha...&base=industry (accessed June 17, 2013).
• “Strip Clubs: Where Prostitution and Trafficking Happen.” Prostitution Research and Education, 2013. Available online at www.prostitutionresearch.com/ProsViolPosttrauStress.html (accessed June 17, 2013).
• “World Series History.” Baseball-Almanac, 2013. Available online at http://www.baseball-almanac.com/ws/wsmenu.shtml (accessed June 17, 2013).

Two population means from independent samples where the population standard deviations are not known

• Random Variable: \(\bar{X}_{1} - \bar{X}_{2} =\) the difference of the sampling means
• Distribution: Student's t -distribution with degrees of freedom (variances not pooled)

Formula Review

Standard error: \[SE = \sqrt{\dfrac{(s_{1}^{2})}{n_{1}} + \dfrac{(s_{2}^{2})}{n_{2}}}\]

Test statistic ( t -score): \[t = \dfrac{(\bar{x}_{1}-\bar{x}_{2}) - (\mu_{1}-\mu_{2})}{\sqrt{\dfrac{(s_{1})^{2}}{n_{1}} + \dfrac{(s_{2})^{2}}{n_{2}}}}\]

Degrees of freedom:

\[df = \dfrac{\left(\dfrac{(s_{1})^{2}}{n_{1}} + \dfrac{(s_{2})^{2}}{n_{2}}\right)^{2}}{\left(\dfrac{1}{n_{1} - 1}\right)\left(\dfrac{(s_{1})^{2}}{n_{1}}\right)^{2}} + \left(\dfrac{1}{n_{2} - 1}\right)\left(\dfrac{(s_{2})^{2}}{n_{2}}\right)^{2}\]

• \(s_{1}\) and \(s_{2}\) are the sample standard deviations, and n 1 and n 2 are the sample sizes.
• \(x_{1}\) and \(x_{2}\) are the sample means.

OR use the   DF to be the smallest of \(n_{1}-1\) and \(n_{2}-1\)

Cohen’s \(d\) is the measure of effect size:

\[d = \dfrac{\bar{x}_{1} - \bar{x}_{2}}{s_{\text{pooled}}}\]

\[s_{\text{pooled}} = \sqrt{\dfrac{(n_{1} - 1)s^{2}_{1} + (n_{2} - 1)s^{2}_{2}}{n_{1} + n_{2} - 2}}\]

• The domain of the random variable (RV) is not necessarily a numerical set; the domain may be expressed in words; for example, if \(X =\) hair color, then the domain is {black, blond, gray, green, orange}.
• We can tell what specific value x of the random variable \(X\) takes only after performing the experiment.

• Calculators
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• Which Statistics Test?

T-Test Calculator for 2 Independent Means

This simple t -test calculator, provides full details of the t-test calculation, including sample mean, sum of squares and standard deviation.

Further Information

A t -test is used when you're looking at a numerical variable - for example, height - and then comparing the averages of two separate populations or groups (e.g., males and females).

Requirements

• Two independent samples
• Data should be normally distributed
• The two samples should have the same variance

Null Hypothesis

H0: u1 - u2 = 0, where u1 is the mean of first population and u2 the mean of the second.

As above, the null hypothesis tends to be that there is no difference between the means of the two populations; or, more formally, that the difference is zero (so, for example, that there is no difference between the average heights of two populations of males and females).

Statistics Made Easy

Two Sample Z-Test: Definition, Formula, and Example

A  two sample z-test is used to test whether two population means are equal.

This test assumes that the standard deviation of each population is known.

This tutorial explains the following:

• The formula to perform a two sample z-test.
• The assumptions of a two sample z-test.
• An example of how to perform a two sample z-test.

Let’s jump in!

Two Sample Z-Test: Formula

A two sample z-test uses the following null and alternative hypotheses:

• H 0 :  μ 1 = μ 2 (the two population means are equal)
• H A :  μ 1 ≠ μ 2 (the two population means are not equal)

We use the following formula to calculate the z test statistic:

• z = ( x 1 – x 2 ) / √ σ 1 2 /n 1 + σ 2 2 /n 2 )
• x 1 , x 2 : sample means
• σ 1 , σ 2 : population standard deviations
• n 1 , n 2 : sample sizes

If the p-value that corresponds to the z test statistic is less than your chosen significance level (common choices are 0.10, 0.05, and 0.01) then you can reject the null hypothesis .

Two Sample Z-Test: Assumptions

For the results of a two sample z-test to be valid, the following assumptions should be met:

• The data from each population are continuous (not discrete).
• Each sample is a simple random sample from the population of interest.
• The data in each population is approximately normally distributed .
• The population standard deviations are known.

Two Sample Z-Test : Example

Suppose the IQ levels among individuals in two different cities are known to be normally distributed each with population standard deviations of 15.

A scientist wants to know if the mean IQ level between individuals in city A and city B are different, so she selects a simple random sample of  20 individuals from each city and records their IQ levels.

To test this, she will perform a two sample z-test at significance level α = 0.05 using the following steps:

Step 1: Gather the sample data.

Suppose she collects two simple random samples with the following information:

•   x 1  (sample 1 mean IQ) = 100.65
• n 1 (sample 1 size) = 20
• x 2 (sample 2 mean IQ) = 108.8
• n 2 (sample 2 size) = 20

Step 2: Define the hypotheses.

She will perform the two sample z-test with the following hypotheses:

Step 3: Calculate the z test statistic.

The z test statistic is calculated as:

• z = (100.65-108.8) / √ 15 2 /20 + 15 2 /20)

Step 4: Calculate the p-value of the z test statistic.

According to the Z Score to P Value Calculator , the two-tailed p-value associated with z = -1.718 is 0.0858 .

Step 5: Draw a conclusion.

Since the p-value (0.0858) is not less than the significance level (.05), the scientist will fail to reject the null hypothesis.

There is not sufficient evidence to say that the mean IQ level is different between the two populations.

Note:  You can also perform this entire two sample z-test by using the Two Sample Z-Test Calculator .

Additional Resources

The following tutorials explain how to perform a two sample z-test using different statistical software:

How to Perform Z-Tests in Excel How to Perform Z-Tests in R How to Perform Z-Tests in Python

Featured Posts

Hey there. My name is Zach Bobbitt. I have a Masters of Science degree in Applied Statistics and I’ve worked on machine learning algorithms for professional businesses in both healthcare and retail. I’m passionate about statistics, machine learning, and data visualization and I created Statology to be a resource for both students and teachers alike.  My goal with this site is to help you learn statistics through using simple terms, plenty of real-world examples, and helpful illustrations.

One Reply to “Two Sample Z-Test: Definition, Formula, and Example”

I’m a 200 Level Statistics Student. And this has really helped me.

God bless you Soo much.

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32: Two Independent Samples With Statistics Calculator

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Two Independent Samples with statistics Calculator

Enter in the statistics, the tail type and the confidence level and hit Calculate and the test statistic, t, the p-value, p, the confidence interval's lower bound, LB, and the upper bound, UB will be shown.  Be sure to enter the confidence level as a decimal, e.g., 95% has a CL of 0.95.

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