phd thesis in tissue engineering

Tissue Engineering an In Vitro Model of Osteoporosis

--> Owen, Robert (2017) Tissue Engineering an In Vitro Model of Osteoporosis. PhD thesis, University of Sheffield.

Postmenopausal osteoporosis is a skeletal disorder characterised by bone loss. Declining oestrogen levels postmenopause disrupt bone remodelling by over-stimulating resorption. Although the disorder is currently studied in animals, we should aim to minimise their use. Therefore, this thesis explored the feasibility of developing an in vitro model of postmenopausal osteoporosis using tissue engineering principles. The response of three osteoblast cell lines, MC3T3-E1, MLOA5, and IDG-SW3, to oestrogen was explored, finding only MC3T3-E1 was stimulated by the hormone. The ability of RAW264.7 to undergo osteoclastogenesis was strongly influenced by seeding density and proliferation. Additionally, tartrate-resistant acid phosphatase (TRAP) activity could be suppressed by oestrogen exposure. Due to its ability to support osteoclastogenesis in co-culture, IDG-SW3 was the most suitable osteoblast cell line for the model. Bone-matrix deposition over 28 days on three scaffolds (PolyHIPE, polyurethane, Biotek) was compared to select the most appropriate for the model. PolyHIPE and polyurethane scaffolds supported significantly more matrix deposition than the Biotek. Mineralisation on the scaffold could be detected by micro-computed tomography; however, the presence of PBS interfered with this. Due to its cellular performance and ease of manufacture, the polyurethane scaffold was identified as the most suitable for the model. Changes in mineral content, TRAP and alkaline phosphatase activity were confirmed as markers for osteoclast and osteoblast activity in co-culture. RAW264.7 pre-treatment with oestrogen to mimic pre-menopause had lasting effects on their ability to undergo osteoclastogenesis. 2D co-cultures using oestrogen withdrawal to mimic menopause resulted in increased resorption, analogous to the effect seen in vivo. From the conditions assessed in 3D co-cultures, no equivalent response was observed. This thesis demonstrates it is possible to imitate the onset of postmenopausal osteoporosis in vitro. However, a 3D system that uses human cells and longer time periods is necessary to provide a valid alternative to animal models.

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Composite bone tissue engineering scaffolds produced by coaxial electrospinning

Kareem, Muna Mustafa (2018) Composite bone tissue engineering scaffolds produced by coaxial electrospinning. PhD thesis, University of Glasgow.

Electrospinning of polylactic acid (PLA)/calcium phosphates (CaPs) has been widely investigated for bone tissue engineering, however the significant reduction in mechanical properties and the rapid loss of the structural integrity of the scaffolds upon inclusion of high filler content is still challenging. Coaxial electrospinning has gained attention for tissue engineering applications due to the enhanced quality and the functionality of the resulting fibres compared to the basic electrospinning process. In this study, core and shell polycarpolactone (PCL)- PLA/micro-HA fibrous scaffolds were produced via coaxial electrospinning. To optimise the shell component, PLA solutions of concentrations ranging from 5 to 25 wt%, and containing 10-40 vol% of either spray dried HA (HA1), sintered HA (HA2) or beta tricalcium phosphate (β-TCP) were electrospun using single-needle electrospinning. However, only 15 and 20 wt% PLA solution with 10 or 20 vol% CaPs produced electrospun scaffolds. Inclusion of all these fillers significantly reduced the mechanical properties of the scaffolds compared to non-filled PLA while increasing fibre diameter and non-homogeneity. TCP-containing scaffolds showed reduced mechanical properties compared to HA1- and HA2-filled scaffolds and increased TCP agglomerations along the fibres. Introduction of HA1 and HA2 into PLA scaffolds decreased the degradation rate of the scaffolds while increasing the bioactivity. However, apatite formation on the fibre surfaces was lower than previously reported due to the lower surface area of micro-HA particles compared to nano-HA in addition to the lack of sufficient HA particles on fibres surface. The higher surface area of HA1 did not significantly affect the rate of bioactivity, however it increased the thermal stability of scaffolds compared to HA2-filled scaffolds and led to further reduction in mechanical properties in vitro than HA2. Scaffolds with either HA lost their mechanical integrity within 28 days of SBF immersion. As for the core component, changing the solvent system was found to affect the stability of the Taylor cone during electrospinning, and subsequently the morphology of the resultant fibre. Introduction of PCL as the core component in coaxial scaffolds increased both the tensile strength and strain at failure. The mechanical properties were influenced by the flow rate ratio between the core and shell components. Coaxial scaffolds with and without HA exhibited gradual release of BMP 2 with only 12.8-13.6% released over 96 hours. They also supported cell attachment and spreading over 21 days of culture. However, control scaffolds had improved cell spreading compared to HA-containing scaffolds due to increased fibre uniformity and decreased fibre diameter. Tubular scaffolds made of core and shell structured fibres were also produced using rotating needle collector with G16 and G21 needle producing the internal diameter. Coaxial electrospinning with rotating needle collector produced fibres with improved circumferential alignment compared to stationary collector and increased fibre non-uniformity in HA-containing scaffolds. Bioactivity of tubular coaxial scaffolds was also significantly increased due to partial encapsulation of HA particles, and large areas of coaxial scaffolds were covered with apatite layer after 12 weeks of immersion in SBF. On the other hand, coaxial scaffolds with no HA showed no bioactivity even after 12 weeks of SBF immersion. Tubular control and HAcontaining coaxial scaffolds had significantly higher mechanical stability in vitro and showed gradual reduction in their mechanical properties over 12 weeks of immersion in either PBS or SBF. The results obtained suggest that coaxial electrospinning is a promising technique to produce bone tissue scaffolds with high content of CaPs while preserving the structural and mechanical integrity of the scaffolds. Bioactivity of scaffolds can be significantly increased by incorporating the CaP in the shell layer while mechanical properties of the coaxial scaffolds can be tailored by changing the core composition and diameter. However, further studies should be carried on to enhance the uniformity and alignment of the coaxial fibres in order to improve the mechanical properties of scaffolds.

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Advances on Modeling in Tissue Engineering pp 137–176 Cite as

Biofabrication Strategies for Tissue Engineering

• Paulo Jorge Bártolo 3 ,
• Marco Domingos 3 ,
• Tatiana Patrício 3 ,
• Stefania Cometa 4 &
• Vladimir Mironov 5  
• First Online: 01 January 2011

1558 Accesses

15 Citations

Part of the book series: Computational Methods in Applied Sciences ((COMPUTMETHODS,volume 20))

The success of Tissue Engineering (TE) strongly relies on the capability of designing biomimetic scaffolds closely resembling the host tissue environment. Due to the functional multitude of the native tissues, the considerations are complex and include chemical, morphological, mechanical and biological factors and their mutability with time. Nonetheless, to trigger and/or assist the “natural healing mechanism’’ of the human body it seems essential to provide an appropriate biomechanical environment and biomolecular signalling to the cells. Novel biomanufacturing processes are increasingly being recognized as ideal techniques to produce 3D biodegradable structures with optimal pore size and spatial distribution, providing an adequate mechanical support for tissue regeneration while shaping in-growing tissues. In this chapter, we discuss in detail the most recent advances in the field of biofabrication, providing and updated overview of processes and materials employed in the production of tissue engineering constructs. Bioprinting or ‘’scaffold-less’’ strategies are also presented in this work. They are based on the precise deposition of high-density tissue spheroids or cell aggregates being advantageous alternatives to the current scaffold-based tissue engineering approach.

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Bártolo, P.J., Domingos, M., Patrício, T., Cometa, S., Mironov, V. (2011). Biofabrication Strategies for Tissue Engineering. In: Fernandes, P., Bártolo, P. (eds) Advances on Modeling in Tissue Engineering. Computational Methods in Applied Sciences, vol 20. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-1254-6_8

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Biomedical Engineering Theses and Dissertations

This collection contains theses and dissertations from the Department of Biomedical Engineering, collected from the Scholarship@Western Electronic Thesis and Dissertation Repository

Theses/Dissertations from 2024 2024

Co-delivery of Adipose-derived Stromal Cells and Endothelial Colony Forming Cells in Novel Cell-assembled Scaffolds as a Pro-angiogenic Cell Therapy Platform , Sarah A. From

Theses/Dissertations from 2023 2023

Multiparametric Classification of Tumor Treatment Using Ultrasound Microvascular Imaging , mahsa bataghva

Towards Patient Specific Mitral Valve Modelling via Dynamic 3D Transesophageal Echocardiography , Patrick Carnahan

Developing a Finite Element Model for Evaluating the Posterior Tibial Slope in a Medial Opening Wedge High Tibial Osteotomy , VIctor Alexander Carranza

Analysis and Characterization of Embroidered Textile Strain Sensors for Use in Wearable Mechatronic Devices , Jose Guillermo Colli Alfaro

Developing Bioactive Hydrogels Containing Cell-derived Extracellular Matrix for Bone and Cartilage Repair , Ali Coyle

Modelling of a TCA-driven Wearable Tremor Suppression Device for People with Parkinson’s Disease , Parisa Daemi

Using Machine Learning Models to Address Challenges in Lung Cancer Care , Salma Dammak

Longitudinal dynamics of cerebrospinal fluid Aꞵ, pTau and sTREM2 reveal predictive preclinical trajectories of Alzheimer’s pathology , Bahaaldin Helal

MAGNETIC RESONANCE IMAGING BIOMARKERS FOR PARKINSON’S DISEASE: A MACHINE LEARNING APPROACH , Dimuthu Henadeerage Don

Detecting Treatment Failure in Rheumatoid Arthritis with Time-Domain Diffuse Optical Methods , Seva Ioussoufovitch

Novel Magnetic Resonance Imaging-Compatible Mechatronic Needle Guidance System for Prostate Focal Laser Ablation Therapy , Eric R. Knull

The Development of Stimuli-responsive Hydrogels from Self-Immolative Polymers , Jared David Pardy

Free-hand Photoacoustic Imaging of Breast Cancer Tissue , Elina Rascevska

Development of a Cell-based Regenerative Strategy to Modulate Angiogenesis and Inflammation in Ischemic Muscle , Fiona E. Serack

Investigation of Dynamic Culture on Matrix-derived Microcarriers as a Strategy to Modulate the Pro-Regenerative Phenotype of Human Adipose-derived Stromal Cells , McKenna R. Tosh

Evaluating EEG–EMG Fusion-Based Classification as a Method for Improving Control of Wearable Robotic Devices for Upper-Limb Rehabilitation , Jacob G. Tryon

Theses/Dissertations from 2022 2022

A two-layer continuous-capillary oxygen transport model: Development and application to blood flow regulation in resting skeletal muscle. , Keith C. Afas

Development of a Hybrid Stereotactic Guidance System For Percutaneous Liver Tumour Ablation , Joeana N. Cambranis Romero

Large-scale Analysis and Automated Detection of Trunnion Corrosion on Hip Arthroplasty Devices , Anastasia M. Codirenzi

The Role of Transient Vibration of the Skull on Concussion , Rodrigo Dalvit Carvalho da Silva

Biomechanical Investigation of Complete and Partial Medial Collateral Ligament Injuries , Callahan Doughty

Towards A Comprehensive Software Suite for Stereotactic Neurosurgery , Greydon Gilmore

The Bio-Mechanical Development and Kinematic Evaluation of Zone I and Zone II Injuries and their Corresponding Surgical Repair Techniques using an In-Vitro Active Finger Motion Simulator: A Cadaveric Study , Mohammad Haddara

Image-based Cochlear Implant Frequency-to-Place Mapping , Luke William Helpard

Mechanical Evaluation of Gyroid Structures to Combat Orthopaedic Implant Infections , Sydney Hitchon

The Development of a Motion Sensing Device for Use in a Home Setting , Jaspreet K. Kalsi

A Novel Ultrasound Elastography Technique for Evaluating Tumor Response to Neoadjuvant Chemotherapy in Patients with Locally Advanced Breast Cancer , Niusha Kheirkhah

Thermo-responsive Antibiotic-Eluting Coatings for Treating Infection near Orthopedic Implants , Jan Chung Kwan

Effects of Modulating the Culture Microenvironment on the Growth and Secretome of Human Adipose-Derived Stromal Cells , Zhiyu Liang

Conducting Polypyrrole Hydrogel Biomaterials For Drug Delivery And Cartilage Tissue Regeneration , Iryna Liubchak

Motion and Crosslinked Polyethylene Wear in Reverse Total Shoulder Arthroplasty , Christopher Millward

Intracardiac Ultrasound Guided Systems for Transcatheter Cardiac Interventions , Hareem Nisar

Investigation of Cell Derived Nanoparticles for Drug Delivery and Osteogenic Differentiation of Human Stem/Stromal Cells , Shruthi Polla Ravi

Quantitative Image Analysis of White Matter Dysregulation Using Brain Normalization for Diagnostic Analysis of Pediatric Hydrocephalus , Renee-Marie Ragguett

Automation through Deep-Learning to Quantify Ventilation Defects in Lungs from High-Resolution Isotropic Hyperpolarized 129Xe Magnetic Resonance Imaging , Tuneesh Kaur Ranota

Early Biological Response of Articular Cartilage to Hemiarthroplasty Wear , Debora Rossetti

Sol-Gel Derived Bioceramic Poly(Diethyl Fumarate – Co – Triethoxyvinylsilane) Composite , Aref Sleiman

The Application of Digital Volume Correlation Bone Strain Measurements in the Osteoarthritic Glenohumeral Joint , Jakub R. Targosinski

Development of Brain-Derived Bioscaffolds for Neural Progenitor Cell Culture and Delivery , Julia Terek

Modelling and Evaluation of Piezoelectric Actuators for Wearable Neck Rehabilitation Devices , Shaemus D. Tracey

Development of a Combined Experimental-Computational Framework to Study Human Knee Biomechanics , Samira Vakili

Investigation on the Performance of Dry Powder Inhalation System for Enhanced Delivery of Levosalbutamol Sulfate , Yuqing Ye

Theses/Dissertations from 2021 2021

Development of a Wireless Telemetry Load and Displacement Sensor for Orthopaedic Applications , William Anderson

Organic-Inorganic Hybrid Biomaterials for Bone Tissue Engineering and Drug Delivery , Neda Aslankoohi

Fabrication Of Inkjet-Printed Enzyme-Based Biosensors Towards Point-Of-Care Applications , Yang Bai

The Use of CT to Assess Shoulder Kinematics and Measure Glenohumeral Arthrokinematics , Baraa Daher

The Development of Region-Specific Decellularized Meniscus Bioinks for 3D Bioprinting Applications , Sheradan Doherty

In Vitro Analyses of the Contributions of the Hip Capsule to Joint Biomechanics , Emma Donnelly

Long-Circulating, Degradable Lanthanide-Based Contrast Agents for Pre-Clinical Microcomputed Tomography of the Vasculature , Eric Grolman

Mixed-reality visualization environments to facilitate ultrasound-guided vascular access , Leah Groves

Diffusion Kurtosis Imaging in Temporal Lobe Epilepsy , Loxlan W. Kasa

Extracellular Matrix-Derived Microcarriers as 3-D Cell Culture Platforms , Anna Kornmuller

3D Printed Polypyrrole Scaffolds for pH Dependent Drug Delivery with Applications in Bone Regeneration , Matthew T. Lawrence

Development of Multifunctional Drug Delivery Systems for Locoregional Therapy , Xinyi Li

Motion Intention Estimation using sEMG-ACC Sensor Fusion , Jose Alejandro Lopez

Biomaterial for Cervical Intervertebral Disc Prosthesis , Helium Mak

Biomechanical Analysis of Ligament Modelling Techniques and Femoral Component Malrotation Following TKA , Liam A. Montgomery

Snapshot Three-Dimensional Surface Imaging With Multispectral Fringe Projection Profilometry , Parsa Omidi

4DCT to Examine Carpal Motion , Sydney M. Robinson

Seizure Detection Using Deep Learning, Information Theoretic Measures and Factor Graphs , Bahareh Salafian

Modeling Fetal Brain Development: A semi-automated platform for localization, reconstruction, and segmentation of the fetal brain on MRI , Jianan Wang

Immobilized Jagged1 for Notch3-specific Differentiation and Phenotype Control of Vascular Smooth Muscle Cells , Kathleen E. Zohorsky

Theses/Dissertations from 2020 2020

Simulation Approaches to X-ray C-Arm-based Interventions , Daniel R. Allen

Implementing a multi-segment foot model in a clinical setting to measure inter-segmental joint motions , Tahereh Amiri

Cardiac Modelling Techniques to Predict Future Heart Function and New Biomarkers in Acute Myocardial Infarction , Sergio C. H. Dempsey

Feasibility of Twisted Coiled Polymer Actuators for Use in Upper Limb Wearable Rehabilitation Devices , Brandon P.R. Edmonds

Metal Additive Manufacturing for Fixed Dental Prostheses , Mai EL Najjar

Using an Internal Auditory Stimulus to Activate the Developing Primary Auditory Cortex: A Fetal fMRI Study , Estee Goldberg

Development of Water-Soluble Polyesters for Tissue Engineering Applications , Trent Gordon

Development Of Hybrid Coating Materials To Improve The Success Of Titanium Implants , Zach Gouveia

A 3D Printed Axon-Mimetic Diffusion MRI Phantom , Tristan K. Kuehn

Development of an Active Infection Monitoring Knee Spacer for Two-Stage Revision , Michael K. Lavdas

Computational Modeling of the Human Brain for mTBI Prediction and Diagnosis , Yanir Levy

Pulmonary Imaging of Chronic Obstructive Pulmonary Disease using Multi-Parametric Response Maps , Jonathan MacNeil

Optimization of Indentation for the Material Characterization of Soft PVA-Cryogels , Md. Mansur ul Mulk

Development and Validation of Augmented Reality Training Simulator for Ultrasound Guided Percutaneous Renal Access , Yanyu Mu

A Biomechanical Investigation into the Effect of Experimental Design on Wrist Biomechanics and Contact Mechanics , Clare E. Padmore

Structure-Function Relationships in the Brain: Applications in Neurosurgery , Daiana-Roxana Pur

The Effect of Joint Alignment After a Wrist Injury on Joint Mechanics and Osteoarthritis Development , Puneet Kaur Ranota

Development and Validation of Tools for Improving Intraoperative Implant Assessment with Ultrasound during Gynaecological Brachytherapy , Jessica Robin Rodgers

Studies on Carbon Quantum Dots with Special Luminescent Properties and Their Capability of Overcoming the Biological Barriers , Ji Su Song

Machine Learning towards General Medical Image Segmentation , Clara Tam

The Migration and Wear of Reverse Total Shoulder Arthroplasty , Madeleine L. Van de Kleut

Video Processing for the Evaluation of Vascular Dynamics in Neurovascular Interventions , Reid Vassallo

Preparation of Intra-articular Drug Delivery Systems for the Treatment of Osteoarthritis , Ian Villamagna

Deep Reinforcement Learning in Medical Object Detection and Segmentation , Dong Zhang

Theses/Dissertations from 2019 2019

Fabrication and Characterization of Collagen-Polypyrrole Constructs Using Direct-Ink Write Additive Manufacturing , Rooshan Arshad

Development of a Force-Based Ream Vector Measurement System For Glenoid Reaming Simulation , David Axford

Investigation of Visual Perceptions in Parkinson's Disease and the Development of Disease Monitoring Software , Matthew Bernardinis

Tissue Equivalent Gellan Gum Gel Materials for Clinical MRI and Radiation Dosimetry , Pawel Brzozowski

Implementation of User-Independent Hand Gesture Recognition Classification Models Using IMU and EMG-based Sensor Fusion Techniques , José Guillermo Collí Alfaro

Scaffold Design Considerations for Soft Tissue Regeneration , Madeleine M. Di Gregorio

Remote Navigation and Contact-Force Control of Radiofrequency Ablation Catheters , Daniel Gelman

High-throughput Fabrication of Drug-loaded Core-shell Tablets with Adjustable Release Profiles from Surface-erodible and Photocrosslinkable Polyanhydrides , Armin Geraili Nejadfomeshi

Apply dry powder on drug loading and enteric coating of esomeprazole magnesium trihydrate beads and capsules , Xiaojing Ge

Bioluminescence resonance energy transfer (BRET) - based nanostructured biosensor for detection of glucose , Eugene Hwang

A Heterogeneous Patient-Specific Biomechanical Model of the Lung for Tumor Motion Compensation and Effective Lung Radiation Therapy Planning , Parya Jafari

The Co-Delivery of Syngeneic Adipose-Derived Stromal Cells and Macrophages on Decellularized Adipose Tissue Bioscaffolds for In Vivo Soft Tissue Regeneration , Hisham A. Kamoun

Improving Material Mapping in Glenohumeral Finite Element Models: A Multi-Level Evaluation , Nikolas K. Knowles

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PhD Defence Human Liver Tissue Engineering - From Organoids to Tissues

End-stage liver diseases cause more than two million deaths yearly worldwide. Currently, the only effective treatment for end-stage liver diseases is liver transplantation. While less than 10% of patients obtain a suitable donor liver for transplantation, many patients are dying on the waiting list. Therefore, there is an urgent need to find alternatives for donor livers for transplantation.

In the PhD dissertation “Human Liver Tissue Engineering- From Organoids to Tissues”, Shicheng Ye et al. describes his achievements towards creating mini liver tissues that can be explored for transplantation purposes in the future. Part I of the thesis focuses on developing animal-free and well-defined hydrogels for liver organoids, a source for the main epithelial liver cell types. These hydrogels are promising to expand and differentiate the liver organoids into functional liver cells without the use of animal derived materials. In Part II, Shicheng established two bioreactor-based methods for large-scale and rapid production of liver organoids. These two methods bridge the gap between tedious static organoid culture in hydrogel droplets and the need for a large number of organoids for liver tissue engineering. In Part III, Shicheng developed a strategy to create mini liver tissues with multiple different liver cell types in well-defined hydrogels and under dynamic fluidic stimuli.

To conclude, the achievements made in this thesis can be applied as advanced in vitro models for fundamental studies, including liver development and disease modelling and can be seen as the foundation for translational research and possible clinical applications in the future.

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The Serpooshan Team uses a multidisciplinary approach to design and develop micro/nano-scale tissue engineering technologies with the ultimate goal of generating functional tissues and organs. Current projects in my lab include:

1) Bioengineering iPSC-based, functional cardiovascular tissue models, via 3D bioprinting technologies, as in vitro platforms to study human heart development and diseases;

2) Design and manufacturing novel patient-specific cardiac patch systems to regenerate damaged myocardium following ischemic heart injury;

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4) Engineering highly tunable bioprinted models to study pediatric tumor growth, aggression, and response to therapies.  

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Modeling human heart development & congenital heart disease

Using state-of-the-art 3D bioprinting, perfusion bioreactor, and induced pluripotent stem cell technologies to engineer in vitro models of human heart development and diseases. Studying various cellular and environmental factors underlying congenital heart defects and their therapies.

Advanced cardiac patch devices to regenerate damaged heart tissue

Using a variety of bioengineering approaches, together with human induced pluripotent stem cell technologies and paracrine signalling, to create novel biomaterial-based cardiac patch devices. These patient-specific scaffolds are capable of regenerating the damaged heart tissue following acute injuries such as heart attack.

Smart Nanobiomaterials

Synthesis, characterization, and application of various nano/micro-scale biomaterials for diverse biomedical applications in vitro and in vivo. Examples include: magnetic nanoparticles for antibacterial and imaging applications, conductive nanorods to enhance cardiac tissue function, and nanostructured scaffolds.

Meet The Team

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Vahid Serpooshan, PhD

Principal investigator.

Vahid Serpooshan did his BSc and MSc in Materials Science and Engineering at Sharif University (Tehran, Iran, 1998-2003) and his PhD in biomaterials and tissue engineering at McGill University (Montreal, Canada, 2007-2011). His PhD thesis research focused on the design and optimization of scaffolding biomaterials for bone tissue engineering applications. Following his PhD, Dr. Serpooshan worked for 7 years at Stanford University School of Medicine as Postdoctoral Fellow (Pediatric Cardiology) and Instructor (Stanford Cardiovascular Institute).  At Stanford, Dr. Serpooshan's training and research were mainly centered on developing a new generation of engineered cardiac patch device to repair damaged heart tissue following myocardial infarction (heart attack). The engineered patch was successfully tested in mouse and pig models and is now in preparation for clinical trials. He also worked on enabling technologies for human-machine hybrid cardiac tissue, using 3D bioprinting to assemble complex arrays of interfaces between synthetic and biological materials. In 2018, Dr. Vahid Serpooshan joined Emory University and Georgia Institute of Tech as Assistant Professor of Biomedical Engineering and Pediatrics, where his multidisciplinary team is now working on a variety of 3D bioprinting-based tissue engineering and disease modeling projects.

Assistant Professor of Biomedical Engineering

and Pediatrics

Georgia Institute of Technology

Emory University School of Medicine

     ​

404.712.9717

[email protected]

Holly Bauser-Heaton is an interventional pediatric cardiologist and physician scientist at Sibley Heart Center at Children’s Healthcare of Altanta.  She completed her MD, PhD in 2009 at Indiana University.  Dr. Bauser-Heaton focused on signaling mechanisms of nitric oxide in hypoxic conditions and continues to investigate the role of NO in endothelial function.  As a clinician, she completed her training at Stanford University and joined faculty of Sibley Heart Center in 2016.  Pulmonary artery disease and its management is the focus for Dr. Heaton both in the clinical arena and the lab.  She is interested in developing new procedures via transcatheter (in the cardiac catheterization laboratory) technique for individuals with pulmonary artery disease.  Additionally, she has interest in utilizing 3D bioprinting to create pulmonary artery constructs that have the ability to keep up with a patient’s somatic growth. She is very excited to join forces with the Serpooshan lab.

Holly Bauser-Heaton, MD PhD

Assistant professor, pediatric cardiologist.

Sibley Heart Center, Pediatric Cardiology

​ Emory University School of Medicine

​   

[email protected]

Sarah Rezapour, MD

Sarah Rezapour earned her MD degree from Tehran University of Medical Sciences (Tehran, Iran) in 2015. Following the completion of her degree, she embarked on a three-year practice in the field of medicine, gaining invaluable experience in patient care and medical practice. With a passion for delving deeper into the unmet needs in clinical settings, Dr. Rezapour transitioned to the research arena, joining the Tehran Research Institute of Gastrointestinal and Liver Diseases. Her project was directed toward unraveling the complex pathways involved in non-alcoholic steatohepatitis (NASH) progression and developing novel approaches for diagnosis and treatment. Sarah joined the Serpooshan lab in May 2023 as a postdoctoral research fellow. In her current role, she mainly focuses on Fontan-associated liver disease (FALD). Her project centers on biofabrication and in vitro culture strategies for constructing a vascular 3D model of the healthy human liver (hepatic sinusoid) vs. FALD tissue models.

Postdoctoral Fellow

Departments of Biomedical Engineering and Pediatrics

[email protected]

Mehdi Salar Amoli received his BSc from Amirkabir University of Technology (Tehran Polytechnique, Iran), studying biomaterials and tissue engineering, and performing research on osteochondral tissue engineering. Next, he moved to London, where he continued studies in the field of biomaterials and tissue engineering at Imperial College London (ICL), conducting research at Dr. Molly Stevens group on nanoneedle based gene delivery techniques. Following his training at ICL, Mehdi moved to Belgium, where he received his PhD from KU Leuven. His doctoral research focused on development of bioinks for 3D bioprinting strategies aimed at regeneration of the dentoalveolar tissues. During his PhD, he focused on development of a range of stimuli-responsive biomaterials for bioprinting, sustained release delivery systems for incorporation of drugs and growth factors, as well as physical, mechanical and biological characterization of tissue engineered constructs. In 2023, Mehdi joined Serpooshan lab, where his main research focus is to develop in vitro disease models using bioprinting.

Mehdi Salar Amoli, PhD

[email protected]

Jemma Hwang, BS, BA

Lead research specialist.

[email protected]

Jemma Hwang received her BS in Bioengineering and BA in German Studies (2019) from the University of Georgia where they began their research endeavors in epigenetics to identify mutants in N. crassa that are defective in facultative heterochromatin.  In 2017, they obtained an internship at the Perfusion Systems Lab of Merck KGaA in Darmstadt, Germany to improve existing downstream processes of protein production in industry settings. In the Summer of 2018, they participated in the Summer Undergraduate Research Program (SURP) with the Sackler Institute at New York University, studying the electrophysiology and molecular biology of ATP-sensitive potassium channels. After completing their Bachelor’s, they accepted a position at the Duke Human Vaccine Institute to contribute to the ongoing development of vaccines against HIV-1. In December 2022, Jemma joined the Serpooshan-Bauser-Heaton Labs as the Lead Research Specialist. They are using their management experience to support active projects within the lab, while also leading the synthesis of gelatin methacrylate (GelMA).

Yamini Singh, B.Tech, Sc.M.

Research specialist.

[email protected]

Yamini Singh received her B.Tech. in Biomedical Engineering from Manipal Institute of Technology, India (2021) and her Sc.M. in Biomedical Engineering from Brown University (2023). While at Brown, she worked in the laboratories of Dr. Kareen Coulombe and Dr. Anita Shukla for her Master’s thesis which was focused on the optimization of hydrogels for core-shell 3D bioprinting of patterned vessels for engineered cardiac tissues. Yamini joined the Serpooshan Lab in August 2023 as a Research Specialist, contributing both to the lab managerial workflow as well as working on several research projects on the 3D bioprinted in vitro models of diseases and therapies.

Graduate Students

Carmen J. Gil

Phd student.

Wallace H. Coulter Department of Biomedical Engineering

Emory University and

Georgia Institute of Technology

[email protected]

Wallace H. Coulter Department of Biomedical Engineering

[email protected]

Lama Rita El Shammas

[email protected]

Boeun Hwang

[email protected]

Zahra Alghoul

[email protected]

Manasvi Parab

[email protected]

Melissa Cadena

[email protected]

Maher Saadeh

[email protected]

Vani Sridhar

[email protected]

Undergraduate Students

Lauren Korsnick

Undergraduate researcher.

Amritaa Basu

Wyatt DeBord

Sarah Fineman

Roshni Nandwani

Emory University

Pranitha Kaza

Sophia Norton

Margaret wade

Martin L. Tomov, PhD

Now: Associate Director, Innovation Cores @ Emory School of Medicine

Liqun Ning, PhD

Now: Assistant Professor @ Cleveland State University

Zinah Ghazi

N ow: Keller Williams Realty

Stefano Zanella

Andrea theus, mostafa abdalla, bme ms student.

Now: Medtronic

Now: Assoc Engineer at BD

Katherine Pham Do

N ow: Medical School

Jayce Schwartz

Rosemary Tran

Caroline Harpole

Alex Cetnar

Gabriella kabboul, joy e. nish.

Now: R&D Eng II @ Medtronic

BME MS Program

Now: BME PhD Student @ Serpooshan Lab

Now: PhD Student @ Levit Lab

Davis White

Corinna shimalla, victor putaturo.

​ Emory University

​ Georgia Institute of Technology

Federico Prokopczuk

Hannah komanapalli, ​ sure program 2018.

California State University, Northridge

SURE Program 2018

Purdue University

Kevin M. McCoy

Rommi Kashlan

Rebecca R. Xu

High school intern.

Now @: BME Undergrad at Georgia Tech

Bryanna J. Lima

Rithvik Sarasani

Alpravinta Alagesan

Agastya vaidya, allison wombwell.

RECENT PUBLICATIONS

L. Ning, J. Shim, M.L. Tomov, R. Liu, R. Mehta, A. Mingee, B. Hwang, L. Jin, A. Mantalaris, C. Xu, M. Mahmoudi, K.C. Goldsmith, V. Serpooshan. A 3D bioprinted in vitro model of neuroblastoma recapitulates dynamic tumor-endothelial cell interactions contributing to solid tumor aggressive behavior. Advanced Science , 9 (23), 2200244, 2022 .

J.W. Buikema, …, V. Serpooshan, S. Jovinge, B.K. Fleischmann, P.A. Doevendans, J. van der Velden, K.C. Garcia, J.C. Wu, J. PG Sluijter, and S.M. Wu. Wnt activation and reduced cell-cell contact synergistically induce massive expansion of functional human iPSC-derived cardiomyocytes. Cell Stem Cell , 27, 50-63, 2020 .

M. Mahmoudi, M. Yu, V. Serpooshan, J.C. Wu, R. Langer, R.T. Lee, J.M. Karp, and O.C. Farokhzad. Multiscale technologies for treatment of ischemic cardiomyopathy. Nature Nanotechnology , 12, 845-55, 2017 .

K. Wei+, V. Serpooshan+ (Co-First Author), …, M.D. Schneider, M.J.B. van den Hoff, M.J. Butte, P.C. Yang, K. Walsh, B. Zhou, D. Bernstein, M. Mercola, and P. Ruiz-Lozano. Epicardial FSTL1 reconstitution regenerates the adult mammalian heart. Nature , 525, 479-85, 2015 .

S. Chen, M.L. Tomov, L. Ning, C.J. Gil, B. Hwang, H.D. Bauser‐Heaton, H. Chen, and V. Serpooshan. Extrusion‐based 3D bioprinting of adhesive tissue engineering scaffolds using hybrid functionalized hydrogel bioinks. Advanced Biology , 7 (7), 2300124, 2023 .

S. Neelakantan, M. Kumar, E.A. Mendiola, H. Phelan, V. Serpooshan, S. Sadayappan, R. Avazmohammadi. Multiscale characterization of left ventricle active behavior in the mouse. Acta Biomaterialia , 162, 240-253, 2023 .

M.J. Hajipour, R. Safavi‐Sohi, S. Sharifi, N. Mahmoud, A.A. Ashkarran, E. Voke, V. Serpooshan, M. Ramezankhani, A.S. Milani, M.P. Landry, and M. Mahmoudi. An overview of nanoparticle protein corona literature. Small , 19 (36), 2301838, 2023 .

L. Ning and V. Serpooshan. Giant salamander-derived hydrogel granules with superior bioadhesive properties. Matter , 5 (9), 2581-2584, 2022 .

A.S. The us, L. Ning, G. Kabboul, B. Hwang, M.L. Tomov, C.N. LaRock, H.D. Bauser-Heaton, M. Mahmoudi, V. Serpooshan. 3D bioprinting of nanoparticle-laden hydrogel scaffolds with enhanced antibacterial and imaging properties. iScience , 25 (9), 104947, 2022 .

L. Ning, M.L. Tomov, S. Zanella, B. Zambrano, R. Avazmohammadi, M. Mahmoudi, H.D. Bauser-Heaton, and V. Serpooshan. Magnetic nanoparticle-mediated targeting of endothelium to address restenosis in a bioprinted in vitro model of pulmonary arteries. Arteriosclerosis, Thrombosis, and Vascular Biology , 42, A550, 2022 .

S. Sharifi, A. Ata Saei, H. Gharibi, N.N. Mahmoud, S. Harkins, N. Dararatana, E.M. Lisabeth, V. Serpooshan, Á. Végvári, A. Moore, M. Mahmoudi. Mass spectrometry, structural analysis, and anti-inflammatory properties of photo-cross-linked human albumin hydrogels. ACS Applied Bio Materials , 5 (6), 2643-2663, 2022 .

J.Q. Ho, M.R. Sepand, B. Bigdelou, T. Shekarian, R. Esfandyarpour, P. Chauhan, V. Serpooshan, L.K. Beura, G. Hutter, S. Zanganeh. The immune response to COVID‐19: Does sex matter? Immunology , 166 (4), 429-443, 2022 .

C.J. Gil, L. Li, B. Hwang, M. Cadena, A.S. Theus, T.A. Finamore, H. Bauser-Heaton, M. Mahmoudi, R.K. Roeder, V. Serpooshan. Tissue engineered drug delivery vehicles: Methods to monitor and regulate the release behavior. Journal of Controlled Release , 349, 143-155, 2022 .

L. Li, C.J. Gil, T.A. Finamore, C.J. Evans, M.L. Tomov, L. Ning, A.S. Theus, G. Kabboul, V. Serpooshan, R.K. Roeder. Methacrylate-modified gold nanoparticles enable noninvasive monitoring of photocrosslinked hydrogel scaffolds. Advanced NanoBiomed Research , 2 (7), 2200022, 2022 .

M.L. Tomov, L. Perez, L. Ning, H. Chen, ..., J. Zhang, R. Avazmohammadi, A. Mantalaris, B.D. Lindsey, D. Frakes, L.P. Dasi, V. Serpooshan, and H. Bauser-Heaton. A 3D Bioprinted In Vitro Model of Pulmonary Artery Atresia to Evaluate Endothelial Cell Response to Microenvironment. Advanced Healthcare Materials , 10 (20), 2100968, 2021 .

M. Mahmoudi, V. Serpooshan, P.C. Yang, and M. Heydarpour. The effect of cell sex on magnetic nanoparticle uptake of human induced pluripotent stem cell-derived cardiomyocytes. Circulation Research , 129, AP392-AP392, 2021 .

C.J. Gil, C. Evans, L. Li, M. Vargas, G. Kabboul, T. Fulton, R. Veneziano, N. Willet, H. Bauser-Heaton, R.K. Roeder, and V. Serpooshan. A precision medicine approach for non-invasive, longitudinal, and quantitative monitoring of cardiac tissue-engineered scaffolds. Circulation Research , 129, AMP207-AMP207, 2021 .

M. Mahmoudi, P.C. Yang, , P. Abadi, and M. Heydarpour. The effect of cell sex on cardiogenic differentiation of human induced pluripotent stem cells and their maturation processes. Circulation Research , 129, A109-A109, 2021 .

S. Chen, M.L. Tomov, L. Ning, C.J. Gil, B. Hwang, H. Bauser-Heaton, and V. Serpooshan. Three-dimensional bioprinting of adhesive cardiac patch systems. Circulation Research , 129, AP403-AP403, 2021 .

M. Zhao, Y. Nakada, Y. Wei, W. Bian, Y. Chu, A.V. Borovjagin, M. Xie, W. Zhu, T. Nguyen, Y. Zhou, V. Serpooshan, G.P. Walcott, and J. Zhang. Cyclin D2 Overexpression Enhances the Efficacy of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Myocardial Repair in a Swine Model of Myocardial Infarction. Circulation , 144 (3), 210-228, 2021 .

M.J. Hajipour, H. Aghaverdi, V. Serpooshan, H. Vali, S. Sheibani, and M. Mahmoudi. Sex as an important factor in nanomedicine. Nature Communications , 12, 2984-3010, 2021 .

L. Ning, N. Zhu, A. Smith, A. Rajaram, H. Hou, S. Srinivasan, F. Mohabatpour, L. He, A. Mclnnes, V. Serpooshan, P. Papagerakis, and X. Chen. Noninvasive Three-Dimensional In Situ and In Vivo Characterization of Bioprinted Hydrogel Scaffolds Using the X-ray Propagation-Based Imaging Technique. ACS Applied Materials & Interfaces , 13, 25611, 2021 .

O. Chirikian, W.R. Goodyer, E. Dzilic, V. Serpooshan, J.W. Buikema, W. McKeithan, H. Wu, G. Li, S. Lee, M. Merk, F. Galdos, A. Beck, A. JS Ribeiro, S. Paige, M. Mercola, J.C. Wu, B.L. Pruitt, and S.M. Wu. CRISPR/Cas9-based targeting of fluorescent reporters to human iPSCs to isolate atrial and ventricular-specific cardiomyocytes. Scientific Reports , 11, 1, 2021 .

M. Shokouhimehr, A.S. Theus, A. Kamalakar, L. Ning, C. Cao, M.L. Tomov, J.M. Kaiser, S. Goudy, N.J. Willett, H.W. Jang, C.N. LaRock, P. Hanna, A. Lechtig, M. Yousef, J. Da Silva Martins, A. Nazarian, M.B. Harris, M. Mahmoudi, V. Serpooshan. 3D Bioprinted Bacteriostatic Hyperelastic Bone Scaffold for Damage-Specific Bone Regeneration. Polymers , 13, 1099, 2021 (Editor's Choice).

Emory (primary) lab address:

1750 Haygood Dr.

HSRB-II Building

Room S435 (4th floor)

Atlanta, GA  30322

---------------------------

Georgia Tech address:

313 Ferst Dr NW

Georgia Inst. of Tech.

Atlanta, GA 30332

[email protected]

Office: 404-712-9717

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Home > College of Engineering > Dept. of Biomedical Engineering > Dissertations, Master’s Theses and Master’s Reports

Dept. of Biomedical Engineering Dissertations, Master’s Theses and Master’s Reports

Explore our collection of dissertations, master's theses and master's reports from the Department of Biomedical Engineering below.

Theses/Dissertations/Reports from 2023 2023

Collagen V Promotes Fibroblast Contractility, And Adhesion Formation, And Stability , Shaina P. Royer-Weeden

Theses/Dissertations/Reports from 2022 2022

AN ANTIMICROBIAL POLYDOPAMINE SURFACE COATING TO REDUCE BIOFOULING ON TELEMETRY TAGS USED IN MARINE CONSERVATION PRACTICES , Ariana Smies

ELECTROCHEMICAL APPROACHES TO CONTROL CATECHOL-BASED ADHESION , Md Saleh Akram Bhuiyan

Theses/Dissertations/Reports from 2021 2021

CHARACTERIZATION OF PROLIFERATION AND MIGRATION OF BREAST CANCER CELLS TARGETED BY A GLUT5-SPECIFIC FRUCTOSE MIMIC , Srinivas Kannan

IMPACT OF HEMODYNAMIC VORTEX SPATIAL AND TEMPORAL CHARACTERISTICS ON ANALYSIS OF INTRACRANIAL ANEURYSMS , Kevin W. Sunderland

Investigation into the Hemodynamics of Aortic Abnormalities Through Computational Fluid Dynamics , Tonie Johnson

MODEL POLYMER SYSTEMS CONTAINING CATECHOL MOIETIES TO TUNE HYDROGEN PEROXIDE GENERATION FOR ANTIPATHOGENIC AND WOUND HEALING APPLICATIONS , Pegah Kord Fooroshani

Theses/Dissertations/Reports from 2020 2020

ARTIFICIAL SYNTHETIC SCAFFOLDS FOR TISSUE ENGINEERING APPLICATION EMPHASIZING THE ROLE OF BIOPHYSICAL CUES , Samerender Nagam Hanumantharao

DEVELOPMENT AND VALIDATION OF THE FLOW CHAMBER FOR SHEAR FLOW MECHANOTRANSMISSION STUDIES , Mohanish Chandurkar

ELECTROSPUN NANOFIBER SCAFFOLDS AS A PLATFORM FOR BREAST CANCER RESEARCH , Carolynn Que

Nanofiber Scaffolds as 3D Culture Platforms , Stephanie Bule

STUDY OF SILICA NANOPARTICLE COMPOSITE ON SILICA-HYDROGEN PEROXIDE COMPLEXATIONS AND THEIR EFFECTS IN CATECHOL BASED ADHESIVES , Rattapol Pinnaratip

Theses/Dissertations/Reports from 2019 2019

AN INVESTIGATION OF UNCERTAINTY IN ULTRASONIC ELASTOGRAPHY: A CONTINUUM BIOMECHANICS PERSPECTIVE , David P. Rosen

A Smart Implantable Bone Fixation Plate Providing Actuation and Load Monitoring for Orthopedic Fracture Healing , Brad Nelson

DEGRADABLE ZINC MATERIAL CHARACTERISTICS AND ITS INFLUENCE ON BIOCOMPATIBILITY IN AN IN-VIVO MURINE MODEL , Roger J. Guillory II

MAGNETOSTRICTIVE BONE FIXATION DEVICE FOR CONTROLLING LOCAL MECHANICAL STIMULI TO BONE FRACTURE SITES , Salil Sidharthan Karipott

OPTICAL VORTEX AND POINCARÉ ANALYSIS FOR BIOPHYSICAL DYNAMICS , Anindya Majumdar

TOWARD AN UNDERSTANDING OF THE CLINICAL RELEVANCE OF NITRIC OXIDE (NO) MEASUREMENTS IN IN VITRO CELL CULTURE STUDIES , Maria Paula Kwesiga

Theses/Dissertations/Reports from 2018 2018

AN INJECTABLE THERMOSENSITIVE BIODEGRADABLE HYDROGEL EMBEDDED WITH SNAP CONTAINING PLLA MICROPARTICLES FOR SUSTAINED NITRIC OXIDE (NO) DELIVERY FOR WOUND HEALING , Nikhil Mittal

EFFECTS OF TOPOGRAPHICAL FEATURES ON MICROVASCULAR NETWORK FORMATION , Dhavan D. Sharma

REVERSIBLY SWITCHING ADHESION OF SMART ADHESIVES INSPIRED BY MUSSEL ADHESIVE CHEMISTRY , Ameya R. Narkar

Studying mass and mechanical property changes during the degradation of a bioadhesive with mass tracking, rheology and magnetoelastic (ME) sensors , Zhongtian Zhang

Theses/Dissertations/Reports from 2017 2017

A 3D Biomimetic Scaffold using Electrospinning for Tissue Engineering Applications , Samerender Nagam Hanumantharao

A WIRELESS, PASSIVE SENSOR FOR MEASURING TEMPERATURE AT ORTHOPEDIC IMPLANT SITES FOR EARLY DIAGNOSIS OF INFECTIONS , Salil Sidharthan Karipott

COMPUTATIONAL ULTRASOUND ELASTOGRAPHY: A FEASIBILITY STUDY , Yu Wang

DESIGN OF ROBUST HYDROGEL BASED ON MUSSEL-INSPIRED CHEMISTRY , Yuan Liu

EFFECT OF SILICA MICRO/NANO PARTICLES INCORPORATION OVER BIOINSPIRED POLY (ETHYLENE GLYCOL)-BASED ADHESIVE HYDROGEL , Rattapol Pinnaratip

FABRICATION OF PREVASCULARIZED CELL-DERIVED EXTRACELLULAR MATRIX BASED BIOMIMETIC TISSUE CONSTRUCTS FOR MULTIPLE TISSUE ENGINEERING , Zichen Qian

IDENTIFICATION OF NITRIC-OXIDE DEGRADATION PRODUCTS OF ASCORBIC ACID , Sushant Satyanarayan Kolipaka

Implantable Wireless Sensor Networks: Application to Measuring Temperature for In Vivo Detection of Infections , Praharsh Madappaly Veetil

SYSTEMATIC STUDY OF HYDROGEN PEROXIDE GENERATION, BIOCOMPATIBILITY AND ANTIMICROBIAL PROPERTY OF MUSSEL ADHESIVE MOIETY , Hao Meng

Theses/Dissertations/Reports from 2016 2016

A WIRELESS SENSOR SYSTEM WITH DIGITALLY CONTROLLED SIGNAL CONDITIONING CIRCUIT FOR FORCE MONITORING AT BONE FIXATION PLATES , Govindan Suresh

DESIGN AND DEVELOPMENT OF OPTICAL ELASTOGRAPHY SETUP , Abhinav Madhavachandran

EFFECTS OF SCATTERING AND ABSORPTION ON LASER SPECKLE CONTRAST IMAGING , Kosar Khaksari

INHIBITION OF BACTERIAL GROWTH AND PREVENTION OF BACTERIAL ADHESION WITH LOCALIZED NITRIC OXIDE DELIVERY , Julia Osborne

WIRELESS IMPLANTABLE MAGNETOELASTIC SENSORS AND ACTUATORS FOR BIOMEDICAL APPLICATIONS , Andrew DeRouin

Wireless Sensor System for Monitoring Strains and Forces On An External Bone Fixation Plate , Sterling Prince

Reports/Theses/Dissertations from 2015 2015

DEVELOPMENT OF A CELL MORPHOLOGICAL ANALYSIS TOOL TO EVALUATE THE ULTRASOUND VIBRATIONAL EFFECTS ON CELL ADHESION , Joseph M. Smith

DEVELOPMENT OF HIGH CAPACITY HYPERBRANCHED NITRIC OXIDE DONORS FOR CONTROLLING SUBCUTANEOUS INFLAMMATION , Sean Hopkins

ENGINEERING APPROACHES FOR SUPPRESSING DELETERIOUS HOST RESPONSES TO MEDICAL IMPLANTS , Connor McCarthy

GELATIN MICROGEL INCORPORATED POLY (ETHYLENE GLYCOL) BIOADHESIVE WITH ENHANCED ADHESIVE PROPERTY AND BIOACTIVITY , Yuting Li

METABOLOMIC AND PROTEOMIC APPROACHES TO UNDERSTAND LEAD STRESS IN VETIVER GRASS (Chrysopogon zizanioides L. NASH) , Venkataramana R. Pidatala

PH RESPONSIVE, ADHESIVE HYDROGELS BASED ON REVERSIBLE CATECHOL - BORONIC ACID COMPLEXATION , Ameya Ravindra Narkar

SYSTEMATIC STUDY OF THE BIOLOGICAL EFFECTS OF NITRIC OXIDE (NO) USING INNOVATIVE NO MEASUREMENT AND DELIVERY SYSTEMS , Weilue He

THE INFLUENCE OF PASSIVE ANKLE JOINT POWER ON BALANCE RECOVERY , Stephanie E. Hamilton

Three-dimensional Mesenchymal Stem Cell Spheroids and Zn-based Biomaterials as Potential Cardiovascular Treatments , Emily Shearier

Reports/Theses/Dissertations from 2014 2014

DESIGN AND APPLICATION OF WIRELESS PASSIVE MAGNETOELASTIC RESONANCE AND MAGNETOHARMONIC FORCE SENSORS , Brandon D. Pereles

Reports/Theses/Dissertations from 2013 2013

Development of Optically Based pH Sensing Hydrogel and Controlled Nitric Oxide Release Polymer , Matthew T. Nielsen

Development of Vapor Deposited Silica Sol-Gel Particles for a Bioactive Materials System to Direct Osteoblast Behavior , Katherine Lynn Snyder

Reports/Theses/Dissertations from 2011 2011

Wireless and passive pressure sensor system based on the magnetic higher-order harmonic field , Ee Lim Tan

Reports/Theses/Dissertations from 2010 2010

Exploration of the role of serum factors in maintaining bone mass during hibernation in black bears , Rachel Marie Bradford

Influence of traumatic impaction and pathological loading on knee menisci , Megan Leigh Killian

Use of a 3D perfusion bioreactor with osteoblasts and osteoblast/endothelial cell co-cultures to improve tissue-engineered bone , Matthew J. Barron

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Graduate Education

Office of graduate and postdoctoral education, phd defense by eunbi park, april 17, 2024.

In partial fulfillment of the requirements for the degree of

Doctor of Philosophy in Bioinformatics

in the School of Biological Sciences

Defends her thesis:

Modeling emergent patterning within pluripotent colonies through Boolean canalizing functions

Wednesday, April 17th, 2024

Krone Engineered Biosystems Building (EBB), Room #5029

Zoom Link: https://gatech.zoom.us/j/98921652004?pwd=QmxVZUNxdVgvMVhYU1BUMGs4alJ0dz09

Meeting ID: 989 2165 2004

Thesis Advisor:

Dr. Melissa L. Kemp

Wallace H. Coulter Department of Biomedical Engineering

Georgia Institute of Technology and Emory University

Committee Members:

Dr. Ahmet F. Coskun

Dr. Elena S. Dimitrova

Mathematics Department

California Polytechnic State University

Dr. Jeffrey T. Streelman

School of Biological Sciences

Georgia Institute of Technology

Dr. Matthew P. Torres

Dr. Denis V. Tsygankov

The early events of human induced pluripotent stem cell (hiPSC) differentiation require collective intercellular communication at both local and long distances for symmetry breaking and pattern formation. It is currently unclear which mechanisms take priority in context-specific situations, and resolving these mechanisms is needed to advance hiPSC-derived tissue engineering. Prior work reported intracellular and intercellular regulation of the FGF/ERK/GATA6 pathway in the mouse blastocyst as a driver of differentiation and self-organization. Computational models capable of simulating intrinsic and/or neighbor-to-neighbor mechanisms provide a useful approach to analyze the role of signaling in multicellular organization during the initial loss of pluripotency.

The objective of this research was to investigate the dynamics of emergent patterning and collective intra-/inter-cellular communication that directs early cell fate decisions in hiPSCs. To enable the interpretation of cellular interactions, a novel pipeline was developed to quantify the differences between spatial organization obtained from both in vitro microscopy images and in silico modeling outcomes using topological data analysis (TDA). Next, I performed characterization of FGF/ERK/GATA6 pathway during emergent pattern formation under multiple molecular perturbations, elucidating context-specific attributes in two-dimensional and three-dimensional culture conditions. Finally, a Python agent-based model of hiPSC colony patterning was created with Boolean networks to discriminate between putative mechanisms of cell fate decisions and compared experimentally observed patterns via TDA. Overall, this work demonstrates that the strategies of quantifying spatial organization and modeling and testing engineered hiPSCs in colonies and organoids are tractable alternative approaches to embryonic systems, allowing for the identification of modes of intercellular communication that determine cell fates.

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