what does hypothesis mean biology

Hypothesis n., plural: hypotheses [/haɪˈpɑːθəsɪs/] Definition: Testable scientific prediction

Table of Contents

What Is Hypothesis?

A scientific hypothesis is a foundational element of the scientific method . It’s a testable statement proposing a potential explanation for natural phenomena. The term hypothesis means “little theory” . A hypothesis is a short statement that can be tested and gives a possible reason for a phenomenon or a possible link between two variables . In the setting of scientific research, a hypothesis is a tentative explanation or statement that can be proven wrong and is used to guide experiments and empirical research.

It is an important part of the scientific method because it gives a basis for planning tests, gathering data, and judging evidence to see if it is true and could help us understand how natural things work. Several hypotheses can be tested in the real world, and the results of careful and systematic observation and analysis can be used to support, reject, or improve them.

Researchers and scientists often use the word hypothesis to refer to this educated guess . These hypotheses are firmly established based on scientific principles and the rigorous testing of new technology and experiments .

For example, in astrophysics, the Big Bang Theory is a working hypothesis that explains the origins of the universe and considers it as a natural phenomenon. It is among the most prominent scientific hypotheses in the field.

“The scientific method: steps, terms, and examples” by Scishow:

Biology definition: A hypothesis  is a supposition or tentative explanation for (a group of) phenomena, (a set of) facts, or a scientific inquiry that may be tested, verified or answered by further investigation or methodological experiment. It is like a scientific guess . It’s an idea or prediction that scientists make before they do experiments. They use it to guess what might happen and then test it to see if they were right. It’s like a smart guess that helps them learn new things. A scientific hypothesis that has been verified through scientific experiment and research may well be considered a scientific theory .

Etymology: The word “hypothesis” comes from the Greek word “hupothesis,” which means “a basis” or “a supposition.” It combines “hupo” (under) and “thesis” (placing). Synonym: proposition; assumption; conjecture; postulate Compare:   theory See also: null hypothesis

Characteristics Of Hypothesis

A useful hypothesis must have the following qualities:

It should never be written as a question.
You should be able to test it in the real world to see if it’s right or wrong.
It needs to be clear and exact.
It should list the factors that will be used to figure out the relationship.
It should only talk about one thing. You can make a theory in either a descriptive or form of relationship.
It shouldn’t go against any natural rule that everyone knows is true. Verification will be done well with the tools and methods that are available.
It should be written in as simple a way as possible so that everyone can understand it.
It must explain what happened to make an answer necessary.
It should be testable in a fair amount of time.
It shouldn’t say different things.

Sources Of Hypothesis

Sources of hypothesis are:

Patterns of similarity between the phenomenon under investigation and existing hypotheses.
Insights derived from prior research, concurrent observations, and insights from opposing perspectives.
The formulations are derived from accepted scientific theories and proposed by researchers.
In research, it’s essential to consider hypothesis as different subject areas may require various hypotheses (plural form of hypothesis). Researchers also establish a significance level to determine the strength of evidence supporting a hypothesis.
Individual cognitive processes also contribute to the formation of hypotheses.

One hypothesis is a tentative explanation for an observation or phenomenon. It is based on prior knowledge and understanding of the world, and it can be tested by gathering and analyzing data. Observed facts are the data that are collected to test a hypothesis. They can support or refute the hypothesis.

For example, the hypothesis that “eating more fruits and vegetables will improve your health” can be tested by gathering data on the health of people who eat different amounts of fruits and vegetables. If the people who eat more fruits and vegetables are healthier than those who eat less fruits and vegetables, then the hypothesis is supported.

Hypotheses are essential for scientific inquiry. They help scientists to focus their research, to design experiments, and to interpret their results. They are also essential for the development of scientific theories.

Types Of Hypothesis

In research, you typically encounter two types of hypothesis: the alternative hypothesis (which proposes a relationship between variables) and the null hypothesis (which suggests no relationship).

Simple Hypothesis

It illustrates the association between one dependent variable and one independent variable. For instance, if you consume more vegetables, you will lose weight more quickly. Here, increasing vegetable consumption is the independent variable, while weight loss is the dependent variable.

Complex Hypothesis

It exhibits the relationship between at least two dependent variables and at least two independent variables. Eating more vegetables and fruits results in weight loss, radiant skin, and a decreased risk of numerous diseases, including heart disease.

Directional Hypothesis

It shows that a researcher wants to reach a certain goal. The way the factors are related can also tell us about their nature. For example, four-year-old children who eat well over a time of five years have a higher IQ than children who don’t eat well. This shows what happened and how it happened.

Non-directional Hypothesis

When there is no theory involved, it is used. It is a statement that there is a connection between two variables, but it doesn’t say what that relationship is or which way it goes.

Null Hypothesis

It says something that goes against the theory. It’s a statement that says something is not true, and there is no link between the independent and dependent factors. “H 0 ” represents the null hypothesis.

Associative and Causal Hypothesis

When a change in one variable causes a change in the other variable, this is called the associative hypothesis . The causal hypothesis, on the other hand, says that there is a cause-and-effect relationship between two or more factors.

Examples Of Hypothesis

Examples of simple hypotheses:

Students who consume breakfast before taking a math test will have a better overall performance than students who do not consume breakfast.
Students who experience test anxiety before an English examination will get lower scores than students who do not experience test anxiety.
Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone, is a statement that suggests that drivers who talk on the phone while driving are more likely to make mistakes.

Examples of a complex hypothesis:

Individuals who consume a lot of sugar and don’t get much exercise are at an increased risk of developing depression.
Younger people who are routinely exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces, according to a new study.
Increased levels of air pollution led to higher rates of respiratory illnesses, which in turn resulted in increased costs for healthcare for the affected communities.

Examples of Directional Hypothesis:

The crop yield will go up a lot if the amount of fertilizer is increased.
Patients who have surgery and are exposed to more stress will need more time to get better.
Increasing the frequency of brand advertising on social media will lead to a significant increase in brand awareness among the target audience.

Examples of Non-Directional Hypothesis (or Two-Tailed Hypothesis):

The test scores of two groups of students are very different from each other.
There is a link between gender and being happy at work.
There is a correlation between the amount of caffeine an individual consumes and the speed with which they react.

Examples of a null hypothesis:

Children who receive a new reading intervention will have scores that are different than students who do not receive the intervention.
The results of a memory recall test will not reveal any significant gap in performance between children and adults.
There is not a significant relationship between the number of hours spent playing video games and academic performance.

Examples of Associative Hypothesis:

There is a link between how many hours you spend studying and how well you do in school.
Drinking sugary drinks is bad for your health as a whole.
There is an association between socioeconomic status and access to quality healthcare services in urban neighborhoods.

Functions Of Hypothesis

The research issue can be understood better with the help of a hypothesis, which is why developing one is crucial. The following are some of the specific roles that a hypothesis plays: (Rashid, Apr 20, 2022)

A hypothesis gives a study a point of concentration. It enlightens us as to the specific characteristics of a study subject we need to look into.
It instructs us on what data to acquire as well as what data we should not collect, giving the study a focal point .
The development of a hypothesis improves objectivity since it enables the establishment of a focal point.
A hypothesis makes it possible for us to contribute to the development of the theory. Because of this, we are in a position to definitively determine what is true and what is untrue .

How will Hypothesis help in the Scientific Method?

The scientific method begins with observation and inquiry about the natural world when formulating research questions. Researchers can refine their observations and queries into specific, testable research questions with the aid of hypothesis. They provide an investigation with a focused starting point.
Hypothesis generate specific predictions regarding the expected outcomes of experiments or observations. These forecasts are founded on the researcher’s current knowledge of the subject. They elucidate what researchers anticipate observing if the hypothesis is true.
Hypothesis direct the design of experiments and data collection techniques. Researchers can use them to determine which variables to measure or manipulate, which data to obtain, and how to conduct systematic and controlled research.
Following the formulation of a hypothesis and the design of an experiment, researchers collect data through observation, measurement, or experimentation. The collected data is used to verify the hypothesis’s predictions.
Hypothesis establish the criteria for evaluating experiment results. The observed data are compared to the predictions generated by the hypothesis. This analysis helps determine whether empirical evidence supports or refutes the hypothesis.
The results of experiments or observations are used to derive conclusions regarding the hypothesis. If the data support the predictions, then the hypothesis is supported. If this is not the case, the hypothesis may be revised or rejected, leading to the formulation of new queries and hypothesis.
The scientific approach is iterative, resulting in new hypothesis and research issues from previous trials. This cycle of hypothesis generation, testing, and refining drives scientific progress.

Importance Of Hypothesis

Hypothesis are testable statements that enable scientists to determine if their predictions are accurate. This assessment is essential to the scientific method, which is based on empirical evidence.
Hypothesis serve as the foundation for designing experiments or data collection techniques. They can be used by researchers to develop protocols and procedures that will produce meaningful results.
Hypothesis hold scientists accountable for their assertions. They establish expectations for what the research should reveal and enable others to assess the validity of the findings.
Hypothesis aid in identifying the most important variables of a study. The variables can then be measured, manipulated, or analyzed to determine their relationships.
Hypothesis assist researchers in allocating their resources efficiently. They ensure that time, money, and effort are spent investigating specific concerns, as opposed to exploring random concepts.
Testing hypothesis contribute to the scientific body of knowledge. Whether or not a hypothesis is supported, the results contribute to our understanding of a phenomenon.
Hypothesis can result in the creation of theories. When supported by substantive evidence, hypothesis can serve as the foundation for larger theoretical frameworks that explain complex phenomena.
Beyond scientific research, hypothesis play a role in the solution of problems in a variety of domains. They enable professionals to make educated assumptions about the causes of problems and to devise solutions.

Research Hypotheses: Did you know that a hypothesis refers to an educated guess or prediction about the outcome of a research study?

It’s like a roadmap guiding researchers towards their destination of knowledge. Just like a compass points north, a well-crafted hypothesis points the way to valuable discoveries in the world of science and inquiry.

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Hypothesis basics

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Types of hypotheses
Hypothesis versus theory

Additional resources

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A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research.

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

Elementary-age students study alternative energy using homemade windmills during public school science class.

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (BCcampus, 2015).

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley .

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts."

Read more about writing a hypothesis, from the American Medical Writers Association.
Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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Definition of hypothesis

Did you know.

The Difference Between Hypothesis and Theory

A hypothesis is an assumption, an idea that is proposed for the sake of argument so that it can be tested to see if it might be true.

In the scientific method, the hypothesis is constructed before any applicable research has been done, apart from a basic background review. You ask a question, read up on what has been studied before, and then form a hypothesis.

A hypothesis is usually tentative; it's an assumption or suggestion made strictly for the objective of being tested.

A theory , in contrast, is a principle that has been formed as an attempt to explain things that have already been substantiated by data. It is used in the names of a number of principles accepted in the scientific community, such as the Big Bang Theory . Because of the rigors of experimentation and control, it is understood to be more likely to be true than a hypothesis is.

In non-scientific use, however, hypothesis and theory are often used interchangeably to mean simply an idea, speculation, or hunch, with theory being the more common choice.

Since this casual use does away with the distinctions upheld by the scientific community, hypothesis and theory are prone to being wrongly interpreted even when they are encountered in scientific contexts—or at least, contexts that allude to scientific study without making the critical distinction that scientists employ when weighing hypotheses and theories.

The most common occurrence is when theory is interpreted—and sometimes even gleefully seized upon—to mean something having less truth value than other scientific principles. (The word law applies to principles so firmly established that they are almost never questioned, such as the law of gravity.)

This mistake is one of projection: since we use theory in general to mean something lightly speculated, then it's implied that scientists must be talking about the same level of uncertainty when they use theory to refer to their well-tested and reasoned principles.

The distinction has come to the forefront particularly on occasions when the content of science curricula in schools has been challenged—notably, when a school board in Georgia put stickers on textbooks stating that evolution was "a theory, not a fact, regarding the origin of living things." As Kenneth R. Miller, a cell biologist at Brown University, has said , a theory "doesn’t mean a hunch or a guess. A theory is a system of explanations that ties together a whole bunch of facts. It not only explains those facts, but predicts what you ought to find from other observations and experiments.”

While theories are never completely infallible, they form the basis of scientific reasoning because, as Miller said "to the best of our ability, we’ve tested them, and they’ve held up."

proposition
supposition

hypothesis , theory , law mean a formula derived by inference from scientific data that explains a principle operating in nature.

hypothesis implies insufficient evidence to provide more than a tentative explanation.

theory implies a greater range of evidence and greater likelihood of truth.

law implies a statement of order and relation in nature that has been found to be invariable under the same conditions.

Examples of hypothesis in a Sentence

These examples are programmatically compiled from various online sources to illustrate current usage of the word 'hypothesis.' Any opinions expressed in the examples do not represent those of Merriam-Webster or its editors. Send us feedback about these examples.

Word History

Greek, from hypotithenai to put under, suppose, from hypo- + tithenai to put — more at do

1641, in the meaning defined at sense 1a

Phrases Containing hypothesis

nebular hypothesis
planetesimal hypothesis
null hypothesis
counter - hypothesis
Whorfian hypothesis

Articles Related to hypothesis

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This is the Difference Between a Hypothesis and a Theory

In scientific reasoning, they're two completely different things

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Cite this Entry

“Hypothesis.” Merriam-Webster.com Dictionary , Merriam-Webster, https://www.merriam-webster.com/dictionary/hypothesis. Accessed 28 Mar. 2024.

Kids Definition

Kids definition of hypothesis, medical definition, medical definition of hypothesis, more from merriam-webster on hypothesis.

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Biology library

Course: biology library > unit 1.

The scientific method

Controlled experiments

The scientific method and experimental design

Introduction

How are hypotheses tested.

One pot of seeds gets watered every afternoon.
The other pot of seeds doesn't get any water at all.

Control and experimental groups

Independent and dependent variables, independent variables, dependent variables, variability and repetition, controlled experiment case study: co 2 ‍ and coral bleaching.

What your control and experimental groups would be
What your independent and dependent variables would be
What results you would predict in each group

Experimental setup

Some corals were grown in tanks of normal seawater, which is not very acidic ( pH ‍ around 8.2 ‍ ). The corals in these tanks served as the control group .
Other corals were grown in tanks of seawater that were more acidic than usual due to addition of CO 2 ‍ . One set of tanks was medium-acidity ( pH ‍ about 7.9 ‍ ), while another set was high-acidity ( pH ‍ about 7.65 ‍ ). Both the medium-acidity and high-acidity groups were experimental groups .
In this experiment, the independent variable was the acidity ( pH ‍ ) of the seawater. The dependent variable was the degree of bleaching of the corals.
The researchers used a large sample size and repeated their experiment. Each tank held 5 ‍ fragments of coral, and there were 5 ‍ identical tanks for each group (control, medium-acidity, and high-acidity). Experimental setup to test effects of water acidity on coral bleaching. Control group: Coral fragments are placed in a tank of normal seawater (pH 8.2). Experimental group 1: Coral fragments are placed in a tank of slightly acidified seawater (pH 7.9). Experimental group 2: Coral fragments are placed in a tank of more strongly acidified seawater (pH 7.65). The water acidity is the independent variable. 8 weeks are allowed to pass for each of the tanks... Control group: Corals are about 10% bleached on average. Experimental group 1 (medium acidity): Corals are about 20% bleached on average. Experimental group 2 (higher acidity): Corals are about 40% bleached on average. Degree of coral bleaching is the dependent variable. Note: None of these tanks was "acidic" on an absolute scale. That is, the pH ‍ values were all above the neutral pH ‍ of 7.0 ‍ . However, the two groups of experimental tanks were moderately and highly acidic to the corals , that is, relative to their natural habitat of plain seawater.

Analyzing the results

Non-experimental hypothesis tests, case study: coral bleaching and temperature, attribution:, works cited:.

Hoegh-Guldberg, O. (1999). Climate change, coral bleaching, and the future of the world's coral reefs. Mar. Freshwater Res. , 50 , 839-866. Retrieved from www.reef.edu.au/climate/Hoegh-Guldberg%201999.pdf.
Anthony, K. R. N., Kline, D. I., Diaz-Pulido, G., Dove, S., and Hoegh-Guldberg, O. (2008). Ocean acidification causes bleaching and productivity loss in coral reef builders. PNAS , 105 (45), 17442-17446. http://dx.doi.org/10.1073/pnas.0804478105 .
University of California Museum of Paleontology. (2016). Misconceptions about science. In Understanding science . Retrieved from http://undsci.berkeley.edu/teaching/misconceptions.php .
Hoegh-Guldberg, O. and Smith, G. J. (1989). The effect of sudden changes in temperature, light and salinity on the density and export of zooxanthellae from the reef corals Stylophora pistillata (Esper, 1797) and Seriatopora hystrix (Dana, 1846). J. Exp. Mar. Biol. Ecol. , 129 , 279-303. Retrieved from http://www.reef.edu.au/ohg/res-pic/HG%20papers/HG%20and%20Smith%201989%20BLEACH.pdf .

Additional references:

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Knowledge Base

Methodology

How to Write a Strong Hypothesis | Steps & Examples

How to Write a Strong Hypothesis | Steps & Examples

Published on May 6, 2022 by Shona McCombes . Revised on November 20, 2023.

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection .

Example: Hypothesis

Daily apple consumption leads to fewer doctor’s visits.

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, other interesting articles, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more types of variables .

An independent variable is something the researcher changes or controls.
A dependent variable is something the researcher observes and measures.

If there are any control variables , extraneous variables , or confounding variables , be sure to jot those down as you go to minimize the chances that research bias will affect your results.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1. Ask a question

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2. Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to ensure that you’re embarking on a relevant topic . This can also help you identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalize more complex constructs.

Step 3. Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

4. Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

The relevant variables
The specific group being studied
The predicted outcome of the experiment or analysis

5. Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if…then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis . The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

H 0 : The number of lectures attended by first-year students has no effect on their final exam scores.
H 1 : The number of lectures attended by first-year students has a positive effect on their final exam scores.

If you want to know more about the research process , methodology , research bias , or statistics , make sure to check out some of our other articles with explanations and examples.

Sampling methods
Simple random sampling
Stratified sampling
Cluster sampling
Likert scales
Reproducibility

Statistics

Null hypothesis
Statistical power
Probability distribution
Effect size
Poisson distribution

Research bias

Optimism bias
Cognitive bias
Implicit bias
Hawthorne effect
Anchoring bias
Explicit bias

Prevent plagiarism. Run a free check.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

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1.2 The Process of Science

Learning objectives.

Identify the shared characteristics of the natural sciences
Understand the process of scientific inquiry
Compare inductive reasoning with deductive reasoning
Describe the goals of basic science and applied science

Like geology, physics, and chemistry, biology is a science that gathers knowledge about the natural world. Specifically, biology is the study of life. The discoveries of biology are made by a community of researchers who work individually and together using agreed-on methods. In this sense, biology, like all sciences is a social enterprise like politics or the arts. The methods of science include careful observation, record keeping, logical and mathematical reasoning, experimentation, and submitting conclusions to the scrutiny of others. Science also requires considerable imagination and creativity; a well-designed experiment is commonly described as elegant, or beautiful. Like politics, science has considerable practical implications and some science is dedicated to practical applications, such as the prevention of disease (see Figure 1.15 ). Other science proceeds largely motivated by curiosity. Whatever its goal, there is no doubt that science, including biology, has transformed human existence and will continue to do so.

The Nature of Science

Biology is a science, but what exactly is science? What does the study of biology share with other scientific disciplines? Science (from the Latin scientia, meaning "knowledge") can be defined as knowledge about the natural world.

Science is a very specific way of learning, or knowing, about the world. The history of the past 500 years demonstrates that science is a very powerful way of knowing about the world; it is largely responsible for the technological revolutions that have taken place during this time. There are however, areas of knowledge and human experience that the methods of science cannot be applied to. These include such things as answering purely moral questions, aesthetic questions, or what can be generally categorized as spiritual questions. Science cannot investigate these areas because they are outside the realm of material phenomena, the phenomena of matter and energy, and cannot be observed and measured.

The scientific method is a method of research with defined steps that include experiments and careful observation. The steps of the scientific method will be examined in detail later, but one of the most important aspects of this method is the testing of hypotheses. A hypothesis is a suggested explanation for an event, which can be tested. Hypotheses, or tentative explanations, are generally produced within the context of a scientific theory . A generally accepted scientific theory is thoroughly tested and confirmed explanation for a set of observations or phenomena. Scientific theory is the foundation of scientific knowledge. In addition, in many scientific disciplines (less so in biology) there are scientific laws , often expressed in mathematical formulas, which describe how elements of nature will behave under certain specific conditions. There is not an evolution of hypotheses through theories to laws as if they represented some increase in certainty about the world. Hypotheses are the day-to-day material that scientists work with and they are developed within the context of theories. Laws are concise descriptions of parts of the world that are amenable to formulaic or mathematical description.

Natural Sciences

What would you expect to see in a museum of natural sciences? Frogs? Plants? Dinosaur skeletons? Exhibits about how the brain functions? A planetarium? Gems and minerals? Or maybe all of the above? Science includes such diverse fields as astronomy, biology, computer sciences, geology, logic, physics, chemistry, and mathematics ( Figure 1.16 ). However, those fields of science related to the physical world and its phenomena and processes are considered natural sciences . Thus, a museum of natural sciences might contain any of the items listed above.

There is no complete agreement when it comes to defining what the natural sciences include. For some experts, the natural sciences are astronomy, biology, chemistry, earth science, and physics. Other scholars choose to divide natural sciences into life sciences , which study living things and include biology, and physical sciences , which study nonliving matter and include astronomy, physics, and chemistry. Some disciplines such as biophysics and biochemistry build on two sciences and are interdisciplinary.

Scientific Inquiry

One thing is common to all forms of science: an ultimate goal “to know.” Curiosity and inquiry are the driving forces for the development of science. Scientists seek to understand the world and the way it operates. Two methods of logical thinking are used: inductive reasoning and deductive reasoning.

Inductive reasoning is a form of logical thinking that uses related observations to arrive at a general conclusion. This type of reasoning is common in descriptive science. A life scientist such as a biologist makes observations and records them. These data can be qualitative (descriptive) or quantitative (consisting of numbers), and the raw data can be supplemented with drawings, pictures, photos, or videos. From many observations, the scientist can infer conclusions (inductions) based on evidence. Inductive reasoning involves formulating generalizations inferred from careful observation and the analysis of a large amount of data. Brain studies often work this way. Many brains are observed while people are doing a task. The part of the brain that lights up, indicating activity, is then demonstrated to be the part controlling the response to that task.

Deductive reasoning or deduction is the type of logic used in hypothesis-based science. In deductive reasoning, the pattern of thinking moves in the opposite direction as compared to inductive reasoning. Deductive reasoning is a form of logical thinking that uses a general principle or law to predict specific results. From those general principles, a scientist can deduce and predict the specific results that would be valid as long as the general principles are valid. For example, a prediction would be that if the climate is becoming warmer in a region, the distribution of plants and animals should change. Comparisons have been made between distributions in the past and the present, and the many changes that have been found are consistent with a warming climate. Finding the change in distribution is evidence that the climate change conclusion is a valid one.

Both types of logical thinking are related to the two main pathways of scientific study: descriptive science and hypothesis-based science. Descriptive (or discovery) science aims to observe, explore, and discover, while hypothesis-based science begins with a specific question or problem and a potential answer or solution that can be tested. The boundary between these two forms of study is often blurred, because most scientific endeavors combine both approaches. Observations lead to questions, questions lead to forming a hypothesis as a possible answer to those questions, and then the hypothesis is tested. Thus, descriptive science and hypothesis-based science are in continuous dialogue.

Hypothesis Testing

Biologists study the living world by posing questions about it and seeking science-based responses. This approach is common to other sciences as well and is often referred to as the scientific method. The scientific method was used even in ancient times, but it was first documented by England’s Sir Francis Bacon (1561–1626) ( Figure 1.17 ), who set up inductive methods for scientific inquiry. The scientific method is not exclusively used by biologists but can be applied to almost anything as a logical problem-solving method.

The scientific process typically starts with an observation (often a problem to be solved) that leads to a question. Let’s think about a simple problem that starts with an observation and apply the scientific method to solve the problem. One Monday morning, a student arrives at class and quickly discovers that the classroom is too warm. That is an observation that also describes a problem: the classroom is too warm. The student then asks a question: “Why is the classroom so warm?”

Recall that a hypothesis is a suggested explanation that can be tested. To solve a problem, several hypotheses may be proposed. For example, one hypothesis might be, “The classroom is warm because no one turned on the air conditioning.” But there could be other responses to the question, and therefore other hypotheses may be proposed. A second hypothesis might be, “The classroom is warm because there is a power failure, and so the air conditioning doesn’t work.”

Once a hypothesis has been selected, a prediction may be made. A prediction is similar to a hypothesis but it typically has the format “If . . . then . . . .” For example, the prediction for the first hypothesis might be, “ If the student turns on the air conditioning, then the classroom will no longer be too warm.”

A hypothesis must be testable to ensure that it is valid. For example, a hypothesis that depends on what a bear thinks is not testable, because it can never be known what a bear thinks. It should also be falsifiable , meaning that it can be disproven by experimental results. An example of an unfalsifiable hypothesis is “Botticelli’s Birth of Venus is beautiful.” There is no experiment that might show this statement to be false. To test a hypothesis, a researcher will conduct one or more experiments designed to eliminate one or more of the hypotheses. This is important. A hypothesis can be disproven, or eliminated, but it can never be proven. Science does not deal in proofs like mathematics. If an experiment fails to disprove a hypothesis, then we find support for that explanation, but this is not to say that down the road a better explanation will not be found, or a more carefully designed experiment will be found to falsify the hypothesis.

Each experiment will have one or more variables and one or more controls. A variable is any part of the experiment that can vary or change during the experiment. A control is a part of the experiment that does not change. Look for the variables and controls in the example that follows. As a simple example, an experiment might be conducted to test the hypothesis that phosphate limits the growth of algae in freshwater ponds. A series of artificial ponds are filled with water and half of them are treated by adding phosphate each week, while the other half are treated by adding a salt that is known not to be used by algae. The variable here is the phosphate (or lack of phosphate), the experimental or treatment cases are the ponds with added phosphate and the control ponds are those with something inert added, such as the salt. Just adding something is also a control against the possibility that adding extra matter to the pond has an effect. If the treated ponds show lesser growth of algae, then we have found support for our hypothesis. If they do not, then we reject our hypothesis. Be aware that rejecting one hypothesis does not determine whether or not the other hypotheses can be accepted; it simply eliminates one hypothesis that is not valid ( Figure 1.18 ). Using the scientific method, the hypotheses that are inconsistent with experimental data are rejected.

In recent years a new approach of testing hypotheses has developed as a result of an exponential growth of data deposited in various databases. Using computer algorithms and statistical analyses of data in databases, a new field of so-called "data research" (also referred to as "in silico" research) provides new methods of data analyses and their interpretation. This will increase the demand for specialists in both biology and computer science, a promising career opportunity.

Visual Connection

In the example below, the scientific method is used to solve an everyday problem. Which part in the example below is the hypothesis? Which is the prediction? Based on the results of the experiment, is the hypothesis supported? If it is not supported, propose some alternative hypotheses.

My toaster doesn’t toast my bread.
Why doesn’t my toaster work?
There is something wrong with the electrical outlet.
If something is wrong with the outlet, my coffeemaker also won’t work when plugged into it.
I plug my coffeemaker into the outlet.
My coffeemaker works.

In practice, the scientific method is not as rigid and structured as it might at first appear. Sometimes an experiment leads to conclusions that favor a change in approach; often, an experiment brings entirely new scientific questions to the puzzle. Many times, science does not operate in a linear fashion; instead, scientists continually draw inferences and make generalizations, finding patterns as their research proceeds. Scientific reasoning is more complex than the scientific method alone suggests.

Basic and Applied Science

The scientific community has been debating for the last few decades about the value of different types of science. Is it valuable to pursue science for the sake of simply gaining knowledge, or does scientific knowledge only have worth if we can apply it to solving a specific problem or bettering our lives? This question focuses on the differences between two types of science: basic science and applied science.

Basic science or “pure” science seeks to expand knowledge regardless of the short-term application of that knowledge. It is not focused on developing a product or a service of immediate public or commercial value. The immediate goal of basic science is knowledge for knowledge’s sake, though this does not mean that in the end it may not result in an application.

In contrast, applied science or “technology,” aims to use science to solve real-world problems, making it possible, for example, to improve a crop yield, find a cure for a particular disease, or save animals threatened by a natural disaster. In applied science, the problem is usually defined for the researcher.

Some individuals may perceive applied science as “useful” and basic science as “useless.” A question these people might pose to a scientist advocating knowledge acquisition would be, “What for?” A careful look at the history of science, however, reveals that basic knowledge has resulted in many remarkable applications of great value. Many scientists think that a basic understanding of science is necessary before an application is developed; therefore, applied science relies on the results generated through basic science. Other scientists think that it is time to move on from basic science and instead to find solutions to actual problems. Both approaches are valid. It is true that there are problems that demand immediate attention; however, few solutions would be found without the help of the knowledge generated through basic science.

One example of how basic and applied science can work together to solve practical problems occurred after the discovery of DNA structure led to an understanding of the molecular mechanisms governing DNA replication. Strands of DNA, unique in every human, are found in our cells, where they provide the instructions necessary for life. During DNA replication, new copies of DNA are made, shortly before a cell divides to form new cells. Understanding the mechanisms of DNA replication enabled scientists to develop laboratory techniques that are now used to identify genetic diseases, pinpoint individuals who were at a crime scene, and determine paternity. Without basic science, it is unlikely that applied science could exist.

Another example of the link between basic and applied research is the Human Genome Project, a study in which each human chromosome was analyzed and mapped to determine the precise sequence of DNA subunits and the exact location of each gene. (The gene is the basic unit of heredity represented by a specific DNA segment that codes for a functional molecule.) Other organisms have also been studied as part of this project to gain a better understanding of human chromosomes. The Human Genome Project ( Figure 1.19 ) relied on basic research carried out with non-human organisms and, later, with the human genome. An important end goal eventually became using the data for applied research seeking cures for genetically related diseases.

While research efforts in both basic science and applied science are usually carefully planned, it is important to note that some discoveries are made by serendipity, that is, by means of a fortunate accident or a lucky surprise. Penicillin was discovered when biologist Alexander Fleming accidentally left a petri dish of Staphylococcus bacteria open. An unwanted mold grew, killing the bacteria. The mold turned out to be Penicillium , and a new critically important antibiotic was discovered. In a similar manner, Percy Lavon Julian was an established medicinal chemist working on a way to mass produce compounds with which to manufacture important drugs. He was focused on using soybean oil in the production of progesterone (a hormone important in the menstrual cycle and pregnancy), but it wasn't until water accidentally leaked into a large soybean oil storage tank that he found his method. Immediately recognizing the resulting substance as stigmasterol, a primary ingredient in progesterone and similar drugs, he began the process of replicating and industrializing the process in a manner that has helped millions of people. Even in the highly organized world of science, luck—when combined with an observant, curious mind focused on the types of reasoning discussed above—can lead to unexpected breakthroughs.

Reporting Scientific Work

Whether scientific research is basic science or applied science, scientists must share their findings for other researchers to expand and build upon their discoveries. Communication and collaboration within and between sub disciplines of science are key to the advancement of knowledge in science. For this reason, an important aspect of a scientist’s work is disseminating results and communicating with peers. Scientists can share results by presenting them at a scientific meeting or conference, but this approach can reach only the limited few who are present. Instead, most scientists present their results in peer-reviewed articles that are published in scientific journals. Peer-reviewed articles are scientific papers that are reviewed, usually anonymously by a scientist’s colleagues, or peers. These colleagues are qualified individuals, often experts in the same research area, who judge whether or not the scientist’s work is suitable for publication. The process of peer review helps to ensure that the research described in a scientific paper or grant proposal is original, significant, logical, and thorough. Grant proposals, which are requests for research funding, are also subject to peer review. Scientists publish their work so other scientists can reproduce their experiments under similar or different conditions to expand on the findings.

There are many journals and the popular press that do not use a peer-review system. A large number of online open-access journals, journals with articles available without cost, are now available many of which use rigorous peer-review systems, but some of which do not. Results of any studies published in these forums without peer review are not reliable and should not form the basis for other scientific work. In one exception, journals may allow a researcher to cite a personal communication from another researcher about unpublished results with the cited author’s permission.

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1 Hypothesis Testing

Biology is a science, but what exactly is science? What does the study of biology share with other scientific disciplines? Science (from the Latin scientia, meaning “knowledge”) can be defined as knowledge about the natural world.

Biologists study the living world by posing questions about it and seeking science-based responses. This approach is common to other sciences as well and is often referred to as the scientific method . The scientific process was used even in ancient times, but it was first documented by England’s Sir Francis Bacon (1561–1626) ( Figure 1 ), who set up inductive methods for scientific inquiry. The scientific method is not exclusively used by biologists but can be applied to almost anything as a logical problem solving method.

a painting of a guy wearing historical clothing

The scientific process typically starts with an observation (often a problem to be solved) that leads to a question. Science is very good at answering questions having to do with observations about the natural world, but is very bad at answering questions having to do with purely moral questions, aesthetic questions, personal opinions, or what can be generally categorized as spiritual questions. Science has cannot investigate these areas because they are outside the realm of material phenomena, the phenomena of matter and energy, and cannot be observed and measured.

Let’s think about a simple problem that starts with an observation and apply the scientific method to solve the problem. Imagine that one morning when you wake up and flip a the switch to turn on your bedside lamp, the light won’t turn on. That is an observation that also describes a problem: the lights won’t turn on. Of course, you would next ask the question: “Why won’t the light turn on?”

A hypothesis is a suggested explanation that can be tested. A hypothesis is NOT the question you are trying to answer – it is what you think the answer to the question will be and why . Several hypotheses may be proposed as answers to one question. For example, one hypothesis about the question “Why won’t the light turn on?” is “The light won’t turn on because the bulb is burned out.” There are also other possible answers to the question, and therefore other hypotheses may be proposed. A second hypothesis is “The light won’t turn on because the lamp is unplugged” or “The light won’t turn on because the power is out.” A hypothesis should be based on credible background information. A hypothesis is NOT just a guess (not even an educated one), although it can be based on your prior experience (such as in the example where the light won’t turn on). In general, hypotheses in biology should be based on a credible, referenced source of information.

A hypothesis must be testable to ensure that it is valid. For example, a hypothesis that depends on what a dog thinks is not testable, because we can’t tell what a dog thinks. It should also be falsifiable, meaning that it can be disproven by experimental results. An example of an unfalsifiable hypothesis is “Red is a better color than blue.” There is no experiment that might show this statement to be false. To test a hypothesis, a researcher will conduct one or more experiments designed to eliminate one or more of the hypotheses. This is important: a hypothesis can be disproven, or eliminated, but it can never be proven. If an experiment fails to disprove a hypothesis, then that explanation (the hypothesis) is supported as the answer to the question. However, that doesn’t mean that later on, we won’t find a better explanation or design a better experiment that will disprove the first hypothesis and lead to a better one.

A variable is any part of the experiment that can vary or change during the experiment. Typically, an experiment only tests one variable and all the other conditions in the experiment are held constant.

The variable that is being changed or tested is known as the independent variable .
The dependent variable is the thing (or things) that you are measuring as the outcome of your experiment.
A constant is a condition that is the same between all of the tested groups.
A confounding variable is a condition that is not held constant that could affect the experimental results.

Let’s start with the first hypothesis given above for the light bulb experiment: the bulb is burned out. When testing this hypothesis, the independent variable (the thing that you are testing) would be changing the light bulb and the dependent variable is whether or not the light turns on.

HINT: You should be able to put your identified independent and dependent variables into the phrase “dependent depends on independent”. If you say “whether or not the light turns on depends on changing the light bulb” this makes sense and describes this experiment. In contrast, if you say “changing the light bulb depends on whether or not the light turns on” it doesn’t make sense.

It would be important to hold all the other aspects of the environment constant, for example not messing with the lamp cord or trying to turn the lamp on using a different light switch. If the entire house had lost power during the experiment because a car hit the power pole, that would be a confounding variable.

You may have learned that a hypothesis can be phrased as an “If..then…” statement. Simple hypotheses can be phrased that way (but they must always also include a “because”), but more complicated hypotheses may require several sentences. It is also very easy to get confused by trying to put your hypothesis into this format. Don’t worry about phrasing hypotheses as “if…then” statements – that is almost never done in experiments outside a classroom.

The results of your experiment are the data that you collect as the outcome. In the light experiment, your results are either that the light turns on or the light doesn’t turn on. Based on your results, you can make a conclusion. Your conclusion uses the results to answer your original question.

flow chart illustrating a simplified version of the scientific process.

We can put the experiment with the light that won’t go in into the figure above:

Observation: the light won’t turn on.
Question: why won’t the light turn on?
Hypothesis: the lightbulb is burned out.
Prediction: if I change the lightbulb (independent variable), then the light will turn on (dependent variable).
Experiment: change the lightbulb while leaving all other variables the same.
Analyze the results: the light didn’t turn on.
Conclusion: The lightbulb isn’t burned out. The results do not support the hypothesis, time to develop a new one!
Hypothesis 2: the lamp is unplugged.
Prediction 2: if I plug in the lamp, then the light will turn on.
Experiment: plug in the lamp
Analyze the results: the light turned on!
Conclusion: The light wouldn’t turn on because the lamp was unplugged. The results support the hypothesis, it’s time to move on to the next experiment!

A more complex flow chart illustrating how the scientific method usually happens.

Control Groups

Another important aspect of designing an experiment is the presence of one or more control groups. A control group allows you to make a comparison that is important for interpreting your results. Control groups are samples that help you to determine that differences between your experimental groups are due to your treatment rather than a different variable – they eliminate alternate explanations for your results (including experimental error and experimenter bias). They increase reliability, often through the comparison of control measurements and measurements of the experimental groups. Often, the control group is a sample that is not treated with the independent variable, but is otherwise treated the same way as your experimental sample. This type of control group is treated the same way as the experimental group except it does not get treated with the independent variable. Therefore, if the results of the experimental group differ from the control group, the difference must be due to the change of the independent, rather than some outside factor. It is common in complex experiments (such as those published in scientific journals) to have more control groups than experimental groups.

Question: Which fertilizer will produce the greatest number of tomatoes when applied to the plants?

Hypothesis : If I apply different brands of fertilizer to tomato plants, the most tomatoes will be produced from plants watered with Brand A because Brand A advertises that it produces twice as many tomatoes as other leading brands.

Experiment: Purchase 10 tomato plants of the same type from the same nursery. Pick plants that are similar in size and age. Divide the plants into two groups of 5. Apply Brand A to the first group and Brand B to the second group according to the instructions on the packages. After 10 weeks, count the number of tomatoes on each plant.

Independent Variable: Brand of fertilizer.

Dependent Variable : Number of tomatoes.

The number of tomatoes produced depends on the brand of fertilizer applied to the plants.

Constants: amount of water, type of soil, size of pot, amount of light, type of tomato plant, length of time plants were grown.

Confounding variables : any of the above that are not held constant, plant health, diseases present in the soil or plant before it was purchased.

Results: Tomatoes fertilized with Brand A produced an average of 20 tomatoes per plant, while tomatoes fertilized with Brand B produced an average of 10 tomatoes per plant.

You’d want to use Brand A next time you grow tomatoes, right? But what if I told you that plants grown without fertilizer produced an average of 30 tomatoes per plant! Now what will you use on your tomatoes?

Results including control group : Tomatoes which received no fertilizer produced more tomatoes than either brand of fertilizer.

Conclusion: Although Brand A fertilizer produced more tomatoes than Brand B, neither fertilizer should be used because plants grown without fertilizer produced the most tomatoes!

More examples of control groups:

You observe growth . Does this mean that your spinach is really contaminated? Consider an alternate explanation for growth: the swab, the water, or the plate is contaminated with bacteria. You could use a control group to determine which explanation is true. If you wet one of the swabs and wiped on a nutrient plate, do bacteria grow?
You don’t observe growth. Does this mean that your spinach is really safe? Consider an alternate explanation for no growth: Salmonella isn’t able to grow on the type of nutrient you used in your plates. You could use a control group to determine which explanation is true. If you wipe a known sample of Salmonella bacteria on the plate, do bacteria grow?
You see a reduction in disease symptoms: you might expect a reduction in disease symptoms purely because the person knows they are taking a drug so they believe should be getting better. If the group treated with the real drug does not show more a reduction in disease symptoms than the placebo group, the drug doesn’t really work. The placebo group sets a baseline against which the experimental group (treated with the drug) can be compared.
You don’t see a reduction in disease symptoms: your drug doesn’t work. You don’t need an additional control group for comparison.
You would want a “placebo feeder”. This would be the same type of feeder, but with no food in it. Birds might visit a feeder just because they are interested in it; an empty feeder would give a baseline level for bird visits.
You would want a control group where you knew the enzyme would function. This would be a tube where you did not change the pH. You need this control group so you know your enzyme is working: if you didn’t see a reaction in any of the tubes with the pH adjusted, you wouldn’t know if it was because the enzyme wasn’t working at all or because the enzyme just didn’t work at any of your tested pH values.
You would also want a control group where you knew the enzyme would not function (no enzyme added). You need the negative control group so you can ensure that there is no reaction taking place in the absence of enzyme: if the reaction proceeds without the enzyme, your results are meaningless.

Text adapted from: OpenStax , Biology. OpenStax CNX. May 27, 2016 http://cnx.org/contents/[email protected]:RD6ERYiU@5/The-Process-of-Science .

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Microb Biotechnol
v.15(11); 2022 Nov

On the role of hypotheses in science

Harald brüssow.

1 Laboratory of Gene Technology, Department of Biosystems, KU Leuven, Leuven Belgium

Associated Data

Scientific research progresses by the dialectic dialogue between hypothesis building and the experimental testing of these hypotheses. Microbiologists as biologists in general can rely on an increasing set of sophisticated experimental methods for hypothesis testing such that many scientists maintain that progress in biology essentially comes with new experimental tools. While this is certainly true, the importance of hypothesis building in science should not be neglected. Some scientists rely on intuition for hypothesis building. However, there is also a large body of philosophical thinking on hypothesis building whose knowledge may be of use to young scientists. The present essay presents a primer into philosophical thoughts on hypothesis building and illustrates it with two hypotheses that played a major role in the history of science (the parallel axiom and the fifth element hypothesis). It continues with philosophical concepts on hypotheses as a calculus that fits observations (Copernicus), the need for plausibility (Descartes and Gilbert) and for explicatory power imposing a strong selection on theories (Darwin, James and Dewey). Galilei introduced and James and Poincaré later justified the reductionist principle in hypothesis building. Waddington stressed the feed‐forward aspect of fruitful hypothesis building, while Poincaré called for a dialogue between experiment and hypothesis and distinguished false, true, fruitful and dangerous hypotheses. Theoretical biology plays a much lesser role than theoretical physics because physical thinking strives for unification principle across the universe while biology is confronted with a breathtaking diversity of life forms and its historical development on a single planet. Knowledge of the philosophical foundations on hypothesis building in science might stimulate more hypothesis‐driven experimentation that simple observation‐oriented “fishing expeditions” in biological research.

Short abstract

Scientific research progresses by the dialectic dialogue between hypothesis building and the experimental testing of these hypotheses. Microbiologists can rely on an increasing set of sophisticated experimental methods for hypothesis testing but the importance of hypothesis building in science should not be neglected. This Lilliput offers a primer on philosophical concepts on hypotheses in science.

INTRODUCTION

Philosophy of science and the theory of knowledge (epistemology) are important branches of philosophy. However, philosophy has over the centuries lost its dominant role it enjoyed in antiquity and became in Medieval Ages the maid of theology (ancilla theologiae) and after the rise of natural sciences and its technological applications many practising scientists and the general public doubt whether they need philosophical concepts in their professional and private life. This is in the opinion of the writer of this article, an applied microbiologist, shortsighted for several reasons. Philosophers of the 20th century have made important contributions to the theory of knowledge, and many eminent scientists grew interested in philosophical problems. Mathematics which plays such a prominent role in physics and increasingly also in other branches of science is a hybrid: to some extent, it is the paradigm of an exact science while its abstract aspects are deeply rooted in philosophical thinking. In the present essay, the focus is on hypothesis and hypothesis building in science, essentially it is a compilation what philosophers and scientists thought about this subject in past and present. The controversy between the mathematical mind and that of the practical mind is an old one. The philosopher, physicist and mathematician Pascal ( 1623 –1662a) wrote in his Pensées : “Mathematicians who are only mathematicians have exact minds, provided all things are explained to them by means of definitions and axioms; otherwise they are inaccurate. They are only right when the principles are quite clear. And men of intuition cannot have the patience to reach to first principles of things speculative and conceptional, which they have never seen in the world and which are altogether out of the common. The intellect can be strong and narrow, and can be comprehensive and weak.” Hypothesis building is an act both of intuition and exact thinking and I hope that theoretical knowledge about hypothesis building will also profit young microbiologists.

HYPOTHESES AND AXIOMS IN MATHEMATICS

In the following, I will illustrate the importance of hypothesis building for the history of science and the development of knowledge and illustrate it with two famous concepts, the parallel axiom in mathematics and the five elements hypothesis in physics.

Euclidean geometry

The prominent role of hypotheses in the development of science becomes already clear in the first science book of the Western civilization: Euclid's The Elements written about 300 BC starts with a set of statements called Definitions, Postulates and Common Notions that lay out the foundation of geometry (Euclid, c.323‐c.283 ). This axiomatic approach is very modern as exemplified by the fact that Euclid's book remained for long time after the Bible the most read book in the Western hemisphere and a backbone of school teaching in mathematics. Euclid's twenty‐three definitions start with sentences such as “1. A point is that which has no part; 2. A line is breadthless length; 3. The extremities of a line are points”; and continues with the definition of angles (“8. A plane angle is the inclination to one another of two lines in a plane which meet one another and do not lie in a straight line”) and that of circles, triangles and quadrilateral figures. For the history of science, the 23rd definition of parallels is particularly interesting: “Parallel straight lines are straight lines which, being in the same plane and being produced indefinitely in both directions, do not meet one another in either direction”. This is the famous parallel axiom. It is clear that the parallel axiom cannot be the result of experimental observations, but must be a concept created in the mind. Euclid ends with five Common Notions (“1. Things which are equal to the same thing are also equal to one another, to 5. The whole is greater than the part”). The establishment of a contradiction‐free system for a branch of mathematics based on a set of axioms from which theorems were deduced was revolutionary modern. Hilbert ( 1899 ) formulated a sound modern formulation for Euclidian geometry. Hilbert's axiom system contains the notions “point, line and plane” and the concepts of “betweenness, containment and congruence” leading to five axioms, namely the axioms of Incidence (“Verknüpfung”), of Order (“Anordnung”), of Congruence, of Continuity (“Stetigkeit”) and of Parallels.

Origin of axioms

Philosophers gave various explanations for the origin of the Euclidean hypotheses or axioms. Plato considered geometrical figures as related to ideas (the true things behind the world of appearances). Aristoteles considered geometric figures as abstractions of physical bodies. Descartes perceived geometric figures as inborn ideas from extended bodies ( res extensa ), while Pascal thought that the axioms of Euclidian geometry were derived from intuition. Kant reasoned that Euclidian geometry represented a priori perceptions of space. Newton considered geometry as part of general mechanics linked to theories of measurement. Hilbert argued that the axioms of mathematical geometry are neither the result of contemplation (“Anschauung”) nor of psychological source. For him, axioms were formal propositions (“formale Aussageformen”) characterized by consistency (“Widerspruchsfreiheit”, i.e. absence of contradiction) (Mittelstrass, 1980a ).

Definitions

Axioms were also differently defined by philosophers. In Topics , Aristoteles calls axioms the assumptions taken up by one partner of a dialogue to initiate a dialectic discussion. Plato states that an axiom needs to be an acceptable or credible proposition, which cannot be justified by reference to other statements. Yet, a justification is not necessary because an axiom is an evident statement. In modern definition, axioms are methodical first sentences in the foundation of a deductive science (Mittelstrass, 1980a ). In Posterior Analytics , Aristotle defines postulates as positions which are at least initially not accepted by the dialogue partners while hypotheses are accepted for the sake of reasoning. In Euclid's book, postulates are construction methods that assure the existence of the geometric objects. Today postulates and axioms are used as synonyms while the 18th‐century philosophy made differences: Lambert defined axioms as descriptive sentences and postulates as prescriptive sentences. According to Kant, mathematical postulates create (synthesize) concepts (Mittelstrass, 1980b ). Definitions then fix the use of signs; they can be semantic definitions that explain the proper meaning of a sign in common language use (in a dictionary style) or they can be syntactic definitions that regulate the use of these signs in formal operations. Nominal definitions explain the words, while real definitions explain the meaning or the nature of the defined object. Definitions are thus essential for the development of a language of science, assuring communication and mutual understanding (Mittelstrass, 1980c ). Finally, hypotheses are also frequently defined as consistent conjectures that are compatible with the available knowledge. The truth of the hypothesis is only supposed in order to explain true observations and facts. Consequences of this hypothetical assumptions should explain the observed facts. Normally, descriptive hypotheses precede explanatory hypotheses in the development of scientific thought. Sometimes only tentative concepts are introduced as working hypotheses to test whether they have an explanatory capacity for the observations (Mittelstrass, 1980d ).

The Euclidian geometry is constructed along a logical “if→then” concept. The “if‐clause” formulates at the beginning the supposition, the “then clause” formulates the consequences from these axioms which provides a system of geometric theorems or insights. The conclusions do not follow directly from the hypothesis; this would otherwise represent self‐evident immediate conclusions. The “if‐then” concept in geometry is not used as in other branches of science where the consequences deduced from the axioms are checked against reality whether they are true, in order to confirm the validity of the hypothesis. The task in mathematics is: what can be logically deduced from a given set of axioms to build a contradiction‐free system of geometry. Whether this applies to the real world is in contrast to the situation in natural sciences another question and absolutely secondary to mathematics (Syntopicon, 1992 ).

Pascal's rules for hypotheses

In his Scientific Treatises on Geometric Demonstrations , Pascal ( 1623‐1662b ) formulates “Five rules are absolutely necessary and we cannot dispense with them without an essential defect and frequently even error. Do not leave undefined any terms at all obscure or ambiguous. Use in definitions of terms only words perfectly well known or already explained. Do not fail to ask that each of the necessary principles be granted, however clear and evident it may be. Ask only that perfectly self‐evident things be granted as axioms. Prove all propositions, using for their proof only axioms that are perfectly self‐evident or propositions already demonstrated or granted. Never get caught in the ambiguity of terms by failing to substitute in thought the definitions which restrict or define them. One should accept as true only those things whose contradiction appears to be false. We may then boldly affirm the original statement, however incomprehensible it is.”

Kant's rules on hypotheses

Kant ( 1724–1804 ) wrote that the analysis described in his book The Critique of Pure Reason “has now taught us that all its efforts to extend the bounds of knowledge by means of pure speculation, are utterly fruitless. So much the wider field lies open to hypothesis; as where we cannot know with certainty, we are at liberty to make guesses and to form suppositions. Imagination may be allowed, under the strict surveillance of reason, to invent suppositions; but these must be based on something that is perfectly certain‐ and that is the possibility of the object. Such a supposition is termed a hypothesis. We cannot imagine or invent any object or any property of an object not given in experience and employ it in a hypothesis; otherwise we should be basing our chain of reasoning upon mere chimerical fancies and not upon conception of things. Thus, we have no right to assume of new powers, not existing in nature and consequently we cannot assume that there is any other kind of community among substances than that observable in experience, any kind of presence than that in space and any kind of duration than that in time. The conditions of possible experience are for reason the only conditions of the possibility of things. Otherwise, such conceptions, although not self‐contradictory, are without object and without application. Transcendental hypotheses are therefore inadmissible, and we cannot use the liberty of employing in the absence of physical, hyperphysical grounds of explanation because such hypotheses do not advance reason, but rather stop it in its progress. When the explanation of natural phenomena happens to be difficult, we have constantly at hand a transcendental ground of explanation, which lifts us above the necessity of investigating nature. The next requisite for the admissibility of a hypothesis is its sufficiency. That is it must determine a priori the consequences which are given in experience and which are supposed to follow from the hypothesis itself.” Kant stresses another aspect when dealing with hypotheses: “It is our duty to try to discover new objections, to put weapons in the hands of our opponent, and to grant him the most favorable position. We have nothing to fear from these concessions; on the contrary, we may rather hope that we shall thus make ourselves master of a possession which no one will ever venture to dispute.”

For Kant's analytical and synthetical judgements and Difference between philosophy and mathematics (Kant, Whitehead) , see Appendices S1 and S2 , respectively.

Poincaré on hypotheses

The mathematician‐philosopher Poincaré ( 1854 –1912a) explored the foundation of mathematics and physics in his book Science and Hypothesis . In the preface to the book, he summarizes common thinking of scientists at the end of the 19th century. “To the superficial observer scientific truth is unassailable, the logic of science is infallible, and if scientific men sometimes make mistakes, it is because they have not understood the rules of the game. Mathematical truths are derived from a few self‐evident propositions, by a chain of flawless reasoning, they are imposed not only by us, but on Nature itself. This is for the minds of most people the origin of certainty in science.” Poincaré then continues “but upon more mature reflection the position held by hypothesis was seen; it was recognized that it is as necessary to the experimenter as it is to the mathematician. And then the doubt arose if all these constructions are built on solid foundations.” However, “to doubt everything or to believe everything are two equally convenient solutions: both dispense with the necessity of reflection. Instead, we should examine with the utmost care the role of hypothesis; we shall then recognize not only that it is necessary, but that in most cases it is legitimate. We shall also see that there are several kinds of hypotheses; that some are verifiable and when once confirmed by experiment become truths of great fertility; that others may be useful to us in fixing our ideas; and finally that others are hypotheses only in appearance, and reduce to definitions or to conventions in disguise.” Poincaré argues that “we must seek mathematical thought where it has remained pure‐i.e. in arithmetic, in the proofs of the most elementary theorems. The process is proof by recurrence. We first show that a theorem is true for n = 1; we then show that if it is true for n –1 it is true for n; and we conclude that it is true for all integers. The essential characteristic of reasoning by recurrence is that it contains, condensed in a single formula, an infinite number of syllogisms.” Syllogism is logical argument that applies deductive reasoning to arrive at a conclusion. Poincaré notes “that here is a striking analogy with the usual process of induction. But an essential difference exists. Induction applied to the physical sciences is always uncertain because it is based on the belief in a general order of the universe, an order which is external to us. Mathematical induction‐ i.e. proof by recurrence – is on the contrary, necessarily imposed on us, because it is only the affirmation of a property of the mind itself. No doubt mathematical recurrent reasoning and physical inductive reasoning are based on different foundations, but they move in parallel lines and in the same direction‐namely, from the particular to the general.”

Non‐Euclidian geometry: from Gauss to Lobatschewsky

Mathematics is an abstract science that intrinsically does not request that the structures described reflect a physical reality. Paradoxically, mathematics is the language of physics since the founder of experimental physics Galilei used Euclidian geometry when exploring the laws of the free fall. In his 1623 treatise The Assayer , Galilei ( 1564 –1642a) famously formulated that the book of Nature is written in the language of mathematics, thus establishing a link between formal concepts in mathematics and the structure of the physical world. Euclid's parallel axiom played historically a prominent role for the connection between mathematical concepts and physical realities. Mathematicians had doubted that the parallel axiom was needed and tried to prove it. In Euclidian geometry, there is a connection between the parallel axiom and the sum of the angles in a triangle being two right angles. It is therefore revealing that the famous mathematician C.F. Gauss investigated in the early 19th century experimentally whether this Euclidian theorem applies in nature. He approached this problem by measuring the sum of angles in a real triangle by using geodetic angle measurements of three geographical elevations in the vicinity of Göttingen where he was teaching mathematics. He reportedly measured a sum of the angles in this triangle that differed from 180°. Gauss had at the same time also developed statistical methods to evaluate the accuracy of measurements. Apparently, the difference of his measured angles was still within the interval of Gaussian error propagation. He did not publish the reasoning and the results for this experiment because he feared the outcry of colleagues about this unorthodox, even heretical approach to mathematical reasoning (Carnap, 1891 ‐1970a). However, soon afterwards non‐Euclidian geometries were developed. In the words of Poincaré, “Lobatschewsky assumes at the outset that several parallels may be drawn through a point to a given straight line, and he retains all the other axioms of Euclid. From these hypotheses he deduces a series of theorems between which it is impossible to find any contradiction, and he constructs a geometry as impeccable in its logic as Euclidian geometry. The theorems are very different, however, from those to which we are accustomed, and at first will be found a little disconcerting. For instance, the sum of the angles of a triangle is always less than two right angles, and the difference between that sum and two right angles is proportional to the area of the triangle. Lobatschewsky's propositions have no relation to those of Euclid, but are none the less logically interconnected.” Poincaré continues “most mathematicians regard Lobatschewsky's geometry as a mere logical curiosity. Some of them have, however, gone further. If several geometries are possible, they say, is it certain that our geometry is true? Experiments no doubt teaches us that the sum of the angles of a triangle is equal to two right angles, but this is because the triangles we deal with are too small” (Poincaré, 1854 ‐1912a)—hence the importance of Gauss' geodetic triangulation experiment. Gauss was aware that his three hills experiment was too small and thought on measurements on triangles formed with stars.

Poincaré vs. Einstein

Lobatschewsky's hyperbolic geometry did not remain the only non‐Euclidian geometry. Riemann developed a geometry without the parallel axiom, while the other Euclidian axioms were maintained with the exception of that of Order (Anordnung). Poincaré notes “so there is a kind of opposition between the geometries. For instance the sum of the angles in a triangle is equal to two right angles in Euclid's geometry, less than two right angles in that of Lobatschewsky, and greater than two right angles in that of Riemann. The number of parallel lines that can be drawn through a given point to a given line is one in Euclid's geometry, none in Riemann's, and an infinite number in the geometry of Lobatschewsky. Let us add that Riemann's space is finite, although unbounded.” As further distinction, the ratio of the circumference to the diameter of a circle is equal to π in Euclid's, greater than π in Lobatschewsky's and smaller than π in Riemann's geometry. A further difference between these geometries concerns the degree of curvature (Krümmungsmass k) which is 0 for a Euclidian surface, smaller than 0 for a Lobatschewsky and greater than 0 for a Riemann surface. The difference in curvature can be roughly compared with plane, concave and convex surfaces. The inner geometric structure of a Riemann plane resembles the surface structure of a Euclidean sphere and a Lobatschewsky plane resembles that of a Euclidean pseudosphere (a negatively curved geometry of a saddle). What geometry is true? Poincaré asked “Ought we then, to conclude that the axioms of geometry are experimental truths?” and continues “If geometry were an experimental science, it would not be an exact science. The geometric axioms are therefore neither synthetic a priori intuitions as affirmed by Kant nor experimental facts. They are conventions. Our choice among all possible conventions is guided by experimental facts; but it remains free and is only limited by the necessity of avoiding contradictions. In other words, the axioms of geometry are only definitions in disguise. What then are we to think of the question: Is Euclidean geometry true? It has no meaning. One geometry cannot be more true than another, it can only be more convenient. Now, Euclidean geometry is, and will remain, the most convenient, 1 st because it is the simplest and 2 nd because it sufficiently agrees with the properties of natural bodies” (Poincaré, 1854 ‐1912a).

Poincaré's book was published in 1903 and only a few years later Einstein published his general theory of relativity ( 1916 ) where he used a non‐Euclidean, Riemann geometry and where he demonstrated a structure of space that deviated from Euclidean geometry in the vicinity of strong gravitational fields. And in 1919, astronomical observations during a solar eclipse showed that light rays from a distant star were indeed “bent” when passing next to the sun. These physical observations challenged the view of Poincaré, and we should now address some aspects of hypotheses in physics (Carnap, 1891 ‐1970b).

HYPOTHESES IN PHYSICS

The long life of the five elements hypothesis.

Physical sciences—not to speak of biological sciences — were less developed in antiquity than mathematics which is already demonstrated by the primitive ideas on the elements constituting physical bodies. Plato and Aristotle spoke of the four elements which they took over from Thales (water), Anaximenes (air) and Parmenides (fire and earth) and add a fifth element (quinta essentia, our quintessence), namely ether. Ether is imagined a heavenly element belonging to the supralunar world. In Plato's dialogue Timaios (Plato, c.424‐c.348 BC a ), the five elements were associated with regular polyhedra in geometry and became known as Platonic bodies: tetrahedron (fire), octahedron (air), cube (earth), icosahedron (water) and dodecahedron (ether). In regular polyhedra, faces are congruent (identical in shape and size), all angles and all edges are congruent, and the same number of faces meet at each vertex. The number of elements is limited to five because in Euclidian space there are exactly five regular polyhedral. There is in Plato's writing even a kind of geometrical chemistry. Since two octahedra (air) plus one tetrahedron (fire) can be combined into one icosahedron (water), these “liquid” elements can combine while this is not the case for combinations with the cube (earth). The 12 faces of the dodecahedron were compared with the 12 zodiac signs (Mittelstrass, 1980e ). This geometry‐based hypothesis of physics had a long life. As late as 1612, Kepler in his Mysterium cosmographicum tried to fit the Platonic bodies into the planetary shells of his solar system model. The ether theory even survived into the scientific discussion of the 19th‐century physics and the idea of a mathematical structure of the universe dominated by symmetry operations even fertilized 20th‐century ideas about symmetry concepts in the physics of elementary particles.

Huygens on sound waves in air

The ether hypothesis figures prominently in the 1690 Treatise on Light from Huygens ( 1617‐1670 ). He first reports on the transmission of sound by air when writing “this may be proved by shutting up a sounding body in a glass vessel from which the air is withdrawn and care was taken to place the sounding body on cotton that it cannot communicate its tremor to the glass vessel which encloses it. After having exhausted all the air, one hears no sound from the metal though it is struck.” Huygens comes up with some foresight when suspecting “the air is of such a nature that it can be compressed and reduced to a much smaller space than that it normally occupies. Air is made up of small bodies which float about and which are agitated very rapidly. So that the spreading of sound is the effort which these little bodies make in collisions with one another, to regain freedom when they are a little more squeezed together in the circuit of these waves than elsewhere.”

Huygens on light waves in ether

“That is not the same air but another kind of matter in which light spreads; since if the air is removed from the vessel the light does not cease to traverse it as before. The extreme velocity of light cannot admit such a propagation of motion” as sound waves. To achieve the propagation of light, Huygens invokes ether “as a substance approaching to perfect hardness and possessing springiness as prompt as we choose. One may conceive light to spread successively by spherical waves. The propagation consists nowise in the transport of those particles but merely in a small agitation which they cannot help communicate to those surrounding.” The hypothesis of an ether in outer space fills libraries of physical discussions, but all experimental approaches led to contradictions with respect to postulated properties of this hypothetical material for example when optical experiments showed that light waves display transversal and not longitudinal oscillations.

The demise of ether

Mechanical models for the transmission of light or gravitation waves requiring ether were finally put to rest by the theory of relativity from Einstein (Mittelstrass, 1980f ). This theory posits that the speed of light in an empty space is constant and does not depend on movements of the source of light or that of an observer as requested by the ether hypothesis. The theory of relativity also provides an answer how the force of gravitation is transmitted from one mass to another across an essentially empty space. In the non‐Euclidian formulation of the theory of relativity (Einstein used the Riemann geometry), there is no gravitation force in the sense of mechanical or electromagnetic forces. The gravitation force is in this formulation simply replaced by a geometric structure (space curvature near high and dense masses) of a four‐dimensional space–time system (Carnap, 1891 ‐1970c; Einstein & Imfeld, 1956 ) Gravitation waves and gravitation lens effects have indeed been experimental demonstrated by astrophysicists (Dorfmüller et al., 1998 ).

For Aristotle's on physical hypotheses , see Appendix S3 .

PHILOSOPHICAL THOUGHTS ON HYPOTHESES

In the following, the opinions of a number of famous scientists and philosophers on hypotheses are quoted to provide a historical overview on the subject.

Copernicus' hypothesis: a calculus which fits observations

In his book Revolutions of Heavenly Spheres Copernicus ( 1473–1543 ) reasoned in the preface about hypotheses in physics. “Since the newness of the hypotheses of this work ‐which sets the earth in motion and puts an immovable sun at the center of the universe‐ has already received a great deal of publicity, I have no doubt that certain of the savants have taken great offense.” He defended his heliocentric thesis by stating “For it is the job of the astronomer to use painstaking and skilled observations in gathering together the history of the celestial movements‐ and then – since he cannot by any line of reasoning reach the true causes of these movements‐ to think up or construct whatever causes or hypotheses he pleases such that, by the assumption of these causes, those same movements can be calculated from the principles of geometry for the past and the future too. This artist is markedly outstanding in both of these respects: for it is not necessary that these hypotheses should be true, or even probable; but it is enough if they provide a calculus which fits the observations.” This preface written in 1543 sounds in its arguments very modern physics. However, historians of science have discovered that it was probably written by a theologian friend of Copernicus to defend the book against the criticism by the church.

Bacon's intermediate hypotheses

In his book Novum Organum , Francis Bacon ( 1561–1626 ) claims for hypotheses and scientific reasoning “that they augur well for the sciences, when the ascent shall proceed by a true scale and successive steps, without interruption or breach, from particulars to the lesser axioms, thence to the intermediates and lastly to the most general.” He then notes “that the lowest axioms differ but little from bare experiments, the highest and most general are notional, abstract, and of no real weight. The intermediate are true, solid, full of life, and up to them depend the business and fortune of mankind.” He warns that “we must not then add wings, but rather lead and ballast to the understanding, to prevent its jumping and flying, which has not yet been done; but whenever this takes place we may entertain greater hopes of the sciences.” With respect to methodology, Bacon claims that “we must invent a different form of induction. The induction which proceeds by simple enumeration is puerile, leads to uncertain conclusions, …deciding generally from too small a number of facts. Sciences should separate nature by proper rejections and exclusions and then conclude for the affirmative, after collecting a sufficient number of negatives.”

Gilbert and Descartes for plausible hypotheses

William Gilbert introduced in his book On the Loadstone (Gilbert, 1544‐1603 ) the argument of plausibility into physical hypothesis building. “From these arguments, therefore, we infer not with mere probability, but with certainty, the diurnal rotation of the earth; for nature ever acts with fewer than with many means; and because it is more accordant to reason that the one small body, the earth, should make a daily revolution than the whole universe should be whirled around it.”

Descartes ( 1596‐1650 ) reflected on the sources of understanding in his book Rules for Direction and distinguished what “comes about by impulse, by conjecture, or by deduction. Impulse can assign no reason for their belief and when determined by fanciful disposition, it is almost always a source of error.” When speaking about the working of conjectures he quotes thoughts of Aristotle: “water which is at a greater distance from the center of the globe than earth is likewise less dense substance, and likewise the air which is above the water, is still rarer. Hence, we hazard the guess that above the air nothing exists but a very pure ether which is much rarer than air itself. Moreover nothing that we construct in this way really deceives, if we merely judge it to be probable and never affirm it to be true; in fact it makes us better instructed. Deduction is thus left to us as the only means of putting things together so as to be sure of their truth. Yet in it, too, there may be many defects.”

Care in formulating hypotheses

Locke ( 1632‐1704 ) in his treatise Concerning Human Understanding admits that “we may make use of any probable hypotheses whatsoever. Hypotheses if they are well made are at least great helps to the memory and often direct us to new discoveries. However, we should not take up any one too hastily.” Also, practising scientists argued against careless use of hypotheses and proposed remedies. Lavoisier ( 1743‐1794 ) in the preface to his Element of Chemistry warned about beaten‐track hypotheses. “Instead of applying observation to the things we wished to know, we have chosen rather to imagine them. Advancing from one ill‐founded supposition to another, we have at last bewildered ourselves amidst a multitude of errors. These errors becoming prejudices, are adopted as principles and we thus bewilder ourselves more and more. We abuse words which we do not understand. There is but one remedy: this is to forget all that we have learned, to trace back our ideas to their sources and as Bacon says to frame the human understanding anew.”

Faraday ( 1791–1867 ) in a Speculation Touching Electric Conduction and the Nature of Matter highlighted the fundamental difference between hypotheses and facts when noting “that he has most power of penetrating the secrets of nature, and guessing by hypothesis at her mode of working, will also be most careful for his own safe progress and that of others, to distinguish that knowledge which consists of assumption, by which I mean theory and hypothesis, from that which is the knowledge of facts and laws; never raising the former to the dignity or authority of the latter.”

Explicatory power justifies hypotheses

Darwin ( 1809 –1882a) defended the conclusions and hypothesis of his book The Origin of Species “that species have been modified in a long course of descent. This has been affected chiefly through the natural selection of numerous, slight, favorable variations.” He uses a post hoc argument for this hypothesis: “It can hardly be supposed that a false theory would explain, to so satisfactory a manner as does the theory of natural selection, the several large classes of facts” described in his book.

The natural selection of hypotheses

In the concluding chapter of The Descent of Man Darwin ( 1809 –1882b) admits “that many of the views which have been advanced in this book are highly speculative and some no doubt will prove erroneous.” However, he distinguished that “false facts are highly injurious to the progress of science for they often endure long; but false views do little harm for everyone takes a salutory pleasure in proving their falseness; and when this is done, one path to error is closed and the road to truth is often at the same time opened.”

The American philosopher William James ( 1842–1907 ) concurred with Darwin's view when he wrote in his Principles of Psychology “every scientific conception is in the first instance a spontaneous variation in someone'’s brain. For one that proves useful and applicable there are a thousand that perish through their worthlessness. The scientific conceptions must prove their worth by being verified. This test, however, is the cause of their preservation, not of their production.”

The American philosopher J. Dewey ( 1859‐1952 ) in his treatise Experience and Education notes that “the experimental method of science attaches more importance not less to ideas than do other methods. There is no such thing as experiment in the scientific sense unless action is directed by some leading idea. The fact that the ideas employed are hypotheses, not final truths, is the reason why ideas are more jealously guarded and tested in science than anywhere else. As fixed truths they must be accepted and that is the end of the matter. But as hypotheses, they must be continuously tested and revised, a requirement that demands they be accurately formulated. Ideas or hypotheses are tested by the consequences which they produce when they are acted upon. The method of intelligence manifested in the experimental method demands keeping track of ideas, activities, and observed consequences. Keeping track is a matter of reflective review.”

The reductionist principle

James ( 1842‐1907 ) pushed this idea further when saying “Scientific thought goes by selection. We break the solid plenitude of fact into separate essences, conceive generally what only exists particularly, and by our classifications leave nothing in its natural neighborhood. The reality exists as a plenum. All its part are contemporaneous, but we can neither experience nor think this plenum. What we experience is a chaos of fragmentary impressions, what we think is an abstract system of hypothetical data and laws. We must decompose each chaos into single facts. We must learn to see in the chaotic antecedent a multitude of distinct antecedents, in the chaotic consequent a multitude of distinct consequents.” From these considerations James concluded “even those experiences which are used to prove a scientific truth are for the most part artificial experiences of the laboratory gained after the truth itself has been conjectured. Instead of experiences engendering the inner relations, the inner relations are what engender the experience here.“

Following curiosity

Freud ( 1856–1939 ) considered curiosity and imagination as driving forces of hypothesis building which need to be confronted as quickly as possible with observations. In Beyond the Pleasure Principle , Freud wrote “One may surely give oneself up to a line of thought and follow it up as far as it leads, simply out of scientific curiosity. These innovations were direct translations of observation into theory, subject to no greater sources of error than is inevitable in anything of the kind. At all events there is no way of working out this idea except by combining facts with pure imagination and thereby departing far from observation.” This can quickly go astray when trusting intuition. Freud recommends “that one may inexorably reject theories that are contradicted by the very first steps in the analysis of observation and be aware that those one holds have only a tentative validity.”

Feed‐forward aspects of hypotheses

The geneticist Waddington ( 1905–1975 ) in his essay The Nature of Life states that “a scientific theory cannot remain a mere structure within the world of logic, but must have implications for action and that in two rather different ways. It must involve the consequence that if you do so and so, such and such result will follow. That is to say it must give, or at least offer, the possibility of controlling the process. Secondly, its value is quite largely dependent on its power of suggesting the next step in scientific advance. Any complete piece of scientific work starts with an activity essentially the same as that of an artist. It starts by asking a relevant question. The first step may be a new awareness of some facet of the world that no one else had previously thought worth attending to. Or some new imaginative idea which depends on a sensitive receptiveness to the oddity of nature essentially similar to that of the artist. In his logical analysis and manipulative experimentation, the scientist is behaving arrogantly towards nature, trying to force her into his categories of thought or to trick her into doing what he wants. But finally he has to be humble. He has to take his intuition, his logical theory and his manipulative skill to the bar of Nature and see whether she answers yes or no; and he has to abide by the result. Science is often quite ready to tolerate some logical inadequacy in a theory‐or even a flat logical contradiction like that between the particle and wave theories of matter‐so long as it finds itself in the possession of a hypothesis which offers both the possibility of control and a guide to worthwhile avenues of exploration.”

Poincaré: the dialogue between experiment and hypothesis

Poincaré ( 1854 –1912b) also dealt with physics in Science and Hypothesis . “Experiment is the sole source of truth. It alone can teach us certainty. Cannot we be content with experiment alone? What place is left for mathematical physics? The man of science must work with method. Science is built up of facts, as a house is built of stones, but an accumulation of facts is no more a science than a heap of stones is a house. It is often said that experiments should be made without preconceived concepts. That is impossible. Without the hypothesis, no conclusion could have been drawn; nothing extraordinary would have been seen; and only one fact the more would have been catalogued, without deducing from it the remotest consequence.” Poincaré compares science to a library. Experimental physics alone can enrich the library with new books, but mathematical theoretical physics draw up the catalogue to find the books and to reveal gaps which have to be closed by the purchase of new books.

Poincaré: false, true, fruitful and dangerous hypotheses

Poincaré continues “we all know that there are good and bad experiments. The latter accumulate in vain. Whether there are hundred or thousand, one single piece of work will be sufficient to sweep them into oblivion. Bacon invented the term of an experimentum crucis for such experiments. What then is a good experiment? It is that which teaches us something more than an isolated fact. It is that which enables us to predict and to generalize. Experiments only gives us a certain number of isolated points. They must be connected by a continuous line and that is true generalization. Every generalization is a hypothesis. It should be as soon as possible submitted to verification. If it cannot stand the test, it must be abandoned without any hesitation. The physicist who has just given up one of his hypotheses should rejoice, for he found an unexpected opportunity of discovery. The hypothesis took into account all the known factors which seem capable of intervention in the phenomenon. If it is not verified, it is because there is something unexpected. Has the hypothesis thus rejected been sterile? Far from it. It has rendered more service than a true hypothesis.” Poincaré notes that “with a true hypothesis only one fact the more would have been catalogued, without deducing from it the remotest consequence. It may be said that the wrong hypothesis has rendered more service than a true hypothesis.” However, Poincaré warns that “some hypotheses are dangerous – first and foremost those which are tacit and unconscious. And since we make them without knowing them, we cannot get rid of them.” Poincaré notes that here mathematical physics is of help because by its precision one is compelled to formulate all the hypotheses, revealing also the tacit ones.

Arguments for the reductionist principle

Poincaré also warned against multiplying hypotheses indefinitely: “If we construct a theory upon multiple hypotheses, and if experiment condemns it, which of the premisses must be changed?” Poincaré also recommended to “resolve the complex phenomenon given directly by experiment into a very large number of elementary phenomena. First, with respect to time. Instead of embracing in its entirety the progressive development of a phenomenon, we simply try to connect each moment with the one immediately preceding. Next, we try to decompose the phenomenon in space. We must try to deduce the elementary phenomenon localized in a very small region of space.” Poincaré suggested that the physicist should “be guided by the instinct of simplicity, and that is why in physical science generalization so readily takes the mathematical form to state the problem in the form of an equation.” This argument goes back to Galilei ( 1564 –1642b) who wrote in The Two Sciences “when I observe a stone initially at rest falling from an elevated position and continually acquiring new increments of speed, why should I not believe that such increases take place in a manner which is exceedingly simple and rather obvious to everybody? If now we examine the matter carefully we find no addition or increment more simple than that which repeats itself always in the same manner. It seems we shall not be far wrong if we put the increment of speed as proportional to the increment of time.” With a bit of geometrical reasoning, Galilei deduced that the distance travelled by a freely falling body varies as the square of the time. However, Galilei was not naïve and continued “I grant that these conclusions proved in the abstract will be different when applied in the concrete” and considers disturbances cause by friction and air resistance that complicate the initially conceived simplicity.

Four sequential steps of discovery…

Some philosophers of science attributed a fundamental importance to observations for the acquisition of experience in science. The process starts with accidental observations (Aristotle), going to systematic observations (Bacon), leading to quantitative rules obtained with exact measurements (Newton and Kant) and culminating in observations under artificially created conditions in experiments (Galilei) (Mittelstrass, 1980g ).

…rejected by Popper and Kant

In fact, Newton wrote that he had developed his theory of gravitation from experience followed by induction. K. Popper ( 1902‐1994 ) in his book Conjectures and Refutations did not agree with this logical flow “experience leading to theory” and that for several reasons. This scheme is according to Popper intuitively false because observations are always inexact, while theory makes absolute exact assertions. It is also historically false because Copernicus and Kepler were not led to their theories by experimental observations but by geometry and number theories of Plato and Pythagoras for which they searched verifications in observational data. Kepler, for example, tried to prove the concept of circular planetary movement influenced by Greek theory of the circle being a perfect geometric figure and only when he could not demonstrate this with observational data, he tried elliptical movements. Popper noted that it was Kant who realized that even physical experiments are not prior to theories when quoting Kant's preface to the Critique of Pure Reason : “When Galilei let his globes run down an inclined plane with a gravity which he has chosen himself, then a light dawned on all natural philosophers. They learnt that our reason can only understand what it creates according to its own design; that we must compel Nature to answer our questions, rather than cling to Nature's apron strings and allow her to guide us. For purely accidental observations, made without any plan having been thought out in advance, cannot be connected by a law‐ which is what reason is searching for.” From that reasoning Popper concluded that “we ourselves must confront nature with hypotheses and demand a reply to our questions; and that lacking such hypotheses, we can only make haphazard observations which follow no plan and which can therefore never lead to a natural law. Everyday experience, too, goes far beyond all observations. Everyday experience must interpret observations for without theoretical interpretation, observations remain blind and uninformative. Everyday experience constantly operates with abstract ideas, such as that of cause and effect, and so it cannot be derived from observation.” Popper agreed with Kant who said “Our intellect does not draw its laws from nature…but imposes them on nature”. Popper modifies this statement to “Our intellect does not draw its laws from nature, but tries‐ with varying degrees of success – to impose upon nature laws which it freely invents. Theories are seen to be free creations of our mind, the result of almost poetic intuition. While theories cannot be logically derived from observations, they can, however, clash with observations. This fact makes it possible to infer from observations that a theory is false. The possibility of refuting theories by observations is the basis of all empirical tests. All empirical tests are therefore attempted refutations.”

OUTLOOK: HYPOTHESES IN BIOLOGY

Is biology special.

Waddington notes that “living organisms are much more complicated than the non‐living things. Biology has therefore developed more slowly than sciences such as physics and chemistry and has tended to rely on them for many of its basic ideas. These older physical sciences have provided biology with many firm foundations which have been of the greatest value to it, but throughout most of its history biology has found itself faced with the dilemma as to how far its reliance on physics and chemistry should be pushed” both with respect to its experimental methods and its theoretical foundations. Vitalism is indeed such a theory maintaining that organisms cannot be explained solely by physicochemical laws claiming specific biological forces active in organisms. However, efforts to prove the existence of such vital forces have failed and today most biologists consider vitalism a superseded theory.

Biology as a branch of science is as old as physics. If one takes Aristotle as a reference, he has written more on biology than on physics. Sophisticated animal experiments were already conducted in the antiquity by Galen (Brüssow, 2022 ). Alertus Magnus displayed biological research interest during the medieval time. Knowledge on plants provided the basis of medical drugs in early modern times. What explains biology's decreasing influence compared with the rapid development of physics by Galilei and Newton? One reason is the possibility to use mathematical equations to describe physical phenomena which was not possible for biological phenomena. Physics has from the beginning displayed a trend to few fundamental underlying principles. This is not the case for biology. With the discovery of new continents, biologists were fascinated by the diversity of life. Diversity was the conducting line of biological thinking. This changed only when taxonomists and comparative anatomists revealed recurring pattern in this stunning biological variety and when Darwin provided a theoretical concept to understand variation as a driving force in biology. Even when genetics and molecular biology allowed to understand biology from a few universally shared properties, such as a universal genetic code, biology differed in fundamental aspects from physics and chemistry. First, biology is so far restricted to the planet earth while the laws of physic and chemistry apply in principle to the entire universe. Second, biology is to a great extent a historical discipline; many biological processes cannot be understood from present‐day observations because they are the result of historical developments in evolution. Hence, the importance of Dobzhansky's dictum that nothing makes sense in biology except in the light of evolution. The great diversity of life forms, the complexity of processes occurring in cells and their integration in higher organisms and the importance of a historical past for the understanding of extant organisms, all that has delayed the successful application of mathematical methods in biology or the construction of theoretical frameworks in biology. Theoretical biology by far did not achieve a comparable role as theoretical physics which is on equal foot with experimental physics. Many biologists are even rather sceptical towards a theoretical biology and see progress in the development of ever more sophisticated experimental methods instead in theoretical concepts expressed by new hypotheses.

Knowledge from data without hypothesis?

Philosophers distinguish rational knowledge ( cognitio ex principiis ) from knowledge from data ( cognitio ex data ). Kant associates these two branches with natural sciences and natural history, respectively. The latter with descriptions of natural objects as prominently done with systematic classification of animals and plants or, where it is really history, when describing events in the evolution of life forms on earth. Cognitio ex data thus played a much more prominent role in biology than in physics and explains why the compilation of data and in extremis the collection of museum specimen characterizes biological research. To account for this difference, philosophers of the logical empiricism developed a two‐level concept of science languages consisting of a language of observations (Beobachtungssprache) and a language of theories (Theoriesprache) which are linked by certain rules of correspondence (Korrespondenzregeln) (Carnap, 1891 –1970d). If one looks into leading biological research journals, it becomes clear that biology has a sophisticated language of observation and a much less developed language of theories.

Do we need more philosophical thinking in biology or at least a more vigorous theoretical biology? The breathtaking speed of progress in experimental biology seems to indicate that biology can well develop without much theoretical or philosophical thinking. At the same time, one could argue that some fields in biology might need more theoretical rigour. Microbiologists might think on microbiome research—one of the breakthrough developments of microbiology research in recent years. The field teems with fascinating, but ill‐defined terms (our second genome; holobionts; gut–brain axis; dysbiosis, symbionts; probiotics; health benefits) that call for stricter definitions. One might also argue that biologists should at least consider the criticism of Goethe ( 1749–1832 ), a poet who was also an active scientist. In Faust , the devil ironically teaches biology to a young student.

“Wer will was Lebendigs erkennen und beschreiben, Sucht erst den Geist herauszutreiben, Dann hat er die Teile in seiner Hand, Fehlt, leider! nur das geistige Band.” (To docket living things past any doubt. You cancel first the living spirit out: The parts lie in the hollow of your hand, You only lack the living thing you banned).

We probably need both in biology: more data and more theory and hypotheses.

CONFLICT OF INTEREST

The author reports no conflict of interest.

FUNDING INFORMATION

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Supporting information

Appendix S1

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Hypothesis Definition (Science)

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Ph.D., Biomedical Sciences, University of Tennessee at Knoxville
B.A., Physics and Mathematics, Hastings College

A hypothesis is an explanation that is proposed for a phenomenon. Formulating a hypothesis is a step of the scientific method .

Alternate Spellings: plural: hypotheses

Examples: Upon observing that a lake appears blue under a blue sky, you might propose the hypothesis that the lake is blue because it is reflecting the sky. One alternate hypothesis would be that the lake is blue because water is blue.

Hypothesis Versus Theory

Although in common usage the terms hypothesis and theory are used interchangeably, the two words mean something different from each other in science. Like a hypothesis, a theory is testable and may be used to make predictions. However, a theory has been tested using the scientific method many times. Testing a hypothesis may, over time, lead to the formulation of a theory.

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1.2: The Process of Science

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Photo A depicts round colonies of blue-green algae. Photo B depicts round fossil structures called stromatalites along a watery shoreline.

The methods of science include careful observation, record keeping, logical and mathematical reasoning, experimentation, and submitting conclusions to the scrutiny of others. Science also requires considerable imagination and creativity; a well-designed experiment is commonly described as elegant, or beautiful. Like politics, science has considerable practical implications and some science is dedicated to practical applications, such as the prevention of disease (Figure \(\PageIndex{2}\)). Other science proceeds largely motivated by curiosity. Whatever its goal, there is no doubt that science, including biology, has transformed human existence and will continue to do so.

Scanning electronic micrograph depicts E. coli bacteria aggregated together.

The Nature of Science

Science is a very specific way of learning, or knowing, about the world. The history of the past 500 years demonstrates that science is a very powerful way of knowing about the world; it is largely responsible for the technological revolutions that have taken place during this time. There are however, areas of knowledge and human experience that the methods of science cannot be applied to. These include such things as answering purely moral questions, aesthetic questions, or what can be generally categorized as spiritual questions. Science has cannot investigate these areas because they are outside the realm of material phenomena, the phenomena of matter and energy, and cannot be observed and measured.

The scientific method is a method of research with defined steps that include experiments and careful observation. The steps of the scientific method will be examined in detail later, but one of the most important aspects of this method is the testing of hypotheses. A hypothesis is a suggested explanation for an event, which can be tested. Hypotheses, or tentative explanations, are generally produced within the context of a scientific theory. A scientific theory is a generally accepted, thoroughly tested and confirmed explanation for a set of observations or phenomena. Scientific theory is the foundation of scientific knowledge. In addition, in many scientific disciplines (less so in biology) there are scientific laws, often expressed in mathematical formulas, which describe how elements of nature will behave under certain specific conditions. There is not an evolution of hypotheses through theories to laws as if they represented some increase in certainty about the world. Hypotheses are the day-to-day material that scientists work with and they are developed within the context of theories. Laws are concise descriptions of parts of the world that are amenable to formulaic or mathematical description.

Natural Sciences

What would you expect to see in a museum of natural sciences? Frogs? Plants? Dinosaur skeletons? Exhibits about how the brain functions? A planetarium? Gems and minerals? Or maybe all of the above? Science includes such diverse fields as astronomy, biology, computer sciences, geology, logic, physics, chemistry, and mathematics (Figure \(\PageIndex{3}\)). However, those fields of science related to the physical world and its phenomena and processes are considered natural sciences. Thus, a museum of natural sciences might contain any of the items listed above.

Some fields of science include astronomy, biology, computer science, geology, logic, physics, chemistry, and mathematics. (credit: "Image Editor/Flickr)"

There is no complete agreement when it comes to defining what the natural sciences include. For some experts, the natural sciences are astronomy, biology, chemistry, earth science, and physics. Other scholars choose to divide natural sciences into life sciences, which study living things and include biology, and physical sciences, which study nonliving matter and include astronomy, physics, and chemistry. Some disciplines such as biophysics and biochemistry build on two sciences and are interdisciplinary.

Scientific Inquiry

Deductive reasoning or deduction is the type of logic used in hypothesis-based science. In deductive reasoning, the pattern of thinking moves in the opposite direction as compared to inductive reasoning. Deductive reasoning is a form of logical thinking that uses a general principle or law to forecast specific results. From those general principles, a scientist can extrapolate and predict the specific results that would be valid as long as the general principles are valid. For example, a prediction would be that if the climate is becoming warmer in a region, the distribution of plants and animals should change. Comparisons have been made between distributions in the past and the present, and the many changes that have been found are consistent with a warming climate. Finding the change in distribution is evidence that the climate change conclusion is a valid one.

Hypothesis Testing

Biologists study the living world by posing questions about it and seeking science-based responses. This approach is common to other sciences as well and is often referred to as the scientific method. The scientific method was used even in ancient times, but it was first documented by England’s Sir Francis Bacon (1561–1626) (Figure \(\PageIndex{4}\)), who set up inductive methods for scientific inquiry. The scientific method is not exclusively used by biologists but can be applied to almost anything as a logical problem-solving method.

Painting depicts Sir Francis Bacon in a long cloak.

A hypothesis must be testable to ensure that it is valid. For example, a hypothesis that depends on what a bear thinks is not testable, because it can never be known what a bear thinks. It should also be falsifiable, meaning that it can be disproven by experimental results. An example of an unfalsifiable hypothesis is “Botticelli’s Birth of Venus is beautiful.” There is no experiment that might show this statement to be false. To test a hypothesis, a researcher will conduct one or more experiments designed to eliminate one or more of the hypotheses. This is important. A hypothesis can be disproven, or eliminated, but it can never be proven. Science does not deal in proofs like mathematics. If an experiment fails to disprove a hypothesis, then we find support for that explanation, but this is not to say that down the road a better explanation will not be found, or a more carefully designed experiment will be found to falsify the hypothesis.

Each experiment will have one or more variables and one or more controls. A variable is any part of the experiment that can vary or change during the experiment. A control is a part of the experiment that does not change. Look for the variables and controls in the example that follows. As a simple example, an experiment might be conducted to test the hypothesis that phosphate limits the growth of algae in freshwater ponds. A series of artificial ponds are filled with water and half of them are treated by adding phosphate each week, while the other half are treated by adding a salt that is known not to be used by algae. The variable here is the phosphate (or lack of phosphate), the experimental or treatment cases are the ponds with added phosphate and the control ponds are those with something inert added, such as the salt. Just adding something is also a control against the possibility that adding extra matter to the pond has an effect. If the treated ponds show lesser growth of algae, then we have found support for our hypothesis. If they do not, then we reject our hypothesis. Be aware that rejecting one hypothesis does not determine whether or not the other hypotheses can be accepted; it simply eliminates one hypothesis that is not valid (Figure \(\PageIndex{5}\)). Using the scientific method, the hypotheses that are inconsistent with experimental data are rejected.

A flow chart shows the steps in the scientific method. In step 1, an observation is made. In step 2, a question is asked about the observation. In step 3, an answer to the question, called a hypothesis, is proposed. In step 4, a prediction is made based on the hypothesis. In step 5, an experiment is done to test the prediction. In step 6, the results are analyzed to determine whether or not the hypothesis is supported. If the hypothesis is not supported, another hypothesis is made. In either case, the results are reported.

Example \(\PageIndex{1}\)

My toaster doesn’t toast my bread.
Why doesn’t my toaster work?
There is something wrong with the electrical outlet.
If something is wrong with the outlet, my coffeemaker also won’t work when plugged into it.
I plug my coffeemaker into the outlet.
My coffeemaker works.

The hypothesis is #3 (there is something wrong with the electrical outlet), and the prediction is #4 (if something is wrong with the outlet, then the coffeemaker also won’t work when plugged into the outlet). The original hypothesis is not supported, as the coffee maker works when plugged into the outlet. Alternative hypotheses may include (1) the toaster might be broken or (2) the toaster wasn’t turned on.

Basic and Applied Science

Another example of the link between basic and applied research is the Human Genome Project, a study in which each human chromosome was analyzed and mapped to determine the precise sequence of DNA subunits and the exact location of each gene. (The gene is the basic unit of heredity; an individual’s complete collection of genes is his or her genome.) Other organisms have also been studied as part of this project to gain a better understanding of human chromosomes. The Human Genome Project (Figure \(\PageIndex{6}\)) relied on basic research carried out with non-human organisms and, later, with the human genome. An important end goal eventually became using the data for applied research seeking cures for genetically related diseases.

Reporting Scientific Work

Biology is the science that studies living organisms and their interactions with one another and their environments. Science attempts to describe and understand the nature of the universe in whole or in part. Science has many fields; those fields related to the physical world and its phenomena are considered natural sciences.

A hypothesis is a tentative explanation for an observation. A scientific theory is a well-tested and consistently verified explanation for a set of observations or phenomena. A scientific law is a description, often in the form of a mathematical formula, of the behavior of an aspect of nature under certain circumstances. Two types of logical reasoning are used in science. Inductive reasoning uses results to produce general scientific principles. Deductive reasoning is a form of logical thinking that predicts results by applying general principles. The common thread throughout scientific research is the use of the scientific method. Scientists present their results in peer-reviewed scientific papers published in scientific journals.

Science can be basic or applied. The main goal of basic science is to expand knowledge without any expectation of short-term practical application of that knowledge. The primary goal of applied research, however, is to solve practical problems.

Contributors and Attributions

Samantha Fowler (Clayton State University), Rebecca Roush (Sandhills Community College), James Wise (Hampton University). Original content by OpenStax (CC BY 4.0; Access for free at https://cnx.org/contents/b3c1e1d2-83...4-e119a8aafbdd ).

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Dogs can understand the meaning of nouns, new research finds

Study confirms our canine companions can grasp more than simple commands – or at least for items they care about

Dogs understand what certain words stand for, according to researchers who monitored the brain activity of willing pooches while they were shown balls, slippers, leashes and other highlights of the domestic canine world.

The finding suggests that the dog brain can reach beyond commands such as “sit” and “fetch”, and the frenzy-inducing “walkies”, to grasp the essence of nouns, or at least those that refer to items the animals care about.

“I think the capacity is there in all dogs,” said Marianna Boros, who helped arrange the experiments at Eötvös Loránd University in Hungary. “This changes our understanding of language evolution and our sense of what is uniquely human.”

Scientists have long been fascinated by whether dogs can truly learn the meanings of words and have built up some evidence to back the suspicion. A survey in 2022 found that dog owners believed their furry companions responded to between 15 and 215 words.

More direct evidence for canine cognitive prowess came in 2011 when psychologists in South Carolina reported that after three years of intensive training, a border collie called Chaser had learned the names of more than 1,000 objects , including 800 cloth toys, 116 balls and 26 Frisbees.

However, studies have said little about what is happening in the canine brain when it processes words. To delve into the mystery, Boros and her colleagues invited 18 dog owners to bring their pets to the laboratory along with five objects the animals knew well. These included balls, slippers, Frisbees, rubber toys, leads and other items.

At the lab, the owners were instructed to say words for objects before showing their dog either the correct item or a different one. For example, an owner might say “Look, here’s the ball”, but hold up a Frisbee instead. The experiments were repeated multiple times with matching and non-matching objects.

During the tests, researchers monitored the dogs’ brain activity through non-invasive electroencephalography, or EEG. The traces revealed different patterns of activity when the objects matched or clashed with the words their owner said. The difference in the traces was more pronounced for words that owners believed their dogs knew best.

Similar blips in EEG recordings were seen when humans performed the tests and were interpreted as people understanding a word well enough to form a mental representation that was either confirmed or confounded by the object they were subsequently shown.

Writing in Current Biology , the scientists say the results “provide the first neural evidence for object word knowledge in a non-human animal”.

Boros emphasised she was not claiming dogs understood words as well as humans. It will take further work to understand, for example, whether dogs can generalise in the way humans learn to as infants, and grasp that the word “ball” need not refer to one specific, heavily chewed spongy sphere.

The study raises the question of why, if dogs understand certain nouns, more of them don’t show it. One possibility is that a dog knows what a word refers to but is not bothered about acting on it. “My dog only cares about his ball,” said Boros. “If I bring him another toy, he doesn’t care about it at all.”

Dr Holly Root-Gutteridge, a postdoctoral researcher at the University of Lincoln who was not involved in the study, called the work “fascinating”.

“It’s particularly interesting because I think it’s unlikely this started during domestication, so it may be widespread throughout mammals,” she said. “That’s highly exciting in itself as it shines new light on language evolution.

“It might be that the dogs don’t really care enough about the game of ‘fetch this particular thing’ to play along with the way we’ve been training and testing them so far. Your dog may understand what you’re saying but choose not to act.”

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Hypothesis
Biology definition: A hypothesis is a supposition or tentative explanation for (a group of) phenomena, (a set of) facts, or a scientific inquiry that may be tested, verified or answered by further investigation or methodological experiment.It is like a scientific guess.It's an idea or prediction that scientists make before they do experiments. They use it to guess what might happen and then ...
Scientific hypothesis
scientific hypothesis, an idea that proposes a tentative explanation about a phenomenon or a narrow set of phenomena observed in the natural world. The two primary features of a scientific hypothesis are falsifiability and testability, which are reflected in an "If…then" statement summarizing the idea and in the ability to be supported or ...
The scientific method (article)
The scientific method. At the core of biology and other sciences lies a problem-solving approach called the scientific method. The scientific method has five basic steps, plus one feedback step: Make an observation. Ask a question. Form a hypothesis, or testable explanation. Make a prediction based on the hypothesis.
Biology and the scientific method review
Meaning; Biology: The study of living things: Observation: Noticing and describing events in an orderly way: Hypothesis: A scientific explanation that can be tested through experimentation or observation: Controlled experiment: ... A hypothesis is not necessarily the right explanation. Instead, it is a possible explanation that can be tested to ...
What is a scientific hypothesis?
A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method. Many describe it as an "educated guess ...
What Is a Hypothesis? The Scientific Method
A hypothesis (plural hypotheses) is a proposed explanation for an observation. The definition depends on the subject. In science, a hypothesis is part of the scientific method. It is a prediction or explanation that is tested by an experiment. Observations and experiments may disprove a scientific hypothesis, but can never entirely prove one.
1.3: The Science of Biology
The scientific method can be applied to almost all fields of study as a logical, rational, problem-solving method. Figure 1.3.1 1.3. 1: Sir Francis Bacon: Sir Francis Bacon (1561-1626) is credited with being the first to define the scientific method. The scientific process typically starts with an observation (often a problem to be solved ...
4.14: Experiments and Hypotheses
Experiments and further observations are often used to test the hypotheses. A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always.
1.1 The Science of Biology
In simple terms, biology is the study of life. This is a very broad definition because the scope of biology is vast. Biologists may study anything from the microscopic or submicroscopic view of a cell to ecosystems and the whole living planet ( Figure 1.2 ). Listening to the daily news, you will quickly realize how many aspects of biology we ...
Hypothesis Definition & Meaning
hypothesis: [noun] an assumption or concession made for the sake of argument. an interpretation of a practical situation or condition taken as the ground for action.
Hypothesis
hypothesis, something supposed or taken for granted, with the object of following out its consequences (Greek hypothesis, "a putting under," the Latin equivalent being suppositio). Discussion with Kara Rogers of how the scientific model is used to test a hypothesis or represent a theory
Controlled experiments (article)
It looks like the "seeds need water" hypothesis is probably correct! Let's see how this simple example illustrates the parts of a controlled experiment. Panel 1: Two identical pots are prepared. 10 bean seeds are added to each pot. The pots are placed near the window. Panel 2: One pot (experimental group) is watered.
Hypothesis
The hypothesis of Andreas Cellarius, showing the planetary motions in eccentric and epicyclical orbits.. A hypothesis (pl.: hypotheses) is a proposed explanation for a phenomenon.For a hypothesis to be a scientific hypothesis, the scientific method requires that one can test it. Scientists generally base scientific hypotheses on previous observations that cannot satisfactorily be explained ...
How to Write a Strong Hypothesis
Developing a hypothesis (with example) Step 1. Ask a question. Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project. Example: Research question.
1.3: Scientific Theories
Scientific Theories. With repeated testing, some hypotheses may eventually become scientific theories. Keep in mind, a hypothesis is a possible answer to a scientific question. A scientific theory is a broad explanation for events that is widely accepted as true. To become a theory, a hypothesis must be tested over and over again, and it must be supported by a great deal of evidence.
1.2 The Process of Science
Like geology, physics, and chemistry, biology is a science that gathers knowledge about the natural world. Specifically, biology is the study of life. The discoveries of biology are made by a community of researchers who work individually and together using agreed-on methods. In this sense, biology, like all sciences is a social enterprise like ...
Hypothesis Testing
1 Hypothesis Testing . Biology is a science, but what exactly is science? What does the study of biology share with other scientific disciplines? Science (from the Latin scientia, meaning "knowledge") can be defined as knowledge about the natural world. Biologists study the living world by posing questions about it and seeking science-based responses.
On the role of hypotheses in science
Scientific research progresses by the dialectic dialogue between hypothesis building and the experimental testing of these hypotheses. Microbiologists as biologists in general can rely on an increasing set of sophisticated experimental methods for hypothesis testing such that many scientists maintain that progress in biology essentially comes with new experimental tools.
Theory vs. Hypothesis: Basics of the Scientific Method
Theory vs. Hypothesis: Basics of the Scientific Method. Written by MasterClass. Last updated: Jun 7, 2021 • 2 min read. Though you may hear the terms "theory" and "hypothesis" used interchangeably, these two scientific terms have drastically different meanings in the world of science.
Hypothesis Definition (Science)
A hypothesis is an explanation that is proposed for a phenomenon. Formulating a hypothesis is a step of the scientific method . Examples: Upon observing that a lake appears blue under a blue sky, you might propose the hypothesis that the lake is blue because it is reflecting the sky. One alternate hypothesis would be that the lake is blue ...
1.2: The Process of Science
A scientific theory is a well-tested and consistently verified explanation for a set of observations or phenomena. A scientific law is a description, often in the form of a mathematical formula, of the behavior of an aspect of nature under certain circumstances. Two types of logical reasoning are used in science.
Writing a hypothesis and prediction
A hypothesis is an idea about how something works that can be tested using experiments. A prediction says what will happen in an experiment if the hypothesis is correct. Presenter 1: We are going ...
Dogs can understand the meaning of nouns, new research finds
Scientists have long been fascinated by whether dogs can truly learn the meanings of words and have built up some evidence to back the suspicion. A survey in 2022 found that dog owners believed ...

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