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How to Write a Great Hypothesis

Hypothesis Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis, operational definitions, types of hypotheses, hypotheses examples.

• Collecting Data

Frequently Asked Questions

A hypothesis is a tentative statement about the relationship between two or more  variables. It is a specific, testable prediction about what you expect to happen in a study.

One hypothesis example would be a study designed to look at the relationship between sleep deprivation and test performance might have a hypothesis that states: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. It is only at this point that researchers begin to develop a testable hypothesis. Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore a number of factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk wisdom that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis.   In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in a number of different ways. One of the basic principles of any type of scientific research is that the results must be replicable.   By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. How would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

In order to measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming other people. In this situation, the researcher might utilize a simulated task to measure aggressiveness.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests that there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type of hypothesis suggests a relationship between three or more variables, such as two independent variables and a dependent variable.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative sample of the population and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• Complex hypothesis: "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "Children who receive a new reading intervention will have scores different than students who do not receive the intervention."
• "There will be no difference in scores on a memory recall task between children and adults."

Examples of an alternative hypothesis:

• "Children who receive a new reading intervention will perform better than students who did not receive the intervention."
• "Adults will perform better on a memory task than children." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when it would be impossible or difficult to  conduct an experiment . These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a correlational study can then be used to look at how the variables are related. This type of research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

A Word From Verywell

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Some examples of how to write a hypothesis include:

• "Staying up late will lead to worse test performance the next day."
• "People who consume one apple each day will visit the doctor fewer times each year."
• "Breaking study sessions up into three 20-minute sessions will lead to better test results than a single 60-minute study session."

The four parts of a hypothesis are:

• The research question
• The independent variable (IV)
• The dependent variable (DV)
• The proposed relationship between the IV and DV

Castillo M. The scientific method: a need for something better? . AJNR Am J Neuroradiol. 2013;34(9):1669-71. doi:10.3174/ajnr.A3401

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

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• Knowledge Base
• Methodology
• How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

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Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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Module 1: Introduction to Biology

Experiments and hypotheses, learning outcomes.

• Form a hypothesis and use it to design a scientific experiment

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis, and it must be possible to prove your hypothesis true or false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

Air pollution from automobile exhaust can trigger symptoms in people with asthma.

• No. This statement is not testable or falsifiable.
• No. This statement is not testable.
• No. This statement is not falsifiable.
• Yes. This statement is testable and falsifiable.

Natural disasters, such as tornadoes, are punishments for bad thoughts and behaviors.

a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

• Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
• There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
• Some types of HPV can cause cervical cancer.
• About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

• HPV causes cervical cancer.
• People should not have unprotected sex with many partners.
• People who get the vaccine will not get HPV.
• The HPV vaccine will protect people against cancer.

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—their views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

• This group is given a placebo.
• This group is deliberately infected with HPV.
• This group is given nothing.
• This group is given the HPV vaccine.
• a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
• d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

• Sex (all of the subjects will be female)
• Presence or absence of the HPV vaccine
• Presence or absence of HPV (the virus)

List three control variables other than age.

What is the dependent variable in this experiment?

• Sex (male or female)
• Rates of HPV infection
• Age (years)

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• Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
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How to Write a Research Hypothesis: Good & Bad Examples

What is a research hypothesis?

A research hypothesis is an attempt at explaining a phenomenon or the relationships between phenomena/variables in the real world. Hypotheses are sometimes called “educated guesses”, but they are in fact (or let’s say they should be) based on previous observations, existing theories, scientific evidence, and logic. A research hypothesis is also not a prediction—rather, predictions are ( should be) based on clearly formulated hypotheses. For example, “We tested the hypothesis that KLF2 knockout mice would show deficiencies in heart development” is an assumption or prediction, not a hypothesis. 

The research hypothesis at the basis of this prediction is “the product of the KLF2 gene is involved in the development of the cardiovascular system in mice”—and this hypothesis is probably (hopefully) based on a clear observation, such as that mice with low levels of Kruppel-like factor 2 (which KLF2 codes for) seem to have heart problems. From this hypothesis, you can derive the idea that a mouse in which this particular gene does not function cannot develop a normal cardiovascular system, and then make the prediction that we started with. 

What is the difference between a hypothesis and a prediction?

You might think that these are very subtle differences, and you will certainly come across many publications that do not contain an actual hypothesis or do not make these distinctions correctly. But considering that the formulation and testing of hypotheses is an integral part of the scientific method, it is good to be aware of the concepts underlying this approach. The two hallmarks of a scientific hypothesis are falsifiability (an evaluation standard that was introduced by the philosopher of science Karl Popper in 1934) and testability —if you cannot use experiments or data to decide whether an idea is true or false, then it is not a hypothesis (or at least a very bad one).

So, in a nutshell, you (1) look at existing evidence/theories, (2) come up with a hypothesis, (3) make a prediction that allows you to (4) design an experiment or data analysis to test it, and (5) come to a conclusion. Of course, not all studies have hypotheses (there is also exploratory or hypothesis-generating research), and you do not necessarily have to state your hypothesis as such in your paper. 

But for the sake of understanding the principles of the scientific method, let’s first take a closer look at the different types of hypotheses that research articles refer to and then give you a step-by-step guide for how to formulate a strong hypothesis for your own paper.

Types of Research Hypotheses

Hypotheses can be simple , which means they describe the relationship between one single independent variable (the one you observe variations in or plan to manipulate) and one single dependent variable (the one you expect to be affected by the variations/manipulation). If there are more variables on either side, you are dealing with a complex hypothesis. You can also distinguish hypotheses according to the kind of relationship between the variables you are interested in (e.g., causal or associative ). But apart from these variations, we are usually interested in what is called the “alternative hypothesis” and, in contrast to that, the “null hypothesis”. If you think these two should be listed the other way round, then you are right, logically speaking—the alternative should surely come second. However, since this is the hypothesis we (as researchers) are usually interested in, let’s start from there.

Alternative Hypothesis

If you predict a relationship between two variables in your study, then the research hypothesis that you formulate to describe that relationship is your alternative hypothesis (usually H1 in statistical terms). The goal of your hypothesis testing is thus to demonstrate that there is sufficient evidence that supports the alternative hypothesis, rather than evidence for the possibility that there is no such relationship. The alternative hypothesis is usually the research hypothesis of a study and is based on the literature, previous observations, and widely known theories. 

Null Hypothesis

The hypothesis that describes the other possible outcome, that is, that your variables are not related, is the null hypothesis ( H0 ). Based on your findings, you choose between the two hypotheses—usually that means that if your prediction was correct, you reject the null hypothesis and accept the alternative. Make sure, however, that you are not getting lost at this step of the thinking process: If your prediction is that there will be no difference or change, then you are trying to find support for the null hypothesis and reject H1. 

Directional Hypothesis

While the null hypothesis is obviously “static”, the alternative hypothesis can specify a direction for the observed relationship between variables—for example, that mice with higher expression levels of a certain protein are more active than those with lower levels. This is then called a one-tailed hypothesis. 

Another example for a directional one-tailed alternative hypothesis would be that 

H1: Attending private classes before important exams has a positive effect on performance. 

Your null hypothesis would then be that

H0: Attending private classes before important exams has no/a negative effect on performance.

Nondirectional Hypothesis

A nondirectional hypothesis does not specify the direction of the potentially observed effect, only that there is a relationship between the studied variables—this is called a two-tailed hypothesis. For instance, if you are studying a new drug that has shown some effects on pathways involved in a certain condition (e.g., anxiety) in vitro in the lab, but you can’t say for sure whether it will have the same effects in an animal model or maybe induce other/side effects that you can’t predict and potentially increase anxiety levels instead, you could state the two hypotheses like this:

H1: The only lab-tested drug (somehow) affects anxiety levels in an anxiety mouse model.

You then test this nondirectional alternative hypothesis against the null hypothesis:

H0: The only lab-tested drug has no effect on anxiety levels in an anxiety mouse model.

How to Write a Hypothesis for a Research Paper

Now that we understand the important distinctions between different kinds of research hypotheses, let’s look at a simple process of how to write a hypothesis.

Writing a Hypothesis Step:1

Ask a question, based on earlier research. Research always starts with a question, but one that takes into account what is already known about a topic or phenomenon. For example, if you are interested in whether people who have pets are happier than those who don’t, do a literature search and find out what has already been demonstrated. You will probably realize that yes, there is quite a bit of research that shows a relationship between happiness and owning a pet—and even studies that show that owning a dog is more beneficial than owning a cat ! Let’s say you are so intrigued by this finding that you wonder: 

What is it that makes dog owners even happier than cat owners? 

Let’s move on to Step 2 and find an answer to that question.

Writing a Hypothesis Step 2:

Formulate a strong hypothesis by answering your own question. Again, you don’t want to make things up, take unicorns into account, or repeat/ignore what has already been done. Looking at the dog-vs-cat papers your literature search returned, you see that most studies are based on self-report questionnaires on personality traits, mental health, and life satisfaction. What you don’t find is any data on actual (mental or physical) health measures, and no experiments. You therefore decide to make a bold claim come up with the carefully thought-through hypothesis that it’s maybe the lifestyle of the dog owners, which includes walking their dog several times per day, engaging in fun and healthy activities such as agility competitions, and taking them on trips, that gives them that extra boost in happiness. You could therefore answer your question in the following way:

Dog owners are happier than cat owners because of the dog-related activities they engage in.

Now you have to verify that your hypothesis fulfills the two requirements we introduced at the beginning of this resource article: falsifiability and testability . If it can’t be wrong and can’t be tested, it’s not a hypothesis. We are lucky, however, because yes, we can test whether owning a dog but not engaging in any of those activities leads to lower levels of happiness or well-being than owning a dog and playing and running around with them or taking them on trips.  

Writing a Hypothesis Step 3:

Make your predictions and define your variables. We have verified that we can test our hypothesis, but now we have to define all the relevant variables, design our experiment or data analysis, and make precise predictions. You could, for example, decide to study dog owners (not surprising at this point), let them fill in questionnaires about their lifestyle as well as their life satisfaction (as other studies did), and then compare two groups of active and inactive dog owners. Alternatively, if you want to go beyond the data that earlier studies produced and analyzed and directly manipulate the activity level of your dog owners to study the effect of that manipulation, you could invite them to your lab, select groups of participants with similar lifestyles, make them change their lifestyle (e.g., couch potato dog owners start agility classes, very active ones have to refrain from any fun activities for a certain period of time) and assess their happiness levels before and after the intervention. In both cases, your independent variable would be “ level of engagement in fun activities with dog” and your dependent variable would be happiness or well-being . 

Examples of a Good and Bad Hypothesis

Let’s look at a few examples of good and bad hypotheses to get you started.

Good Hypothesis Examples

Bad hypothesis examples, tips for writing a research hypothesis.

If you understood the distinction between a hypothesis and a prediction we made at the beginning of this article, then you will have no problem formulating your hypotheses and predictions correctly. To refresh your memory: We have to (1) look at existing evidence, (2) come up with a hypothesis, (3) make a prediction, and (4) design an experiment. For example, you could summarize your dog/happiness study like this:

(1) While research suggests that dog owners are happier than cat owners, there are no reports on what factors drive this difference. (2) We hypothesized that it is the fun activities that many dog owners (but very few cat owners) engage in with their pets that increases their happiness levels. (3) We thus predicted that preventing very active dog owners from engaging in such activities for some time and making very inactive dog owners take up such activities would lead to an increase and decrease in their overall self-ratings of happiness, respectively. (4) To test this, we invited dog owners into our lab, assessed their mental and emotional well-being through questionnaires, and then assigned them to an “active” and an “inactive” group, depending on… 

Note that you use “we hypothesize” only for your hypothesis, not for your experimental prediction, and “would” or “if – then” only for your prediction, not your hypothesis. A hypothesis that states that something “would” affect something else sounds as if you don’t have enough confidence to make a clear statement—in which case you can’t expect your readers to believe in your research either. Write in the present tense, don’t use modal verbs that express varying degrees of certainty (such as may, might, or could ), and remember that you are not drawing a conclusion while trying not to exaggerate but making a clear statement that you then, in a way, try to disprove . And if that happens, that is not something to fear but an important part of the scientific process.

Similarly, don’t use “we hypothesize” when you explain the implications of your research or make predictions in the conclusion section of your manuscript, since these are clearly not hypotheses in the true sense of the word. As we said earlier, you will find that many authors of academic articles do not seem to care too much about these rather subtle distinctions, but thinking very clearly about your own research will not only help you write better but also ensure that even that infamous Reviewer 2 will find fewer reasons to nitpick about your manuscript. 

Perfect Your Manuscript With Professional Editing

Now that you know how to write a strong research hypothesis for your research paper, you might be interested in our free AI proofreader , Wordvice AI, which finds and fixes errors in grammar, punctuation, and word choice in academic texts. Or if you are interested in human proofreading , check out our English editing services , including research paper editing and manuscript editing .

On the Wordvice academic resources website , you can also find many more articles and other resources that can help you with writing the other parts of your research paper , with making a research paper outline before you put everything together, or with writing an effective cover letter once you are ready to submit.

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

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Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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Hypothesis and Experimental Design

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Two important elements of The Scientific Method that will help you design your research approach more efficiently are “Generating Hypotheses” and “Designing Controlled Experiments” to test these hypotheses. A well-designed experiment that you deeply understand will save time and resources and facilitate easier data analysis/interpretation. Many people reading this may be working on a project that focuses on designing a product, or discovery research where the hypothesis it is not immediately obvious. We encourage you to read on however as the exercise of generating a hypothesis will likely help you think about the assumptions you are making in your research and the physical principles your work builds upon.     

These activities will help you …  

• Begin formulating an appropriate hypothesis related to your research.  
• Apply a systematic process for designing experiments.  

What is a Hypothesis?  

A hypothesis is an “educated guess/prediction”  or  “ proposed explanation ”  of how a system will behave based on the available evidence .  A hypothesis is a starting point for further investigation and testing   because a hypothesis makes a prediction about the behavior of a measurable outcome of an experiment.  A hypothesis should be:  

• Testable – you can design an experiment to test it  
• Falsifiable – it can be proven wrong (note it cannot be “proved”)  
• Useful – the outcome must give valuable information  

A useful hypothesis may relate to the underlying question of your research. For example:  

“We hypothesize that therapy resistant cell populations will be enriched in hypoxic microenvironments. “  

“We hypothesize that  increasing the number of boreholes simulated in 3D geological models minimizes the variation of the geological model results.”    

Some research projects do not have an obvious hypothesis to test, but the design strategy/concept chosen is based on an underlying assumption about how the system being designed works (i.e. the hypothesis). For example:  

“We hypothesize that decreasing the baking temperature of the photoresist layer will reduce thermal expansion and device cracking”   

In this case the researcher is troubleshooting poor device quality and is proposing to vary different fabrication parameters (one being baking temperature). Understanding the assumptions (working hypotheses) of why different variables might improve device quality is useful as it provides a basis to prioritize what variables to focus on first. The core goal of this research is not to test a specific hypothesis, but using the scientific method to troubleshoot a design challenge will enable the researcher to understand the parameters that control the behavior of different designs and to identify a design that is successful more efficiently.  

In all the examples above, the hypothesis helps to guide the design of a useful and interpretable experiment with appropriate controls that rule out alternative explanations of the experimental observation. Hypotheses are therefore likely essential and useful parts of all research projects.  

Suggested Activity – Create a Hypothesis for Your Research  

Estimated time: 30 mins    

• Write down the parameters you are varying or testing in your experimental system or model and how you think the behaviour of the system is going to vary with these parameters.   
• (Alternative) If your project goal is to design a device, write down the parameters you believe control whether the device will work.  
• (Alternative) If your project goal involves optimizing a process,  write down the underlying physics or chemistry controlling the process you are studying.  
• With these parameters in mind, write down the key assumption(s) you are making about how your system works. 
• Try to formulate each one of these assumptions into a hypothesis that might be useful for your research project.  If you have multiple aims each one may have a separate hypothesis. Make sure the hypothesis meets each of the three key elements above.
• Share your hypothesis with a peer or your supervisor to discuss if this is a good hypothesis – is it testable? Does it make a useful prediction? Does it capture the key underlying assumptions your research is based upon?  

Remember that writing a good research hypothesis is challenging and will take a lot of careful thought about the underlying science that governs your system.  

Designing Experiments  

Designing experiments appropriately is very important to avoid wasting resources (time!) and to ensure results can be interpreted correctly. It is often very useful to discuss the design of your planned experiments in your meetings with your supervisor to get feedback before you start doing experiments. This will also ensure you and your supervisor have a consistent understanding of experimental design and that all the appropriate controls required to interpret your data have been considered.  

The factors that must be considered when you design experiments is going to depend on your specific area of research. S ome important things to think about when designing experiments include:  

Rationale:  What is the purpose of this experiment? Is this the best experiment I can do?  Does my experiment answer  any question ?  Does this experiment help answer  the question  I am trying to ask?  What hypothesis am I trying to test?  

Will my experiment be interpretable?   What controls can I use to distinguish my results from other potential explanations? Can I add a control to distinguish between explanations? Can I add a control to further test my hypothesis?  

Is my experiment/model rigorous?   What is the  sensitivity of the method  I am using and can it measure accurately what I want to measure? What  outcomes  (metrics) will I measure and is this measurement appropriate?  How many  replicates  (technical replicates versus independent replicates) will I do?  Am I only changing the  variable  that I am testing? What am I keeping constant? What  statistical tests  do I plan to carry out and what considerations are needed? Is my statistical design appropriate (power analysis, sufficient replicates)?  

What logistics do I need to consider?  Are the equipment/resources I need available? Do I need additional training or equipment access? Are there important safety or ethical issues/permits to consider? Are pilot experiments needed to assess feasibility and what would these be? What is my planned experimental protocol and are there important timing issues to consider? What experimental outputs and parameters need to be documented throughout experiment?  

This list is not exhaustive and you should consider what is missing for your particular situation.  

Suggested Activity – Design an Experiment Using a Template  

Estimated time: 45 min  

• Explore the excel template for experimental design ( Resource 1 )   or modelling  ( Resource 2 ).  A template like this is very useful for keeping track of protocols as well as improving the reproducibility of your experiments. Note this template is simply a  starting point  to get you thinking systematically and should be adapted to best suit  your  needs.   
• Fill out the template for an experiment or modelling project you are planning to complete soon.  
• Consider how you can modify this template to be more applicable to your specific project.  
• Using the template document, explain your experimental design/model design to a peer or your supervisor. Let them ask questions to understand your design and provide feedback. Alternatively, if there is a part of your design that you are unclear about this is a great starting point for a targeted and efficient discussion with your supervisor.   
• Revise your design based on feedback.  

Activity Examples

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Research Hypothesis In Psychology: Types, & Examples

Saul Mcleod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Olivia Guy-Evans, MSc

Associate Editor for Simply Psychology

BSc (Hons) Psychology, MSc Psychology of Education

Olivia Guy-Evans is a writer and associate editor for Simply Psychology. She has previously worked in healthcare and educational sectors.

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A research hypothesis, in its plural form “hypotheses,” is a specific, testable prediction about the anticipated results of a study, established at its outset. It is a key component of the scientific method .

Hypotheses connect theory to data and guide the research process towards expanding scientific understanding

Some key points about hypotheses:

• A hypothesis expresses an expected pattern or relationship. It connects the variables under investigation.
• It is stated in clear, precise terms before any data collection or analysis occurs. This makes the hypothesis testable.
• A hypothesis must be falsifiable. It should be possible, even if unlikely in practice, to collect data that disconfirms rather than supports the hypothesis.
• Hypotheses guide research. Scientists design studies to explicitly evaluate hypotheses about how nature works.
• For a hypothesis to be valid, it must be testable against empirical evidence. The evidence can then confirm or disprove the testable predictions.
• Hypotheses are informed by background knowledge and observation, but go beyond what is already known to propose an explanation of how or why something occurs.

Predictions typically arise from a thorough knowledge of the research literature, curiosity about real-world problems or implications, and integrating this to advance theory. They build on existing literature while providing new insight.

Types of Research Hypotheses

Alternative hypothesis.

The research hypothesis is often called the alternative or experimental hypothesis in experimental research.

It typically suggests a potential relationship between two key variables: the independent variable, which the researcher manipulates, and the dependent variable, which is measured based on those changes.

The alternative hypothesis states a relationship exists between the two variables being studied (one variable affects the other).

A hypothesis is a testable statement or prediction about the relationship between two or more variables. It is a key component of the scientific method. Some key points about hypotheses:

• Important hypotheses lead to predictions that can be tested empirically. The evidence can then confirm or disprove the testable predictions.

In summary, a hypothesis is a precise, testable statement of what researchers expect to happen in a study and why. Hypotheses connect theory to data and guide the research process towards expanding scientific understanding.

An experimental hypothesis predicts what change(s) will occur in the dependent variable when the independent variable is manipulated.

It states that the results are not due to chance and are significant in supporting the theory being investigated.

The alternative hypothesis can be directional, indicating a specific direction of the effect, or non-directional, suggesting a difference without specifying its nature. It’s what researchers aim to support or demonstrate through their study.

Null Hypothesis

The null hypothesis states no relationship exists between the two variables being studied (one variable does not affect the other). There will be no changes in the dependent variable due to manipulating the independent variable.

It states results are due to chance and are not significant in supporting the idea being investigated.

The null hypothesis, positing no effect or relationship, is a foundational contrast to the research hypothesis in scientific inquiry. It establishes a baseline for statistical testing, promoting objectivity by initiating research from a neutral stance.

Many statistical methods are tailored to test the null hypothesis, determining the likelihood of observed results if no true effect exists.

This dual-hypothesis approach provides clarity, ensuring that research intentions are explicit, and fosters consistency across scientific studies, enhancing the standardization and interpretability of research outcomes.

Nondirectional Hypothesis

A non-directional hypothesis, also known as a two-tailed hypothesis, predicts that there is a difference or relationship between two variables but does not specify the direction of this relationship.

It merely indicates that a change or effect will occur without predicting which group will have higher or lower values.

For example, “There is a difference in performance between Group A and Group B” is a non-directional hypothesis.

Directional Hypothesis

A directional (one-tailed) hypothesis predicts the nature of the effect of the independent variable on the dependent variable. It predicts in which direction the change will take place. (i.e., greater, smaller, less, more)

It specifies whether one variable is greater, lesser, or different from another, rather than just indicating that there’s a difference without specifying its nature.

For example, “Exercise increases weight loss” is a directional hypothesis.

Falsifiability

The Falsification Principle, proposed by Karl Popper , is a way of demarcating science from non-science. It suggests that for a theory or hypothesis to be considered scientific, it must be testable and irrefutable.

Falsifiability emphasizes that scientific claims shouldn’t just be confirmable but should also have the potential to be proven wrong.

It means that there should exist some potential evidence or experiment that could prove the proposition false.

However many confirming instances exist for a theory, it only takes one counter observation to falsify it. For example, the hypothesis that “all swans are white,” can be falsified by observing a black swan.

For Popper, science should attempt to disprove a theory rather than attempt to continually provide evidence to support a research hypothesis.

Can a Hypothesis be Proven?

Hypotheses make probabilistic predictions. They state the expected outcome if a particular relationship exists. However, a study result supporting a hypothesis does not definitively prove it is true.

All studies have limitations. There may be unknown confounding factors or issues that limit the certainty of conclusions. Additional studies may yield different results.

In science, hypotheses can realistically only be supported with some degree of confidence, not proven. The process of science is to incrementally accumulate evidence for and against hypothesized relationships in an ongoing pursuit of better models and explanations that best fit the empirical data. But hypotheses remain open to revision and rejection if that is where the evidence leads.

• Disproving a hypothesis is definitive. Solid disconfirmatory evidence will falsify a hypothesis and require altering or discarding it based on the evidence.
• However, confirming evidence is always open to revision. Other explanations may account for the same results, and additional or contradictory evidence may emerge over time.

We can never 100% prove the alternative hypothesis. Instead, we see if we can disprove, or reject the null hypothesis.

If we reject the null hypothesis, this doesn’t mean that our alternative hypothesis is correct but does support the alternative/experimental hypothesis.

Upon analysis of the results, an alternative hypothesis can be rejected or supported, but it can never be proven to be correct. We must avoid any reference to results proving a theory as this implies 100% certainty, and there is always a chance that evidence may exist which could refute a theory.

How to Write a Hypothesis

• Identify variables . The researcher manipulates the independent variable and the dependent variable is the measured outcome.
• Operationalized the variables being investigated . Operationalization of a hypothesis refers to the process of making the variables physically measurable or testable, e.g. if you are about to study aggression, you might count the number of punches given by participants.
• Decide on a direction for your prediction . If there is evidence in the literature to support a specific effect of the independent variable on the dependent variable, write a directional (one-tailed) hypothesis. If there are limited or ambiguous findings in the literature regarding the effect of the independent variable on the dependent variable, write a non-directional (two-tailed) hypothesis.
• Make it Testable : Ensure your hypothesis can be tested through experimentation or observation. It should be possible to prove it false (principle of falsifiability).
• Clear & concise language . A strong hypothesis is concise (typically one to two sentences long), and formulated using clear and straightforward language, ensuring it’s easily understood and testable.

Consider a hypothesis many teachers might subscribe to: students work better on Monday morning than on Friday afternoon (IV=Day, DV= Standard of work).

Now, if we decide to study this by giving the same group of students a lesson on a Monday morning and a Friday afternoon and then measuring their immediate recall of the material covered in each session, we would end up with the following:

• The alternative hypothesis states that students will recall significantly more information on a Monday morning than on a Friday afternoon.
• The null hypothesis states that there will be no significant difference in the amount recalled on a Monday morning compared to a Friday afternoon. Any difference will be due to chance or confounding factors.

More Examples

• Memory : Participants exposed to classical music during study sessions will recall more items from a list than those who studied in silence.
• Social Psychology : Individuals who frequently engage in social media use will report higher levels of perceived social isolation compared to those who use it infrequently.
• Developmental Psychology : Children who engage in regular imaginative play have better problem-solving skills than those who don’t.
• Clinical Psychology : Cognitive-behavioral therapy will be more effective in reducing symptoms of anxiety over a 6-month period compared to traditional talk therapy.
• Cognitive Psychology : Individuals who multitask between various electronic devices will have shorter attention spans on focused tasks than those who single-task.
• Health Psychology : Patients who practice mindfulness meditation will experience lower levels of chronic pain compared to those who don’t meditate.
• Organizational Psychology : Employees in open-plan offices will report higher levels of stress than those in private offices.
• Behavioral Psychology : Rats rewarded with food after pressing a lever will press it more frequently than rats who receive no reward.

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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

Published on November 8, 2019 by Rebecca Bevans . Revised on June 22, 2023.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics . It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.

There are 5 main steps in hypothesis testing:

• State your research hypothesis as a null hypothesis and alternate hypothesis (H o ) and (H a  or H 1 ).
• Collect data in a way designed to test the hypothesis.
• Perform an appropriate statistical test .
• Decide whether to reject or fail to reject your null hypothesis.
• Present the findings in your results and discussion section.

Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps.

Table of contents

Step 1: state your null and alternate hypothesis, step 2: collect data, step 3: perform a statistical test, step 4: decide whether to reject or fail to reject your null hypothesis, step 5: present your findings, other interesting articles, frequently asked questions about hypothesis testing.

After developing your initial research hypothesis (the prediction that you want to investigate), it is important to restate it as a null (H o ) and alternate (H a ) hypothesis so that you can test it mathematically.

The alternate hypothesis is usually your initial hypothesis that predicts a relationship between variables. The null hypothesis is a prediction of no relationship between the variables you are interested in.

• H 0 : Men are, on average, not taller than women. H a : Men are, on average, taller than women.

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For a statistical test to be valid , it is important to perform sampling and collect data in a way that is designed to test your hypothesis. If your data are not representative, then you cannot make statistical inferences about the population you are interested in.

There are a variety of statistical tests available, but they are all based on the comparison of within-group variance (how spread out the data is within a category) versus between-group variance (how different the categories are from one another).

If the between-group variance is large enough that there is little or no overlap between groups, then your statistical test will reflect that by showing a low p -value . This means it is unlikely that the differences between these groups came about by chance.

Alternatively, if there is high within-group variance and low between-group variance, then your statistical test will reflect that with a high p -value. This means it is likely that any difference you measure between groups is due to chance.

Your choice of statistical test will be based on the type of variables and the level of measurement of your collected data .

• an estimate of the difference in average height between the two groups.
• a p -value showing how likely you are to see this difference if the null hypothesis of no difference is true.

Based on the outcome of your statistical test, you will have to decide whether to reject or fail to reject your null hypothesis.

In most cases you will use the p -value generated by your statistical test to guide your decision. And in most cases, your predetermined level of significance for rejecting the null hypothesis will be 0.05 – that is, when there is a less than 5% chance that you would see these results if the null hypothesis were true.

In some cases, researchers choose a more conservative level of significance, such as 0.01 (1%). This minimizes the risk of incorrectly rejecting the null hypothesis ( Type I error ).

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The results of hypothesis testing will be presented in the results and discussion sections of your research paper , dissertation or thesis .

In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p -value). In the discussion , you can discuss whether your initial hypothesis was supported by your results or not.

In the formal language of hypothesis testing, we talk about rejecting or failing to reject the null hypothesis. You will probably be asked to do this in your statistics assignments.

However, when presenting research results in academic papers we rarely talk this way. Instead, we go back to our alternate hypothesis (in this case, the hypothesis that men are on average taller than women) and state whether the result of our test did or did not support the alternate hypothesis.

If your null hypothesis was rejected, this result is interpreted as “supported the alternate hypothesis.”

These are superficial differences; you can see that they mean the same thing.

You might notice that we don’t say that we reject or fail to reject the alternate hypothesis . This is because hypothesis testing is not designed to prove or disprove anything. It is only designed to test whether a pattern we measure could have arisen spuriously, or by chance.

If we reject the null hypothesis based on our research (i.e., we find that it is unlikely that the pattern arose by chance), then we can say our test lends support to our hypothesis . But if the pattern does not pass our decision rule, meaning that it could have arisen by chance, then we say the test is inconsistent with our hypothesis .

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

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15 Hypothesis Examples

A hypothesis is defined as a testable prediction , and is used primarily in scientific experiments as a potential or predicted outcome that scientists attempt to prove or disprove (Atkinson et al., 2021; Tan, 2022).

In my types of hypothesis article, I outlined 13 different hypotheses, including the directional hypothesis (which makes a prediction about an effect of a treatment will be positive or negative) and the associative hypothesis (which makes a prediction about the association between two variables).

This article will dive into some interesting examples of hypotheses and examine potential ways you might test each one.

Hypothesis Examples

1. “inadequate sleep decreases memory retention”.

Field: Psychology

Type: Causal Hypothesis A causal hypothesis explores the effect of one variable on another. This example posits that a lack of adequate sleep causes decreased memory retention. In other words, if you are not getting enough sleep, your ability to remember and recall information may suffer.

How to Test:

To test this hypothesis, you might devise an experiment whereby your participants are divided into two groups: one receives an average of 8 hours of sleep per night for a week, while the other gets less than the recommended sleep amount.

During this time, all participants would daily study and recall new, specific information. You’d then measure memory retention of this information for both groups using standard memory tests and compare the results.

Should the group with less sleep have statistically significant poorer memory scores, the hypothesis would be supported.

Ensuring the integrity of the experiment requires taking into account factors such as individual health differences, stress levels, and daily nutrition.

Relevant Study: Sleep loss, learning capacity and academic performance (Curcio, Ferrara & De Gennaro, 2006)

2. “Increase in Temperature Leads to Increase in Kinetic Energy”

Field: Physics

Type: Deductive Hypothesis The deductive hypothesis applies the logic of deductive reasoning – it moves from a general premise to a more specific conclusion. This specific hypothesis assumes that as temperature increases, the kinetic energy of particles also increases – that is, when you heat something up, its particles move around more rapidly.

This hypothesis could be examined by heating a gas in a controlled environment and capturing the movement of its particles as a function of temperature.

You’d gradually increase the temperature and measure the kinetic energy of the gas particles with each increment. If the kinetic energy consistently rises with the temperature, your hypothesis gets supporting evidence.

Variables such as pressure and volume of the gas would need to be held constant to ensure validity of results.

3. “Children Raised in Bilingual Homes Develop Better Cognitive Skills”

Field: Psychology/Linguistics

Type: Comparative Hypothesis The comparative hypothesis posits a difference between two or more groups based on certain variables. In this context, you might propose that children raised in bilingual homes have superior cognitive skills compared to those raised in monolingual homes.

Testing this hypothesis could involve identifying two groups of children: those raised in bilingual homes, and those raised in monolingual homes.

Cognitive skills in both groups would be evaluated using a standard cognitive ability test at different stages of development. The examination would be repeated over a significant time period for consistency.

If the group raised in bilingual homes persistently scores higher than the other, the hypothesis would thereby be supported.

The challenge for the researcher would be controlling for other variables that could impact cognitive development, such as socio-economic status, education level of parents, and parenting styles.

Relevant Study: The cognitive benefits of being bilingual (Marian & Shook, 2012)

4. “High-Fiber Diet Leads to Lower Incidences of Cardiovascular Diseases”

Field: Medicine/Nutrition

Type: Alternative Hypothesis The alternative hypothesis suggests an alternative to a null hypothesis. In this context, the implied null hypothesis could be that diet has no effect on cardiovascular health, which the alternative hypothesis contradicts by suggesting that a high-fiber diet leads to fewer instances of cardiovascular diseases.

To test this hypothesis, a longitudinal study could be conducted on two groups of participants; one adheres to a high-fiber diet, while the other follows a diet low in fiber.

After a fixed period, the cardiovascular health of participants in both groups could be analyzed and compared. If the group following a high-fiber diet has a lower number of recorded cases of cardiovascular diseases, it would provide evidence supporting the hypothesis.

Control measures should be implemented to exclude the influence of other lifestyle and genetic factors that contribute to cardiovascular health.

Relevant Study: Dietary fiber, inflammation, and cardiovascular disease (King, 2005)

5. “Gravity Influences the Directional Growth of Plants”

Field: Agronomy / Botany

Type: Explanatory Hypothesis An explanatory hypothesis attempts to explain a phenomenon. In this case, the hypothesis proposes that gravity affects how plants direct their growth – both above-ground (toward sunlight) and below-ground (towards water and other resources).

The testing could be conducted by growing plants in a rotating cylinder to create artificial gravity.

Observations on the direction of growth, over a specified period, can provide insights into the influencing factors. If plants consistently direct their growth in a manner that indicates the influence of gravitational pull, the hypothesis is substantiated.

It is crucial to ensure that other growth-influencing factors, such as light and water, are uniformly distributed so that only gravity influences the directional growth.

6. “The Implementation of Gamified Learning Improves Students’ Motivation”

Field: Education

Type: Relational Hypothesis The relational hypothesis describes the relation between two variables. Here, the hypothesis is that the implementation of gamified learning has a positive effect on the motivation of students.

To validate this proposition, two sets of classes could be compared: one that implements a learning approach with game-based elements, and another that follows a traditional learning approach.

The students’ motivation levels could be gauged by monitoring their engagement, performance, and feedback over a considerable timeframe.

If the students engaged in the gamified learning context present higher levels of motivation and achievement, the hypothesis would be supported.

Control measures ought to be put into place to account for individual differences, including prior knowledge and attitudes towards learning.

Relevant Study: Does educational gamification improve students’ motivation? (Chapman & Rich, 2018)

7. “Mathematics Anxiety Negatively Affects Performance”

Field: Educational Psychology

Type: Research Hypothesis The research hypothesis involves making a prediction that will be tested. In this case, the hypothesis proposes that a student’s anxiety about math can negatively influence their performance in math-related tasks.

To assess this hypothesis, researchers must first measure the mathematics anxiety levels of a sample of students using a validated instrument, such as the Mathematics Anxiety Rating Scale.

Then, the students’ performance in mathematics would be evaluated through standard testing. If there’s a negative correlation between the levels of math anxiety and math performance (meaning as anxiety increases, performance decreases), the hypothesis would be supported.

It would be crucial to control for relevant factors such as overall academic performance and previous mathematical achievement.

8. “Disruption of Natural Sleep Cycle Impairs Worker Productivity”

Field: Organizational Psychology

Type: Operational Hypothesis The operational hypothesis involves defining the variables in measurable terms. In this example, the hypothesis posits that disrupting the natural sleep cycle, for instance through shift work or irregular working hours, can lessen productivity among workers.

To test this hypothesis, you could collect data from workers who maintain regular working hours and those with irregular schedules.

Measuring productivity could involve examining the worker’s ability to complete tasks, the quality of their work, and their efficiency.

If workers with interrupted sleep cycles demonstrate lower productivity compared to those with regular sleep patterns, it would lend support to the hypothesis.

Consideration should be given to potential confounding variables such as job type, worker age, and overall health.

9. “Regular Physical Activity Reduces the Risk of Depression”

Field: Health Psychology

Type: Predictive Hypothesis A predictive hypothesis involves making a prediction about the outcome of a study based on the observed relationship between variables. In this case, it is hypothesized that individuals who engage in regular physical activity are less likely to suffer from depression.

Longitudinal studies would suit to test this hypothesis, tracking participants’ levels of physical activity and their mental health status over time.

The level of physical activity could be self-reported or monitored, while mental health status could be assessed using standard diagnostic tools or surveys.

If data analysis shows that participants maintaining regular physical activity have a lower incidence of depression, this would endorse the hypothesis.

However, care should be taken to control other lifestyle and behavioral factors that could intervene with the results.

Relevant Study: Regular physical exercise and its association with depression (Kim, 2022)

10. “Regular Meditation Enhances Emotional Stability”

Type: Empirical Hypothesis In the empirical hypothesis, predictions are based on amassed empirical evidence . This particular hypothesis theorizes that frequent meditation leads to improved emotional stability, resonating with numerous studies linking meditation to a variety of psychological benefits.

Earlier studies reported some correlations, but to test this hypothesis directly, you’d organize an experiment where one group meditates regularly over a set period while a control group doesn’t.

Both groups’ emotional stability levels would be measured at the start and end of the experiment using a validated emotional stability assessment.

If regular meditators display noticeable improvements in emotional stability compared to the control group, the hypothesis gains credit.

You’d have to ensure a similar emotional baseline for all participants at the start to avoid skewed results.

11. “Children Exposed to Reading at an Early Age Show Superior Academic Progress”

Type: Directional Hypothesis The directional hypothesis predicts the direction of an expected relationship between variables. Here, the hypothesis anticipates that early exposure to reading positively affects a child’s academic advancement.

A longitudinal study tracking children’s reading habits from an early age and their consequent academic performance could validate this hypothesis.

Parents could report their children’s exposure to reading at home, while standardized school exam results would provide a measure of academic achievement.

If the children exposed to early reading consistently perform better acadically, it gives weight to the hypothesis.

However, it would be important to control for variables that might impact academic performance, such as socioeconomic background, parental education level, and school quality.

12. “Adopting Energy-efficient Technologies Reduces Carbon Footprint of Industries”

Field: Environmental Science

Type: Descriptive Hypothesis A descriptive hypothesis predicts the existence of an association or pattern related to variables. In this scenario, the hypothesis suggests that industries adopting energy-efficient technologies will resultantly show a reduced carbon footprint.

Global industries making use of energy-efficient technologies could track their carbon emissions over time. At the same time, others not implementing such technologies continue their regular tracking.

After a defined time, the carbon emission data of both groups could be compared. If industries that adopted energy-efficient technologies demonstrate a notable reduction in their carbon footprints, the hypothesis would hold strong.

In the experiment, you would exclude variations brought by factors such as industry type, size, and location.

13. “Reduced Screen Time Improves Sleep Quality”

Type: Simple Hypothesis The simple hypothesis is a prediction about the relationship between two variables, excluding any other variables from consideration. This example posits that by reducing time spent on devices like smartphones and computers, an individual should experience improved sleep quality.

A sample group would need to reduce their daily screen time for a pre-determined period. Sleep quality before and after the reduction could be measured using self-report sleep diaries and objective measures like actigraphy, monitoring movement and wakefulness during sleep.

If the data shows that sleep quality improved post the screen time reduction, the hypothesis would be validated.

Other aspects affecting sleep quality, like caffeine intake, should be controlled during the experiment.

Relevant Study: Screen time use impacts low‐income preschool children’s sleep quality, tiredness, and ability to fall asleep (Waller et al., 2021)

14. Engaging in Brain-Training Games Improves Cognitive Functioning in Elderly

Field: Gerontology

Type: Inductive Hypothesis Inductive hypotheses are based on observations leading to broader generalizations and theories. In this context, the hypothesis deduces from observed instances that engaging in brain-training games can help improve cognitive functioning in the elderly.

A longitudinal study could be conducted where an experimental group of elderly people partakes in regular brain-training games.

Their cognitive functioning could be assessed at the start of the study and at regular intervals using standard neuropsychological tests.

If the group engaging in brain-training games shows better cognitive functioning scores over time compared to a control group not playing these games, the hypothesis would be supported.

15. Farming Practices Influence Soil Erosion Rates

Type: Null Hypothesis A null hypothesis is a negative statement assuming no relationship or difference between variables. The hypothesis in this context asserts there’s no effect of different farming practices on the rates of soil erosion.

Comparing soil erosion rates in areas with different farming practices over a considerable timeframe could help test this hypothesis.

If, statistically, the farming practices do not lead to differences in soil erosion rates, the null hypothesis is accepted.

However, if marked variation appears, the null hypothesis is rejected, meaning farming practices do influence soil erosion rates. It would be crucial to control for external factors like weather, soil type, and natural vegetation.

The variety of hypotheses mentioned above underscores the diversity of research constructs inherent in different fields, each with its unique purpose and way of testing.

While researchers may develop hypotheses primarily as tools to define and narrow the focus of the study, these hypotheses also serve as valuable guiding forces for the data collection and analysis procedures, making the research process more efficient and direction-focused.

Hypotheses serve as a compass for any form of academic research. The diverse examples provided, from Psychology to Educational Studies, Environmental Science to Gerontology, clearly demonstrate how certain hypotheses suit specific fields more aptly than others.

It is important to underline that although these varied hypotheses differ in their structure and methods of testing, each endorses the fundamental value of empiricism in research. Evidence-based decision making remains at the heart of scholarly inquiry, regardless of the research field, thus aligning all hypotheses to the core purpose of scientific investigation.

Testing hypotheses is an essential part of the scientific method . By doing so, researchers can either confirm their predictions, giving further validity to an existing theory, or they might uncover new insights that could potentially shift the field’s understanding of a particular phenomenon. In either case, hypotheses serve as the stepping stones for scientific exploration and discovery.

Atkinson, P., Delamont, S., Cernat, A., Sakshaug, J. W., & Williams, R. A. (2021).  SAGE research methods foundations . SAGE Publications Ltd.

Curcio, G., Ferrara, M., & De Gennaro, L. (2006). Sleep loss, learning capacity and academic performance.  Sleep medicine reviews ,  10 (5), 323-337.

Kim, J. H. (2022). Regular physical exercise and its association with depression: A population-based study short title: Exercise and depression.  Psychiatry Research ,  309 , 114406.

King, D. E. (2005). Dietary fiber, inflammation, and cardiovascular disease.  Molecular nutrition & food research ,  49 (6), 594-600.

Marian, V., & Shook, A. (2012, September). The cognitive benefits of being bilingual. In Cerebrum: the Dana forum on brain science (Vol. 2012). Dana Foundation.

Tan, W. C. K. (2022). Research Methods: A Practical Guide For Students And Researchers (Second Edition) . World Scientific Publishing Company.

Waller, N. A., Zhang, N., Cocci, A. H., D’Agostino, C., Wesolek‐Greenson, S., Wheelock, K., … & Resnicow, K. (2021). Screen time use impacts low‐income preschool children’s sleep quality, tiredness, and ability to fall asleep. Child: care, health and development, 47 (5), 618-626.

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Dr. Chris Drew is the founder of the Helpful Professor. He holds a PhD in education and has published over 20 articles in scholarly journals. He is the former editor of the Journal of Learning Development in Higher Education. [Image Descriptor: Photo of Chris]

• Chris Drew (PhD) https://helpfulprofessor.com/author/chris-drew-phd/ 5 Top Tips for Succeeding at University
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What Is a Hypothesis? (Science)

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A hypothesis (plural hypotheses) is a proposed explanation for an observation. The definition depends on the subject.

In science, a hypothesis is part of the scientific method. It is a prediction or explanation that is tested by an experiment. Observations and experiments may disprove a scientific hypothesis, but can never entirely prove one.

In the study of logic, a hypothesis is an if-then proposition, typically written in the form, "If X , then Y ."

In common usage, a hypothesis is simply a proposed explanation or prediction, which may or may not be tested.

Writing a Hypothesis

Most scientific hypotheses are proposed in the if-then format because it's easy to design an experiment to see whether or not a cause and effect relationship exists between the independent variable and the dependent variable . The hypothesis is written as a prediction of the outcome of the experiment.

• Null Hypothesis and Alternative Hypothesis

Statistically, it's easier to show there is no relationship between two variables than to support their connection. So, scientists often propose the null hypothesis . The null hypothesis assumes changing the independent variable will have no effect on the dependent variable.

In contrast, the alternative hypothesis suggests changing the independent variable will have an effect on the dependent variable. Designing an experiment to test this hypothesis can be trickier because there are many ways to state an alternative hypothesis.

For example, consider a possible relationship between getting a good night's sleep and getting good grades. The null hypothesis might be stated: "The number of hours of sleep students get is unrelated to their grades" or "There is no correlation between hours of sleep and grades."

An experiment to test this hypothesis might involve collecting data, recording average hours of sleep for each student and grades. If a student who gets eight hours of sleep generally does better than students who get four hours of sleep or 10 hours of sleep, the hypothesis might be rejected.

But the alternative hypothesis is harder to propose and test. The most general statement would be: "The amount of sleep students get affects their grades." The hypothesis might also be stated as "If you get more sleep, your grades will improve" or "Students who get nine hours of sleep have better grades than those who get more or less sleep."

In an experiment, you can collect the same data, but the statistical analysis is less likely to give you a high confidence limit.

Usually, a scientist starts out with the null hypothesis. From there, it may be possible to propose and test an alternative hypothesis, to narrow down the relationship between the variables.

Example of a Hypothesis

Examples of a hypothesis include:

• If you drop a rock and a feather, (then) they will fall at the same rate.
• Plants need sunlight in order to live. (if sunlight, then life)
• Eating sugar gives you energy. (if sugar, then energy)
• White, Jay D.  Research in Public Administration . Conn., 1998.
• Schick, Theodore, and Lewis Vaughn.  How to Think about Weird Things: Critical Thinking for a New Age . McGraw-Hill Higher Education, 2002.
• Null Hypothesis Definition and Examples
• Definition of a Hypothesis
• What Are the Elements of a Good Hypothesis?
• Six Steps of the Scientific Method
• What Are Examples of a Hypothesis?
• Understanding Simple vs Controlled Experiments
• Scientific Method Flow Chart
• Scientific Method Vocabulary Terms
• What Is a Testable Hypothesis?
• Null Hypothesis Examples
• What 'Fail to Reject' Means in a Hypothesis Test
• How To Design a Science Fair Experiment
• What Is an Experiment? Definition and Design
• Hypothesis Test for the Difference of Two Population Proportions
• How to Conduct a Hypothesis Test

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Biology library

Course: biology library   >   unit 1.

• The scientific method

Controlled experiments

• The scientific method and experimental design

Introduction

How are hypotheses tested.

• One pot of seeds gets watered every afternoon.
• The other pot of seeds doesn't get any water at all.

Control and experimental groups

Independent and dependent variables, independent variables, dependent variables, variability and repetition, controlled experiment case study: co 2 ‍   and coral bleaching.

• What your control and experimental groups would be
• What your independent and dependent variables would be
• What results you would predict in each group

Experimental setup

• Some corals were grown in tanks of normal seawater, which is not very acidic ( pH ‍   around 8.2 ‍   ). The corals in these tanks served as the control group .
• Other corals were grown in tanks of seawater that were more acidic than usual due to addition of CO 2 ‍   . One set of tanks was medium-acidity ( pH ‍   about 7.9 ‍   ), while another set was high-acidity ( pH ‍   about 7.65 ‍   ). Both the medium-acidity and high-acidity groups were experimental groups .
• In this experiment, the independent variable was the acidity ( pH ‍   ) of the seawater. The dependent variable was the degree of bleaching of the corals.
• The researchers used a large sample size and repeated their experiment. Each tank held 5 ‍   fragments of coral, and there were 5 ‍   identical tanks for each group (control, medium-acidity, and high-acidity). Experimental setup to test effects of water acidity on coral bleaching. Control group: Coral fragments are placed in a tank of normal seawater (pH 8.2). Experimental group 1: Coral fragments are placed in a tank of slightly acidified seawater (pH 7.9). Experimental group 2: Coral fragments are placed in a tank of more strongly acidified seawater (pH 7.65). The water acidity is the independent variable. 8 weeks are allowed to pass for each of the tanks... Control group: Corals are about 10% bleached on average. Experimental group 1 (medium acidity): Corals are about 20% bleached on average. Experimental group 2 (higher acidity): Corals are about 40% bleached on average. Degree of coral bleaching is the dependent variable. Note: None of these tanks was "acidic" on an absolute scale. That is, the pH ‍   values were all above the neutral pH ‍   of 7.0 ‍   . However, the two groups of experimental tanks were moderately and highly acidic to the corals , that is, relative to their natural habitat of plain seawater.

Analyzing the results

Non-experimental hypothesis tests, case study: coral bleaching and temperature, attribution:, works cited:.

• Hoegh-Guldberg, O. (1999). Climate change, coral bleaching, and the future of the world's coral reefs. Mar. Freshwater Res. , 50 , 839-866. Retrieved from www.reef.edu.au/climate/Hoegh-Guldberg%201999.pdf.
• Anthony, K. R. N., Kline, D. I., Diaz-Pulido, G., Dove, S., and Hoegh-Guldberg, O. (2008). Ocean acidification causes bleaching and productivity loss in coral reef builders. PNAS , 105 (45), 17442-17446. http://dx.doi.org/10.1073/pnas.0804478105 .
• University of California Museum of Paleontology. (2016). Misconceptions about science. In Understanding science . Retrieved from http://undsci.berkeley.edu/teaching/misconceptions.php .
• Hoegh-Guldberg, O. and Smith, G. J. (1989). The effect of sudden changes in temperature, light and salinity on the density and export of zooxanthellae from the reef corals Stylophora pistillata (Esper, 1797) and Seriatopora hystrix (Dana, 1846). J. Exp. Mar. Biol. Ecol. , 129 , 279-303. Retrieved from http://www.reef.edu.au/ohg/res-pic/HG%20papers/HG%20and%20Smith%201989%20BLEACH.pdf .

Experiment Definition in Science – What Is a Science Experiment?

In science, an experiment is simply a test of a hypothesis in the scientific method . It is a controlled examination of cause and effect. Here is a look at what a science experiment is (and is not), the key factors in an experiment, examples, and types of experiments.

Experiment Definition in Science

By definition, an experiment is a procedure that tests a hypothesis. A hypothesis, in turn, is a prediction of cause and effect or the predicted outcome of changing one factor of a situation. Both the hypothesis and experiment are components of the scientific method. The steps of the scientific method are:

• Make observations.
• Ask a question or identify a problem.
• State a hypothesis.
• Perform an experiment that tests the hypothesis.
• Based on the results of the experiment, either accept or reject the hypothesis.
• Draw conclusions and report the outcome of the experiment.

Key Parts of an Experiment

The two key parts of an experiment are the independent and dependent variables. The independent variable is the one factor that you control or change in an experiment. The dependent variable is the factor that you measure that responds to the independent variable. An experiment often includes other types of variables , but at its heart, it’s all about the relationship between the independent and dependent variable.

Examples of Experiments

Fertilizer and plant size.

For example, you think a certain fertilizer helps plants grow better. You’ve watched your plants grow and they seem to do better when they have the fertilizer compared to when they don’t. But, observations are only the beginning of science. So, you state a hypothesis: Adding fertilizer increases plant size. Note, you could have stated the hypothesis in different ways. Maybe you think the fertilizer increases plant mass or fruit production, for example. However you state the hypothesis, it includes both the independent and dependent variables. In this case, the independent variable is the presence or absence of fertilizer. The dependent variable is the response to the independent variable, which is the size of the plants.

Now that you have a hypothesis, the next step is designing an experiment that tests it. Experimental design is very important because the way you conduct an experiment influences its outcome. For example, if you use too small of an amount of fertilizer you may see no effect from the treatment. Or, if you dump an entire container of fertilizer on a plant you could kill it! So, recording the steps of the experiment help you judge the outcome of the experiment and aid others who come after you and examine your work. Other factors that might influence your results might include the species of plant and duration of the treatment. Record any conditions that might affect the outcome. Ideally, you want the only difference between your two groups of plants to be whether or not they receive fertilizer. Then, measure the height of the plants and see if there is a difference between the two groups.

Salt and Cookies

You don’t need a lab for an experiment. For example, consider a baking experiment. Let’s say you like the flavor of salt in your cookies, but you’re pretty sure the batch you made using extra salt fell a bit flat. If you double the amount of salt in a recipe, will it affect their size? Here, the independent variable is the amount of salt in the recipe and the dependent variable is cookie size.

Test this hypothesis with an experiment. Bake cookies using the normal recipe (your control group ) and bake some using twice the salt (the experimental group). Make sure it’s the exact same recipe. Bake the cookies at the same temperature and for the same time. Only change the amount of salt in the recipe. Then measure the height or diameter of the cookies and decide whether to accept or reject the hypothesis.

Examples of Things That Are Not Experiments

Based on the examples of experiments, you should see what is not an experiment:

• Making observations does not constitute an experiment. Initial observations often lead to an experiment, but are not a substitute for one.
• Making a model is not an experiment.
• Neither is making a poster.
• Just trying something to see what happens is not an experiment. You need a hypothesis or prediction about the outcome.
• Changing a lot of things at once isn’t an experiment. You only have one independent and one dependent variable. However, in an experiment, you might suspect the independent variable has an effect on a separate. So, you design a new experiment to test this.

Types of Experiments

There are three main types of experiments: controlled experiments, natural experiments, and field experiments,

• Controlled experiment : A controlled experiment compares two groups of samples that differ only in independent variable. For example, a drug trial compares the effect of a group taking a placebo (control group) against those getting the drug (the treatment group). Experiments in a lab or home generally are controlled experiments
• Natural experiment : Another name for a natural experiment is a quasi-experiment. In this type of experiment, the researcher does not directly control the independent variable, plus there may be other variables at play. Here, the goal is establishing a correlation between the independent and dependent variable. For example, in the formation of new elements a scientist hypothesizes that a certain collision between particles creates a new atom. But, other outcomes may be possible. Or, perhaps only decay products are observed that indicate the element, and not the new atom itself. Many fields of science rely on natural experiments, since controlled experiments aren’t always possible.
• Field experiment : While a controlled experiments takes place in a lab or other controlled setting, a field experiment occurs in a natural setting. Some phenomena cannot be readily studied in a lab or else the setting exerts an influence that affects the results. So, a field experiment may have higher validity. However, since the setting is not controlled, it is also subject to external factors and potential contamination. For example, if you study whether a certain plumage color affects bird mate selection, a field experiment in a natural environment eliminates the stressors of an artificial environment. Yet, other factors that could be controlled in a lab may influence results. For example, nutrition and health are controlled in a lab, but not in the field.
• Bailey, R.A. (2008). Design of Comparative Experiments . Cambridge: Cambridge University Press. ISBN 9780521683579.
• di Francia, G. Toraldo (1981). The Investigation of the Physical World . Cambridge University Press. ISBN 0-521-29925-X.
• Hinkelmann, Klaus; Kempthorne, Oscar (2008). Design and Analysis of Experiments. Volume I: Introduction to Experimental Design (2nd ed.). Wiley. ISBN 978-0-471-72756-9.
• Holland, Paul W. (December 1986). “Statistics and Causal Inference”.  Journal of the American Statistical Association . 81 (396): 945–960. doi: 10.2307/2289064
• Stohr-Hunt, Patricia (1996). “An Analysis of Frequency of Hands-on Experience and Science Achievement”. Journal of Research in Science Teaching . 33 (1): 101–109. doi: 10.1002/(SICI)1098-2736(199601)33:1<101::AID-TEA6>3.0.CO;2-Z

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Original research article, learning scientific observation with worked examples in a digital learning environment.

• 1 Department Educational Sciences, Chair for Formal and Informal Learning, Technical University Munich School of Social Sciences and Technology, Munich, Germany
• 2 Aquatic Systems Biology Unit, TUM School of Life Sciences, Technical University of Munich, Freising, Germany

Science education often aims to increase learners’ acquisition of fundamental principles, such as learning the basic steps of scientific methods. Worked examples (WE) have proven particularly useful for supporting the development of such cognitive schemas and successive actions in order to avoid using up more cognitive resources than are necessary. Therefore, we investigated the extent to which heuristic WE are beneficial for supporting the acquisition of a basic scientific methodological skill—conducting scientific observation. The current study has a one-factorial, quasi-experimental, comparative research design and was conducted as a field experiment. Sixty two students of a German University learned about scientific observation steps during a course on applying a fluvial audit, in which several sections of a river were classified based on specific morphological characteristics. In the two experimental groups scientific observation was supported either via faded WE or via non-faded WE both presented as short videos. The control group did not receive support via WE. We assessed factual and applied knowledge acquisition regarding scientific observation, motivational aspects and cognitive load. The results suggest that WE promoted knowledge application: Learners from both experimental groups were able to perform the individual steps of scientific observation more accurately. Fading of WE did not show any additional advantage compared to the non-faded version in this regard. Furthermore, the descriptive results reveal higher motivation and reduced extraneous cognitive load within the experimental groups, but none of these differences were statistically significant. Our findings add to existing evidence that WE may be useful to establish scientific competences.

1 Introduction

Learning in science education frequently involves the acquisition of basic principles or generalities, whether of domain-specific topics (e.g., applying a mathematical multiplication rule) or of rather universal scientific methodologies (e.g., performing the steps of scientific observation) ( Lunetta et al., 2007 ). Previous research has shown that worked examples (WE) can be considered particularly useful for developing such cognitive schemata during learning to avoid using more cognitive resources than necessary for learning successive actions ( Renkl et al., 2004 ; Renkl, 2017 ). WE consist of the presentation of a problem, consecutive solution steps and the solution itself. This is especially advantageous in initial cognitive skill acquisition, i.e., for novice learners with low prior knowledge ( Kalyuga et al., 2001 ). With growing knowledge, fading WE can lead from example-based learning to independent problem-solving ( Renkl et al., 2002 ). Preliminary work has shown the advantage of WE in specific STEM domains like mathematics ( Booth et al., 2015 ; Barbieri et al., 2021 ), but less studies have investigated their impact on the acquisition of basic scientific competencies that involve heuristic problem-solving processes (scientific argumentation, Schworm and Renkl, 2007 ; Hefter et al., 2014 ; Koenen et al., 2017 ). In the realm of natural sciences, various basic scientific methodologies are employed to acquire knowledge, such as experimentation or scientific observation ( Wellnitz and Mayer, 2013 ). During the pursuit of knowledge through scientific inquiry activities, learners may encounter several challenges and difficulties. Similar to the hurdles faced in experimentation, where understanding the criteria for appropriate experimental design, including the development, measurement, and evaluation of results, is crucial ( Sirum and Humburg, 2011 ; Brownell et al., 2014 ; Dasgupta et al., 2014 ; Deane et al., 2014 ), scientific observation additionally presents its own set of issues. In scientific observation, e.g., the acquisition of new insights may be somewhat incidental due to spontaneous and uncoordinated observations ( Jensen, 2014 ). To address these challenges, it is crucial to provide instructional support, including the use of WE, particularly when observations are carried out in a more self-directed manner.

For this reason, the aim of the present study was to determine the usefulness of digitally presented WE to support the acquisition of a basic scientific methodological skill—conducting scientific observations—using a digital learning environment. In this regard, this study examined the effects of different forms of digitally presented WE (non-faded vs. faded) on students’ cognitive and motivational outcomes and compared them to a control group without WE. Furthermore, the combined perspective of factual and applied knowledge, as well as motivational and cognitive aspects, represent further value added to the study.

2 Theoretical background

2.1 worked examples.

WE have been commonly used in the fields of STEM education (science, technology, engineering, and mathematics) ( Booth et al., 2015 ). They consist of a problem statement, the steps to solve the problem, and the solution itself ( Atkinson et al., 2000 ; Renkl et al., 2002 ; Renkl, 2014 ). The success of WE can be explained by their impact on cognitive load (CL) during learning, based on assumptions from Cognitive Load Theory ( Sweller, 2006 ).

Learning with WE is considered time-efficient, effective, and superior to problem-based learning (presentation of the problem without demonstration of solution steps) when it comes to knowledge acquisition and transfer (WE-effect, Atkinson et al., 2000 ; Van Gog et al., 2011 ). Especially WE can help by reducing the extraneous load (presentation and design of the learning material) and, in turn, can lead to an increase in germane load (effort of the learner to understand the learning material) ( Paas et al., 2003 ; Renkl, 2014 ). With regard to intrinsic load (difficulty and complexity of the learning material), it is still controversially discussed if it can be altered by instructional design, e.g., WE ( Gerjets et al., 2004 ). WE have a positive effect on learning and knowledge transfer, especially for novices, as the step-by-step presentation of the solution requires less extraneous mental effort compared to problem-based learning ( Sweller et al., 1998 ; Atkinson et al., 2000 ; Bokosmaty et al., 2015 ). With growing knowledge, WE can lose their advantages (due to the expertise-reversal effect), and scaffolding learning via faded WE might be more successful for knowledge gain and transfer ( Renkl, 2014 ). Faded WE are similar to complete WE, but fade out solution steps as knowledge and competencies grow. Faded WE enhance near-knowledge transfer and reduce errors compared to non-faded WE ( Renkl et al., 2000 ).

In addition, the reduction of intrinsic and extraneous CL by WE also has an impact on learner motivation, such as interest ( Van Gog and Paas, 2006 ). Um et al. (2012) showed that there is a strong positive correlation between germane CL and the motivational aspects of learning, like satisfaction and emotion. Gupta (2019) mentions a positive correlation between CL and interest. Van Harsel et al. (2019) found that WE positively affect learning motivation, while no such effect was found for problem-solving. Furthermore, learning with WE increases the learners’ belief in their competence in completing a task. In addition, fading WE can lead to higher motivation for more experienced learners, while non-faded WE can be particularly motivating for learners without prior knowledge ( Paas et al., 2005 ). In general, fundamental motivational aspects during the learning process, such as situational interest ( Lewalter and Knogler, 2014 ) or motivation-relevant experiences, like basic needs, are influenced by learning environments. At the same time, their use also depends on motivational characteristics of the learning process, such as self-determined motivation ( Deci and Ryan, 2012 ). Therefore, we assume that learning with WE as a relevant component of a learning environment might also influence situational interest and basic needs.

2.1.1 Presentation of worked examples

WE are frequently used in digital learning scenarios ( Renkl, 2014 ). When designing WE, the application via digital learning media can be helpful, as their content can be presented in different ways (video, audio, text, and images), tailored to the needs of the learners, so that individual use is possible according to their own prior knowledge or learning pace ( Mayer, 2001 ). Also, digital media can present relevant information in a timely, motivating, appealing and individualized way and support learning in an effective and needs-oriented way ( Mayer, 2001 ). The advantages of using digital media in designing WE have already been shown in previous studies. Dart et al. (2020) presented WE as short videos (WEV). They report that the use of WEV leads to increased student satisfaction and more positive attitudes. Approximately 90% of the students indicated an active learning approach when learning with the WEV. Furthermore, the results show that students improved their content knowledge through WEV and that they found WEV useful for other courses as well.

Another study ( Kay and Edwards, 2012 ) presented WE as video podcasts. Here, the advantages of WE regarding self-determined learning in terms of learning location, learning time, and learning speed were shown. Learning performance improved significantly after use. The step-by-step, easy-to-understand explanations, the diagrams, and the ability to determine the learning pace by oneself were seen as beneficial.

Multimedia WE can also be enhanced with self-explanation prompts ( Berthold et al., 2009 ). Learning from WE with self-explanation prompts was shown to be superior to other learning methods, such as hypertext learning and observational learning.

In addition to presenting WE in different medial ways, WE can also comprise different content domains.

2.1.2 Content and context of worked examples

Regarding the content of WE, algorithmic and heuristic WE, as well as single-content and double-content WE, can be distinguished ( Reiss et al., 2008 ; Koenen et al., 2017 ; Renkl, 2017 ). Algorithmic WE are traditionally used in the very structured mathematical–physical field. Here, an algorithm with very specific solution steps is to learn, for example, in probability calculation ( Koenen et al., 2017 ). In this study, however, we focus on heuristic double-content WE. Heuristic WE in science education comprise fundamental scientific working methods, e.g., conducting experiments ( Koenen et al., 2017 ). Furthermore, double-content WE contain two learning domains that are relevant for the learning process: (1) the learning domain describes the primarily to be learned abstract process or concept, e.g., scientific methodologies like observation (see section 2.2), while (2) the exemplifying domain consists of the content that is necessary to teach this process or concept, e.g., mapping of river structure ( Renkl et al., 2009 ).

Depending on the WE content to be learned, it may be necessary for learning to take place in different settings. This can be in a formal or informal learning setting or a non-formal field setting. In this study, the focus is on learning scientific observation (learning domain) through river structure mapping (exemplary domain), which takes place with the support of digital media in a formal (university) setting, but in an informal context (nature).

2.2 Scientific observation

Scientific observation is fundamental to all scientific activities and disciplines ( Kohlhauf et al., 2011 ). Scientific observation must be clearly distinguished from everyday observation, where observation is purely a matter of noticing and describing specific characteristics ( Chinn and Malhotra, 2001 ). In contrast to this everyday observation, scientific observation as a method of knowledge acquisition can be described as a rather complex activity, defined as the theory-based, systematic and selective perception of concrete systems and processes without any fundamental manipulation ( Wellnitz and Mayer, 2013 ). Wellnitz and Mayer (2013) described the scientific observation process via six steps: (1) formulation of the research question (s), (2) deduction of the null hypothesis and the alternative hypothesis, (3) planning of the research design, (4) conducting the observation, (5) analyzing the data, and (6) answering the research question(s) on this basis. Only through reliable and qualified observation, valid data can be obtained that provide solid scientific evidence ( Wellnitz and Mayer, 2013 ).

Since observation activities are not trivial and learners often observe without generating new knowledge or connecting their observations to scientific explanations and thoughts, it is important to provide support at the related cognitive level, so that observation activities can be conducted in a structured way according to pre-defined criteria ( Ford, 2005 ; Eberbach and Crowley, 2009 ). Especially during field-learning experiences, scientific observation is often spontaneous and uncoordinated, whereby random discoveries result in knowledge gain ( Jensen, 2014 ).

To promote successful observing in rather unstructured settings like field trips, instructional support for the observation process seems useful. To guide observation activities, digitally presented WE seem to be an appropriate way to introduce learners to the individual steps of scientific observation using concrete examples.

2.3 Research questions and hypothesis

The present study investigates the effect of digitally presented double-content WE that supports the mapping of a small Bavarian river by demonstrating the steps of scientific observation. In this analysis, we focus on the learning domain of the WE and do not investigate the exemplifying domain in detail. Distinct ways of integrating WE in the digital learning environment (faded WE vs. non-faded WE) are compared with each other and with a control group (no WE). The aim is to examine to what extent differences between those conditions exist with regard to (RQ1) learners’ competence acquisition [acquisition of factual knowledge about the scientific observation method (quantitative data) and practical application of the scientific observation method (quantified qualitative data)], (RQ2) learners’ motivation (situational interest and basic needs), and (RQ3) CL. It is assumed that (Hypothesis 1), the integration of WE (faded and non-faded) leads to significantly higher competence acquisition (factual and applied knowledge), significantly higher motivation and significantly lower extraneous CL as well as higher germane CL during the learning process compared to a learning environment without WE. No differences between the conditions are expected regarding intrinsic CL. Furthermore, it is assumed (Hypothesis 2) that the integration of faded WE leads to significantly higher competence acquisition, significantly higher motivation, and lower extraneous CL as well as higher germane CL during the learning processes compared to non-faded WE. No differences between the conditions are expected with regard to intrinsic CL.

The study took place during the field trips of a university course on the application of a fluvial audit (FA) using the German working aid for mapping the morphology of rivers and their floodplains ( Bayerisches Landesamt für Umwelt, 2019 ). FA is the leading fluvial geomorphological tool for application to data collection contiguously along all watercourses of interest ( Walker et al., 2007 ). It is widely used because it is a key example of environmental conservation and monitoring that needs to be taught to students of selected study programs; thus, knowing about the most effective ways of learning is of high practical relevance.

3.1 Sample and design

3.1.1 sample.

The study was conducted with 62 science students and doctoral students of a German University (age M  = 24.03 years; SD  = 4.20; 36 females; 26 males). A total of 37 participants had already conducted a scientific observation and would rate their knowledge in this regard at a medium level ( M  = 3.32 out of 5; SD  = 0.88). Seven participants had already conducted an FA and would rate their knowledge in this regard at a medium level ( M  = 3.14 out of 5; SD  = 0.90). A total of 25 participants had no experience at all. Two participants had to be excluded from the sample afterward because no posttest results were available.

3.1.2 Design

The study has a 1-factorial quasi-experimental comparative research design and is conducted as a field experiment using a pre/posttest design. Participants were randomly assigned to one of three conditions: no WE ( n  = 20), faded WE ( n  = 20), and non-faded WE ( n  = 20).

3.2 Implementation and material

3.2.1 implementation.

The study started with an online kick-off meeting where two lecturers informed all students within an hour about the basics regarding the assessment of the structural integrity of the study river and the course of the field trip days to conduct an FA. Afterward, within 2 weeks, students self-studied via Moodle the FA following the German standard method according to the scoresheets of Bayerisches Landesamt für Umwelt (2019) . This independent preparation using the online presented documents was a necessary prerequisite for participation in the field days and was checked in the pre-testing. The preparatory online documents included six short videos and four PDF files on the content, guidance on the German protocol of the FA, general information on river landscapes, information about anthropogenic changes in stream morphology and the scoresheets for applying the FA. In these sheets, the river and its floodplain are subdivided into sections of 100 m in length. Each of these sections is evaluated by assessing 21 habitat factors related to flow characteristics and structural variability. The findings are then transferred into a scoring system for the description of structural integrity from 1 (natural) to 7 (highly modified). Habitat factors have a decisive influence on the living conditions of animals and plants in and around rivers. They included, e.g., variability in water depth, stream width, substratum diversity, or diversity of flow velocities.

3.2.2 Materials

On the field trip days, participants were handed a tablet and a paper-based FA worksheet (last accessed 21st September 2022). 1 This four-page assessment sheet was accompanied by a digital learning environment presented on Moodle that instructed the participants on mapping the water body structure and guided the scientific observation method. All three Moodle courses were identical in structure and design; the only difference was the implementation of the WE. Below, the course without WE are described first. The other two courses have an identical structure, but contain additional WE in the form of learning videos.

3.2.3 No worked example

After a short welcome and introduction to the course navigation, the FA started with the description of a short hypothetical scenario: Participants should take the role of an employee of an urban planning office that assesses the ecomorphological status of a small river near a Bavarian city. The river was divided into five sections that had to be mapped separately. The course was structured accordingly. At the beginning of each section, participants had to formulate and write down a research question, and according to hypotheses regarding the ecomorphological status of the river’s section, they had to collect data in this regard via the mapping sheet and then evaluate their data and draw a conclusion. Since this course serves as a control group, no WE videos supporting the scientific observation method were integrated. The layout of the course is structured like a book, where it is not possible to scroll back. This is important insofar as the participants do not have the possibility to revisit information in order to keep the conditions comparable as well as distinguishable.

3.2.4 Non-faded worked example

In the course with no-faded WE, three instructional videos are shown for each of the five sections. In each of the three videos, two steps of the scientific observation method are presented so that, finally, all six steps of scientific observation are demonstrated. The mapping of the first section starts after the general introduction (as described above) with the instruction to work on the first two steps of scientific observation: the formulation of a research question and hypotheses. To support this, a video of about 4 min explains the features of scientific sound research questions and hypotheses. To this aim, a practical example, including explanations and tips, is given regarding the formulation of research questions and hypotheses for this section (e.g., “To what extent does the building development and the closeness of the path to the water body have an influence on the structure of the water body?” Alternative hypothesis: It is assumed that the housing development and the closeness of the path to the water body have a negative influence on the water body structure. Null hypothesis: It is assumed that the housing development and the closeness of the path to the watercourse have no negative influence on the watercourse structure.). Participants should now formulate their own research questions and hypotheses, write them down in a text field at the end of the page, and then skip to the next page. The next two steps of scientific observation, planning and conducting, are explained in a short 4-min video. To this aim, a practical example including explanations and tips is given regarding planning and conducting scientific for this section (e.g., “It’s best to go through each evaluation category carefully one by one that way you are sure not to forget anything!”). Now, participants were asked to collect data for the first section using their paper-based FA worksheet. Participants individually surveyed the river and reported their results in the mapping sheet by ticking the respective boxes in it. After collecting this data, they returned to the digital learning environment to learn how to use these data by studying the last two steps of scientific observation, evaluation, and conclusion. The third 4-min video explained how to evaluate and interpret collected data. For this purpose, a practical example with explanations and tips is given regarding evaluating and interpreting data for this section (e.g., “What were the individual points that led to the assessment? Have there been points that were weighted more than others? Remember the introduction video!”). At the end of the page, participants could answer their before-stated research questions and hypotheses by evaluating their collected data and drawing a conclusion. This brings participants to the end of the first mapping section. Afterward, the cycle begins again with the second section of the river that has to be mapped. Again, participants had to conduct the steps of scientific observation, guided by WE videos, explaining the steps in slightly different wording or with different examples. A total of five sections are mapped, in which the structure of the learning environment and the videos follow the same procedure.

3.2.5 Faded worked example

The digital learning environment with the faded WE follow the same structure as the version with the non-faded WE. However, in this version, the information in the WE videos is successively reduced. In the first section, all three videos are identical to the version with the non-faded WE. In the second section, faded content was presented as follows: the tip at the end was omitted in all three videos. In the third section, the tip and the practical example were omitted. In the fourth and fifth sections, no more videos were presented, only the work instructions.

3.3 Procedure

The data collection took place on four continuous days on the university campus, with a maximum group size of 15 participants on each day. The students were randomly assigned to one of the three conditions (no WE vs. faded WE vs. non-faded WE). After a short introduction to the procedure, the participants were handed the paper-based FA worksheet and one tablet per person. Students scanned the QR code on the first page of the worksheet that opened the pretest questionnaire, which took about 20 min to complete. After completing the questionnaire, the group walked for about 15 min to the nearby small river that was to be mapped. Upon arrival, there was first a short introduction to the digital learning environment and a check that the login (via university account on Moodle) worked. During the next 4 h, the participants individually mapped five segments of the river using the cartography worksheet. They were guided through the steps of scientific observation using the digital learning environment on the tablet. The results of their scientific observation were logged within the digital learning environment. At the end of the digital learning environment, participants were directed to the posttest via a link. After completing the test, the tablets and mapping sheets were returned. Overall, the study took about 5 h per group each day.

3.4 Instruments

In the pretest, sociodemographic data (age and gender), the study domain and the number of study semesters were collected. Additionally, the previous scientific observation experience and the estimation of one’s own ability in this regard were assessed. For example, it was asked whether scientific observation had already been conducted and, if so, how the abilities were rated on a 5-point scale from very low to very high. Preparation for the FA on the basis of the learning material was assessed: Participants were asked whether they had studied all six videos and all four PDF documents, with the response options not at all, partially, and completely. Furthermore, a factual knowledge test about scientific observation and questions about self-determination theory was administered. The posttest used the same knowledge test, and additional questions on basic needs, situational interest, measures of CL and questions about the usefulness of the WE. All scales were presented online, and participants reached the questionnaire via QR code.

3.4.1 Scientific observation competence acquisition

For the factual knowledge (quantitative assessment of the scientific observation competence), a single-choice knowledge test with 12 questions was developed and used as pre- and posttest with a maximum score of 12 points. It assesses the learners’ knowledge of the scientific observation method regarding the steps of scientific observation, e.g., formulating research questions and hypotheses or developing a research design. The questions are based on Wahser (2008 , adapted by Koenen, 2014 ) and adapted to scientific observation: “Although you are sure that you have conducted the scientific observation correctly, an unexpected result turns up. What conclusion can you draw?” Each question has four answer options (one of which is correct) and, in addition, one “I do not know” option.

For the applied knowledge (quantified qualitative assessment of the scientific observation competence), students’ scientific observations written in the digital learning environment were analyzed. A coding scheme was used with the following codes: 0 = insufficient (text field is empty or includes only insufficient key points), 1 = sufficient (a research question and no hypotheses or research question and inappropriate hypotheses are stated), 2 = comprehensive (research question and appropriate hypothesis or research question and hypotheses are stated, but, e.g., incorrect null hypothesis), 3 = very comprehensive (correct research question, hypothesis and null hypothesis are stated). One example of a very comprehensive answer regarding the research question and hypothesis is: To what extent does the lack of riparian vegetation have an impact on water body structure? Hypothesis: The lack of shore vegetation has a negative influence on the water body structure. Null hypothesis: The lack of shore vegetation has no influence on the water body structure. Afterward, a sum score was calculated for each participant. Five times, a research question and hypotheses (steps 1 and 2 in the observation process) had to be formulated (5 × max. 3 points = 15 points), and five times, the research questions and hypotheses had to be answered (steps 5 and 6 in the observation process: evaluation and conclusion) (5 × max. 3 points = 15 points). Overall, participants could reach up to 30 points. Since the observation and evaluation criteria in data collection and analysis were strongly predetermined by the scoresheet, steps 3 and 4 of the observation process (planning and conducting) were not included in the analysis.

All 600 cases (60 participants, each 10 responses to code) were coded by the first author. For verification, 240 cases (24 randomly selected participants, eight from each course) were cross-coded by an external coder. In 206 of the coded cases, the raters agreed. The cases in which the raters did not agree were discussed together, and a solution was found. This results in Cohen’s κ = 0.858, indicating a high to very high level of agreement. This indicates that the category system is clearly formulated and that the individual units of analysis could be correctly assigned.

3.4.2 Self-determination index

For the calculation of the self-determination index (SDI-index), Thomas and Müller (2011) scale for self-determination was used in the pretest. The scale consists of four subscales: intrinsic motivation (five items; e.g., I engage with the workshop content because I enjoy it; reliability of alpha = 0.87), identified motivation (four items; e.g., I engage with the workshop content because it gives me more options when choosing a career; alpha = 0.84), introjected motivation (five items; e.g., I engage with the workshop content because otherwise I would have a guilty feeling; alpha = 0.79), and external motivation (three items, e.g., I engage with the workshop content because I simply have to learn it; alpha = 0.74). Participants could indicate their answers on a 5-point Likert scale ranging from 1 = completely disagree to 5 = completely agree. To calculate the SDI-index, the sum of the self-determined regulation styles (intrinsic and identified) is subtracted from the sum of the external regulation styles (introjected and external), where intrinsic and external regulation are scored two times ( Thomas and Müller, 2011 ).

3.4.3 Motivation

Basic needs were measured in the posttest with the scale by Willems and Lewalter (2011) . The scale consists of three subscales: perceived competence (four items; e.g., during the workshop, I felt that I could meet the requirements; alpha = 0.90), perceived autonomy (five items; e.g., during the workshop, I felt that I had a lot of freedom; alpha = 0.75), and perceived autonomy regarding personal wishes and goals (APWG) (four items; e.g., during the workshop, I felt that the workshop was how I wish it would be; alpha = 0.93). We added all three subscales to one overall basic needs scale (alpha = 0.90). Participants could indicate their answers on a 5-point Likert scale ranging from 1 = completely disagree to 5 = completely agree.

Situational interest was measured in the posttest with the 12-item scale by Lewalter and Knogler (2014 ; Knogler et al., 2015 ; Lewalter, 2020 ; alpha = 0.84). The scale consists of two subscales: catch (six items; e.g., I found the workshop exciting; alpha = 0.81) and hold (six items; e.g., I would like to learn more about parts of the workshop; alpha = 0.80). Participants could indicate their answers on a 5-point Likert scale ranging from 1 = completely disagree to 5 = completely agree.

3.4.4 Cognitive load

In the posttest, CL was used to examine the mental load during the learning process. The intrinsic CL (three items; e.g., this task was very complex; alpha = 0.70) and extraneous CL (three items; e.g., in this task, it is difficult to identify the most important information; alpha = 0.61) are measured with the scales from Klepsch et al. (2017) . The germane CL (two items; e.g., the learning session contained elements that supported me to better understand the learning material; alpha = 0.72) is measured with the scale from Leppink et al. (2013) . Participants could indicate their answers on a 5-point Likert scale ranging from 1 = completely disagree to 5 = completely agree.

3.4.5 Attitudes toward worked examples

To measure how effective participants rated the WE, we used two scales related to the WE videos as instructional support. The first scale from Renkl (2001) relates to the usefulness of WE. The scale consists of four items (e.g., the explanations were helpful; alpha = 0.71). Two items were recoded because they were formulated negatively. The second scale is from Wachsmuth (2020) and relates to the participant’s evaluation of the WE. The scale consists of nine items (e.g., I always did what was explained in the learning videos; alpha = 0.76). Four items were recoded because they were formulated negatively. Participants could indicate their answers on a 5-point Likert scale ranging from 1 = completely disagree to 5 = completely agree.

3.5 Data analysis

An ANOVA was used to calculate if the variable’s prior knowledge and SDI index differed between the three groups. However, as no significant differences between the conditions were found [prior factual knowledge: F (2, 59) = 0.15, p  = 0.865, η 2  = 0.00 self-determination index: F (2, 59) = 0.19, p  = 0.829, η 2  = 0.00], they were not included as covariates in subsequent analyses.

Furthermore, a repeated measure, one-way analysis of variance (ANOVA), was conducted to compare the three treatment groups (no WE vs. faded WE vs. non-faded WE) regarding the increase in factual knowledge about the scientific observation method from pretest to posttest.

A MANOVA (multivariate analysis) was calculated with the three groups (no WE vs. non-faded WE vs. faded WE) as a fixed factor and the dependent variables being the practical application of the scientific observation method (first research question), situational interest, basic needs (second research question), and CL (third research question).

Additionally, to determine differences in applied knowledge even among the three groups, Bonferroni-adjusted post-hoc analyses were conducted.

The descriptive statistics between the three groups in terms of prior factual knowledge about the scientific observation method and the self-determination index are shown in Table 1 . The descriptive statistics revealed only small, non-significant differences between the three groups in terms of factual knowledge.

Table 1 . Means (standard deviations) of factual knowledge tests (pre- and posttest) and self-determination index for the three different groups.

The results of the ANOVA revealed that the overall increase in factual knowledge from pre- to posttest just misses significance [ F (1, 57) = 3.68, p  = 0.060, η 2  = 0 0.06]. Furthermore, no significant differences between the groups were found regarding the acquisition of factual knowledge from pre- to posttest [ F (2, 57) = 2.93, p  = 0.062, η 2  = 0.09].

An analysis of the descriptive statistics showed that the largest differences between the groups were found in applied knowledge (qualitative evaluation) and extraneous load (see Table 2 ).

Table 2 . Means (standard deviations) of dependent variables with the three different groups.

Results of the MANOVA revealed significant overall differences between the three groups [ F (12, 106) = 2.59, p  = 0.005, η 2  = 0.23]. Significant effects were found for the application of knowledge [ F (2, 57) = 13.26, p  = <0.001, η 2  = 0.32]. Extraneous CL just missed significance [ F (2, 57) = 2.68, p  = 0.065, η 2  = 0.09]. There were no significant effects for situational interest [ F (2, 57) = 0.44, p  = 0.644, η 2  = 0.02], basic needs [ F (2, 57) = 1.22, p  = 0.302, η 2  = 0.04], germane CL [ F (2, 57) = 2.68, p  = 0.077, η 2  = 0.09], and intrinsic CL [ F (2, 57) = 0.28, p  = 0.757, η 2  = 0.01].

Bonferroni-adjusted post hoc analysis revealed that the group without WE had significantly lower scores in the evaluation of the applied knowledge than the group with non-faded WE ( p  = <0.001, M diff  = −8.90, 95% CI [−13.47, −4.33]) and then the group with faded WE ( p  = <0.001, M diff  = −7.40, 95% CI [−11.97, −2.83]). No difference was found between the groups with faded and non-faded WE ( p  = 1.00, M diff  = −1.50, 95% CI [−6.07, 3.07]).

The descriptive statistics regarding the perceived usefulness of WE and participants’ evaluation of the WE revealed that the group with the faded WE rated usefulness slightly higher than the participants with non-faded WE and also reported a more positive evaluation. However, the results of a MANOVA revealed no significant overall differences [ F (2, 37) = 0.32, p  = 0.732, η 2  = 0 0.02] (see Table 3 ).

Table 3 . Means (standard deviations) of dependent variables with the three different groups.

5 Discussion

This study investigated the use of WE to support students’ acquisition of science observation. Below, the research questions are answered, and the implications and limitations of the study are discussed.

5.1 Results on factual and applied knowledge

In terms of knowledge gain (RQ1), our findings revealed no significant differences in participants’ results of the factual knowledge test both across all three groups and specifically between the two experimental groups. These results are in contradiction with related literature where WE had a positive impact on knowledge acquisition ( Renkl, 2014 ) and faded WE are considered to be more effective in knowledge acquisition and transfer, in contrast to non-faded WE ( Renkl et al., 2000 ; Renkl, 2014 ). A limitation of the study is the fact that the participants already scored very high on the pretest, so participation in the intervention would likely not yield significant knowledge gains due to ceiling effects ( Staus et al., 2021 ). Yet, nearly half of the students reported being novices in the field prior to the study, suggesting that the difficulty of some test items might have been too low. Here, it would be important to revise the factual knowledge test, e.g., the difficulty of the distractors in further study.

Nevertheless, with regard to application knowledge, the results revealed large significant differences: Participants of the two experimental groups performed better in conducting scientific observation steps than participants of the control group. In the experimental groups, the non-faded WE group performed better than the faded WE group. However, the absence of significant differences between the two experimental groups suggests that faded and non-faded WE used as double-content WE are suitable to teach applied knowledge about scientific observation in the learning domain ( Koenen, 2014 ). Furthermore, our results differ from the findings of Renkl et al. (2000) , in which the faded version led to the highest knowledge transfer. Despite the fact that the non-faded WE performed best in our study, the faded version of the WE was also appropriate to improve learning, confirming the findings of Renkl (2014) and Hesser and Gregory (2015) .

5.2 Results on learners’ motivation

Regarding participants’ motivation (RQ2; situational interest and basic needs), no significant differences were found across all three groups or between the two experimental groups. However, descriptive results reveal slightly higher motivation in the two experimental groups than in the control group. In this regard, our results confirm existing literature on a descriptive level showing that WE lead to higher learning-relevant motivation ( Paas et al., 2005 ; Van Harsel et al., 2019 ). Additionally, both experimental groups rated the usefulness of the WE as high and reported a positive evaluation of the WE. Therefore, we assume that even non-faded WE do not lead to over-instruction. Regarding the descriptive tendency, a larger sample might yield significant results and detect even small effects in future investigations. However, because this study also focused on comprehensive qualitative data analysis, it was not possible to evaluate a larger sample in this study.

5.3 Results on cognitive load

Finally, CL did not vary significantly across all three groups (RQ3). However, differences in extraneous CL just slightly missed significance. In descriptive values, the control group reported the highest extrinsic and lowest germane CL. The faded WE group showed the lowest extrinsic CL and a similar germane CL as the non-faded WE group. These results are consistent with Paas et al. (2003) and Renkl (2014) , reporting that WE can help to reduce the extraneous CL and, in return, lead to an increase in germane CL. Again, these differences were just above the significance level, and it would be advantageous to retest with a larger sample to detect even small effects.

Taken together, our results only partially confirm H1: the integration of WE (both faded and non-faded WE) led to a higher acquisition of application knowledge than the control group without WE, but higher factual knowledge was not found. Furthermore, higher motivation or different CL was found on a descriptive level only. The control group provided the basis for comparison with the treatment in order to investigate if there is an effect at all and, if so, how large the effect is. This is an important point to assess whether the effort of implementing WE is justified. Additionally, regarding H2, our results reveal no significant differences between the two WE conditions. We assume that the high complexity of the FA could play a role in this regard, which might be hard to handle, especially for beginners, so learners could benefit from support throughout (i.e., non-faded WE).

In addition to the limitations already mentioned, it must be noted that only one exemplary topic was investigated, and the sample only consisted of students. Since only the learning domain of the double-content WE was investigated, the exemplifying domain could also be analyzed, or further variables like motivation could be included in further studies. Furthermore, the influence of learners’ prior knowledge on learning with WE could be investigated, as studies have found that WE are particularly beneficial in the initial acquisition of cognitive skills ( Kalyuga et al., 2001 ).

6 Conclusion

Overall, the results of the current study suggest a beneficial role for WE in supporting the application of scientific observation steps. A major implication of these findings is that both faded and non-faded WE should be considered, as no general advantage of faded WE over non-faded WE was found. This information can be used to develop targeted interventions aimed at the support of scientific observation skills.

Data availability statement

The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.

Ethics statement

Ethical approval was not required for the study involving human participants in accordance with the local legislation and institutional requirements. Written informed consent to participate in this study was not required from the participants in accordance with the national legislation and the institutional requirements.

Author contributions

ML: Writing – original draft. SM: Writing – review & editing. JP: Writing – review & editing. JG: Writing – review & editing. DL: Writing – review & editing.

The author(s) declare that no financial support was received for the research, authorship, and/or publication of this article.

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/feduc.2024.1293516/full#supplementary-material

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Keywords: digital media, worked examples, scientific observation, motivation, cognitive load

Citation: Lechner M, Moser S, Pander J, Geist J and Lewalter D (2024) Learning scientific observation with worked examples in a digital learning environment. Front. Educ . 9:1293516. doi: 10.3389/feduc.2024.1293516

Received: 13 September 2023; Accepted: 29 February 2024; Published: 18 March 2024.

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*Correspondence: Miriam Lechner, [email protected]