what is the meaning of research hypothesis

What Is A Research (Scientific) Hypothesis? A plain-language explainer + examples

By:  Derek Jansen (MBA)  | Reviewed By: Dr Eunice Rautenbach | June 2020

If you’re new to the world of research, or it’s your first time writing a dissertation or thesis, you’re probably noticing that the words “research hypothesis” and “scientific hypothesis” are used quite a bit, and you’re wondering what they mean in a research context .

“Hypothesis” is one of those words that people use loosely, thinking they understand what it means. However, it has a very specific meaning within academic research. So, it’s important to understand the exact meaning before you start hypothesizing. 

Research Hypothesis 101

• What is a hypothesis ?
• What is a research hypothesis (scientific hypothesis)?
• Requirements for a research hypothesis
• Definition of a research hypothesis
• The null hypothesis

What is a hypothesis?

Let’s start with the general definition of a hypothesis (not a research hypothesis or scientific hypothesis), according to the Cambridge Dictionary:

Hypothesis: an idea or explanation for something that is based on known facts but has not yet been proved.

In other words, it’s a statement that provides an explanation for why or how something works, based on facts (or some reasonable assumptions), but that has not yet been specifically tested . For example, a hypothesis might look something like this:

Hypothesis: sleep impacts academic performance.

This statement predicts that academic performance will be influenced by the amount and/or quality of sleep a student engages in – sounds reasonable, right? It’s based on reasonable assumptions , underpinned by what we currently know about sleep and health (from the existing literature). So, loosely speaking, we could call it a hypothesis, at least by the dictionary definition.

But that’s not good enough…

Unfortunately, that’s not quite sophisticated enough to describe a research hypothesis (also sometimes called a scientific hypothesis), and it wouldn’t be acceptable in a dissertation, thesis or research paper . In the world of academic research, a statement needs a few more criteria to constitute a true research hypothesis .

What is a research hypothesis?

A research hypothesis (also called a scientific hypothesis) is a statement about the expected outcome of a study (for example, a dissertation or thesis). To constitute a quality hypothesis, the statement needs to have three attributes – specificity , clarity and testability .

Let’s take a look at these more closely.

Need a helping hand?

Hypothesis Essential #1: Specificity & Clarity

A good research hypothesis needs to be extremely clear and articulate about both what’ s being assessed (who or what variables are involved ) and the expected outcome (for example, a difference between groups, a relationship between variables, etc.).

Let’s stick with our sleepy students example and look at how this statement could be more specific and clear.

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.

As you can see, the statement is very specific as it identifies the variables involved (sleep hours and test grades), the parties involved (two groups of students), as well as the predicted relationship type (a positive relationship). There’s no ambiguity or uncertainty about who or what is involved in the statement, and the expected outcome is clear.

Contrast that to the original hypothesis we looked at – “Sleep impacts academic performance” – and you can see the difference. “Sleep” and “academic performance” are both comparatively vague , and there’s no indication of what the expected relationship direction is (more sleep or less sleep). As you can see, specificity and clarity are key.

Hypothesis Essential #2: Testability (Provability)

A statement must be testable to qualify as a research hypothesis. In other words, there needs to be a way to prove (or disprove) the statement. If it’s not testable, it’s not a hypothesis – simple as that.

For example, consider the hypothesis we mentioned earlier:

Hypothesis: Students who sleep at least 8 hours per night will, on average, achieve higher grades in standardised tests than students who sleep less than 8 hours a night.  

We could test this statement by undertaking a quantitative study involving two groups of students, one that gets 8 or more hours of sleep per night for a fixed period, and one that gets less. We could then compare the standardised test results for both groups to see if there’s a statistically significant difference. 

Again, if you compare this to the original hypothesis we looked at – “Sleep impacts academic performance” – you can see that it would be quite difficult to test that statement, primarily because it isn’t specific enough. How much sleep? By who? What type of academic performance?

So, remember the mantra – if you can’t test it, it’s not a hypothesis 🙂

Defining A Research Hypothesis

You’re still with us? Great! Let’s recap and pin down a clear definition of a hypothesis.

A research hypothesis (or scientific hypothesis) is a statement about an expected relationship between variables, or explanation of an occurrence, that is clear, specific and testable.

So, when you write up hypotheses for your dissertation or thesis, make sure that they meet all these criteria. If you do, you’ll not only have rock-solid hypotheses but you’ll also ensure a clear focus for your entire research project.

What about the null hypothesis?

You may have also heard the terms null hypothesis , alternative hypothesis, or H-zero thrown around. At a simple level, the null hypothesis is the counter-proposal to the original hypothesis.

For example, if the hypothesis predicts that there is a relationship between two variables (for example, sleep and academic performance), the null hypothesis would predict that there is no relationship between those variables.

At a more technical level, the null hypothesis proposes that no statistical significance exists in a set of given observations and that any differences are due to chance alone.

And there you have it – hypotheses in a nutshell. 

If you have any questions, be sure to leave a comment below and we’ll do our best to help you. If you need hands-on help developing and testing your hypotheses, consider our private coaching service , where we hold your hand through the research journey.

Psst... there’s more!

This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

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16 Comments

Very useful information. I benefit more from getting more information in this regard.

Very great insight,educative and informative. Please give meet deep critics on many research data of public international Law like human rights, environment, natural resources, law of the sea etc

In a book I read a distinction is made between null, research, and alternative hypothesis. As far as I understand, alternative and research hypotheses are the same. Can you please elaborate? Best Afshin

This is a self explanatory, easy going site. I will recommend this to my friends and colleagues.

Very good definition. How can I cite your definition in my thesis? Thank you. Is nul hypothesis compulsory in a research?

It’s a counter-proposal to be proven as a rejection

Please what is the difference between alternate hypothesis and research hypothesis?

It is a very good explanation. However, it limits hypotheses to statistically tasteable ideas. What about for qualitative researches or other researches that involve quantitative data that don’t need statistical tests?

In qualitative research, one typically uses propositions, not hypotheses.

could you please elaborate it more

I’ve benefited greatly from these notes, thank you.

This is very helpful

well articulated ideas are presented here, thank you for being reliable sources of information

Excellent. Thanks for being clear and sound about the research methodology and hypothesis (quantitative research)

I have only a simple question regarding the null hypothesis. – Is the null hypothesis (Ho) known as the reversible hypothesis of the alternative hypothesis (H1? – How to test it in academic research?

this is very important note help me much more

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

About the author

Muhammad Hassan

Researcher, Academic Writer, Web developer

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Research Hypothesis: What It Is, Types + How to Develop?

A research study starts with a question. Researchers worldwide ask questions and create research hypotheses. The effectiveness of research relies on developing a good research hypothesis. Examples of research hypotheses can guide researchers in writing effective ones.

In this blog, we’ll learn what a research hypothesis is, why it’s important in research, and the different types used in science. We’ll also guide you through creating your research hypothesis and discussing ways to test and evaluate it.

What is a Research Hypothesis?

A hypothesis is like a guess or idea that you suggest to check if it’s true. A research hypothesis is a statement that brings up a question and predicts what might happen.

It’s really important in the scientific method and is used in experiments to figure things out. Essentially, it’s an educated guess about how things are connected in the research.

A research hypothesis usually includes pointing out the independent variable (the thing they’re changing or studying) and the dependent variable (the result they’re measuring or watching). It helps plan how to gather and analyze data to see if there’s evidence to support or deny the expected connection between these variables.

Importance of Hypothesis in Research

Hypotheses are really important in research. They help design studies, allow for practical testing, and add to our scientific knowledge. Their main role is to organize research projects, making them purposeful, focused, and valuable to the scientific community. Let’s look at some key reasons why they matter:

• A research hypothesis helps test theories.

A hypothesis plays a pivotal role in the scientific method by providing a basis for testing existing theories. For example, a hypothesis might test the predictive power of a psychological theory on human behavior.

• It serves as a great platform for investigation activities.

It serves as a launching pad for investigation activities, which offers researchers a clear starting point. A research hypothesis can explore the relationship between exercise and stress reduction.

• Hypothesis guides the research work or study.

A well-formulated hypothesis guides the entire research process. It ensures that the study remains focused and purposeful. For instance, a hypothesis about the impact of social media on interpersonal relationships provides clear guidance for a study.

• Hypothesis sometimes suggests theories.

In some cases, a hypothesis can suggest new theories or modifications to existing ones. For example, a hypothesis testing the effectiveness of a new drug might prompt a reconsideration of current medical theories.

• It helps in knowing the data needs.

A hypothesis clarifies the data requirements for a study, ensuring that researchers collect the necessary information—a hypothesis guiding the collection of demographic data to analyze the influence of age on a particular phenomenon.

• The hypothesis explains social phenomena.

Hypotheses are instrumental in explaining complex social phenomena. For instance, a hypothesis might explore the relationship between economic factors and crime rates in a given community.

• Hypothesis provides a relationship between phenomena for empirical Testing.

Hypotheses establish clear relationships between phenomena, paving the way for empirical testing. An example could be a hypothesis exploring the correlation between sleep patterns and academic performance.

• It helps in knowing the most suitable analysis technique.

A hypothesis guides researchers in selecting the most appropriate analysis techniques for their data. For example, a hypothesis focusing on the effectiveness of a teaching method may lead to the choice of statistical analyses best suited for educational research.

Characteristics of a Good Research Hypothesis

A hypothesis is a specific idea that you can test in a study. It often comes from looking at past research and theories. A good hypothesis usually starts with a research question that you can explore through background research. For it to be effective, consider these key characteristics:

• Clear and Focused Language: A good hypothesis uses clear and focused language to avoid confusion and ensure everyone understands it.
• Related to the Research Topic: The hypothesis should directly relate to the research topic, acting as a bridge between the specific question and the broader study.
• Testable: An effective hypothesis can be tested, meaning its prediction can be checked with real data to support or challenge the proposed relationship.
• Potential for Exploration: A good hypothesis often comes from a research question that invites further exploration. Doing background research helps find gaps and potential areas to investigate.
• Includes Variables: The hypothesis should clearly state both the independent and dependent variables, specifying the factors being studied and the expected outcomes.
• Ethical Considerations: Check if variables can be manipulated without breaking ethical standards. It’s crucial to maintain ethical research practices.
• Predicts Outcomes: The hypothesis should predict the expected relationship and outcome, acting as a roadmap for the study and guiding data collection and analysis.
• Simple and Concise: A good hypothesis avoids unnecessary complexity and is simple and concise, expressing the essence of the proposed relationship clearly.
• Clear and Assumption-Free: The hypothesis should be clear and free from assumptions about the reader’s prior knowledge, ensuring universal understanding.
• Observable and Testable Results: A strong hypothesis implies research that produces observable and testable results, making sure the study’s outcomes can be effectively measured and analyzed.

When you use these characteristics as a checklist, it can help you create a good research hypothesis. It’ll guide improving and strengthening the hypothesis, identifying any weaknesses, and making necessary changes. Crafting a hypothesis with these features helps you conduct a thorough and insightful research study.

Types of Research Hypotheses

The research hypothesis comes in various types, each serving a specific purpose in guiding the scientific investigation. Knowing the differences will make it easier for you to create your own hypothesis. Here’s an overview of the common types:

01. Null Hypothesis

The null hypothesis states that there is no connection between two considered variables or that two groups are unrelated. As discussed earlier, a hypothesis is an unproven assumption lacking sufficient supporting data. It serves as the statement researchers aim to disprove. It is testable, verifiable, and can be rejected.

For example, if you’re studying the relationship between Project A and Project B, assuming both projects are of equal standard is your null hypothesis. It needs to be specific for your study.

02. Alternative Hypothesis

The alternative hypothesis is basically another option to the null hypothesis. It involves looking for a significant change or alternative that could lead you to reject the null hypothesis. It’s a different idea compared to the null hypothesis.

When you create a null hypothesis, you’re making an educated guess about whether something is true or if there’s a connection between that thing and another variable. If the null view suggests something is correct, the alternative hypothesis says it’s incorrect. 

For instance, if your null hypothesis is “I’m going to be $1000 richer,” the alternative hypothesis would be “I’m not going to get $1000 or be richer.”

03. Directional Hypothesis

The directional hypothesis predicts the direction of the relationship between independent and dependent variables. They specify whether the effect will be positive or negative.

If you increase your study hours, you will experience a positive association with your exam scores. This hypothesis suggests that as you increase the independent variable (study hours), there will also be an increase in the dependent variable (exam scores).

04. Non-directional Hypothesis

The non-directional hypothesis predicts the existence of a relationship between variables but does not specify the direction of the effect. It suggests that there will be a significant difference or relationship, but it does not predict the nature of that difference.

For example, you will find no notable difference in test scores between students who receive the educational intervention and those who do not. However, once you compare the test scores of the two groups, you will notice an important difference.

05. Simple Hypothesis

A simple hypothesis predicts a relationship between one dependent variable and one independent variable without specifying the nature of that relationship. It’s simple and usually used when we don’t know much about how the two things are connected.

For example, if you adopt effective study habits, you will achieve higher exam scores than those with poor study habits.

06. Complex Hypothesis

A complex hypothesis is an idea that specifies a relationship between multiple independent and dependent variables. It is a more detailed idea than a simple hypothesis.

While a simple view suggests a straightforward cause-and-effect relationship between two things, a complex hypothesis involves many factors and how they’re connected to each other.

For example, when you increase your study time, you tend to achieve higher exam scores. The connection between your study time and exam performance is affected by various factors, including the quality of your sleep, your motivation levels, and the effectiveness of your study techniques.

If you sleep well, stay highly motivated, and use effective study strategies, you may observe a more robust positive correlation between the time you spend studying and your exam scores, unlike those who may lack these factors.

07. Associative Hypothesis

An associative hypothesis proposes a connection between two things without saying that one causes the other. Basically, it suggests that when one thing changes, the other changes too, but it doesn’t claim that one thing is causing the change in the other.

For example, you will likely notice higher exam scores when you increase your study time. You can recognize an association between your study time and exam scores in this scenario.

Your hypothesis acknowledges a relationship between the two variables—your study time and exam scores—without asserting that increased study time directly causes higher exam scores. You need to consider that other factors, like motivation or learning style, could affect the observed association.

08. Causal Hypothesis

A causal hypothesis proposes a cause-and-effect relationship between two variables. It suggests that changes in one variable directly cause changes in another variable.

For example, when you increase your study time, you experience higher exam scores. This hypothesis suggests a direct cause-and-effect relationship, indicating that the more time you spend studying, the higher your exam scores. It assumes that changes in your study time directly influence changes in your exam performance.

09. Empirical Hypothesis

An empirical hypothesis is a statement based on things we can see and measure. It comes from direct observation or experiments and can be tested with real-world evidence. If an experiment proves a theory, it supports the idea and shows it’s not just a guess. This makes the statement more reliable than a wild guess.

For example, if you increase the dosage of a certain medication, you might observe a quicker recovery time for patients. Imagine you’re in charge of a clinical trial. In this trial, patients are given varying dosages of the medication, and you measure and compare their recovery times. This allows you to directly see the effects of different dosages on how fast patients recover.

This way, you can create a research hypothesis: “Increasing the dosage of a certain medication will lead to a faster recovery time for patients.”

10. Statistical Hypothesis

A statistical hypothesis is a statement or assumption about a population parameter that is the subject of an investigation. It serves as the basis for statistical analysis and testing. It is often tested using statistical methods to draw inferences about the larger population.

In a hypothesis test, statistical evidence is collected to either reject the null hypothesis in favor of the alternative hypothesis or fail to reject the null hypothesis due to insufficient evidence.

For example, let’s say you’re testing a new medicine. Your hypothesis could be that the medicine doesn’t really help patients get better. So, you collect data and use statistics to see if your guess is right or if the medicine actually makes a difference.

If the data strongly shows that the medicine does help, you say your guess was wrong, and the medicine does make a difference. But if the proof isn’t strong enough, you can stick with your original guess because you didn’t get enough evidence to change your mind.

How to Develop a Research Hypotheses?

Step 1: identify your research problem or topic..

Define the area of interest or the problem you want to investigate. Make sure it’s clear and well-defined.

Start by asking a question about your chosen topic. Consider the limitations of your research and create a straightforward problem related to your topic. Once you’ve done that, you can develop and test a hypothesis with evidence.

Step 2: Conduct a literature review

Review existing literature related to your research problem. This will help you understand the current state of knowledge in the field, identify gaps, and build a foundation for your hypothesis. Consider the following questions:

• What existing research has been conducted on your chosen topic?
• Are there any gaps or unanswered questions in the current literature?
• How will the existing literature contribute to the foundation of your research?

Step 3: Formulate your research question

Based on your literature review, create a specific and concise research question that addresses your identified problem. Your research question should be clear, focused, and relevant to your field of study.

Step 4: Identify variables

Determine the key variables involved in your research question. Variables are the factors or phenomena that you will study and manipulate to test your hypothesis.

• Independent Variable: The variable you manipulate or control.
• Dependent Variable: The variable you measure to observe the effect of the independent variable.

Step 5: State the Null hypothesis

The null hypothesis is a statement that there is no significant difference or effect. It serves as a baseline for comparison with the alternative hypothesis.

Step 6: Select appropriate methods for testing the hypothesis

Choose research methods that align with your study objectives, such as experiments, surveys, or observational studies. The selected methods enable you to test your research hypothesis effectively.

Creating a research hypothesis usually takes more than one try. Expect to make changes as you collect data. It’s normal to test and say no to a few hypotheses before you find the right answer to your research question.

Testing and Evaluating Hypotheses

Testing hypotheses is a really important part of research. It’s like the practical side of things. Here, real-world evidence will help you determine how different things are connected. Let’s explore the main steps in hypothesis testing:

• State your research hypothesis.

Before testing, clearly articulate your research hypothesis. This involves framing both a null hypothesis, suggesting no significant effect or relationship, and an alternative hypothesis, proposing the expected outcome.

• Collect data strategically.

Plan how you will gather information in a way that fits your study. Make sure your data collection method matches the things you’re studying.

Whether through surveys, observations, or experiments, this step demands precision and adherence to the established methodology. The quality of data collected directly influences the credibility of study outcomes.

• Perform an appropriate statistical test.

Choose a statistical test that aligns with the nature of your data and the hypotheses being tested. Whether it’s a t-test, chi-square test, ANOVA, or regression analysis, selecting the right statistical tool is paramount for accurate and reliable results.

• Decide if your idea was right or wrong.

Following the statistical analysis, evaluate the results in the context of your null hypothesis. You need to decide if you should reject your null hypothesis or not.

• Share what you found.

When discussing what you found in your research, be clear and organized. Say whether your idea was supported or not, and talk about what your results mean. Also, mention any limits to your study and suggest ideas for future research.

The Role of QuestionPro to Develop a Good Research Hypothesis

QuestionPro is a survey and research platform that provides tools for creating, distributing, and analyzing surveys. It plays a crucial role in the research process, especially when you’re in the initial stages of hypothesis development. Here’s how QuestionPro can help you to develop a good research hypothesis:

• Survey design and data collection: You can use the platform to create targeted questions that help you gather relevant data.
• Exploratory research: Through surveys and feedback mechanisms on QuestionPro, you can conduct exploratory research to understand the landscape of a particular subject.
• Literature review and background research: QuestionPro surveys can collect sample population opinions, experiences, and preferences. This data and a thorough literature evaluation can help you generate a well-grounded hypothesis by improving your research knowledge.
• Identifying variables: Using targeted survey questions, you can identify relevant variables related to their research topic.
• Testing assumptions: You can use surveys to informally test certain assumptions or hypotheses before formalizing a research hypothesis.
• Data analysis tools: QuestionPro provides tools for analyzing survey data. You can use these tools to identify the collected data’s patterns, correlations, or trends.
• Refining your hypotheses: As you collect data through QuestionPro, you can adjust your hypotheses based on the real-world responses you receive.

A research hypothesis is like a guide for researchers in science. It’s a well-thought-out idea that has been thoroughly tested. This idea is crucial as researchers can explore different fields, such as medicine, social sciences, and natural sciences. The research hypothesis links theories to real-world evidence and gives researchers a clear path to explore and make discoveries.

QuestionPro Research Suite is a helpful tool for researchers. It makes creating surveys, collecting data, and analyzing information easily. It supports all kinds of research, from exploring new ideas to forming hypotheses. With a focus on using data, it helps researchers do their best work.

Are you interested in learning more about QuestionPro Research Suite? Take advantage of QuestionPro’s free trial to get an initial look at its capabilities and realize the full potential of your research efforts.

LEARN MORE         FREE TRIAL

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How to Write a Great Hypothesis

Hypothesis Definition, Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis.

• Operationalization

Hypothesis Types

Hypotheses examples.

• Collecting Data

A hypothesis is a tentative statement about the relationship between two or more variables. It is a specific, testable prediction about what you expect to happen in a study. It is a preliminary answer to your question that helps guide the research process.

Consider a study designed to examine the relationship between sleep deprivation and test performance. The hypothesis might be: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

At a Glance

A hypothesis is crucial to scientific research because it offers a clear direction for what the researchers are looking to find. This allows them to design experiments to test their predictions and add to our scientific knowledge about the world. This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. At this point, researchers then begin to develop a testable hypothesis.

Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore numerous factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk adage that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

How to Formulate a Good Hypothesis

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis. In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

The Importance of Operational Definitions

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

Operational definitions are specific definitions for all relevant factors in a study. This process helps make vague or ambiguous concepts detailed and measurable.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in various ways. Clearly defining these variables and how they are measured helps ensure that other researchers can replicate your results.

Replicability

One of the basic principles of any type of scientific research is that the results must be replicable.

Replication means repeating an experiment in the same way to produce the same results. By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. For example, how would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

To measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming others. The researcher might utilize a simulated task to measure aggressiveness in this situation.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type suggests a relationship between three or more variables, such as two independent and dependent variables.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative population sample and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."
• "Children who receive a new reading intervention will have higher reading scores than students who do not receive the intervention."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "There is no difference in anxiety levels between people who take St. John's wort supplements and those who do not."
• "There is no difference in scores on a memory recall task between children and adults."
• "There is no difference in aggression levels between children who play first-person shooter games and those who do not."

Examples of an alternative hypothesis:

• "People who take St. John's wort supplements will have less anxiety than those who do not."
• "Adults will perform better on a memory task than children."
• "Children who play first-person shooter games will show higher levels of aggression than children who do not." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when  conducting an experiment is difficult or impossible. These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a  correlational study  can examine how the variables are related. This research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Thompson WH, Skau S. On the scope of scientific hypotheses .  R Soc Open Sci . 2023;10(8):230607. doi:10.1098/rsos.230607

Taran S, Adhikari NKJ, Fan E. Falsifiability in medicine: what clinicians can learn from Karl Popper [published correction appears in Intensive Care Med. 2021 Jun 17;:].  Intensive Care Med . 2021;47(9):1054-1056. doi:10.1007/s00134-021-06432-z

Eyler AA. Research Methods for Public Health . 1st ed. Springer Publishing Company; 2020. doi:10.1891/9780826182067.0004

Nosek BA, Errington TM. What is replication ?  PLoS Biol . 2020;18(3):e3000691. doi:10.1371/journal.pbio.3000691

Aggarwal R, Ranganathan P. Study designs: Part 2 - Descriptive studies .  Perspect Clin Res . 2019;10(1):34-36. doi:10.4103/picr.PICR_154_18

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

How to Write a Research Hypothesis

• Research Process
• Peer Review

Since grade school, we've all been familiar with hypotheses. The hypothesis is an essential step of the scientific method. But what makes an effective research hypothesis, how do you create one, and what types of hypotheses are there? We answer these questions and more.

Updated on April 27, 2022

What is a research hypothesis?

General hypothesis.

Since grade school, we've all been familiar with the term “hypothesis.” A hypothesis is a fact-based guess or prediction that has not been proven. It is an essential step of the scientific method. The hypothesis of a study is a drive for experimentation to either prove the hypothesis or dispute it.

Research Hypothesis

A research hypothesis is more specific than a general hypothesis. It is an educated, expected prediction of the outcome of a study that is testable.

What makes an effective research hypothesis?

A good research hypothesis is a clear statement of the relationship between a dependent variable(s) and independent variable(s) relevant to the study that can be disproven.

Research hypothesis checklist

Once you've written a possible hypothesis, make sure it checks the following boxes:

• It must be testable: You need a means to prove your hypothesis. If you can't test it, it's not a hypothesis.
• It must include a dependent and independent variable: At least one independent variable ( cause ) and one dependent variable ( effect ) must be included.
• The language must be easy to understand: Be as clear and concise as possible. Nothing should be left to interpretation.
• It must be relevant to your research topic: You probably shouldn't be talking about cats and dogs if your research topic is outer space. Stay relevant to your topic.

How to create an effective research hypothesis

Pose it as a question first.

Start your research hypothesis from a journalistic approach. Ask one of the five W's: Who, what, when, where, or why.

A possible initial question could be: Why is the sky blue?

Do the preliminary research

Once you have a question in mind, read research around your topic. Collect research from academic journals.

If you're looking for information about the sky and why it is blue, research information about the atmosphere, weather, space, the sun, etc.

Write a draft hypothesis

Once you're comfortable with your subject and have preliminary knowledge, create a working hypothesis. Don't stress much over this. Your first hypothesis is not permanent. Look at it as a draft.

Your first draft of a hypothesis could be: Certain molecules in the Earth's atmosphere are responsive to the sky being the color blue.

Make your working draft perfect

Take your working hypothesis and make it perfect. Narrow it down to include only the information listed in the “Research hypothesis checklist” above.

Now that you've written your working hypothesis, narrow it down. Your new hypothesis could be: Light from the sun hitting oxygen molecules in the sky makes the color of the sky appear blue.

Write a null hypothesis

Your null hypothesis should be the opposite of your research hypothesis. It should be able to be disproven by your research.

In this example, your null hypothesis would be: Light from the sun hitting oxygen molecules in the sky does not make the color of the sky appear blue.

Why is it important to have a clear, testable hypothesis?

One of the main reasons a manuscript can be rejected from a journal is because of a weak hypothesis. “Poor hypothesis, study design, methodology, and improper use of statistics are other reasons for rejection of a manuscript,” says Dr. Ish Kumar Dhammi and Dr. Rehan-Ul-Haq in Indian Journal of Orthopaedics.

According to Dr. James M. Provenzale in American Journal of Roentgenology , “The clear declaration of a research question (or hypothesis) in the Introduction is critical for reviewers to understand the intent of the research study. It is best to clearly state the study goal in plain language (for example, “We set out to determine whether condition x produces condition y.”) An insufficient problem statement is one of the more common reasons for manuscript rejection.”

Characteristics that make a hypothesis weak include:

• Unclear variables
• Unoriginality
• Too general
• Too specific

A weak hypothesis leads to weak research and methods . The goal of a paper is to prove or disprove a hypothesis - or to prove or disprove a null hypothesis. If the hypothesis is not a dependent variable of what is being studied, the paper's methods should come into question.

A strong hypothesis is essential to the scientific method. A hypothesis states an assumed relationship between at least two variables and the experiment then proves or disproves that relationship with statistical significance. Without a proven and reproducible relationship, the paper feeds into the reproducibility crisis. Learn more about writing for reproducibility .

In a study published in The Journal of Obstetrics and Gynecology of India by Dr. Suvarna Satish Khadilkar, she reviewed 400 rejected manuscripts to see why they were rejected. Her studies revealed that poor methodology was a top reason for the submission having a final disposition of rejection.

Aside from publication chances, Dr. Gareth Dyke believes a clear hypothesis helps efficiency.

“Developing a clear and testable hypothesis for your research project means that you will not waste time, energy, and money with your work,” said Dyke. “Refining a hypothesis that is both meaningful, interesting, attainable, and testable is the goal of all effective research.”

Types of research hypotheses

There can be overlap in these types of hypotheses.

Simple hypothesis

A simple hypothesis is a hypothesis at its most basic form. It shows the relationship of one independent and one independent variable.

Example: Drinking soda (independent variable) every day leads to obesity (dependent variable).

Complex hypothesis

A complex hypothesis shows the relationship of two or more independent and dependent variables.

Example: Drinking soda (independent variable) every day leads to obesity (dependent variable) and heart disease (dependent variable).

Directional hypothesis

A directional hypothesis guesses which way the results of an experiment will go. It uses words like increase, decrease, higher, lower, positive, negative, more, or less. It is also frequently used in statistics.

Example: Humans exposed to radiation have a higher risk of cancer than humans not exposed to radiation.

Non-directional hypothesis

A non-directional hypothesis says there will be an effect on the dependent variable, but it does not say which direction.

Associative hypothesis

An associative hypothesis says that when one variable changes, so does the other variable.

Alternative hypothesis

An alternative hypothesis states that the variables have a relationship.

• The opposite of a null hypothesis

Example: An apple a day keeps the doctor away.

Null hypothesis

A null hypothesis states that there is no relationship between the two variables. It is posed as the opposite of what the alternative hypothesis states.

Researchers use a null hypothesis to work to be able to reject it. A null hypothesis:

• Can never be proven
• Can only be rejected
• Is the opposite of an alternative hypothesis

Example: An apple a day does not keep the doctor away.

Logical hypothesis

A logical hypothesis is a suggested explanation while using limited evidence.

Example: Bats can navigate in the dark better than tigers.

In this hypothesis, the researcher knows that tigers cannot see in the dark, and bats mostly live in darkness.

Empirical hypothesis

An empirical hypothesis is also called a “working hypothesis.” It uses the trial and error method and changes around the independent variables.

• An apple a day keeps the doctor away.
• Two apples a day keep the doctor away.
• Three apples a day keep the doctor away.

In this case, the research changes the hypothesis as the researcher learns more about his/her research.

Statistical hypothesis

A statistical hypothesis is a look of a part of a population or statistical model. This type of hypothesis is especially useful if you are making a statement about a large population. Instead of having to test the entire population of Illinois, you could just use a smaller sample of people who live there.

Example: 70% of people who live in Illinois are iron deficient.

Causal hypothesis

A causal hypothesis states that the independent variable will have an effect on the dependent variable.

Example: Using tobacco products causes cancer.

Final thoughts

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What is and How to Write a Good Hypothesis in Research?

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Table of Contents

One of the most important aspects of conducting research is constructing a strong hypothesis. But what makes a hypothesis in research effective? In this article, we’ll look at the difference between a hypothesis and a research question, as well as the elements of a good hypothesis in research. We’ll also include some examples of effective hypotheses, and what pitfalls to avoid.

What is a Hypothesis in Research?

Simply put, a hypothesis is a research question that also includes the predicted or expected result of the research. Without a hypothesis, there can be no basis for a scientific or research experiment. As such, it is critical that you carefully construct your hypothesis by being deliberate and thorough, even before you set pen to paper. Unless your hypothesis is clearly and carefully constructed, any flaw can have an adverse, and even grave, effect on the quality of your experiment and its subsequent results.

Research Question vs Hypothesis

It’s easy to confuse research questions with hypotheses, and vice versa. While they’re both critical to the Scientific Method, they have very specific differences. Primarily, a research question, just like a hypothesis, is focused and concise. But a hypothesis includes a prediction based on the proposed research, and is designed to forecast the relationship of and between two (or more) variables. Research questions are open-ended, and invite debate and discussion, while hypotheses are closed, e.g. “The relationship between A and B will be C.”

A hypothesis is generally used if your research topic is fairly well established, and you are relatively certain about the relationship between the variables that will be presented in your research. Since a hypothesis is ideally suited for experimental studies, it will, by its very existence, affect the design of your experiment. The research question is typically used for new topics that have not yet been researched extensively. Here, the relationship between different variables is less known. There is no prediction made, but there may be variables explored. The research question can be casual in nature, simply trying to understand if a relationship even exists, descriptive or comparative.

How to Write Hypothesis in Research

Writing an effective hypothesis starts before you even begin to type. Like any task, preparation is key, so you start first by conducting research yourself, and reading all you can about the topic that you plan to research. From there, you’ll gain the knowledge you need to understand where your focus within the topic will lie.

Remember that a hypothesis is a prediction of the relationship that exists between two or more variables. Your job is to write a hypothesis, and design the research, to “prove” whether or not your prediction is correct. A common pitfall is to use judgments that are subjective and inappropriate for the construction of a hypothesis. It’s important to keep the focus and language of your hypothesis objective.

An effective hypothesis in research is clearly and concisely written, and any terms or definitions clarified and defined. Specific language must also be used to avoid any generalities or assumptions.

Use the following points as a checklist to evaluate the effectiveness of your research hypothesis:

• Predicts the relationship and outcome
• Simple and concise – avoid wordiness
• Clear with no ambiguity or assumptions about the readers’ knowledge
• Observable and testable results
• Relevant and specific to the research question or problem

Research Hypothesis Example

Perhaps the best way to evaluate whether or not your hypothesis is effective is to compare it to those of your colleagues in the field. There is no need to reinvent the wheel when it comes to writing a powerful research hypothesis. As you’re reading and preparing your hypothesis, you’ll also read other hypotheses. These can help guide you on what works, and what doesn’t, when it comes to writing a strong research hypothesis.

Here are a few generic examples to get you started.

Eating an apple each day, after the age of 60, will result in a reduction of frequency of physician visits.

Budget airlines are more likely to receive more customer complaints. A budget airline is defined as an airline that offers lower fares and fewer amenities than a traditional full-service airline. (Note that the term “budget airline” is included in the hypothesis.

Workplaces that offer flexible working hours report higher levels of employee job satisfaction than workplaces with fixed hours.

Each of the above examples are specific, observable and measurable, and the statement of prediction can be verified or shown to be false by utilizing standard experimental practices. It should be noted, however, that often your hypothesis will change as your research progresses.

Language Editing Plus

Elsevier’s Language Editing Plus service can help ensure that your research hypothesis is well-designed, and articulates your research and conclusions. Our most comprehensive editing package, you can count on a thorough language review by native-English speakers who are PhDs or PhD candidates. We’ll check for effective logic and flow of your manuscript, as well as document formatting for your chosen journal, reference checks, and much more.

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How to Develop a Good Research Hypothesis

The story of a research study begins by asking a question. Researchers all around the globe are asking curious questions and formulating research hypothesis. However, whether the research study provides an effective conclusion depends on how well one develops a good research hypothesis. Research hypothesis examples could help researchers get an idea as to how to write a good research hypothesis.

This blog will help you understand what is a research hypothesis, its characteristics and, how to formulate a research hypothesis

Table of Contents

What is Hypothesis?

Hypothesis is an assumption or an idea proposed for the sake of argument so that it can be tested. It is a precise, testable statement of what the researchers predict will be outcome of the study.  Hypothesis usually involves proposing a relationship between two variables: the independent variable (what the researchers change) and the dependent variable (what the research measures).

What is a Research Hypothesis?

Research hypothesis is a statement that introduces a research question and proposes an expected result. It is an integral part of the scientific method that forms the basis of scientific experiments. Therefore, you need to be careful and thorough when building your research hypothesis. A minor flaw in the construction of your hypothesis could have an adverse effect on your experiment. In research, there is a convention that the hypothesis is written in two forms, the null hypothesis, and the alternative hypothesis (called the experimental hypothesis when the method of investigation is an experiment).

Characteristics of a Good Research Hypothesis

As the hypothesis is specific, there is a testable prediction about what you expect to happen in a study. You may consider drawing hypothesis from previously published research based on the theory.

A good research hypothesis involves more effort than just a guess. In particular, your hypothesis may begin with a question that could be further explored through background research.

To help you formulate a promising research hypothesis, you should ask yourself the following questions:

• Is the language clear and focused?
• What is the relationship between your hypothesis and your research topic?
• Is your hypothesis testable? If yes, then how?
• What are the possible explanations that you might want to explore?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate your variables without hampering the ethical standards?
• Does your research predict the relationship and outcome?
• Is your research simple and concise (avoids wordiness)?
• Is it clear with no ambiguity or assumptions about the readers’ knowledge
• Is your research observable and testable results?
• Is it relevant and specific to the research question or problem?

The questions listed above can be used as a checklist to make sure your hypothesis is based on a solid foundation. Furthermore, it can help you identify weaknesses in your hypothesis and revise it if necessary.

Source: Educational Hub

How to formulate a research hypothesis.

A testable hypothesis is not a simple statement. It is rather an intricate statement that needs to offer a clear introduction to a scientific experiment, its intentions, and the possible outcomes. However, there are some important things to consider when building a compelling hypothesis.

1. State the problem that you are trying to solve.

Make sure that the hypothesis clearly defines the topic and the focus of the experiment.

2. Try to write the hypothesis as an if-then statement.

Follow this template: If a specific action is taken, then a certain outcome is expected.

3. Define the variables

Independent variables are the ones that are manipulated, controlled, or changed. Independent variables are isolated from other factors of the study.

Dependent variables , as the name suggests are dependent on other factors of the study. They are influenced by the change in independent variable.

4. Scrutinize the hypothesis

Evaluate assumptions, predictions, and evidence rigorously to refine your understanding.

Types of Research Hypothesis

The types of research hypothesis are stated below:

1. Simple Hypothesis

It predicts the relationship between a single dependent variable and a single independent variable.

2. Complex Hypothesis

It predicts the relationship between two or more independent and dependent variables.

3. Directional Hypothesis

It specifies the expected direction to be followed to determine the relationship between variables and is derived from theory. Furthermore, it implies the researcher’s intellectual commitment to a particular outcome.

4. Non-directional Hypothesis

It does not predict the exact direction or nature of the relationship between the two variables. The non-directional hypothesis is used when there is no theory involved or when findings contradict previous research.

5. Associative and Causal Hypothesis

The associative hypothesis defines interdependency between variables. A change in one variable results in the change of the other variable. On the other hand, the causal hypothesis proposes an effect on the dependent due to manipulation of the independent variable.

6. Null Hypothesis

Null hypothesis states a negative statement to support the researcher’s findings that there is no relationship between two variables. There will be no changes in the dependent variable due the manipulation of the independent variable. Furthermore, it states results are due to chance and are not significant in terms of supporting the idea being investigated.

7. Alternative Hypothesis

It states that there is a relationship between the two variables of the study and that the results are significant to the research topic. An experimental hypothesis predicts what changes will take place in the dependent variable when the independent variable is manipulated. Also, it states that the results are not due to chance and that they are significant in terms of supporting the theory being investigated.

Research Hypothesis Examples of Independent and Dependent Variables

Research Hypothesis Example 1 The greater number of coal plants in a region (independent variable) increases water pollution (dependent variable). If you change the independent variable (building more coal factories), it will change the dependent variable (amount of water pollution).

Research Hypothesis Example 2 What is the effect of diet or regular soda (independent variable) on blood sugar levels (dependent variable)? If you change the independent variable (the type of soda you consume), it will change the dependent variable (blood sugar levels)

You should not ignore the importance of the above steps. The validity of your experiment and its results rely on a robust testable hypothesis. Developing a strong testable hypothesis has few advantages, it compels us to think intensely and specifically about the outcomes of a study. Consequently, it enables us to understand the implication of the question and the different variables involved in the study. Furthermore, it helps us to make precise predictions based on prior research. Hence, forming a hypothesis would be of great value to the research. Here are some good examples of testable hypotheses.

More importantly, you need to build a robust testable research hypothesis for your scientific experiments. A testable hypothesis is a hypothesis that can be proved or disproved as a result of experimentation.

Importance of a Testable Hypothesis

To devise and perform an experiment using scientific method, you need to make sure that your hypothesis is testable. To be considered testable, some essential criteria must be met:

• There must be a possibility to prove that the hypothesis is true.
• There must be a possibility to prove that the hypothesis is false.
• The results of the hypothesis must be reproducible.

Without these criteria, the hypothesis and the results will be vague. As a result, the experiment will not prove or disprove anything significant.

What are your experiences with building hypotheses for scientific experiments? What challenges did you face? How did you overcome these challenges? Please share your thoughts with us in the comments section.

Frequently Asked Questions

The steps to write a research hypothesis are: 1. Stating the problem: Ensure that the hypothesis defines the research problem 2. Writing a hypothesis as an 'if-then' statement: Include the action and the expected outcome of your study by following a ‘if-then’ structure. 3. Defining the variables: Define the variables as Dependent or Independent based on their dependency to other factors. 4. Scrutinizing the hypothesis: Identify the type of your hypothesis

Hypothesis testing is a statistical tool which is used to make inferences about a population data to draw conclusions for a particular hypothesis.

Hypothesis in statistics is a formal statement about the nature of a population within a structured framework of a statistical model. It is used to test an existing hypothesis by studying a population.

Research hypothesis is a statement that introduces a research question and proposes an expected result. It forms the basis of scientific experiments.

The different types of hypothesis in research are: • Null hypothesis: Null hypothesis is a negative statement to support the researcher’s findings that there is no relationship between two variables. • Alternate hypothesis: Alternate hypothesis predicts the relationship between the two variables of the study. • Directional hypothesis: Directional hypothesis specifies the expected direction to be followed to determine the relationship between variables. • Non-directional hypothesis: Non-directional hypothesis does not predict the exact direction or nature of the relationship between the two variables. • Simple hypothesis: Simple hypothesis predicts the relationship between a single dependent variable and a single independent variable. • Complex hypothesis: Complex hypothesis predicts the relationship between two or more independent and dependent variables. • Associative and casual hypothesis: Associative and casual hypothesis predicts the relationship between two or more independent and dependent variables. • Empirical hypothesis: Empirical hypothesis can be tested via experiments and observation. • Statistical hypothesis: A statistical hypothesis utilizes statistical models to draw conclusions about broader populations.

Wow! You really simplified your explanation that even dummies would find it easy to comprehend. Thank you so much.

Thanks a lot for your valuable guidance.

I enjoy reading the post. Hypotheses are actually an intrinsic part in a study. It bridges the research question and the methodology of the study.

Useful piece!

This is awesome.Wow.

It very interesting to read the topic, can you guide me any specific example of hypothesis process establish throw the Demand and supply of the specific product in market

Nicely explained

It is really a useful for me Kindly give some examples of hypothesis

It was a well explained content ,can you please give me an example with the null and alternative hypothesis illustrated

clear and concise. thanks.

So Good so Amazing

Good to learn

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Explained well and in simple terms. Quick read! Thank you

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Research Hypothesis: Elements, Format, Types

When a proposition is formulated for empirical testing, we call it a hypothesis. Almost all studies begin with one or more hypotheses.

Let’s Understand Research Hypothesis.

What is a hypothesis.

A hypothesis, specifically a research hypothesis, is formulated to predict an assumed relationship between two or more variables of interest.

If we reasonably guess that a relationship exists between the variables of interest, we first state it as a hypothesis and then test it in the field.

Hypotheses are stated in terms of the particular dependent and independent variables that are going to be used in the study.

Research Hypothesis Definition

A research hypothesis is a conjectural statement, a logical supposition, a reasonable guess, and an educated prediction about the nature of the relationship between two or more variables that we expect to happen in our study.

Unless you are creating an exploratory study, your hypothesis should always explain what you expect to happen during your experiment or research.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the research aims to determine whether this guess is right or wrong.

When experimenting, researchers might explore different factors to determine which ones might contribute to the outcome.

In many cases, researchers may find that the results of an experiment do not support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

Elements of a Good Hypothesis

Regardless of the type of hypothesis, the goal of a good hypothesis is to help explain the focus and direction of the experiment or research. As such, a good hypothesis will

• State the purpose of the research.
• Identify which variables are to be used.

A good hypothesis;

• Needs to be logical.
• Must be precise in language.
• It should be testable with research or experimentation.

A hypothesis is usually written in a form where it proposes that if something is done, then something will occur.

Finally, when you are trying to come up with a good hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on any previous research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research on your topic.

Once you have completed a literature review, start thinking of potential questions you still have. Pay attention to the discussion section in the journal articles you read. Many authors will suggest questions that still need to be explored.

Basic Format of a Good Hypothesis

A hypothesis often follows a basic format of “If {this happens}, then {this will happen}.” One way to structure your hypothesis is to describe what will happen to the dependent variable if you change the independent variable.

The basic format might be:

“If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}.”

A few examples:

• Students who eat breakfast will perform better on a math test than students who do not eat breakfast.
• Students who experience test anxiety before an exam get higher scores than students who do not experience test anxiety.
• Drivers who talk on their mobile phones while driving will be more likely to make errors when driving than those who do not talk on the phone.
• People with high exposure to ultraviolet light will have a higher frequency of skin cancer than those who do not have such exposure.

Look at the last example.

Here is the independent variable (exposure to ultraviolet light)) is specified, and the dependent variable (skin cancer) is also specified.

Notice also that this research hypothesis specifies a direction in that it predicts that people exposed to ultraviolet light will have a higher risk of cancer.

This is not always the case. Research hypotheses can also specify a difference without saying which group will be better or higher than the other.

For example, one might formulate a hypothesis of the type: ‘Religion does not make any significant difference in the performance of cultural activities.’

In general, however, it is considered a better hypothesis if you can specify a direction.

Research hypotheses serve several important functions. The most important one is to direct and guide the research.

A few of the other functions of the research hypothesis are enumerated below:

• A research hypothesis indicates the major independent variables to be included in the study;
• A research hypothesis suggests the type of data that must be collected and the type of analysis that must be conducted to measure the relationship;
• A research hypothesis identifies facts that are relevant and that are not;
• A research hypothesis suggests the type of research design to be employed.

Types of Research Hypothesis

Two types of research hypotheses are;

• Descriptive hypothesis.
• Relational hypothesis.

Descriptive Hypotheses

Descriptive hypotheses are propositions that typically state some variables’ existence, size, form, or distribution.

These hypotheses are formulated in the form of statements in which we assign variables to cases.

For example,

• The prevalence of contraceptive use among currently married women in India exceeds 60%.

In this example, the case is ‘currently married women,’ and the variable is ‘prevalence of contraceptives.’ As a second example,

• The public universities are currently experiencing budget difficulties.

Here,’ public universities’ is the case, and ‘budget difficulties’ is the variable.

• The National Board of Revenue claims that over 15% of potential taxpayers falsify in their income tax returns.
• At most, 75% of the pre-school children in community A have a protein-deficient diet.
• The average sales in a superstore exceed taka 25 lac per month.
• Smoking increases the risk of lung cancer.
• The average longevity of women is higher among females than among males.
• Gainfully employed women tend to have lower than average fertility.
• Women with child loss experience will have higher fertility than those who do not have such experiences.

All examples of descriptive hypotheses.

It is important to note that the Descriptive hypothesis does not always have variables that can be designated as independent or dependent.

Relational Hypotheses

Relational hypotheses, on the other hand, are statements that describe the relationship between variables concerning some cases.

• Communities with many modern facilities will have a higher rate of contraception than communities with few modern facilities.

In this instance, the case is ‘communities,’ and the variables are ‘rate of contraception’ and ‘modern facilities.’

Similarly, “People who use chewing tobacco have a higher risk of oral carcinoma than people who have never used chewing tobacco” is a relational hypothesis.

A relational hypothesis is again of two types: correlational hypothesis and the causal hypothesis.

A correlational hypothesis states that variables occur in some predictable relationships without implying that one variable causes the other to change or take on different values.

Here is an example of a co-relational hypothesis:

• Males are more efficient than their female counterparts in typing.

In making such a statement, we do not claim that sex (male-female) as a variable influences the other variable,’ typing efficiency’ (less efficient-more efficient). Here is one more example of a correlational hypothesis:

• Saving habit is more pronounced among Christians than the people of other religions.

Once again, religion is not believed to be a factor in saving habits, although a positive relationship has been observed.

Look at the following example:

• The participation of women in household decision making increases with age, their level of education, and the number of surviving children.

Here too, women’s education, several surviving children, or education does not guarantee their decision-making autonomy.

With causal hypotheses (also called explanatory hypotheses), on the other hand, there is an implication that a change in one variable causes a change or leads to an effect on the other variable.

A causal variable is typically called an independent variable, and the other is the dependent variable. It is important to note that the term “cause” roughly means “help make happen.” So, the independent variable need not be the sole reason for the existence of or change in the dependent variable. Here are some examples of causal hypotheses:

• An increase in family income leads to an increase in the income saved.
• Exposure of mothers to mass media increases their knowledge of malnutrition among their children.
• An offer of a discount in a department store enhances the sales volume.
• Chewing tobacco increases the risk of oral carcinoma.
• Goat farming contributes to poverty alleviation of rural people.
• The utilization of child welfare clinics is the lowest in those clinics in which the clinic personnel are poorly motivated to provide preventive services.
• An increase in bank interest rate encourages the customers for increased savings.

In the above example, we have ample reasons to believe that one variable (family income and savings, misuse of credit, and farm size) has a bearing on the other variable.

We cite two more examples to illustrate the hypothesis, general objective, ultimate objective, and a few specific objectives.

General objective:

• To compare the complications of acceptors of laparoscopic sterilization and mini-laparotomy among American women.

Research hypothesis:

• The risk of complications is higher in the mini-laparotomy method of sterilization than in laparoscopic sterilization.

Specific objectives:

• To assess the complications of laparoscopic sterilization and mini-laparotomy.
• To assess service providers’ knowledge and perception regarding the complications, preferences, and convenience of the two methods.

Ultimate Objectives:

• To introduce and popularize the laparoscopic female sterilization method in the National Family Planning Program to reduce the rapid population growth rate.

In a study designed to examine the living and working conditions of the overseas migrant workers from India and the pattern of remittances from overseas migrant workers, the general objective, specific objectives, and the ultimate objective were formulated as follows:

• To examine the living and working conditions of the overseas migrant workers from India.”
• Characteristics of migrant workers by significant migration channels;
• Countries of destination;
• The occupational skill of the workers;
• Pattern and procedures of remittances;
• Impact of remittances on government revenue;
• Better utilization of remittances.

Ultimate objective:

• To suggest ways and means to minimize the differences in the policy adopted by the public and private sectors in their recruitment process in the interest of the workers;
• To ascertain the possible exploitation of the workers by the private agencies and suggest remedies for such exploitation.
• Private agencies, in most cases, exploit migrant workers.

What are the elements of a good hypothesis?

A good hypothesis should state the purpose of the research, identify which variables are to be used, be logical, precise in language, and be testable with research or experimentation.

How is a hypothesis typically structured?

A hypothesis often follows a basic format of “If {this happens}, then {this will happen}.” It proposes that if something is done, then a specific outcome will occur.

What is a Descriptive hypothesis?

Descriptive hypotheses are propositions that typically state some variables’ existence, size, form, or distribution. They are formulated in the form of statements in which variables are assigned to cases.

What distinguishes a Relational hypothesis?

Relational hypotheses describe the relationship between variables concerning some cases. They can be correlational, where variables occur in a predictable relationship without implying causation, or causal, where a change in one variable causes a change in another.

What is the difference between a correlational hypothesis and a causal hypothesis?

A correlational hypothesis states that variables occur in some predictable relationships without implying that one variable causes the other to change. A causal hypothesis, on the other hand, implies that a change in one variable causes a change or leads to an effect on the other variable.

What are the two main types of research hypotheses?

The two main types of research hypotheses are Descriptive hypothesis and Relational hypothesis

What is a hypothesis in the context of academic research?

A hypothesis is a statement about an expected relationship between variables or an explanation of an occurrence that is clear, specific, and testable.

How does a research hypothesis differ from a general hypothesis?

A research hypothesis is more specific and clear about what’s being assessed and the expected outcome. It must also be testable, meaning there should be a way to prove or disprove it.

What are the essential attributes of a good research hypothesis?

A good research hypothesis should have specificity, clarity, and testability.

Why is testability crucial for a research hypothesis?

Testability ensures that empirical research can prove or disproven the hypothesis. If a statement isn’t testable, it doesn’t qualify as a research hypothesis.

What is the null hypothesis?

The null hypothesis is the counter-proposal to the original hypothesis. It predicts that there is no relationship between the variables in question.

How can one ensure that a hypothesis is clear and specific?

A hypothesis should clearly identify the variables involved, the parties involved, and the expected relationship type, leaving no ambiguity about its intent or meaning.

Why is it essential to avoid value judgments in a research hypothesis?

Value judgments are subjective and not appropriate for a hypothesis. A research hypothesis should strive to be objective, avoiding personal opinions.

What is the basic definition of a hypothesis in research?

A research hypothesis is a statement about an expected relationship between variables, or an explanation of an occurrence, that is clear, specific, and testable.

While a general hypothesis is an idea or explanation based on known facts but not yet proven, a research hypothesis is a clear, specific, and testable statement about the expected outcome of a study.

What are the essential characteristics of a good research hypothesis?

A good research hypothesis should possess specificity, clarity, and testability. It should clearly define what’s being assessed and the expected outcome, and it must be possible to prove or disprove the statement through experimentation.

How can one ensure that a hypothesis is testable?

A hypothesis is testable if there’s a possibility to prove both its truth and falsity. The results of the hypothesis should be reproducible, and it should be specific enough to allow for clear testing procedures.

What is the difference between a null hypothesis and an alternative hypothesis?

The null hypothesis proposes that no statistical significance exists in a set of observations, suggesting any differences are due to chance alone. The alternative hypothesis, on the other hand, predicts a relationship between the variables of the study and states that the results are significant to the research topic.

How should one formulate an effective research hypothesis?

To formulate an effective research hypothesis, one should state the problem clearly, use an ‘if-then’ statement structure, define the variables as dependent or independent, and scrutinize the hypothesis to ensure it meets the criteria of specificity, clarity, and testability.

What are some types of hypotheses in research?

Types of hypotheses include simple, complex, directional, non-directional, associative and causal, empirical, and statistical hypotheses. Each type serves a specific purpose and is used based on the nature of the research question or problem.

The Research Hypothesis: Role and Construction

• First Online: 01 January 2012

Cite this chapter

• Phyllis G. Supino EdD 3  

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A hypothesis is a logical construct, interposed between a problem and its solution, which represents a proposed answer to a research question. It gives direction to the investigator’s thinking about the problem and, therefore, facilitates a solution. There are three primary modes of inference by which hypotheses are developed: deduction (reasoning from a general propositions to specific instances), induction (reasoning from specific instances to a general proposition), and abduction (formulation/acceptance on probation of a hypothesis to explain a surprising observation).

A research hypothesis should reflect an inference about variables; be stated as a grammatically complete, declarative sentence; be expressed simply and unambiguously; provide an adequate answer to the research problem; and be testable. Hypotheses can be classified as conceptual versus operational, single versus bi- or multivariable, causal or not causal, mechanistic versus nonmechanistic, and null or alternative. Hypotheses most commonly entail statements about “variables” which, in turn, can be classified according to their level of measurement (scaling characteristics) or according to their role in the hypothesis (independent, dependent, moderator, control, or intervening).

A hypothesis is rendered operational when its broadly (conceptually) stated variables are replaced by operational definitions of those variables. Hypotheses stated in this manner are called operational hypotheses, specific hypotheses, or predictions and facilitate testing.

Wrong hypotheses, rightly worked from, have produced more results than unguided observation

—Augustus De Morgan, 1872[ 1 ]—

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Supino, P.G. (2012). The Research Hypothesis: Role and Construction. In: Supino, P., Borer, J. (eds) Principles of Research Methodology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-3360-6_3

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Research questions, hypotheses and objectives

Patricia farrugia.

* Michael G. DeGroote School of Medicine, the

Bradley A. Petrisor

† Division of Orthopaedic Surgery and the

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‡ Departments of Surgery and

§ Clinical Epidemiology and Biostatistics, McMaster University, Hamilton, Ont

Mohit Bhandari

There is an increasing familiarity with the principles of evidence-based medicine in the surgical community. As surgeons become more aware of the hierarchy of evidence, grades of recommendations and the principles of critical appraisal, they develop an increasing familiarity with research design. Surgeons and clinicians are looking more and more to the literature and clinical trials to guide their practice; as such, it is becoming a responsibility of the clinical research community to attempt to answer questions that are not only well thought out but also clinically relevant. The development of the research question, including a supportive hypothesis and objectives, is a necessary key step in producing clinically relevant results to be used in evidence-based practice. A well-defined and specific research question is more likely to help guide us in making decisions about study design and population and subsequently what data will be collected and analyzed. 1

Objectives of this article

In this article, we discuss important considerations in the development of a research question and hypothesis and in defining objectives for research. By the end of this article, the reader will be able to appreciate the significance of constructing a good research question and developing hypotheses and research objectives for the successful design of a research study. The following article is divided into 3 sections: research question, research hypothesis and research objectives.

Research question

Interest in a particular topic usually begins the research process, but it is the familiarity with the subject that helps define an appropriate research question for a study. 1 Questions then arise out of a perceived knowledge deficit within a subject area or field of study. 2 Indeed, Haynes suggests that it is important to know “where the boundary between current knowledge and ignorance lies.” 1 The challenge in developing an appropriate research question is in determining which clinical uncertainties could or should be studied and also rationalizing the need for their investigation.

Increasing one’s knowledge about the subject of interest can be accomplished in many ways. Appropriate methods include systematically searching the literature, in-depth interviews and focus groups with patients (and proxies) and interviews with experts in the field. In addition, awareness of current trends and technological advances can assist with the development of research questions. 2 It is imperative to understand what has been studied about a topic to date in order to further the knowledge that has been previously gathered on a topic. Indeed, some granting institutions (e.g., Canadian Institute for Health Research) encourage applicants to conduct a systematic review of the available evidence if a recent review does not already exist and preferably a pilot or feasibility study before applying for a grant for a full trial.

In-depth knowledge about a subject may generate a number of questions. It then becomes necessary to ask whether these questions can be answered through one study or if more than one study needed. 1 Additional research questions can be developed, but several basic principles should be taken into consideration. 1 All questions, primary and secondary, should be developed at the beginning and planning stages of a study. Any additional questions should never compromise the primary question because it is the primary research question that forms the basis of the hypothesis and study objectives. It must be kept in mind that within the scope of one study, the presence of a number of research questions will affect and potentially increase the complexity of both the study design and subsequent statistical analyses, not to mention the actual feasibility of answering every question. 1 A sensible strategy is to establish a single primary research question around which to focus the study plan. 3 In a study, the primary research question should be clearly stated at the end of the introduction of the grant proposal, and it usually specifies the population to be studied, the intervention to be implemented and other circumstantial factors. 4

Hulley and colleagues 2 have suggested the use of the FINER criteria in the development of a good research question ( Box 1 ). The FINER criteria highlight useful points that may increase the chances of developing a successful research project. A good research question should specify the population of interest, be of interest to the scientific community and potentially to the public, have clinical relevance and further current knowledge in the field (and of course be compliant with the standards of ethical boards and national research standards).

FINER criteria for a good research question

Adapted with permission from Wolters Kluwer Health. 2

Whereas the FINER criteria outline the important aspects of the question in general, a useful format to use in the development of a specific research question is the PICO format — consider the population (P) of interest, the intervention (I) being studied, the comparison (C) group (or to what is the intervention being compared) and the outcome of interest (O). 3 , 5 , 6 Often timing (T) is added to PICO ( Box 2 ) — that is, “Over what time frame will the study take place?” 1 The PICOT approach helps generate a question that aids in constructing the framework of the study and subsequently in protocol development by alluding to the inclusion and exclusion criteria and identifying the groups of patients to be included. Knowing the specific population of interest, intervention (and comparator) and outcome of interest may also help the researcher identify an appropriate outcome measurement tool. 7 The more defined the population of interest, and thus the more stringent the inclusion and exclusion criteria, the greater the effect on the interpretation and subsequent applicability and generalizability of the research findings. 1 , 2 A restricted study population (and exclusion criteria) may limit bias and increase the internal validity of the study; however, this approach will limit external validity of the study and, thus, the generalizability of the findings to the practical clinical setting. Conversely, a broadly defined study population and inclusion criteria may be representative of practical clinical practice but may increase bias and reduce the internal validity of the study.

PICOT criteria 1

A poorly devised research question may affect the choice of study design, potentially lead to futile situations and, thus, hamper the chance of determining anything of clinical significance, which will then affect the potential for publication. Without devoting appropriate resources to developing the research question, the quality of the study and subsequent results may be compromised. During the initial stages of any research study, it is therefore imperative to formulate a research question that is both clinically relevant and answerable.

Research hypothesis

The primary research question should be driven by the hypothesis rather than the data. 1 , 2 That is, the research question and hypothesis should be developed before the start of the study. This sounds intuitive; however, if we take, for example, a database of information, it is potentially possible to perform multiple statistical comparisons of groups within the database to find a statistically significant association. This could then lead one to work backward from the data and develop the “question.” This is counterintuitive to the process because the question is asked specifically to then find the answer, thus collecting data along the way (i.e., in a prospective manner). Multiple statistical testing of associations from data previously collected could potentially lead to spuriously positive findings of association through chance alone. 2 Therefore, a good hypothesis must be based on a good research question at the start of a trial and, indeed, drive data collection for the study.

The research or clinical hypothesis is developed from the research question and then the main elements of the study — sampling strategy, intervention (if applicable), comparison and outcome variables — are summarized in a form that establishes the basis for testing, statistical and ultimately clinical significance. 3 For example, in a research study comparing computer-assisted acetabular component insertion versus freehand acetabular component placement in patients in need of total hip arthroplasty, the experimental group would be computer-assisted insertion and the control/conventional group would be free-hand placement. The investigative team would first state a research hypothesis. This could be expressed as a single outcome (e.g., computer-assisted acetabular component placement leads to improved functional outcome) or potentially as a complex/composite outcome; that is, more than one outcome (e.g., computer-assisted acetabular component placement leads to both improved radiographic cup placement and improved functional outcome).

However, when formally testing statistical significance, the hypothesis should be stated as a “null” hypothesis. 2 The purpose of hypothesis testing is to make an inference about the population of interest on the basis of a random sample taken from that population. The null hypothesis for the preceding research hypothesis then would be that there is no difference in mean functional outcome between the computer-assisted insertion and free-hand placement techniques. After forming the null hypothesis, the researchers would form an alternate hypothesis stating the nature of the difference, if it should appear. The alternate hypothesis would be that there is a difference in mean functional outcome between these techniques. At the end of the study, the null hypothesis is then tested statistically. If the findings of the study are not statistically significant (i.e., there is no difference in functional outcome between the groups in a statistical sense), we cannot reject the null hypothesis, whereas if the findings were significant, we can reject the null hypothesis and accept the alternate hypothesis (i.e., there is a difference in mean functional outcome between the study groups), errors in testing notwithstanding. In other words, hypothesis testing confirms or refutes the statement that the observed findings did not occur by chance alone but rather occurred because there was a true difference in outcomes between these surgical procedures. The concept of statistical hypothesis testing is complex, and the details are beyond the scope of this article.

Another important concept inherent in hypothesis testing is whether the hypotheses will be 1-sided or 2-sided. A 2-sided hypothesis states that there is a difference between the experimental group and the control group, but it does not specify in advance the expected direction of the difference. For example, we asked whether there is there an improvement in outcomes with computer-assisted surgery or whether the outcomes worse with computer-assisted surgery. We presented a 2-sided test in the above example because we did not specify the direction of the difference. A 1-sided hypothesis states a specific direction (e.g., there is an improvement in outcomes with computer-assisted surgery). A 2-sided hypothesis should be used unless there is a good justification for using a 1-sided hypothesis. As Bland and Atlman 8 stated, “One-sided hypothesis testing should never be used as a device to make a conventionally nonsignificant difference significant.”

The research hypothesis should be stated at the beginning of the study to guide the objectives for research. Whereas the investigators may state the hypothesis as being 1-sided (there is an improvement with treatment), the study and investigators must adhere to the concept of clinical equipoise. According to this principle, a clinical (or surgical) trial is ethical only if the expert community is uncertain about the relative therapeutic merits of the experimental and control groups being evaluated. 9 It means there must exist an honest and professional disagreement among expert clinicians about the preferred treatment. 9

Designing a research hypothesis is supported by a good research question and will influence the type of research design for the study. Acting on the principles of appropriate hypothesis development, the study can then confidently proceed to the development of the research objective.

Research objective

The primary objective should be coupled with the hypothesis of the study. Study objectives define the specific aims of the study and should be clearly stated in the introduction of the research protocol. 7 From our previous example and using the investigative hypothesis that there is a difference in functional outcomes between computer-assisted acetabular component placement and free-hand placement, the primary objective can be stated as follows: this study will compare the functional outcomes of computer-assisted acetabular component insertion versus free-hand placement in patients undergoing total hip arthroplasty. Note that the study objective is an active statement about how the study is going to answer the specific research question. Objectives can (and often do) state exactly which outcome measures are going to be used within their statements. They are important because they not only help guide the development of the protocol and design of study but also play a role in sample size calculations and determining the power of the study. 7 These concepts will be discussed in other articles in this series.

From the surgeon’s point of view, it is important for the study objectives to be focused on outcomes that are important to patients and clinically relevant. For example, the most methodologically sound randomized controlled trial comparing 2 techniques of distal radial fixation would have little or no clinical impact if the primary objective was to determine the effect of treatment A as compared to treatment B on intraoperative fluoroscopy time. However, if the objective was to determine the effect of treatment A as compared to treatment B on patient functional outcome at 1 year, this would have a much more significant impact on clinical decision-making. Second, more meaningful surgeon–patient discussions could ensue, incorporating patient values and preferences with the results from this study. 6 , 7 It is the precise objective and what the investigator is trying to measure that is of clinical relevance in the practical setting.

The following is an example from the literature about the relation between the research question, hypothesis and study objectives:

Study: Warden SJ, Metcalf BR, Kiss ZS, et al. Low-intensity pulsed ultrasound for chronic patellar tendinopathy: a randomized, double-blind, placebo-controlled trial. Rheumatology 2008;47:467–71.

Research question: How does low-intensity pulsed ultrasound (LIPUS) compare with a placebo device in managing the symptoms of skeletally mature patients with patellar tendinopathy?

Research hypothesis: Pain levels are reduced in patients who receive daily active-LIPUS (treatment) for 12 weeks compared with individuals who receive inactive-LIPUS (placebo).

Objective: To investigate the clinical efficacy of LIPUS in the management of patellar tendinopathy symptoms.

The development of the research question is the most important aspect of a research project. A research project can fail if the objectives and hypothesis are poorly focused and underdeveloped. Useful tips for surgical researchers are provided in Box 3 . Designing and developing an appropriate and relevant research question, hypothesis and objectives can be a difficult task. The critical appraisal of the research question used in a study is vital to the application of the findings to clinical practice. Focusing resources, time and dedication to these 3 very important tasks will help to guide a successful research project, influence interpretation of the results and affect future publication efforts.

Tips for developing research questions, hypotheses and objectives for research studies

• Perform a systematic literature review (if one has not been done) to increase knowledge and familiarity with the topic and to assist with research development.
• Learn about current trends and technological advances on the topic.
• Seek careful input from experts, mentors, colleagues and collaborators to refine your research question as this will aid in developing the research question and guide the research study.
• Use the FINER criteria in the development of the research question.
• Ensure that the research question follows PICOT format.
• Develop a research hypothesis from the research question.
• Develop clear and well-defined primary and secondary (if needed) objectives.
• Ensure that the research question and objectives are answerable, feasible and clinically relevant.

FINER = feasible, interesting, novel, ethical, relevant; PICOT = population (patients), intervention (for intervention studies only), comparison group, outcome of interest, time.

Competing interests: No funding was received in preparation of this paper. Dr. Bhandari was funded, in part, by a Canada Research Chair, McMaster University.

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Definition of hypothesis

Did you know.

The Difference Between Hypothesis and Theory

A hypothesis is an assumption, an idea that is proposed for the sake of argument so that it can be tested to see if it might be true.

In the scientific method, the hypothesis is constructed before any applicable research has been done, apart from a basic background review. You ask a question, read up on what has been studied before, and then form a hypothesis.

A hypothesis is usually tentative; it's an assumption or suggestion made strictly for the objective of being tested.

A theory , in contrast, is a principle that has been formed as an attempt to explain things that have already been substantiated by data. It is used in the names of a number of principles accepted in the scientific community, such as the Big Bang Theory . Because of the rigors of experimentation and control, it is understood to be more likely to be true than a hypothesis is.

In non-scientific use, however, hypothesis and theory are often used interchangeably to mean simply an idea, speculation, or hunch, with theory being the more common choice.

Since this casual use does away with the distinctions upheld by the scientific community, hypothesis and theory are prone to being wrongly interpreted even when they are encountered in scientific contexts—or at least, contexts that allude to scientific study without making the critical distinction that scientists employ when weighing hypotheses and theories.

The most common occurrence is when theory is interpreted—and sometimes even gleefully seized upon—to mean something having less truth value than other scientific principles. (The word law applies to principles so firmly established that they are almost never questioned, such as the law of gravity.)

This mistake is one of projection: since we use theory in general to mean something lightly speculated, then it's implied that scientists must be talking about the same level of uncertainty when they use theory to refer to their well-tested and reasoned principles.

The distinction has come to the forefront particularly on occasions when the content of science curricula in schools has been challenged—notably, when a school board in Georgia put stickers on textbooks stating that evolution was "a theory, not a fact, regarding the origin of living things." As Kenneth R. Miller, a cell biologist at Brown University, has said , a theory "doesn’t mean a hunch or a guess. A theory is a system of explanations that ties together a whole bunch of facts. It not only explains those facts, but predicts what you ought to find from other observations and experiments.”

While theories are never completely infallible, they form the basis of scientific reasoning because, as Miller said "to the best of our ability, we’ve tested them, and they’ve held up."

• proposition
• supposition

hypothesis , theory , law mean a formula derived by inference from scientific data that explains a principle operating in nature.

hypothesis implies insufficient evidence to provide more than a tentative explanation.

theory implies a greater range of evidence and greater likelihood of truth.

law implies a statement of order and relation in nature that has been found to be invariable under the same conditions.

Examples of hypothesis in a Sentence

These examples are programmatically compiled from various online sources to illustrate current usage of the word 'hypothesis.' Any opinions expressed in the examples do not represent those of Merriam-Webster or its editors. Send us feedback about these examples.

Word History

Greek, from hypotithenai to put under, suppose, from hypo- + tithenai to put — more at do

1641, in the meaning defined at sense 1a

Phrases Containing hypothesis

• counter - hypothesis
• nebular hypothesis
• null hypothesis
• planetesimal hypothesis
• Whorfian hypothesis

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“Hypothesis.” Merriam-Webster.com Dictionary , Merriam-Webster, https://www.merriam-webster.com/dictionary/hypothesis. Accessed 7 Jun. 2024.

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Hypothesis is a testable statement that explains what is happening or observed. It proposes the relation between the various participating variables. Hypothesis is also called Theory, Thesis, Guess, Assumption, or Suggestion. Hypothesis creates a structure that guides the search for knowledge.

In this article, we will learn what is hypothesis, its characteristics, types, and examples. We will also learn how hypothesis helps in scientific research.

Table of Content

What is Hypothesis?

Hypothesis meaning, characteristics of hypothesis, sources of hypothesis, types of hypothesis, simple hypothesis, complex hypothesis, directional hypothesis, non-directional hypothesis, null hypothesis (h0), alternative hypothesis (h1 or ha), statistical hypothesis, research hypothesis, associative hypothesis, causal hypothesis, hypothesis examples, simple hypothesis example, complex hypothesis example, directional hypothesis example, non-directional hypothesis example, alternative hypothesis (ha), functions of hypothesis, how hypothesis help in scientific research.

A hypothesis is a suggested idea or plan that has little proof, meant to lead to more study. It’s mainly a smart guess or suggested answer to a problem that can be checked through study and trial. In science work, we make guesses called hypotheses to try and figure out what will happen in tests or watching. These are not sure things but rather ideas that can be proved or disproved based on real-life proofs. A good theory is clear and can be tested and found wrong if the proof doesn’t support it.

A hypothesis is a proposed statement that is testable and is given for something that happens or observed.

• It is made using what we already know and have seen, and it’s the basis for scientific research.
• A clear guess tells us what we think will happen in an experiment or study.
• It’s a testable clue that can be proven true or wrong with real-life facts and checking it out carefully.
• It usually looks like a “if-then” rule, showing the expected cause and effect relationship between what’s being studied.

Here are some key characteristics of a hypothesis:

• Testable: An idea (hypothesis) should be made so it can be tested and proven true through doing experiments or watching. It should show a clear connection between things.
• Specific: It needs to be easy and on target, talking about a certain part or connection between things in a study.
• Falsifiable: A good guess should be able to show it’s wrong. This means there must be a chance for proof or seeing something that goes against the guess.
• Logical and Rational: It should be based on things we know now or have seen, giving a reasonable reason that fits with what we already know.
• Predictive: A guess often tells what to expect from an experiment or observation. It gives a guide for what someone might see if the guess is right.
• Concise: It should be short and clear, showing the suggested link or explanation simply without extra confusion.
• Grounded in Research: A guess is usually made from before studies, ideas or watching things. It comes from a deep understanding of what is already known in that area.
• Flexible: A guess helps in the research but it needs to change or fix when new information comes up.
• Relevant: It should be related to the question or problem being studied, helping to direct what the research is about.
• Empirical: Hypotheses come from observations and can be tested using methods based on real-world experiences.

Hypotheses can come from different places based on what you’re studying and the kind of research. Here are some common sources from which hypotheses may originate:

• Existing Theories: Often, guesses come from well-known science ideas. These ideas may show connections between things or occurrences that scientists can look into more.
• Observation and Experience: Watching something happen or having personal experiences can lead to guesses. We notice odd things or repeat events in everyday life and experiments. This can make us think of guesses called hypotheses.
• Previous Research: Using old studies or discoveries can help come up with new ideas. Scientists might try to expand or question current findings, making guesses that further study old results.
• Literature Review: Looking at books and research in a subject can help make guesses. Noticing missing parts or mismatches in previous studies might make researchers think up guesses to deal with these spots.
• Problem Statement or Research Question: Often, ideas come from questions or problems in the study. Making clear what needs to be looked into can help create ideas that tackle certain parts of the issue.
• Analogies or Comparisons: Making comparisons between similar things or finding connections from related areas can lead to theories. Understanding from other fields could create new guesses in a different situation.
• Hunches and Speculation: Sometimes, scientists might get a gut feeling or make guesses that help create ideas to test. Though these may not have proof at first, they can be a beginning for looking deeper.
• Technology and Innovations: New technology or tools might make guesses by letting us look at things that were hard to study before.
• Personal Interest and Curiosity: People’s curiosity and personal interests in a topic can help create guesses. Scientists could make guesses based on their own likes or love for a subject.

Here are some common types of hypotheses:

• Non-directional Hypothesis

Simple Hypothesis guesses a connection between two things. It says that there is a connection or difference between variables, but it doesn’t tell us which way the relationship goes.

Complex Hypothesis tells us what will happen when more than two things are connected. It looks at how different things interact and may be linked together.

Directional Hypothesis says how one thing is related to another. For example, it guesses that one thing will help or hurt another thing.

Non-Directional Hypothesis are the one that don’t say how the relationship between things will be. They just say that there is a connection, without telling which way it goes.

Null hypothesis is a statement that says there’s no connection or difference between different things. It implies that any seen impacts are because of luck or random changes in the information.

Alternative Hypothesis is different from the null hypothesis and shows that there’s a big connection or gap between variables. Scientists want to say no to the null hypothesis and choose the alternative one.

Statistical Hypotheis are used in math testing and include making ideas about what groups or bits of them look like. You aim to get information or test certain things using these top-level, common words only.

Research Hypothesis comes from the research question and tells what link is expected between things or factors. It leads the study and chooses where to look more closely.

Associative Hypotheis guesses that there is a link or connection between things without really saying it caused them. It means that when one thing changes, it is connected to another thing changing.

Causal Hypothesis are different from other ideas because they say that one thing causes another. This means there’s a cause and effect relationship between variables involved in the situation. They say that when one thing changes, it directly makes another thing change.

Following are the examples of hypotheses based on their types:

• Studying more can help you do better on tests.
• Getting more sun makes people have higher amounts of vitamin D.
• How rich you are, how easy it is to get education and healthcare greatly affects the number of years people live.
• A new medicine’s success relies on the amount used, how old a person is who takes it and their genes.
• Drinking more sweet drinks is linked to a higher body weight score.
• Too much stress makes people less productive at work.
• Drinking caffeine can affect how well you sleep.
• People often like different kinds of music based on their gender.
• The average test scores of Group A and Group B are not much different.
• There is no connection between using a certain fertilizer and how much it helps crops grow.
• Patients on Diet A have much different cholesterol levels than those following Diet B.
• Exposure to a certain type of light can change how plants grow compared to normal sunlight.
• The average smarts score of kids in a certain school area is 100.
• The usual time it takes to finish a job using Method A is the same as with Method B.
• Having more kids go to early learning classes helps them do better in school when they get older.
• Using specific ways of talking affects how much customers get involved in marketing activities.
• Regular exercise helps to lower the chances of heart disease.
• Going to school more can help people make more money.
• Playing violent video games makes teens more likely to act aggressively.
• Less clean air directly impacts breathing health in city populations.

Hypotheses have many important jobs in the process of scientific research. Here are the key functions of hypotheses:

• Guiding Research: Hypotheses give a clear and exact way for research. They act like guides, showing the predicted connections or results that scientists want to study.
• Formulating Research Questions: Research questions often create guesses. They assist in changing big questions into particular, checkable things. They guide what the study should be focused on.
• Setting Clear Objectives: Hypotheses set the goals of a study by saying what connections between variables should be found. They set the targets that scientists try to reach with their studies.
• Testing Predictions: Theories guess what will happen in experiments or observations. By doing tests in a planned way, scientists can check if what they see matches the guesses made by their ideas.
• Providing Structure: Theories give structure to the study process by arranging thoughts and ideas. They aid scientists in thinking about connections between things and plan experiments to match.
• Focusing Investigations: Hypotheses help scientists focus on certain parts of their study question by clearly saying what they expect links or results to be. This focus makes the study work better.
• Facilitating Communication: Theories help scientists talk to each other effectively. Clearly made guesses help scientists to tell others what they plan, how they will do it and the results expected. This explains things well with colleagues in a wide range of audiences.
• Generating Testable Statements: A good guess can be checked, which means it can be looked at carefully or tested by doing experiments. This feature makes sure that guesses add to the real information used in science knowledge.
• Promoting Objectivity: Guesses give a clear reason for study that helps guide the process while reducing personal bias. They motivate scientists to use facts and data as proofs or disprovals for their proposed answers.
• Driving Scientific Progress: Making, trying out and adjusting ideas is a cycle. Even if a guess is proven right or wrong, the information learned helps to grow knowledge in one specific area.

Researchers use hypotheses to put down their thoughts directing how the experiment would take place. Following are the steps that are involved in the scientific method:

• Initiating Investigations: Hypotheses are the beginning of science research. They come from watching, knowing what’s already known or asking questions. This makes scientists make certain explanations that need to be checked with tests.
• Formulating Research Questions: Ideas usually come from bigger questions in study. They help scientists make these questions more exact and testable, guiding the study’s main point.
• Setting Clear Objectives: Hypotheses set the goals of a study by stating what we think will happen between different things. They set the goals that scientists want to reach by doing their studies.
• Designing Experiments and Studies: Assumptions help plan experiments and watchful studies. They assist scientists in knowing what factors to measure, the techniques they will use and gather data for a proposed reason.
• Testing Predictions: Ideas guess what will happen in experiments or observations. By checking these guesses carefully, scientists can see if the seen results match up with what was predicted in each hypothesis.
• Analysis and Interpretation of Data: Hypotheses give us a way to study and make sense of information. Researchers look at what they found and see if it matches the guesses made in their theories. They decide if the proof backs up or disagrees with these suggested reasons why things are happening as expected.
• Encouraging Objectivity: Hypotheses help make things fair by making sure scientists use facts and information to either agree or disagree with their suggested reasons. They lessen personal preferences by needing proof from experience.
• Iterative Process: People either agree or disagree with guesses, but they still help the ongoing process of science. Findings from testing ideas make us ask new questions, improve those ideas and do more tests. It keeps going on in the work of science to keep learning things.

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Summary – Hypothesis

A hypothesis is a testable statement serving as an initial explanation for phenomena, based on observations, theories, or existing knowledge. It acts as a guiding light for scientific research, proposing potential relationships between variables that can be empirically tested through experiments and observations.

The hypothesis must be specific, testable, falsifiable, and grounded in prior research or observation, laying out a predictive, if-then scenario that details a cause-and-effect relationship. It originates from various sources including existing theories, observations, previous research, and even personal curiosity, leading to different types, such as simple, complex, directional, non-directional, null, and alternative hypotheses, each serving distinct roles in research methodology .

The hypothesis not only guides the research process by shaping objectives and designing experiments but also facilitates objective analysis and interpretation of data , ultimately driving scientific progress through a cycle of testing, validation, and refinement.

Hypothesis – FAQs

What is a hypothesis.

A guess is a possible explanation or forecast that can be checked by doing research and experiments.

What are Components of a Hypothesis?

The components of a Hypothesis are Independent Variable, Dependent Variable, Relationship between Variables, Directionality etc.

What makes a Good Hypothesis?

Testability, Falsifiability, Clarity and Precision, Relevance are some parameters that makes a Good Hypothesis

Can a Hypothesis be Proven True?

You cannot prove conclusively that most hypotheses are true because it’s generally impossible to examine all possible cases for exceptions that would disprove them.

How are Hypotheses Tested?

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data

Can Hypotheses change during Research?

Yes, you can change or improve your ideas based on new information discovered during the research process.

What is the Role of a Hypothesis in Scientific Research?

Hypotheses are used to support scientific research and bring about advancements in knowledge.

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What is Hypothesis?

We have heard of many hypotheses which have led to great inventions in science. Assumptions that are made on the basis of some evidence are known as hypotheses. In this article, let us learn in detail about the hypothesis and the type of hypothesis with examples.

A hypothesis is an assumption that is made based on some evidence. This is the initial point of any investigation that translates the research questions into predictions. It includes components like variables, population and the relation between the variables. A research hypothesis is a hypothesis that is used to test the relationship between two or more variables.

Characteristics of Hypothesis

Following are the characteristics of the hypothesis:

• The hypothesis should be clear and precise to consider it to be reliable.
• If the hypothesis is a relational hypothesis, then it should be stating the relationship between variables.
• The hypothesis must be specific and should have scope for conducting more tests.
• The way of explanation of the hypothesis must be very simple and it should also be understood that the simplicity of the hypothesis is not related to its significance.

Sources of Hypothesis

Following are the sources of hypothesis:

• The resemblance between the phenomenon.
• Observations from past studies, present-day experiences and from the competitors.
• Scientific theories.
• General patterns that influence the thinking process of people.

Types of Hypothesis

There are six forms of hypothesis and they are:

• Simple hypothesis
• Complex hypothesis
• Directional hypothesis
• Non-directional hypothesis
• Null hypothesis
• Associative and casual hypothesis

Simple Hypothesis

It shows a relationship between one dependent variable and a single independent variable. For example – If you eat more vegetables, you will lose weight faster. Here, eating more vegetables is an independent variable, while losing weight is the dependent variable.

Complex Hypothesis

It shows the relationship between two or more dependent variables and two or more independent variables. Eating more vegetables and fruits leads to weight loss, glowing skin, and reduces the risk of many diseases such as heart disease.

Directional Hypothesis

It shows how a researcher is intellectual and committed to a particular outcome. The relationship between the variables can also predict its nature. For example- children aged four years eating proper food over a five-year period are having higher IQ levels than children not having a proper meal. This shows the effect and direction of the effect.

Non-directional Hypothesis

It is used when there is no theory involved. It is a statement that a relationship exists between two variables, without predicting the exact nature (direction) of the relationship.

Null Hypothesis

It provides a statement which is contrary to the hypothesis. It’s a negative statement, and there is no relationship between independent and dependent variables. The symbol is denoted by “H O ”.

Associative and Causal Hypothesis

Associative hypothesis occurs when there is a change in one variable resulting in a change in the other variable. Whereas, the causal hypothesis proposes a cause and effect interaction between two or more variables.

Examples of Hypothesis

Following are the examples of hypotheses based on their types:

• Consumption of sugary drinks every day leads to obesity is an example of a simple hypothesis.
• All lilies have the same number of petals is an example of a null hypothesis.
• If a person gets 7 hours of sleep, then he will feel less fatigue than if he sleeps less. It is an example of a directional hypothesis.

Functions of Hypothesis

Following are the functions performed by the hypothesis:

• Hypothesis helps in making an observation and experiments possible.
• It becomes the start point for the investigation.
• Hypothesis helps in verifying the observations.
• It helps in directing the inquiries in the right direction.

How will Hypothesis help in the Scientific Method?

Researchers use hypotheses to put down their thoughts directing how the experiment would take place. Following are the steps that are involved in the scientific method:

• Formation of question
• Doing background research
• Creation of hypothesis
• Designing an experiment
• Collection of data
• Result analysis
• Summarizing the experiment
• Communicating the results

Frequently Asked Questions – FAQs

What is hypothesis.

A hypothesis is an assumption made based on some evidence.

Give an example of simple hypothesis?

What are the types of hypothesis.

Types of hypothesis are:

• Associative and Casual hypothesis

State true or false: Hypothesis is the initial point of any investigation that translates the research questions into a prediction.

Define complex hypothesis..

A complex hypothesis shows the relationship between two or more dependent variables and two or more independent variables.

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2.1.6: Steps of the Hypothesis Testing Process

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• Page ID 44861

• Michelle Oja
• Taft College

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The process of testing hypotheses follows a simple four-step procedure. This process will be what we use for the remained of the textbook and course, and though the hypothesis and statistics we use will change, this process will not.

Step 1: State the Hypotheses

Your hypotheses are the first thing you need to lay out. Otherwise, there is nothing to test! You have to state the null hypothesis (which is what we test) and the research hypothesis (which is what we expect). These should be stated mathematically as they were presented above AND in words, explaining in normal English what each one means in terms of the research question.

Step 2: Find the Critical Values

Next, we formally lay out the criteria we will use to test our hypotheses. There are two pieces of information that inform our critical values: \(α\), wh ich determines how much of the area under the curve composes our rejection region, and the directionality of the test, which determines where the region will be.

Step 3: Compute the Test Statistic

Once we have our hypotheses and the standards we use to test them, we can collect data and calculate our test statistic . This step is where the vast majority of differences in future chapters will arise: different tests used for different data are calculated in different ways, but the way we use and interpret them remains the same.

Step 4: Make the Decision

Finally, once we have our calculated test statistic, we can compare it to our critical value and decide whether we should reject or fail to reject the null hypothesis. When we do this, we must interpret the decision in relation to our research question, stating what we concluded, what we based our conclusion on, and the specific statistics we obtained.

We will talk more about what is included in the write-up that explains the interpretation of the decision in relation to the research question, but remember that your answer is never just a number in behavioral statistics. And in Null Hypothesis Significance Testing, your answer is probably at least several sentences explaining the groups, what was measured, the results. and how it all relates to the research hypothesis.

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Understanding Science

How science REALLY works...

• Understanding Science 101
• Misconceptions
• Testing ideas with evidence from the natural world is at the core of science.
• Scientific testing involves figuring out what we would  expect  to observe if an idea were correct and comparing that expectation to what we  actually  observe.
• Scientific arguments are built from an idea and the evidence relevant to that idea.
• Scientific arguments can be built in any order. Sometimes a scientific idea precedes any evidence relevant to it, and other times the evidence helps inspire the idea.

Misconception:  Science proves ideas.

Misconception:  Science can only disprove ideas.

Correction:  Science neither proves nor disproves. It accepts or rejects ideas based on supporting and refuting evidence, but may revise those conclusions if warranted by new evidence or perspectives.  Read more about it.

The core of science: Relating evidence and ideas

In this case, the term  argument  refers not to a disagreement between two people, but to an evidence-based line of reasoning — so scientific arguments are more like the closing argument in a court case (a logical description of what we think and why we think it) than they are like the fights you may have had with siblings. Scientific arguments involve three components: the idea (a  hypothesis  or theory), the  expectations  generated by that idea (frequently called predictions), and the actual observations relevant to those expectations (the evidence). These components are always related in the same logical way:

• What would we expect to see if this idea were true (i.e., what is our expected observation)?
• What do we actually observe?
• Do our expectations match our observations?

PREDICTIONS OR EXPECTATIONS?

When scientists describe their arguments, they frequently talk about their expectations in terms of what a hypothesis or theory predicts: “If it were the case that smoking causes lung cancer, then we’d  predict  that countries with higher rates of smoking would have higher rates of lung cancer.” At first, it might seem confusing to talk about a prediction that doesn’t deal with the future, but that refers to something going on right now or that may have already happened. In fact, this is just another way of discussing the expectations that the hypothesis or theory generates. So when a scientist talks about the  predicted  rates of lung cancer, he or she really means something like “the rates that we’d expect to see if our hypothesis were correct.”

If the idea generates expectations that hold true (are actually observed), then the idea is more likely to be accurate. If the idea generates expectations that don’t hold true (are not observed), then we are less likely to  accept  the idea. For example, consider the idea that cells are the building blocks of life. If that idea were true, we’d expect to see cells in all kinds of living tissues observed under a microscope — that’s our expected observation. In fact, we do observe this (our actual observation), so evidence supports the idea that living things are built from cells.

Though the structure of this argument is consistent (hypothesis, then expectation, then actual observation), its pieces may be assembled in different orders. For example, the first observations of cells were made in the 1600s, but cell theory was not postulated until 200 years later — so in this case, the evidence actually helped inspire the idea. Whether the idea comes first or the evidence comes first, the logic relating them remains the same.

Here, we’ll explore scientific arguments and how to build them. You can investigate:

Putting the pieces together: The hard work of building arguments

• Predicting the past
• Arguments with legs to stand on

Or just click the  Next  button to dive right in!

• Take a sidetrip
• Teaching resources

Scientific arguments rely on testable ideas. To learn what makes an idea testable, review our  Science Checklist .

• Forming hypotheses — scientific explanations — can be difficult for students. It is often easier for students to generate an expectation (what they think will happen or what they expect to observe) based on prior experience than to formulate a potential explanation for that phenomena. You can help students go beyond expectations to generate real, explanatory hypotheses by providing sentence stems for them to fill in: “I expect to observe A because B.” Once students have filled in this sentence you can explain that B is a hypothesis and A is the expectation generated by that hypothesis.
• You can help students learn to distinguish between hypotheses and the expectations generated by them by regularly asking students to analyze lecture material, text, or video. Students should try to figure out which aspects of the content were hypotheses and which were expectations.

Summing up the process

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Chi-Square (Χ²) Test & How To Calculate Formula Equation

Benjamin Frimodig

Science Expert

B.A., History and Science, Harvard University

Ben Frimodig is a 2021 graduate of Harvard College, where he studied the History of Science.

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Saul Mcleod, PhD

Editor-in-Chief for Simply Psychology

BSc (Hons) Psychology, MRes, PhD, University of Manchester

Saul Mcleod, PhD., is a qualified psychology teacher with over 18 years of experience in further and higher education. He has been published in peer-reviewed journals, including the Journal of Clinical Psychology.

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Chi-square (χ2) is used to test hypotheses about the distribution of observations into categories with no inherent ranking.

What Is a Chi-Square Statistic?

The Chi-square test (pronounced Kai) looks at the pattern of observations and will tell us if certain combinations of the categories occur more frequently than we would expect by chance, given the total number of times each category occurred.

It looks for an association between the variables. We cannot use a correlation coefficient to look for the patterns in this data because the categories often do not form a continuum.

There are three main types of Chi-square tests, tests of goodness of fit, the test of independence, and the test for homogeneity. All three tests rely on the same formula to compute a test statistic.

These tests function by deciphering relationships between observed sets of data and theoretical or “expected” sets of data that align with the null hypothesis.

What is a Contingency Table?

Contingency tables (also known as two-way tables) are grids in which Chi-square data is organized and displayed. They provide a basic picture of the interrelation between two variables and can help find interactions between them.

In contingency tables, one variable and each of its categories are listed vertically, and the other variable and each of its categories are listed horizontally.

Additionally, including column and row totals, also known as “marginal frequencies,” will help facilitate the Chi-square testing process.

In order for the Chi-square test to be considered trustworthy, each cell of your expected contingency table must have a value of at least five.

Each Chi-square test will have one contingency table representing observed counts (see Fig. 1) and one contingency table representing expected counts (see Fig. 2).

Figure 1. Observed table (which contains the observed counts).

To obtain the expected frequencies for any cell in any cross-tabulation in which the two variables are assumed independent, multiply the row and column totals for that cell and divide the product by the total number of cases in the table.

Figure 2. Expected table (what we expect the two-way table to look like if the two categorical variables are independent).

To decide if our calculated value for χ2 is significant, we also need to work out the degrees of freedom for our contingency table using the following formula: df= (rows – 1) x (columns – 1).

Formula Calculation

Calculate the chi-square statistic (χ2) by completing the following steps:

• Calculate the expected frequencies and the observed frequencies.
• For each observed number in the table, subtract the corresponding expected number (O — E).
• Square the difference (O —E)².
• Divide the squares obtained for each cell in the table by the expected number for that cell (O – E)² / E.
• Sum all the values for (O – E)² / E. This is the chi-square statistic.
• Calculate the degrees of freedom for the contingency table using the following formula; df= (rows – 1) x (columns – 1).

Once we have calculated the degrees of freedom (df) and the chi-squared value (χ2), we can use the χ2 table (often at the back of a statistics book) to check if our value for χ2 is higher than the critical value given in the table. If it is, then our result is significant at the level given.

Interpretation

The chi-square statistic tells you how much difference exists between the observed count in each table cell to the counts you would expect if there were no relationship at all in the population.

Small Chi-Square Statistic: If the chi-square statistic is small and the p-value is large (usually greater than 0.05), this often indicates that the observed frequencies in the sample are close to what would be expected under the null hypothesis.

The null hypothesis usually states no association between the variables being studied or that the observed distribution fits the expected distribution.

In theory, if the observed and expected values were equal (no difference), then the chi-square statistic would be zero — but this is unlikely to happen in real life.

Large Chi-Square Statistic : If the chi-square statistic is large and the p-value is small (usually less than 0.05), then the conclusion is often that the data does not fit the model well, i.e., the observed and expected values are significantly different. This often leads to the rejection of the null hypothesis.

How to Report

To report a chi-square output in an APA-style results section, always rely on the following template:

χ2 ( degrees of freedom , N = sample size ) = chi-square statistic value , p = p value .

In the case of the above example, the results would be written as follows:

A chi-square test of independence showed that there was a significant association between gender and post-graduation education plans, χ2 (4, N = 101) = 54.50, p < .001.

APA Style Rules

• Do not use a zero before a decimal when the statistic cannot be greater than 1 (proportion, correlation, level of statistical significance).
• Report exact p values to two or three decimals (e.g., p = .006, p = .03).
• However, report p values less than .001 as “ p < .001.”
• Put a space before and after a mathematical operator (e.g., minus, plus, greater than, less than, equals sign).
• Do not repeat statistics in both the text and a table or figure.

p -value Interpretation

You test whether a given χ2 is statistically significant by testing it against a table of chi-square distributions , according to the number of degrees of freedom for your sample, which is the number of categories minus 1. The chi-square assumes that you have at least 5 observations per category.

If you are using SPSS then you will have an expected p -value.

For a chi-square test, a p-value that is less than or equal to the .05 significance level indicates that the observed values are different to the expected values.

Thus, low p-values (p< .05) indicate a likely difference between the theoretical population and the collected sample. You can conclude that a relationship exists between the categorical variables.

Remember that p -values do not indicate the odds that the null hypothesis is true but rather provide the probability that one would obtain the sample distribution observed (or a more extreme distribution) if the null hypothesis was true.

A level of confidence necessary to accept the null hypothesis can never be reached. Therefore, conclusions must choose to either fail to reject the null or accept the alternative hypothesis, depending on the calculated p-value.

The four steps below show you how to analyze your data using a chi-square goodness-of-fit test in SPSS (when you have hypothesized that you have equal expected proportions).

Step 1 : Analyze > Nonparametric Tests > Legacy Dialogs > Chi-square… on the top menu as shown below:

Step 2 : Move the variable indicating categories into the “Test Variable List:” box.

Step 3 : If you want to test the hypothesis that all categories are equally likely, click “OK.”

Step 4 : Specify the expected count for each category by first clicking the “Values” button under “Expected Values.”

Step 5 : Then, in the box to the right of “Values,” enter the expected count for category one and click the “Add” button. Now enter the expected count for category two and click “Add.” Continue in this way until all expected counts have been entered.

Step 6 : Then click “OK.”

The four steps below show you how to analyze your data using a chi-square test of independence in SPSS Statistics.

Step 1 : Open the Crosstabs dialog (Analyze > Descriptive Statistics > Crosstabs).

Step 2 : Select the variables you want to compare using the chi-square test. Click one variable in the left window and then click the arrow at the top to move the variable. Select the row variable and the column variable.

Step 3 : Click Statistics (a new pop-up window will appear). Check Chi-square, then click Continue.

Step 4 : (Optional) Check the box for Display clustered bar charts.

Step 5 : Click OK.

Goodness-of-Fit Test

The Chi-square goodness of fit test is used to compare a randomly collected sample containing a single, categorical variable to a larger population.

This test is most commonly used to compare a random sample to the population from which it was potentially collected.

The test begins with the creation of a null and alternative hypothesis. In this case, the hypotheses are as follows:

Null Hypothesis (Ho) : The null hypothesis (Ho) is that the observed frequencies are the same (except for chance variation) as the expected frequencies. The collected data is consistent with the population distribution.

Alternative Hypothesis (Ha) : The collected data is not consistent with the population distribution.

The next step is to create a contingency table that represents how the data would be distributed if the null hypothesis were exactly correct.

The sample’s overall deviation from this theoretical/expected data will allow us to draw a conclusion, with a more severe deviation resulting in smaller p-values.

Test for Independence

The Chi-square test for independence looks for an association between two categorical variables within the same population.

Unlike the goodness of fit test, the test for independence does not compare a single observed variable to a theoretical population but rather two variables within a sample set to one another.

The hypotheses for a Chi-square test of independence are as follows:

Null Hypothesis (Ho) : There is no association between the two categorical variables in the population of interest.

Alternative Hypothesis (Ha) : There is no association between the two categorical variables in the population of interest.

The next step is to create a contingency table of expected values that reflects how a data set that perfectly aligns the null hypothesis would appear.

The simplest way to do this is to calculate the marginal frequencies of each row and column; the expected frequency of each cell is equal to the marginal frequency of the row and column that corresponds to a given cell in the observed contingency table divided by the total sample size.

Test for Homogeneity

The Chi-square test for homogeneity is organized and executed exactly the same as the test for independence.

The main difference to remember between the two is that the test for independence looks for an association between two categorical variables within the same population, while the test for homogeneity determines if the distribution of a variable is the same in each of several populations (thus allocating population itself as the second categorical variable).

Null Hypothesis (Ho) : There is no difference in the distribution of a categorical variable for several populations or treatments.

Alternative Hypothesis (Ha) : There is a difference in the distribution of a categorical variable for several populations or treatments.

The difference between these two tests can be a bit tricky to determine, especially in the practical applications of a Chi-square test. A reliable rule of thumb is to determine how the data was collected.

If the data consists of only one random sample with the observations classified according to two categorical variables, it is a test for independence. If the data consists of more than one independent random sample, it is a test for homogeneity.

What is the chi-square test?

The Chi-square test is a non-parametric statistical test used to determine if there’s a significant association between two or more categorical variables in a sample.

It works by comparing the observed frequencies in each category of a cross-tabulation with the frequencies expected under the null hypothesis, which assumes there is no relationship between the variables.

This test is often used in fields like biology, marketing, sociology, and psychology for hypothesis testing.

What does chi-square tell you?

The Chi-square test informs whether there is a significant association between two categorical variables. Suppose the calculated Chi-square value is above the critical value from the Chi-square distribution.

In that case, it suggests a significant relationship between the variables, rejecting the null hypothesis of no association.

How to calculate chi-square?

To calculate the Chi-square statistic, follow these steps:

1. Create a contingency table of observed frequencies for each category.

2. Calculate expected frequencies for each category under the null hypothesis.

3. Compute the Chi-square statistic using the formula: Χ² = Σ [ (O_i – E_i)² / E_i ], where O_i is the observed frequency and E_i is the expected frequency.

4. Compare the calculated statistic with the critical value from the Chi-square distribution to draw a conclusion.

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Gene variants foretell the biology of future breast cancers in Stanford Medicine study

In a finding that vastly expands the understanding of tumor evolution, researchers discover genetic biomarkers that can predict the breast cancer subtype a patient is likely to develop.

May 30, 2024 - By Krista Conger

Stanford Medicine researchers found that inherited gene sequences can predict what type of breast cancer a patient is likely to develop, along with how aggressive that cancer may be.   Emily Moskal

A Stanford Medicine study of thousands of breast cancers has found that the gene sequences we inherit at conception are powerful predictors of the breast cancer type we might develop decades later and how deadly it might be.

The study challenges the dogma that most cancers arise as the result of random mutations that accumulate during our lifetimes. Instead, it points to the active involvement of gene sequences we inherit from our parents — what’s known as your germline genome — in determining whether cells bearing potential cancer-causing mutations are recognized and eliminated by the immune system or skitter under the radar to become nascent cancers. 

“Apart from a few highly penetrant genes that confer significant cancer risk, the role of hereditary factors remains poorly understood, and most malignancies are assumed to result from random errors during cell division or bad luck,” said Christina Curtis , PhD, the RZ Cao Professor of Medicine and a professor of genetics and of biomedical data science. “This would imply that tumor initiation is random, but that is not what we observe. Rather, we find that the path to tumor development is constrained by hereditary factors and immunity. This new result unearths a new class of biomarkers to forecast tumor progression and an entirely new way of understanding breast cancer origins.”

Curtis is the senior author of the study, which will be published May 31 in Science . Postdoctoral scholar Kathleen Houlahan , PhD, is the lead author of the research.

“Back in 2015, we had posited that some tumors are ‘born to be bad’ — meaning that their malignant and even metastatic potential is determined early in the disease course,” Curtis said. “We and others have since corroborated this finding across multiple tumors, but these findings cast a whole new light on just how early this happens.”

A new take on cancer’s origin

The study, which gives a nuanced and powerful new understanding of the interplay between newly arisen cancer cells and the immune system, is likely to help researchers and clinicians better predict and combat breast tumors.

Currently, only a few high-profile cancer-associated mutations in genes are regularly used to predict cancers, but these account for a small minority of cases. Those include BRCA1 and BRCA2, which occur in about one of every 500 women and confer an increased risk of breast or ovarian cancer, and rarer mutations in a gene called TP53 that causes a disease called Li Fraumeni syndrome, which predisposes to childhood and adult-onset tumors.

Christina Curtis

The findings suggest there are tens or hundreds of additional gene variants — identifiable in healthy people — that through interactions with the immune system pull the strings that determine why some people remain cancer-free throughout their lives.

“Our findings not only explain which subtype of breast cancer an individual is likely to develop,” Houlahan said, “but they also hint at how aggressive and prone to metastasizing that subtype will be. Beyond that, we speculate that these inherited variants may influence a person’s risk of developing breast cancer. However, future studies will be needed to examine this.”  

The genes we inherit from our parents are known as our germline genome. They’re mirrors of our parents’ genetic makeup, and they can vary among people in small ways that give some of us blue eyes, brown hair or type O blood. Some inherited genes include mutations that confer increased cancer risk from the get-go, such as BRCA1, BRCA2 and TP53.

In contrast, most cancer-associated genes are part of what’s known as our somatic genome. As we live our lives, our cells divide and die in the tens of millions. Each time the DNA in a cell is copied, mistakes happen and mutations can accumulate. DNA in tumors is often compared with the germline genomes in blood or normal tissues in an individual to pinpoint which changes likely led to the cell’s cancerous transformation.

Classifying breast cancers

In 2012, Curtis began a deep dive — assisted by machine learning — into the types of somatic mutations that occur in thousands of breast cancers. She was eventually able to categorize the disease into 11 subtypes with varying prognoses and risk of recurrence, finding that four of the 11 groups were significantly more likely to recur even 10 or 20 years after diagnosis — critical information for clinicians making treatment decisions and discussing long-term prognoses with their patients.

Prior studies had shown that people with inherited BRCA1 mutations tend to develop a subtype of breast cancer known as triple negative breast cancer. This correlation implies some behind-the-scenes shenanigans by the germline genome that affects what subtype of breast cancer someone might develop.

“We wanted to understand how inherited DNA might sculpt how a tumor evolves,” Houlahan said. To do so, they took a close look at the immune system.

It’s a quirk of biology that even healthy cells routinely decorate their outer membranes with small chunks of the proteins they have bobbing in their cytoplasm — an outward display that reflects their inner style.

Kathleen Houlahan

The foundations for this display are what’s known as HLA proteins, and they are highly variable among individuals. Like fashion police, immune cells called T cells prowl the body looking for any suspicious or overly flashy bling (called epitopes) that might signal something is amiss inside the cell. A cell infected with a virus will display bits of viral proteins; a sick or cancerous cell will adorn itself with abnormal proteins. These faux pas trigger the T cells to destroy the offenders.

Houlahan and Curtis decided to focus on oncogenes, normal genes that, when mutated, can free a cell from regulatory pathways meant to keep it on the straight and narrow. Often, these mutations take the form of multiple copies of the normal gene, arranged nose to tail along the DNA — the result of a kind of genomic stutter called amplification. Amplifications in specific oncogenes drive different cancer pathways and were used to differentiate one breast cancer subtype from another in Curtis’ original studies.

The importance of bling

The researchers wondered whether highly recognizable epitopes would be more likely to attract T cells’ attention than other, more modest displays (think golf-ball-sized, dangly turquoise earrings versus a simple silver stud). If so, a cell that had inherited a flashy version of an oncogene might be less able to pull off its amplification without alerting the immune system than a cell with a more modest version of the same gene. (One pair of overly gaudy turquoise earrings can be excused; five pairs might cause a patrolling fashionista T cell to switch from tutting to terminating.)

The researchers studied nearly 6,000 breast tumors spanning various stages of disease to learn whether the subtype of each tumor correlated with the patients’ germline oncogene sequences. They found that people who had inherited an oncogene with a high germline epitope burden (read: lots of bling) — and an HLA type that can display that epitope prominently — were significantly less likely to develop breast cancer subtypes in which that oncogene is amplified.

There was a surprise, though. The researchers found that cancers with a large germline epitope burden that manage to escape the roving immune cells early in their development tended to be more aggressive and have a poorer prognosis than their more subdued peers.

“At the early, pre-invasive stage, a high germline epitope burden is protective against cancer,” Houlahan said. “But once it’s been forced to wrestle with the immune system and come up with mechanisms to overcome it, tumors with high germline epitope burden are more aggressive and prone to metastasis. The pattern flips during tumor progression.”

“Basically, there is a tug of war between tumor and immune cells,” Curtis said. “In the preinvasive setting, the nascent tumor may initially be more susceptible to immune surveillance and destruction. Indeed, many tumors are likely eliminated in this manner and go unnoticed. However, the immune system does not always win. Some tumor cells may not be eliminated and those that persist develop ways to evade immune recognition and destruction. Our findings shed light on this opaque process and may inform the optimal timing of therapeutic intervention, as well as how to make an immunologically cold tumor become hot, rendering it more sensitive to therapy.”

The researchers envision a future when the germline genome is used to further stratify the 11 breast cancer subtypes identified by Curtis to guide treatment decisions and improve prognoses and monitoring for recurrence. The study’s findings may also give additional clues in the hunt for personalized cancer immunotherapies and may enable clinicians to one day predict a healthy person’s risk of developing an invasive breast cancer from a simple blood sample.

“We started with a bold hypothesis,” Curtis said. “The field had not thought about tumor origins and evolution in this way. We’re examining other cancers through this new lens of hereditary and acquired factors and tumor-immune co-evolution.”

The study was funded by the National Institutes of Health (grants DP1-CA238296 and U54CA261719), the Canadian Institutes of Health Research and the Chan Zuckerberg Biohub.

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