hypothesis 3

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Biology library

Course: biology library   >   unit 1, the scientific method.

• Controlled experiments
• The scientific method and experimental design

Introduction

• Make an observation.
• Ask a question.
• Form a hypothesis , or testable explanation.
• Make a prediction based on the hypothesis.
• Test the prediction.
• Iterate: use the results to make new hypotheses or predictions.

Scientific method example: Failure to toast

1. make an observation..

• Observation: the toaster won't toast.

2. Ask a question.

• Question: Why won't my toaster toast?

3. Propose a hypothesis.

• Hypothesis: Maybe the outlet is broken.

4. Make predictions.

• Prediction: If I plug the toaster into a different outlet, then it will toast the bread.

5. Test the predictions.

• Test of prediction: Plug the toaster into a different outlet and try again.
• If the toaster does toast, then the hypothesis is supported—likely correct.
• If the toaster doesn't toast, then the hypothesis is not supported—likely wrong.

Logical possibility

Practical possibility, building a body of evidence, 6. iterate..

• Iteration time!
• If the hypothesis was supported, we might do additional tests to confirm it, or revise it to be more specific. For instance, we might investigate why the outlet is broken.
• If the hypothesis was not supported, we would come up with a new hypothesis. For instance, the next hypothesis might be that there's a broken wire in the toaster.

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How to Write a Great Hypothesis

Hypothesis Format, Examples, and Tips

Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

Amy Morin, LCSW, is a psychotherapist and international bestselling author. Her books, including "13 Things Mentally Strong People Don't Do," have been translated into more than 40 languages. Her TEDx talk,  "The Secret of Becoming Mentally Strong," is one of the most viewed talks of all time.

Verywell / Alex Dos Diaz

• The Scientific Method

Hypothesis Format

Falsifiability of a hypothesis, operational definitions, types of hypotheses, hypotheses examples.

• Collecting Data

Frequently Asked Questions

A hypothesis is a tentative statement about the relationship between two or more  variables. It is a specific, testable prediction about what you expect to happen in a study.

One hypothesis example would be a study designed to look at the relationship between sleep deprivation and test performance might have a hypothesis that states: "This study is designed to assess the hypothesis that sleep-deprived people will perform worse on a test than individuals who are not sleep-deprived."

This article explores how a hypothesis is used in psychology research, how to write a good hypothesis, and the different types of hypotheses you might use.

The Hypothesis in the Scientific Method

In the scientific method , whether it involves research in psychology, biology, or some other area, a hypothesis represents what the researchers think will happen in an experiment. The scientific method involves the following steps:

• Forming a question
• Performing background research
• Creating a hypothesis
• Designing an experiment
• Collecting data
• Analyzing the results
• Drawing conclusions
• Communicating the results

The hypothesis is a prediction, but it involves more than a guess. Most of the time, the hypothesis begins with a question which is then explored through background research. It is only at this point that researchers begin to develop a testable hypothesis. Unless you are creating an exploratory study, your hypothesis should always explain what you  expect  to happen.

In a study exploring the effects of a particular drug, the hypothesis might be that researchers expect the drug to have some type of effect on the symptoms of a specific illness. In psychology, the hypothesis might focus on how a certain aspect of the environment might influence a particular behavior.

Remember, a hypothesis does not have to be correct. While the hypothesis predicts what the researchers expect to see, the goal of the research is to determine whether this guess is right or wrong. When conducting an experiment, researchers might explore a number of factors to determine which ones might contribute to the ultimate outcome.

In many cases, researchers may find that the results of an experiment  do not  support the original hypothesis. When writing up these results, the researchers might suggest other options that should be explored in future studies.

In many cases, researchers might draw a hypothesis from a specific theory or build on previous research. For example, prior research has shown that stress can impact the immune system. So a researcher might hypothesize: "People with high-stress levels will be more likely to contract a common cold after being exposed to the virus than people who have low-stress levels."

In other instances, researchers might look at commonly held beliefs or folk wisdom. "Birds of a feather flock together" is one example of folk wisdom that a psychologist might try to investigate. The researcher might pose a specific hypothesis that "People tend to select romantic partners who are similar to them in interests and educational level."

Elements of a Good Hypothesis

So how do you write a good hypothesis? When trying to come up with a hypothesis for your research or experiments, ask yourself the following questions:

• Is your hypothesis based on your research on a topic?
• Can your hypothesis be tested?
• Does your hypothesis include independent and dependent variables?

Before you come up with a specific hypothesis, spend some time doing background research. Once you have completed a literature review, start thinking about potential questions you still have. Pay attention to the discussion section in the  journal articles you read . Many authors will suggest questions that still need to be explored.

To form a hypothesis, you should take these steps:

• Collect as many observations about a topic or problem as you can.
• Evaluate these observations and look for possible causes of the problem.
• Create a list of possible explanations that you might want to explore.
• After you have developed some possible hypotheses, think of ways that you could confirm or disprove each hypothesis through experimentation. This is known as falsifiability.

In the scientific method ,  falsifiability is an important part of any valid hypothesis.   In order to test a claim scientifically, it must be possible that the claim could be proven false.

Students sometimes confuse the idea of falsifiability with the idea that it means that something is false, which is not the case. What falsifiability means is that  if  something was false, then it is possible to demonstrate that it is false.

One of the hallmarks of pseudoscience is that it makes claims that cannot be refuted or proven false.

A variable is a factor or element that can be changed and manipulated in ways that are observable and measurable. However, the researcher must also define how the variable will be manipulated and measured in the study.

For example, a researcher might operationally define the variable " test anxiety " as the results of a self-report measure of anxiety experienced during an exam. A "study habits" variable might be defined by the amount of studying that actually occurs as measured by time.

These precise descriptions are important because many things can be measured in a number of different ways. One of the basic principles of any type of scientific research is that the results must be replicable.   By clearly detailing the specifics of how the variables were measured and manipulated, other researchers can better understand the results and repeat the study if needed.

Some variables are more difficult than others to define. How would you operationally define a variable such as aggression ? For obvious ethical reasons, researchers cannot create a situation in which a person behaves aggressively toward others.

In order to measure this variable, the researcher must devise a measurement that assesses aggressive behavior without harming other people. In this situation, the researcher might utilize a simulated task to measure aggressiveness.

Hypothesis Checklist

• Does your hypothesis focus on something that you can actually test?
• Does your hypothesis include both an independent and dependent variable?
• Can you manipulate the variables?
• Can your hypothesis be tested without violating ethical standards?

The hypothesis you use will depend on what you are investigating and hoping to find. Some of the main types of hypotheses that you might use include:

• Simple hypothesis : This type of hypothesis suggests that there is a relationship between one independent variable and one dependent variable.
• Complex hypothesis : This type of hypothesis suggests a relationship between three or more variables, such as two independent variables and a dependent variable.
• Null hypothesis : This hypothesis suggests no relationship exists between two or more variables.
• Alternative hypothesis : This hypothesis states the opposite of the null hypothesis.
• Statistical hypothesis : This hypothesis uses statistical analysis to evaluate a representative sample of the population and then generalizes the findings to the larger group.
• Logical hypothesis : This hypothesis assumes a relationship between variables without collecting data or evidence.

A hypothesis often follows a basic format of "If {this happens} then {this will happen}." One way to structure your hypothesis is to describe what will happen to the  dependent variable  if you change the  independent variable .

The basic format might be: "If {these changes are made to a certain independent variable}, then we will observe {a change in a specific dependent variable}."

A few examples of simple hypotheses:

• "Students who eat breakfast will perform better on a math exam than students who do not eat breakfast."
• Complex hypothesis: "Students who experience test anxiety before an English exam will get lower scores than students who do not experience test anxiety."​
• "Motorists who talk on the phone while driving will be more likely to make errors on a driving course than those who do not talk on the phone."

Examples of a complex hypothesis include:

• "People with high-sugar diets and sedentary activity levels are more likely to develop depression."
• "Younger people who are regularly exposed to green, outdoor areas have better subjective well-being than older adults who have limited exposure to green spaces."

Examples of a null hypothesis include:

• "Children who receive a new reading intervention will have scores different than students who do not receive the intervention."
• "There will be no difference in scores on a memory recall task between children and adults."

Examples of an alternative hypothesis:

• "Children who receive a new reading intervention will perform better than students who did not receive the intervention."
• "Adults will perform better on a memory task than children." 

Collecting Data on Your Hypothesis

Once a researcher has formed a testable hypothesis, the next step is to select a research design and start collecting data. The research method depends largely on exactly what they are studying. There are two basic types of research methods: descriptive research and experimental research.

Descriptive Research Methods

Descriptive research such as  case studies ,  naturalistic observations , and surveys are often used when it would be impossible or difficult to  conduct an experiment . These methods are best used to describe different aspects of a behavior or psychological phenomenon.

Once a researcher has collected data using descriptive methods, a correlational study can then be used to look at how the variables are related. This type of research method might be used to investigate a hypothesis that is difficult to test experimentally.

Experimental Research Methods

Experimental methods  are used to demonstrate causal relationships between variables. In an experiment, the researcher systematically manipulates a variable of interest (known as the independent variable) and measures the effect on another variable (known as the dependent variable).

Unlike correlational studies, which can only be used to determine if there is a relationship between two variables, experimental methods can be used to determine the actual nature of the relationship—whether changes in one variable actually  cause  another to change.

A Word From Verywell

The hypothesis is a critical part of any scientific exploration. It represents what researchers expect to find in a study or experiment. In situations where the hypothesis is unsupported by the research, the research still has value. Such research helps us better understand how different aspects of the natural world relate to one another. It also helps us develop new hypotheses that can then be tested in the future.

Some examples of how to write a hypothesis include:

• "Staying up late will lead to worse test performance the next day."
• "People who consume one apple each day will visit the doctor fewer times each year."
• "Breaking study sessions up into three 20-minute sessions will lead to better test results than a single 60-minute study session."

The four parts of a hypothesis are:

• The research question
• The independent variable (IV)
• The dependent variable (DV)
• The proposed relationship between the IV and DV

Castillo M. The scientific method: a need for something better? . AJNR Am J Neuroradiol. 2013;34(9):1669-71. doi:10.3174/ajnr.A3401

Nevid J. Psychology: Concepts and Applications. Wadworth, 2013.

By Kendra Cherry, MSEd Kendra Cherry, MS, is a psychosocial rehabilitation specialist, psychology educator, and author of the "Everything Psychology Book."

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• Knowledge Base
• Methodology
• How to Write a Strong Hypothesis | Guide & Examples

How to Write a Strong Hypothesis | Guide & Examples

Published on 6 May 2022 by Shona McCombes .

A hypothesis is a statement that can be tested by scientific research. If you want to test a relationship between two or more variables, you need to write hypotheses before you start your experiment or data collection.

Table of contents

What is a hypothesis, developing a hypothesis (with example), hypothesis examples, frequently asked questions about writing hypotheses.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess – it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

Variables in hypotheses

Hypotheses propose a relationship between two or more variables . An independent variable is something the researcher changes or controls. A dependent variable is something the researcher observes and measures.

In this example, the independent variable is exposure to the sun – the assumed cause . The dependent variable is the level of happiness – the assumed effect .

Prevent plagiarism, run a free check.

Step 1: ask a question.

Writing a hypothesis begins with a research question that you want to answer. The question should be focused, specific, and researchable within the constraints of your project.

Step 2: Do some preliminary research

Your initial answer to the question should be based on what is already known about the topic. Look for theories and previous studies to help you form educated assumptions about what your research will find.

At this stage, you might construct a conceptual framework to identify which variables you will study and what you think the relationships are between them. Sometimes, you’ll have to operationalise more complex constructs.

Step 3: Formulate your hypothesis

Now you should have some idea of what you expect to find. Write your initial answer to the question in a clear, concise sentence.

Step 4: Refine your hypothesis

You need to make sure your hypothesis is specific and testable. There are various ways of phrasing a hypothesis, but all the terms you use should have clear definitions, and the hypothesis should contain:

• The relevant variables
• The specific group being studied
• The predicted outcome of the experiment or analysis

Step 5: Phrase your hypothesis in three ways

To identify the variables, you can write a simple prediction in if … then form. The first part of the sentence states the independent variable and the second part states the dependent variable.

In academic research, hypotheses are more commonly phrased in terms of correlations or effects, where you directly state the predicted relationship between variables.

If you are comparing two groups, the hypothesis can state what difference you expect to find between them.

Step 6. Write a null hypothesis

If your research involves statistical hypothesis testing , you will also have to write a null hypothesis. The null hypothesis is the default position that there is no association between the variables. The null hypothesis is written as H 0 , while the alternative hypothesis is H 1 or H a .

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis is not just a guess. It should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations, and statistical analysis of data).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (‘ x affects y because …’).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses. In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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McCombes, S. (2022, May 06). How to Write a Strong Hypothesis | Guide & Examples. Scribbr. Retrieved 9 April 2024, from https://www.scribbr.co.uk/research-methods/hypothesis-writing/

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The Craft of Writing a Strong Hypothesis

Table of Contents

Writing a hypothesis is one of the essential elements of a scientific research paper. It needs to be to the point, clearly communicating what your research is trying to accomplish. A blurry, drawn-out, or complexly-structured hypothesis can confuse your readers. Or worse, the editor and peer reviewers.

A captivating hypothesis is not too intricate. This blog will take you through the process so that, by the end of it, you have a better idea of how to convey your research paper's intent in just one sentence.

What is a Hypothesis?

The first step in your scientific endeavor, a hypothesis, is a strong, concise statement that forms the basis of your research. It is not the same as a thesis statement , which is a brief summary of your research paper .

The sole purpose of a hypothesis is to predict your paper's findings, data, and conclusion. It comes from a place of curiosity and intuition . When you write a hypothesis, you're essentially making an educated guess based on scientific prejudices and evidence, which is further proven or disproven through the scientific method.

The reason for undertaking research is to observe a specific phenomenon. A hypothesis, therefore, lays out what the said phenomenon is. And it does so through two variables, an independent and dependent variable.

The independent variable is the cause behind the observation, while the dependent variable is the effect of the cause. A good example of this is “mixing red and blue forms purple.” In this hypothesis, mixing red and blue is the independent variable as you're combining the two colors at your own will. The formation of purple is the dependent variable as, in this case, it is conditional to the independent variable.

Different Types of Hypotheses‌

Types of hypotheses

Some would stand by the notion that there are only two types of hypotheses: a Null hypothesis and an Alternative hypothesis. While that may have some truth to it, it would be better to fully distinguish the most common forms as these terms come up so often, which might leave you out of context.

Apart from Null and Alternative, there are Complex, Simple, Directional, Non-Directional, Statistical, and Associative and casual hypotheses. They don't necessarily have to be exclusive, as one hypothesis can tick many boxes, but knowing the distinctions between them will make it easier for you to construct your own.

1. Null hypothesis

A null hypothesis proposes no relationship between two variables. Denoted by H 0 , it is a negative statement like “Attending physiotherapy sessions does not affect athletes' on-field performance.” Here, the author claims physiotherapy sessions have no effect on on-field performances. Even if there is, it's only a coincidence.

2. Alternative hypothesis

Considered to be the opposite of a null hypothesis, an alternative hypothesis is donated as H1 or Ha. It explicitly states that the dependent variable affects the independent variable. A good  alternative hypothesis example is “Attending physiotherapy sessions improves athletes' on-field performance.” or “Water evaporates at 100 °C. ” The alternative hypothesis further branches into directional and non-directional.

• Directional hypothesis: A hypothesis that states the result would be either positive or negative is called directional hypothesis. It accompanies H1 with either the ‘<' or ‘>' sign.
• Non-directional hypothesis: A non-directional hypothesis only claims an effect on the dependent variable. It does not clarify whether the result would be positive or negative. The sign for a non-directional hypothesis is ‘≠.'

3. Simple hypothesis

A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, “Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking.

4. Complex hypothesis

In contrast to a simple hypothesis, a complex hypothesis implies the relationship between multiple independent and dependent variables. For instance, “Individuals who eat more fruits tend to have higher immunity, lesser cholesterol, and high metabolism.” The independent variable is eating more fruits, while the dependent variables are higher immunity, lesser cholesterol, and high metabolism.

5. Associative and casual hypothesis

Associative and casual hypotheses don't exhibit how many variables there will be. They define the relationship between the variables. In an associative hypothesis, changing any one variable, dependent or independent, affects others. In a casual hypothesis, the independent variable directly affects the dependent.

6. Empirical hypothesis

Also referred to as the working hypothesis, an empirical hypothesis claims a theory's validation via experiments and observation. This way, the statement appears justifiable and different from a wild guess.

Say, the hypothesis is “Women who take iron tablets face a lesser risk of anemia than those who take vitamin B12.” This is an example of an empirical hypothesis where the researcher  the statement after assessing a group of women who take iron tablets and charting the findings.

7. Statistical hypothesis

The point of a statistical hypothesis is to test an already existing hypothesis by studying a population sample. Hypothesis like “44% of the Indian population belong in the age group of 22-27.” leverage evidence to prove or disprove a particular statement.

Characteristics of a Good Hypothesis

Writing a hypothesis is essential as it can make or break your research for you. That includes your chances of getting published in a journal. So when you're designing one, keep an eye out for these pointers:

• A research hypothesis has to be simple yet clear to look justifiable enough.
• It has to be testable — your research would be rendered pointless if too far-fetched into reality or limited by technology.
• It has to be precise about the results —what you are trying to do and achieve through it should come out in your hypothesis.
• A research hypothesis should be self-explanatory, leaving no doubt in the reader's mind.
• If you are developing a relational hypothesis, you need to include the variables and establish an appropriate relationship among them.
• A hypothesis must keep and reflect the scope for further investigations and experiments.

Separating a Hypothesis from a Prediction

Outside of academia, hypothesis and prediction are often used interchangeably. In research writing, this is not only confusing but also incorrect. And although a hypothesis and prediction are guesses at their core, there are many differences between them.

A hypothesis is an educated guess or even a testable prediction validated through research. It aims to analyze the gathered evidence and facts to define a relationship between variables and put forth a logical explanation behind the nature of events.

Predictions are assumptions or expected outcomes made without any backing evidence. They are more fictionally inclined regardless of where they originate from.

For this reason, a hypothesis holds much more weight than a prediction. It sticks to the scientific method rather than pure guesswork. "Planets revolve around the Sun." is an example of a hypothesis as it is previous knowledge and observed trends. Additionally, we can test it through the scientific method.

Whereas "COVID-19 will be eradicated by 2030." is a prediction. Even though it results from past trends, we can't prove or disprove it. So, the only way this gets validated is to wait and watch if COVID-19 cases end by 2030.

Finally, How to Write a Hypothesis

Quick tips on writing a hypothesis

1.  Be clear about your research question

A hypothesis should instantly address the research question or the problem statement. To do so, you need to ask a question. Understand the constraints of your undertaken research topic and then formulate a simple and topic-centric problem. Only after that can you develop a hypothesis and further test for evidence.

2. Carry out a recce

Once you have your research's foundation laid out, it would be best to conduct preliminary research. Go through previous theories, academic papers, data, and experiments before you start curating your research hypothesis. It will give you an idea of your hypothesis's viability or originality.

Making use of references from relevant research papers helps draft a good research hypothesis. SciSpace Discover offers a repository of over 270 million research papers to browse through and gain a deeper understanding of related studies on a particular topic. Additionally, you can use SciSpace Copilot , your AI research assistant, for reading any lengthy research paper and getting a more summarized context of it. A hypothesis can be formed after evaluating many such summarized research papers. Copilot also offers explanations for theories and equations, explains paper in simplified version, allows you to highlight any text in the paper or clip math equations and tables and provides a deeper, clear understanding of what is being said. This can improve the hypothesis by helping you identify potential research gaps.

3. Create a 3-dimensional hypothesis

Variables are an essential part of any reasonable hypothesis. So, identify your independent and dependent variable(s) and form a correlation between them. The ideal way to do this is to write the hypothetical assumption in the ‘if-then' form. If you use this form, make sure that you state the predefined relationship between the variables.

In another way, you can choose to present your hypothesis as a comparison between two variables. Here, you must specify the difference you expect to observe in the results.

4. Write the first draft

Now that everything is in place, it's time to write your hypothesis. For starters, create the first draft. In this version, write what you expect to find from your research.

Clearly separate your independent and dependent variables and the link between them. Don't fixate on syntax at this stage. The goal is to ensure your hypothesis addresses the issue.

5. Proof your hypothesis

After preparing the first draft of your hypothesis, you need to inspect it thoroughly. It should tick all the boxes, like being concise, straightforward, relevant, and accurate. Your final hypothesis has to be well-structured as well.

Research projects are an exciting and crucial part of being a scholar. And once you have your research question, you need a great hypothesis to begin conducting research. Thus, knowing how to write a hypothesis is very important.

Now that you have a firmer grasp on what a good hypothesis constitutes, the different kinds there are, and what process to follow, you will find it much easier to write your hypothesis, which ultimately helps your research.

Now it's easier than ever to streamline your research workflow with SciSpace Discover . Its integrated, comprehensive end-to-end platform for research allows scholars to easily discover, write and publish their research and fosters collaboration.

It includes everything you need, including a repository of over 270 million research papers across disciplines, SEO-optimized summaries and public profiles to show your expertise and experience.

If you found these tips on writing a research hypothesis useful, head over to our blog on Statistical Hypothesis Testing to learn about the top researchers, papers, and institutions in this domain.

Frequently Asked Questions (FAQs)

1. what is the definition of hypothesis.

According to the Oxford dictionary, a hypothesis is defined as “An idea or explanation of something that is based on a few known facts, but that has not yet been proved to be true or correct”.

2. What is an example of hypothesis?

The hypothesis is a statement that proposes a relationship between two or more variables. An example: "If we increase the number of new users who join our platform by 25%, then we will see an increase in revenue."

3. What is an example of null hypothesis?

A null hypothesis is a statement that there is no relationship between two variables. The null hypothesis is written as H0. The null hypothesis states that there is no effect. For example, if you're studying whether or not a particular type of exercise increases strength, your null hypothesis will be "there is no difference in strength between people who exercise and people who don't."

4. What are the types of research?

• Fundamental research

• Applied research

• Qualitative research

• Quantitative research

• Mixed research

• Exploratory research

• Longitudinal research

• Cross-sectional research

• Field research

• Laboratory research

• Fixed research

• Flexible research

• Action research

• Policy research

• Classification research

• Comparative research

• Causal research

• Inductive research

• Deductive research

5. How to write a hypothesis?

• Your hypothesis should be able to predict the relationship and outcome.

• Avoid wordiness by keeping it simple and brief.

• Your hypothesis should contain observable and testable outcomes.

• Your hypothesis should be relevant to the research question.

6. What are the 2 types of hypothesis?

• Null hypotheses are used to test the claim that "there is no difference between two groups of data".

• Alternative hypotheses test the claim that "there is a difference between two data groups".

7. Difference between research question and research hypothesis?

A research question is a broad, open-ended question you will try to answer through your research. A hypothesis is a statement based on prior research or theory that you expect to be true due to your study. Example - Research question: What are the factors that influence the adoption of the new technology? Research hypothesis: There is a positive relationship between age, education and income level with the adoption of the new technology.

8. What is plural for hypothesis?

The plural of hypothesis is hypotheses. Here's an example of how it would be used in a statement, "Numerous well-considered hypotheses are presented in this part, and they are supported by tables and figures that are well-illustrated."

9. What is the red queen hypothesis?

The red queen hypothesis in evolutionary biology states that species must constantly evolve to avoid extinction because if they don't, they will be outcompeted by other species that are evolving. Leigh Van Valen first proposed it in 1973; since then, it has been tested and substantiated many times.

10. Who is known as the father of null hypothesis?

The father of the null hypothesis is Sir Ronald Fisher. He published a paper in 1925 that introduced the concept of null hypothesis testing, and he was also the first to use the term itself.

11. When to reject null hypothesis?

You need to find a significant difference between your two populations to reject the null hypothesis. You can determine that by running statistical tests such as an independent sample t-test or a dependent sample t-test. You should reject the null hypothesis if the p-value is less than 0.05.

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Scientific Method: Step 3: HYPOTHESIS

• Step 1: QUESTION
• Step 2: RESEARCH
• Step 3: HYPOTHESIS
• Step 4: EXPERIMENT
• Step 5: DATA
• Step 6: CONCLUSION

Step 3: State your hypothesis

Now it's time to state your hypothesis . The hypothesis is an educated guess as to what will happen during your experiment. 

The hypothesis is often written using the words "IF" and "THEN." For example, " If I do not study, then I will fail the test." The "if' and "then" statements reflect your independent and dependent variables . 

The hypothesis should relate back to your original question and must be testable .

A word about variables...

Your experiment will include variables to measure and to explain any cause and effect. Below you will find some useful links describing the different types of variables.

• "What are independent and dependent variables" NCES
• [VIDEO] Biology: Independent vs. Dependent Variables (Nucleus Medical Media) Video explaining independent and dependent variables, with examples.

Resource Links

• What is and How to Write a Good Hypothesis in Research? (Elsevier)
• Hypothesis brochure from Penn State/Berks

• << Previous: Step 2: RESEARCH
• Next: Step 4: EXPERIMENT >>
• Last Updated: Jan 26, 2024 10:39 AM
• URL: https://harford.libguides.com/scientific_method

Educational resources and simple solutions for your research journey

What is a Research Hypothesis: How to Write it, Types, and Examples

Any research begins with a research question and a research hypothesis . A research question alone may not suffice to design the experiment(s) needed to answer it. A hypothesis is central to the scientific method. But what is a hypothesis ? A hypothesis is a testable statement that proposes a possible explanation to a phenomenon, and it may include a prediction. Next, you may ask what is a research hypothesis ? Simply put, a research hypothesis is a prediction or educated guess about the relationship between the variables that you want to investigate.  

It is important to be thorough when developing your research hypothesis. Shortcomings in the framing of a hypothesis can affect the study design and the results. A better understanding of the research hypothesis definition and characteristics of a good hypothesis will make it easier for you to develop your own hypothesis for your research. Let’s dive in to know more about the types of research hypothesis , how to write a research hypothesis , and some research hypothesis examples .  

Table of Contents

What is a hypothesis ?  

A hypothesis is based on the existing body of knowledge in a study area. Framed before the data are collected, a hypothesis states the tentative relationship between independent and dependent variables, along with a prediction of the outcome.  

What is a research hypothesis ?  

Young researchers starting out their journey are usually brimming with questions like “ What is a hypothesis ?” “ What is a research hypothesis ?” “How can I write a good research hypothesis ?”   

A research hypothesis is a statement that proposes a possible explanation for an observable phenomenon or pattern. It guides the direction of a study and predicts the outcome of the investigation. A research hypothesis is testable, i.e., it can be supported or disproven through experimentation or observation.     

Characteristics of a good hypothesis  

Here are the characteristics of a good hypothesis :  

• Clearly formulated and free of language errors and ambiguity  
• Concise and not unnecessarily verbose  
• Has clearly defined variables  
• Testable and stated in a way that allows for it to be disproven  
• Can be tested using a research design that is feasible, ethical, and practical   
• Specific and relevant to the research problem  
• Rooted in a thorough literature search  
• Can generate new knowledge or understanding.  

How to create an effective research hypothesis  

A study begins with the formulation of a research question. A researcher then performs background research. This background information forms the basis for building a good research hypothesis . The researcher then performs experiments, collects, and analyzes the data, interprets the findings, and ultimately, determines if the findings support or negate the original hypothesis.  

Let’s look at each step for creating an effective, testable, and good research hypothesis :  

• Identify a research problem or question: Start by identifying a specific research problem.   
• Review the literature: Conduct an in-depth review of the existing literature related to the research problem to grasp the current knowledge and gaps in the field.   
• Formulate a clear and testable hypothesis : Based on the research question, use existing knowledge to form a clear and testable hypothesis . The hypothesis should state a predicted relationship between two or more variables that can be measured and manipulated. Improve the original draft till it is clear and meaningful.  
• State the null hypothesis: The null hypothesis is a statement that there is no relationship between the variables you are studying.   
• Define the population and sample: Clearly define the population you are studying and the sample you will be using for your research.  
• Select appropriate methods for testing the hypothesis: Select appropriate research methods, such as experiments, surveys, or observational studies, which will allow you to test your research hypothesis .  

Remember that creating a research hypothesis is an iterative process, i.e., you might have to revise it based on the data you collect. You may need to test and reject several hypotheses before answering the research problem.  

How to write a research hypothesis  

When you start writing a research hypothesis , you use an “if–then” statement format, which states the predicted relationship between two or more variables. Clearly identify the independent variables (the variables being changed) and the dependent variables (the variables being measured), as well as the population you are studying. Review and revise your hypothesis as needed.  

An example of a research hypothesis in this format is as follows:  

“ If [athletes] follow [cold water showers daily], then their [endurance] increases.”  

Population: athletes  

Independent variable: daily cold water showers  

Dependent variable: endurance  

You may have understood the characteristics of a good hypothesis . But note that a research hypothesis is not always confirmed; a researcher should be prepared to accept or reject the hypothesis based on the study findings.  

Research hypothesis checklist  

Following from above, here is a 10-point checklist for a good research hypothesis :  

• Testable: A research hypothesis should be able to be tested via experimentation or observation.  
• Specific: A research hypothesis should clearly state the relationship between the variables being studied.  
• Based on prior research: A research hypothesis should be based on existing knowledge and previous research in the field.  
• Falsifiable: A research hypothesis should be able to be disproven through testing.  
• Clear and concise: A research hypothesis should be stated in a clear and concise manner.  
• Logical: A research hypothesis should be logical and consistent with current understanding of the subject.  
• Relevant: A research hypothesis should be relevant to the research question and objectives.  
• Feasible: A research hypothesis should be feasible to test within the scope of the study.  
• Reflects the population: A research hypothesis should consider the population or sample being studied.  
• Uncomplicated: A good research hypothesis is written in a way that is easy for the target audience to understand.  

By following this research hypothesis checklist , you will be able to create a research hypothesis that is strong, well-constructed, and more likely to yield meaningful results.  

Types of research hypothesis  

Different types of research hypothesis are used in scientific research:  

1. Null hypothesis:

A null hypothesis states that there is no change in the dependent variable due to changes to the independent variable. This means that the results are due to chance and are not significant. A null hypothesis is denoted as H0 and is stated as the opposite of what the alternative hypothesis states.   

Example: “ The newly identified virus is not zoonotic .”  

2. Alternative hypothesis:

This states that there is a significant difference or relationship between the variables being studied. It is denoted as H1 or Ha and is usually accepted or rejected in favor of the null hypothesis.  

Example: “ The newly identified virus is zoonotic .”  

3. Directional hypothesis :

This specifies the direction of the relationship or difference between variables; therefore, it tends to use terms like increase, decrease, positive, negative, more, or less.   

Example: “ The inclusion of intervention X decreases infant mortality compared to the original treatment .”   

4. Non-directional hypothesis:

While it does not predict the exact direction or nature of the relationship between the two variables, a non-directional hypothesis states the existence of a relationship or difference between variables but not the direction, nature, or magnitude of the relationship. A non-directional hypothesis may be used when there is no underlying theory or when findings contradict previous research.  

Example, “ Cats and dogs differ in the amount of affection they express .”  

5. Simple hypothesis :

A simple hypothesis only predicts the relationship between one independent and another independent variable.  

Example: “ Applying sunscreen every day slows skin aging .”  

6 . Complex hypothesis :

A complex hypothesis states the relationship or difference between two or more independent and dependent variables.   

Example: “ Applying sunscreen every day slows skin aging, reduces sun burn, and reduces the chances of skin cancer .” (Here, the three dependent variables are slowing skin aging, reducing sun burn, and reducing the chances of skin cancer.)  

7. Associative hypothesis:  

An associative hypothesis states that a change in one variable results in the change of the other variable. The associative hypothesis defines interdependency between variables.  

Example: “ There is a positive association between physical activity levels and overall health .”  

8 . Causal hypothesis:

A causal hypothesis proposes a cause-and-effect interaction between variables.  

Example: “ Long-term alcohol use causes liver damage .”  

Note that some of the types of research hypothesis mentioned above might overlap. The types of hypothesis chosen will depend on the research question and the objective of the study.  

Research hypothesis examples  

Here are some good research hypothesis examples :  

“The use of a specific type of therapy will lead to a reduction in symptoms of depression in individuals with a history of major depressive disorder.”  

“Providing educational interventions on healthy eating habits will result in weight loss in overweight individuals.”  

“Plants that are exposed to certain types of music will grow taller than those that are not exposed to music.”  

“The use of the plant growth regulator X will lead to an increase in the number of flowers produced by plants.”  

Characteristics that make a research hypothesis weak are unclear variables, unoriginality, being too general or too vague, and being untestable. A weak hypothesis leads to weak research and improper methods.   

Some bad research hypothesis examples (and the reasons why they are “bad”) are as follows:  

“This study will show that treatment X is better than any other treatment . ” (This statement is not testable, too broad, and does not consider other treatments that may be effective.)  

“This study will prove that this type of therapy is effective for all mental disorders . ” (This statement is too broad and not testable as mental disorders are complex and different disorders may respond differently to different types of therapy.)  

“Plants can communicate with each other through telepathy . ” (This statement is not testable and lacks a scientific basis.)  

Importance of testable hypothesis  

If a research hypothesis is not testable, the results will not prove or disprove anything meaningful. The conclusions will be vague at best. A testable hypothesis helps a researcher focus on the study outcome and understand the implication of the question and the different variables involved. A testable hypothesis helps a researcher make precise predictions based on prior research.  

To be considered testable, there must be a way to prove that the hypothesis is true or false; further, the results of the hypothesis must be reproducible.  

Frequently Asked Questions (FAQs) on research hypothesis  

1. What is the difference between research question and research hypothesis ?  

A research question defines the problem and helps outline the study objective(s). It is an open-ended statement that is exploratory or probing in nature. Therefore, it does not make predictions or assumptions. It helps a researcher identify what information to collect. A research hypothesis , however, is a specific, testable prediction about the relationship between variables. Accordingly, it guides the study design and data analysis approach.

2. When to reject null hypothesis ?

A null hypothesis should be rejected when the evidence from a statistical test shows that it is unlikely to be true. This happens when the test statistic (e.g., p -value) is less than the defined significance level (e.g., 0.05). Rejecting the null hypothesis does not necessarily mean that the alternative hypothesis is true; it simply means that the evidence found is not compatible with the null hypothesis.  

3. How can I be sure my hypothesis is testable?  

A testable hypothesis should be specific and measurable, and it should state a clear relationship between variables that can be tested with data. To ensure that your hypothesis is testable, consider the following:  

• Clearly define the key variables in your hypothesis. You should be able to measure and manipulate these variables in a way that allows you to test the hypothesis.  
• The hypothesis should predict a specific outcome or relationship between variables that can be measured or quantified.   
• You should be able to collect the necessary data within the constraints of your study.  
• It should be possible for other researchers to replicate your study, using the same methods and variables.   
• Your hypothesis should be testable by using appropriate statistical analysis techniques, so you can draw conclusions, and make inferences about the population from the sample data.  
• The hypothesis should be able to be disproven or rejected through the collection of data.  

4. How do I revise my research hypothesis if my data does not support it?  

If your data does not support your research hypothesis , you will need to revise it or develop a new one. You should examine your data carefully and identify any patterns or anomalies, re-examine your research question, and/or revisit your theory to look for any alternative explanations for your results. Based on your review of the data, literature, and theories, modify your research hypothesis to better align it with the results you obtained. Use your revised hypothesis to guide your research design and data collection. It is important to remain objective throughout the process.  

5. I am performing exploratory research. Do I need to formulate a research hypothesis?  

As opposed to “confirmatory” research, where a researcher has some idea about the relationship between the variables under investigation, exploratory research (or hypothesis-generating research) looks into a completely new topic about which limited information is available. Therefore, the researcher will not have any prior hypotheses. In such cases, a researcher will need to develop a post-hoc hypothesis. A post-hoc research hypothesis is generated after these results are known.  

6. How is a research hypothesis different from a research question?

A research question is an inquiry about a specific topic or phenomenon, typically expressed as a question. It seeks to explore and understand a particular aspect of the research subject. In contrast, a research hypothesis is a specific statement or prediction that suggests an expected relationship between variables. It is formulated based on existing knowledge or theories and guides the research design and data analysis.

7. Can a research hypothesis change during the research process?

Yes, research hypotheses can change during the research process. As researchers collect and analyze data, new insights and information may emerge that require modification or refinement of the initial hypotheses. This can be due to unexpected findings, limitations in the original hypotheses, or the need to explore additional dimensions of the research topic. Flexibility is crucial in research, allowing for adaptation and adjustment of hypotheses to align with the evolving understanding of the subject matter.

8. How many hypotheses should be included in a research study?

The number of research hypotheses in a research study varies depending on the nature and scope of the research. It is not necessary to have multiple hypotheses in every study. Some studies may have only one primary hypothesis, while others may have several related hypotheses. The number of hypotheses should be determined based on the research objectives, research questions, and the complexity of the research topic. It is important to ensure that the hypotheses are focused, testable, and directly related to the research aims.

9. Can research hypotheses be used in qualitative research?

Yes, research hypotheses can be used in qualitative research, although they are more commonly associated with quantitative research. In qualitative research, hypotheses may be formulated as tentative or exploratory statements that guide the investigation. Instead of testing hypotheses through statistical analysis, qualitative researchers may use the hypotheses to guide data collection and analysis, seeking to uncover patterns, themes, or relationships within the qualitative data. The emphasis in qualitative research is often on generating insights and understanding rather than confirming or rejecting specific research hypotheses through statistical testing.

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S.3 hypothesis testing.

In reviewing hypothesis tests, we start first with the general idea. Then, we keep returning to the basic procedures of hypothesis testing, each time adding a little more detail.

The general idea of hypothesis testing involves:

• Making an initial assumption.
• Collecting evidence (data).
• Based on the available evidence (data), deciding whether to reject or not reject the initial assumption.

Every hypothesis test — regardless of the population parameter involved — requires the above three steps.

Example S.3.1

Is normal body temperature really 98.6 degrees f section  .

Consider the population of many, many adults. A researcher hypothesized that the average adult body temperature is lower than the often-advertised 98.6 degrees F. That is, the researcher wants an answer to the question: "Is the average adult body temperature 98.6 degrees? Or is it lower?" To answer his research question, the researcher starts by assuming that the average adult body temperature was 98.6 degrees F.

Then, the researcher went out and tried to find evidence that refutes his initial assumption. In doing so, he selects a random sample of 130 adults. The average body temperature of the 130 sampled adults is 98.25 degrees.

Then, the researcher uses the data he collected to make a decision about his initial assumption. It is either likely or unlikely that the researcher would collect the evidence he did given his initial assumption that the average adult body temperature is 98.6 degrees:

• If it is likely , then the researcher does not reject his initial assumption that the average adult body temperature is 98.6 degrees. There is not enough evidence to do otherwise.
• either the researcher's initial assumption is correct and he experienced a very unusual event;
• or the researcher's initial assumption is incorrect.

In statistics, we generally don't make claims that require us to believe that a very unusual event happened. That is, in the practice of statistics, if the evidence (data) we collected is unlikely in light of the initial assumption, then we reject our initial assumption.

Example S.3.2

Criminal trial analogy section  .

One place where you can consistently see the general idea of hypothesis testing in action is in criminal trials held in the United States. Our criminal justice system assumes "the defendant is innocent until proven guilty." That is, our initial assumption is that the defendant is innocent.

In the practice of statistics, we make our initial assumption when we state our two competing hypotheses -- the null hypothesis ( H 0 ) and the alternative hypothesis ( H A ). Here, our hypotheses are:

• H 0 : Defendant is not guilty (innocent)
• H A : Defendant is guilty

In statistics, we always assume the null hypothesis is true . That is, the null hypothesis is always our initial assumption.

The prosecution team then collects evidence — such as finger prints, blood spots, hair samples, carpet fibers, shoe prints, ransom notes, and handwriting samples — with the hopes of finding "sufficient evidence" to make the assumption of innocence refutable.

In statistics, the data are the evidence.

The jury then makes a decision based on the available evidence:

• If the jury finds sufficient evidence — beyond a reasonable doubt — to make the assumption of innocence refutable, the jury rejects the null hypothesis and deems the defendant guilty. We behave as if the defendant is guilty.
• If there is insufficient evidence, then the jury does not reject the null hypothesis . We behave as if the defendant is innocent.

In statistics, we always make one of two decisions. We either "reject the null hypothesis" or we "fail to reject the null hypothesis."

Errors in Hypothesis Testing Section  

Did you notice the use of the phrase "behave as if" in the previous discussion? We "behave as if" the defendant is guilty; we do not "prove" that the defendant is guilty. And, we "behave as if" the defendant is innocent; we do not "prove" that the defendant is innocent.

This is a very important distinction! We make our decision based on evidence not on 100% guaranteed proof. Again:

• If we reject the null hypothesis, we do not prove that the alternative hypothesis is true.
• If we do not reject the null hypothesis, we do not prove that the null hypothesis is true.

We merely state that there is enough evidence to behave one way or the other. This is always true in statistics! Because of this, whatever the decision, there is always a chance that we made an error .

Let's review the two types of errors that can be made in criminal trials:

Table S.3.2 shows how this corresponds to the two types of errors in hypothesis testing.

Note that, in statistics, we call the two types of errors by two different  names -- one is called a "Type I error," and the other is called  a "Type II error." Here are the formal definitions of the two types of errors:

There is always a chance of making one of these errors. But, a good scientific study will minimize the chance of doing so!

Making the Decision Section  

Recall that it is either likely or unlikely that we would observe the evidence we did given our initial assumption. If it is likely , we do not reject the null hypothesis. If it is unlikely , then we reject the null hypothesis in favor of the alternative hypothesis. Effectively, then, making the decision reduces to determining "likely" or "unlikely."

In statistics, there are two ways to determine whether the evidence is likely or unlikely given the initial assumption:

• We could take the " critical value approach " (favored in many of the older textbooks).
• Or, we could take the " P -value approach " (what is used most often in research, journal articles, and statistical software).

In the next two sections, we review the procedures behind each of these two approaches. To make our review concrete, let's imagine that μ is the average grade point average of all American students who major in mathematics. We first review the critical value approach for conducting each of the following three hypothesis tests about the population mean $\mu$:

In Practice

• We would want to conduct the first hypothesis test if we were interested in concluding that the average grade point average of the group is more than 3.
• We would want to conduct the second hypothesis test if we were interested in concluding that the average grade point average of the group is less than 3.
• And, we would want to conduct the third hypothesis test if we were only interested in concluding that the average grade point average of the group differs from 3 (without caring whether it is more or less than 3).

Upon completing the review of the critical value approach, we review the P -value approach for conducting each of the above three hypothesis tests about the population mean \(\mu\). The procedures that we review here for both approaches easily extend to hypothesis tests about any other population parameter.

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1.1: The Scientific Method

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• Page ID 24080

• Susan Burran and David DesRochers
• Dalton State College via GALILEO Open Learning Materials

Adapted from http://www.biologycorner.com/

Introduction:

The scientific method is central to the study of biology: it is a process of acquiring and verifying information through experimentation. The general steps of the scientific method are depicted in the figure below. The hypothesis , or suggested explanation for the observation, is the basis for setting up experiments. A good experimental design is essential to the scientific method. A few keys to good experimental design include effective use of controls, reproducibility, a large sample size, and multiple trials.

In an experiment, in order to determine that any changes that occur are due to investigator manipulation only, there must be some basis for comparison. A control group is necessary to establish this basis of comparison. In the control group, everything is kept the same as the experimental group except for the independent variable .

The experimental group is actually being experimented upon. For example, in a drug trial, there will be a group that receives the drug (the experimental group) and a group that receives a placebo (the control group). The drug itself is considered the independent variable and any change(s) that occur because of the drug are considered the dependent variable .

In order to ensure that it is only the drug causing changes, all other variables must be tightly controlled (such as diet, exercise, smoking, etc.). These are referred to as controlled variables .

Part 1: The Strange Case of BeriBeri:

In 1887 a strange nerve disease attacked the people in the Dutch East Indies. The disease was beriberi. Symptoms of the disease included weakness and loss of appetite, victims often died of heart failure. Scientists thought the disease might be caused by bacteria. They injected chickens with bacteria from the blood of patients with beriberi. The injected chickens became sick. However, so did a group of chickens that were not injected with bacteria. One of the scientists, Dr. Eijkman, noticed something. Before the experiment, all the chickens had eaten whole-grain rice, but during the experiment, the chickens were fed polished rice. Dr. Eijkman researched this interesting case and found that polished rice lacked thiamine, a vitamin necessary for good health.

1. State the problem.

2. What was the hypothesis?

3. How was the hypothesis tested?

4. Do the results indicate that the hypothesis should be rejected?

5. What should be the new hypothesis and how would you test it?

Part 2: How Penicillin Was Discovered:

In 1928, Sir Alexander Fleming was studying Staphylococcus bacteria growing in culture dishes. He noticed that a mold called Penicillium was also growing in some of the dishes. A clear area existed around the mold because all the bacteria that had grown in this area had died. In the culture dishes without the mold, no clear areas were present. Fleming hypothesized that the mold must be producing a chemical that killed the bacteria. He decided to isolate this substance and test it to see if it would kill bacteria. Fleming transferred the mold to a nutrient broth solution. This solution contained all the materials the mold needed to grow. After the mold grew, he removed it from the nutrient broth. Fleming then added the nutrient broth in which the mold had grown to a culture of bacteria. He observed that the bacteria died which was later used to develop antibiotics used to treat a variety of diseases.

1. Identify the problem.

2. What was Fleming's hypothesis?

5. This experiment led to the development of what major medical advancement…?

Part 3: Identify the Controls and Variables

Smithers thinks that a special juice will increase the productivity of workers. He creates two groups of 50 workers each and assigns each group the same task (in this case, they're supposed to staple a set of papers). Group A is given the special juice to drink while they work. Group B is not given the special juice. After an hour, Smithers counts how many stacks of papers each group has made. Group A made 1,587 stacks; Group B made 2,113 stacks.

Identify the:

• Control Group:
• Independent Variable:
• Dependent Variable:

What should Smithers' conclusion be?

How could this experiment be improved?

Homer notices that his shower is covered in a strange green slime. His friend Barney tells him that coconut juice will get rid of the green slime. Homer decides to check this out by spraying half of the shower with coconut juice. He sprays the other half of the shower with water. After 3 days of "treatment", there is no change in the appearance of the green slime on either side of the shower.

What was the initial observation?

What should Homer's conclusion be?

Bart believes that mice exposed to radio waves will become extra strong (maybe he's been reading too much Radioactive Man). He decides to perform this experiment by placing 10 mice near a radio for 5 hours. He compared these 10 mice to another 10 mice that had not been exposed. His test consisted of a heavy block of wood that blocked the mouse food. He found that 8 out of 10 of the exposed mice were able to push the block away, while 7 out of 10 of the other mice were able to do the same.

What should Bart's conclusion be?

How could Bart's experiment be improved?

Krusty was told that a certain itching powder was the newest best thing on the market: it even claims to cause 50% longer lasting itches. Interested in this product, he buys the itching powder and compares it to his usual product. One test subject (A) is sprinkled with the original itching powder, and another test subject (B) was sprinkled with the Experimental itching powder. Subject A reported having itches for 30 minutes. Subject B reported having itches for 45 minutes

Explain whether the data supports the advertisement's claims about its product.

Design Lisa's experiment.

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Home » What is a Hypothesis – Types, Examples and Writing Guide

What is a Hypothesis – Types, Examples and Writing Guide

Table of Contents

Definition:

Hypothesis is an educated guess or proposed explanation for a phenomenon, based on some initial observations or data. It is a tentative statement that can be tested and potentially proven or disproven through further investigation and experimentation.

Hypothesis is often used in scientific research to guide the design of experiments and the collection and analysis of data. It is an essential element of the scientific method, as it allows researchers to make predictions about the outcome of their experiments and to test those predictions to determine their accuracy.

Types of Hypothesis

Types of Hypothesis are as follows:

Research Hypothesis

A research hypothesis is a statement that predicts a relationship between variables. It is usually formulated as a specific statement that can be tested through research, and it is often used in scientific research to guide the design of experiments.

Null Hypothesis

The null hypothesis is a statement that assumes there is no significant difference or relationship between variables. It is often used as a starting point for testing the research hypothesis, and if the results of the study reject the null hypothesis, it suggests that there is a significant difference or relationship between variables.

Alternative Hypothesis

An alternative hypothesis is a statement that assumes there is a significant difference or relationship between variables. It is often used as an alternative to the null hypothesis and is tested against the null hypothesis to determine which statement is more accurate.

Directional Hypothesis

A directional hypothesis is a statement that predicts the direction of the relationship between variables. For example, a researcher might predict that increasing the amount of exercise will result in a decrease in body weight.

Non-directional Hypothesis

A non-directional hypothesis is a statement that predicts the relationship between variables but does not specify the direction. For example, a researcher might predict that there is a relationship between the amount of exercise and body weight, but they do not specify whether increasing or decreasing exercise will affect body weight.

Statistical Hypothesis

A statistical hypothesis is a statement that assumes a particular statistical model or distribution for the data. It is often used in statistical analysis to test the significance of a particular result.

Composite Hypothesis

A composite hypothesis is a statement that assumes more than one condition or outcome. It can be divided into several sub-hypotheses, each of which represents a different possible outcome.

Empirical Hypothesis

An empirical hypothesis is a statement that is based on observed phenomena or data. It is often used in scientific research to develop theories or models that explain the observed phenomena.

Simple Hypothesis

A simple hypothesis is a statement that assumes only one outcome or condition. It is often used in scientific research to test a single variable or factor.

Complex Hypothesis

A complex hypothesis is a statement that assumes multiple outcomes or conditions. It is often used in scientific research to test the effects of multiple variables or factors on a particular outcome.

Applications of Hypothesis

Hypotheses are used in various fields to guide research and make predictions about the outcomes of experiments or observations. Here are some examples of how hypotheses are applied in different fields:

• Science : In scientific research, hypotheses are used to test the validity of theories and models that explain natural phenomena. For example, a hypothesis might be formulated to test the effects of a particular variable on a natural system, such as the effects of climate change on an ecosystem.
• Medicine : In medical research, hypotheses are used to test the effectiveness of treatments and therapies for specific conditions. For example, a hypothesis might be formulated to test the effects of a new drug on a particular disease.
• Psychology : In psychology, hypotheses are used to test theories and models of human behavior and cognition. For example, a hypothesis might be formulated to test the effects of a particular stimulus on the brain or behavior.
• Sociology : In sociology, hypotheses are used to test theories and models of social phenomena, such as the effects of social structures or institutions on human behavior. For example, a hypothesis might be formulated to test the effects of income inequality on crime rates.
• Business : In business research, hypotheses are used to test the validity of theories and models that explain business phenomena, such as consumer behavior or market trends. For example, a hypothesis might be formulated to test the effects of a new marketing campaign on consumer buying behavior.
• Engineering : In engineering, hypotheses are used to test the effectiveness of new technologies or designs. For example, a hypothesis might be formulated to test the efficiency of a new solar panel design.

How to write a Hypothesis

Here are the steps to follow when writing a hypothesis:

Identify the Research Question

The first step is to identify the research question that you want to answer through your study. This question should be clear, specific, and focused. It should be something that can be investigated empirically and that has some relevance or significance in the field.

Conduct a Literature Review

Before writing your hypothesis, it’s essential to conduct a thorough literature review to understand what is already known about the topic. This will help you to identify the research gap and formulate a hypothesis that builds on existing knowledge.

Determine the Variables

The next step is to identify the variables involved in the research question. A variable is any characteristic or factor that can vary or change. There are two types of variables: independent and dependent. The independent variable is the one that is manipulated or changed by the researcher, while the dependent variable is the one that is measured or observed as a result of the independent variable.

Formulate the Hypothesis

Based on the research question and the variables involved, you can now formulate your hypothesis. A hypothesis should be a clear and concise statement that predicts the relationship between the variables. It should be testable through empirical research and based on existing theory or evidence.

Write the Null Hypothesis

The null hypothesis is the opposite of the alternative hypothesis, which is the hypothesis that you are testing. The null hypothesis states that there is no significant difference or relationship between the variables. It is important to write the null hypothesis because it allows you to compare your results with what would be expected by chance.

Refine the Hypothesis

After formulating the hypothesis, it’s important to refine it and make it more precise. This may involve clarifying the variables, specifying the direction of the relationship, or making the hypothesis more testable.

Examples of Hypothesis

Here are a few examples of hypotheses in different fields:

• Psychology : “Increased exposure to violent video games leads to increased aggressive behavior in adolescents.”
• Biology : “Higher levels of carbon dioxide in the atmosphere will lead to increased plant growth.”
• Sociology : “Individuals who grow up in households with higher socioeconomic status will have higher levels of education and income as adults.”
• Education : “Implementing a new teaching method will result in higher student achievement scores.”
• Marketing : “Customers who receive a personalized email will be more likely to make a purchase than those who receive a generic email.”
• Physics : “An increase in temperature will cause an increase in the volume of a gas, assuming all other variables remain constant.”
• Medicine : “Consuming a diet high in saturated fats will increase the risk of developing heart disease.”

Purpose of Hypothesis

The purpose of a hypothesis is to provide a testable explanation for an observed phenomenon or a prediction of a future outcome based on existing knowledge or theories. A hypothesis is an essential part of the scientific method and helps to guide the research process by providing a clear focus for investigation. It enables scientists to design experiments or studies to gather evidence and data that can support or refute the proposed explanation or prediction.

The formulation of a hypothesis is based on existing knowledge, observations, and theories, and it should be specific, testable, and falsifiable. A specific hypothesis helps to define the research question, which is important in the research process as it guides the selection of an appropriate research design and methodology. Testability of the hypothesis means that it can be proven or disproven through empirical data collection and analysis. Falsifiability means that the hypothesis should be formulated in such a way that it can be proven wrong if it is incorrect.

In addition to guiding the research process, the testing of hypotheses can lead to new discoveries and advancements in scientific knowledge. When a hypothesis is supported by the data, it can be used to develop new theories or models to explain the observed phenomenon. When a hypothesis is not supported by the data, it can help to refine existing theories or prompt the development of new hypotheses to explain the phenomenon.

When to use Hypothesis

Here are some common situations in which hypotheses are used:

• In scientific research , hypotheses are used to guide the design of experiments and to help researchers make predictions about the outcomes of those experiments.
• In social science research , hypotheses are used to test theories about human behavior, social relationships, and other phenomena.
• I n business , hypotheses can be used to guide decisions about marketing, product development, and other areas. For example, a hypothesis might be that a new product will sell well in a particular market, and this hypothesis can be tested through market research.

Characteristics of Hypothesis

Here are some common characteristics of a hypothesis:

• Testable : A hypothesis must be able to be tested through observation or experimentation. This means that it must be possible to collect data that will either support or refute the hypothesis.
• Falsifiable : A hypothesis must be able to be proven false if it is not supported by the data. If a hypothesis cannot be falsified, then it is not a scientific hypothesis.
• Clear and concise : A hypothesis should be stated in a clear and concise manner so that it can be easily understood and tested.
• Based on existing knowledge : A hypothesis should be based on existing knowledge and research in the field. It should not be based on personal beliefs or opinions.
• Specific : A hypothesis should be specific in terms of the variables being tested and the predicted outcome. This will help to ensure that the research is focused and well-designed.
• Tentative: A hypothesis is a tentative statement or assumption that requires further testing and evidence to be confirmed or refuted. It is not a final conclusion or assertion.
• Relevant : A hypothesis should be relevant to the research question or problem being studied. It should address a gap in knowledge or provide a new perspective on the issue.

Advantages of Hypothesis

Hypotheses have several advantages in scientific research and experimentation:

• Guides research: A hypothesis provides a clear and specific direction for research. It helps to focus the research question, select appropriate methods and variables, and interpret the results.
• Predictive powe r: A hypothesis makes predictions about the outcome of research, which can be tested through experimentation. This allows researchers to evaluate the validity of the hypothesis and make new discoveries.
• Facilitates communication: A hypothesis provides a common language and framework for scientists to communicate with one another about their research. This helps to facilitate the exchange of ideas and promotes collaboration.
• Efficient use of resources: A hypothesis helps researchers to use their time, resources, and funding efficiently by directing them towards specific research questions and methods that are most likely to yield results.
• Provides a basis for further research: A hypothesis that is supported by data provides a basis for further research and exploration. It can lead to new hypotheses, theories, and discoveries.
• Increases objectivity: A hypothesis can help to increase objectivity in research by providing a clear and specific framework for testing and interpreting results. This can reduce bias and increase the reliability of research findings.

Limitations of Hypothesis

Some Limitations of the Hypothesis are as follows:

• Limited to observable phenomena: Hypotheses are limited to observable phenomena and cannot account for unobservable or intangible factors. This means that some research questions may not be amenable to hypothesis testing.
• May be inaccurate or incomplete: Hypotheses are based on existing knowledge and research, which may be incomplete or inaccurate. This can lead to flawed hypotheses and erroneous conclusions.
• May be biased: Hypotheses may be biased by the researcher’s own beliefs, values, or assumptions. This can lead to selective interpretation of data and a lack of objectivity in research.
• Cannot prove causation: A hypothesis can only show a correlation between variables, but it cannot prove causation. This requires further experimentation and analysis.
• Limited to specific contexts: Hypotheses are limited to specific contexts and may not be generalizable to other situations or populations. This means that results may not be applicable in other contexts or may require further testing.
• May be affected by chance : Hypotheses may be affected by chance or random variation, which can obscure or distort the true relationship between variables.

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Writing a Hypothesis for Your Science Fair Project

What is a hypothesis.

Once a scientist has a scientific question she is interested in, the scientist reads up to find out what is already known on the topic. Then she uses that information to form a tentative answer to her scientific question. Sometimes people refer to the tentative answer as "an educated guess." Keep in mind, though, that the hypothesis also has to be testable since the next step is to do an experiment to determine whether or not the hypothesis is right!

A hypothesis leads to one or more predictions that can be tested by experimenting.

Predictions often take the shape of "If ____then ____" statements, but do not have to. Predictions should include both an independent variable (the factor you change in an experiment) and a dependent variable (the factor you observe or measure in an experiment). A single hypothesis can lead to multiple predictions, but generally, one or two predictions is enough to tackle for a science fair project.

Examples of Hypotheses and Predictions

What if my hypothesis is wrong.

What happens if, at the end of your science project, you look at the data you have collected and you realize it does not support your hypothesis? First, do not panic! The point of a science project is not to prove your hypothesis right. The point is to understand more about how the natural world works. Or, as it is sometimes put, to find out the scientific truth. When scientists do an experiment, they very often have data that shows their starting hypothesis was wrong. Why? Well, the natural world is complex—it takes a lot of experimenting to figure out how it works—and the more explanations you test, the closer you get to figuring out the truth. For scientists, disproving a hypothesis still means they gained important information, and they can use that information to make their next hypothesis even better. In a science fair setting, judges can be just as impressed by projects that start out with a faulty hypothesis; what matters more is whether you understood your science fair project, had a well-controlled experiment, and have ideas about what you would do next to improve your project if you had more time. You can read more about a science fair judge's view on disproving your hypothesis at Learn More About the Scientific Method .

It is worth noting, scientists never talk about their hypothesis being "right" or "wrong." Instead, they say that their data "supports" or "does not support" their hypothesis. This goes back to the point that nature is complex—so complex that it takes more than a single experiment to figure it all out because a single experiment could give you misleading data. For example, let us say that you hypothesize that earthworms do not exist in places that have very cold winters because it is too cold for them to survive. You then predict that you will find earthworms in the dirt in Florida, which has warm winters, but not Alaska, which has cold winters. When you go and dig a 3-foot by 3-foot-wide and 1-foot-deep hole in the dirt in those two states, you discover Floridian earthworms, but not Alaskan ones. So, was your hypothesis right? Well, your data "supported" your hypothesis, but your experiment did not cover that much ground. Can you really be sure there are no earthworms in Alaska? No. Which is why scientists only support (or not) their hypothesis with data, rather than proving them. And for the curious, yes there are earthworms in Alaska .

What Makes a Good Hypothesis?

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A hypothesis is an explanation for a set of observations. Here are examples of a scientific hypothesis.

Although you could state a scientific hypothesis in various ways, most hypotheses are either "If, then" statements or forms of the null hypothesis . The null hypothesis is sometimes called the "no difference" hypothesis. The null hypothesis is good for experimentation because it's simple to disprove. If you disprove a null hypothesis, that is evidence for a relationship between the variables you are examining.

Examples of Null Hypotheses

• Hyperactivity is unrelated to eating sugar.
• All daisies have the same number of petals.
• The number of pets in a household is unrelated to the number of people living in it.
• A person's preference for a shirt is unrelated to its color.

Examples of If, Then Hypotheses

• If you get at least 6 hours of sleep, you will do better on tests than if you get less sleep.
• If you drop a ball, it will fall toward the ground.
• If you drink coffee before going to bed, then it will take longer to fall asleep.
• If you cover a wound with a bandage, then it will heal with less scarring.

Improving a Hypothesis to Make It Testable

You may wish to revise your first hypothesis in order to make it easier to design an experiment to test. For example, let's say you have a bad breakout the morning after eating a lot of greasy food. You may wonder if there is a correlation between eating greasy food and getting pimples. You propose the hypothesis:

Eating greasy food causes pimples.

Next, you need to design an experiment to test this hypothesis. Let's say you decide to eat greasy food every day for a week and record the effect on your face. Then, as a control, you'll avoid greasy food for the next week and see what happens. Now, this is not a good experiment because it does not take into account other factors such as hormone levels, stress, sun exposure, exercise, or any number of other variables that might conceivably affect your skin.

The problem is that you cannot assign cause to your effect . If you eat french fries for a week and suffer a breakout, can you definitely say it was the grease in the food that caused it? Maybe it was the salt. Maybe it was the potato. Maybe it was unrelated to diet. You can't prove your hypothesis. It's much easier to disprove a hypothesis.

So, let's restate the hypothesis to make it easier to evaluate the data:

Getting pimples is unaffected by eating greasy food.

So, if you eat fatty food every day for a week and suffer breakouts and then don't break out the week that you avoid greasy food, you can be pretty sure something is up. Can you disprove the hypothesis? Probably not, since it is so hard to assign cause and effect. However, you can make a strong case that there is some relationship between diet and acne.

If your skin stays clear for the entire test, you may decide to accept your hypothesis . Again, you didn't prove or disprove anything, which is fine

• Null Hypothesis Definition and Examples
• What Is a Hypothesis? (Science)
• What Are the Elements of a Good Hypothesis?
• Understanding Simple vs Controlled Experiments
• What Is a Testable Hypothesis?
• What 'Fail to Reject' Means in a Hypothesis Test
• Null Hypothesis Examples
• How To Design a Science Fair Experiment
• Scientific Method Vocabulary Terms
• Scientific Hypothesis Examples
• Six Steps of the Scientific Method
• An Example of a Hypothesis Test
• Definition of a Hypothesis
• Scientific Method Flow Chart
• Null Hypothesis and Alternative Hypothesis

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How to Write a Hypothesis

Last Updated: May 2, 2023 Fact Checked

This article was co-authored by Bess Ruff, MA . Bess Ruff is a Geography PhD student at Florida State University. She received her MA in Environmental Science and Management from the University of California, Santa Barbara in 2016. She has conducted survey work for marine spatial planning projects in the Caribbean and provided research support as a graduate fellow for the Sustainable Fisheries Group. There are 9 references cited in this article, which can be found at the bottom of the page. This article has been fact-checked, ensuring the accuracy of any cited facts and confirming the authority of its sources. This article has been viewed 1,032,288 times.

A hypothesis is a description of a pattern in nature or an explanation about some real-world phenomenon that can be tested through observation and experimentation. The most common way a hypothesis is used in scientific research is as a tentative, testable, and falsifiable statement that explains some observed phenomenon in nature. [1] X Research source Many academic fields, from the physical sciences to the life sciences to the social sciences, use hypothesis testing as a means of testing ideas to learn about the world and advance scientific knowledge. Whether you are a beginning scholar or a beginning student taking a class in a science subject, understanding what hypotheses are and being able to generate hypotheses and predictions yourself is very important. These instructions will help get you started.

Preparing to Write a Hypothesis

• If you are writing a hypothesis for a school assignment, this step may be taken care of for you.

• Focus on academic and scholarly writing. You need to be certain that your information is unbiased, accurate, and comprehensive. Scholarly search databases such as Google Scholar and Web of Science can help you find relevant articles from reputable sources.
• You can find information in textbooks, at a library, and online. If you are in school, you can also ask for help from teachers, librarians, and your peers.

• For example, if you are interested in the effects of caffeine on the human body, but notice that nobody seems to have explored whether caffeine affects males differently than it does females, this could be something to formulate a hypothesis about. Or, if you are interested in organic farming, you might notice that no one has tested whether organic fertilizer results in different growth rates for plants than non-organic fertilizer.
• You can sometimes find holes in the existing literature by looking for statements like “it is unknown” in scientific papers or places where information is clearly missing. You might also find a claim in the literature that seems far-fetched, unlikely, or too good to be true, like that caffeine improves math skills. If the claim is testable, you could provide a great service to scientific knowledge by doing your own investigation. If you confirm the claim, the claim becomes even more credible. If you do not find support for the claim, you are helping with the necessary self-correcting aspect of science.
• Examining these types of questions provides an excellent way for you to set yourself apart by filling in important gaps in a field of study.

• Following the examples above, you might ask: "How does caffeine affect females as compared to males?" or "How does organic fertilizer affect plant growth compared to non-organic fertilizer?" The rest of your research will be aimed at answering these questions.

• Following the examples above, if you discover in the literature that there is a pattern that some other types of stimulants seem to affect females more than males, this could be a clue that the same pattern might be true for caffeine. Similarly, if you observe the pattern that organic fertilizer seems to be associated with smaller plants overall, you might explain this pattern with the hypothesis that plants exposed to organic fertilizer grow more slowly than plants exposed to non-organic fertilizer.

Formulating Your Hypothesis

• You can think of the independent variable as the one that is causing some kind of difference or effect to occur. In the examples, the independent variable would be biological sex, i.e. whether a person is male or female, and fertilizer type, i.e. whether the fertilizer is organic or non-organically-based.
• The dependent variable is what is affected by (i.e. "depends" on) the independent variable. In the examples above, the dependent variable would be the measured impact of caffeine or fertilizer.
• Your hypothesis should only suggest one relationship. Most importantly, it should only have one independent variable. If you have more than one, you won't be able to determine which one is actually the source of any effects you might observe.

• Don't worry too much at this point about being precise or detailed.
• In the examples above, one hypothesis would make a statement about whether a person's biological sex might impact the way the person is affected by caffeine; for example, at this point, your hypothesis might simply be: "a person's biological sex is related to how caffeine affects his or her heart rate." The other hypothesis would make a general statement about plant growth and fertilizer; for example your simple explanatory hypothesis might be "plants given different types of fertilizer are different sizes because they grow at different rates."

• Using our example, our non-directional hypotheses would be "there is a relationship between a person's biological sex and how much caffeine increases the person's heart rate," and "there is a relationship between fertilizer type and the speed at which plants grow."
• Directional predictions using the same example hypotheses above would be : "Females will experience a greater increase in heart rate after consuming caffeine than will males," and "plants fertilized with non-organic fertilizer will grow faster than those fertilized with organic fertilizer." Indeed, these predictions and the hypotheses that allow for them are very different kinds of statements. More on this distinction below.
• If the literature provides any basis for making a directional prediction, it is better to do so, because it provides more information. Especially in the physical sciences, non-directional predictions are often seen as inadequate.

• Where necessary, specify the population (i.e. the people or things) about which you hope to uncover new knowledge. For example, if you were only interested the effects of caffeine on elderly people, your prediction might read: "Females over the age of 65 will experience a greater increase in heart rate than will males of the same age." If you were interested only in how fertilizer affects tomato plants, your prediction might read: "Tomato plants treated with non-organic fertilizer will grow faster in the first three months than will tomato plants treated with organic fertilizer."

• For example, you would not want to make the hypothesis: "red is the prettiest color." This statement is an opinion and it cannot be tested with an experiment. However, proposing the generalizing hypothesis that red is the most popular color is testable with a simple random survey. If you do indeed confirm that red is the most popular color, your next step may be to ask: Why is red the most popular color? The answer you propose is your explanatory hypothesis .

• An easy way to get to the hypothesis for this method and prediction is to ask yourself why you think heart rates will increase if children are given caffeine. Your explanatory hypothesis in this case may be that caffeine is a stimulant. At this point, some scientists write a research hypothesis , a statement that includes the hypothesis, the experiment, and the prediction all in one statement.
• For example, If caffeine is a stimulant, and some children are given a drink with caffeine while others are given a drink without caffeine, then the heart rates of those children given a caffeinated drink will increase more than the heart rate of children given a non-caffeinated drink.

• Using the above example, if you were to test the effects of caffeine on the heart rates of children, evidence that your hypothesis is not true, sometimes called the null hypothesis , could occur if the heart rates of both the children given the caffeinated drink and the children given the non-caffeinated drink (called the placebo control) did not change, or lowered or raised with the same magnitude, if there was no difference between the two groups of children.
• It is important to note here that the null hypothesis actually becomes much more useful when researchers test the significance of their results with statistics. When statistics are used on the results of an experiment, a researcher is testing the idea of the null statistical hypothesis. For example, that there is no relationship between two variables or that there is no difference between two groups. [8] X Research source

Hypothesis Examples

Community Q&A

• Remember that science is not necessarily a linear process and can be approached in various ways. [10] X Research source Thanks Helpful 0 Not Helpful 0
• When examining the literature, look for research that is similar to what you want to do, and try to build on the findings of other researchers. But also look for claims that you think are suspicious, and test them yourself. Thanks Helpful 0 Not Helpful 0
• Be specific in your hypotheses, but not so specific that your hypothesis can't be applied to anything outside your specific experiment. You definitely want to be clear about the population about which you are interested in drawing conclusions, but nobody (except your roommates) will be interested in reading a paper with the prediction: "my three roommates will each be able to do a different amount of pushups." Thanks Helpful 0 Not Helpful 0

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• ↑ https://undsci.berkeley.edu/for-educators/prepare-and-plan/correcting-misconceptions/#a4
• ↑ https://owl.purdue.edu/owl/general_writing/common_writing_assignments/research_papers/choosing_a_topic.html
• ↑ https://owl.purdue.edu/owl/subject_specific_writing/writing_in_the_social_sciences/writing_in_psychology_experimental_report_writing/experimental_reports_1.html
• ↑ https://www.grammarly.com/blog/how-to-write-a-hypothesis/
• ↑ https://grammar.yourdictionary.com/for-students-and-parents/how-create-hypothesis.html
• ↑ https://flexbooks.ck12.org/cbook/ck-12-middle-school-physical-science-flexbook-2.0/section/1.19/primary/lesson/hypothesis-ms-ps/
• ↑ https://iastate.pressbooks.pub/preparingtopublish/chapter/goal-1-contextualize-the-studys-methods/
• ↑ http://mathworld.wolfram.com/NullHypothesis.html
• ↑ http://undsci.berkeley.edu/article/scienceflowchart

About This Article

Before writing a hypothesis, think of what questions are still unanswered about a specific subject and make an educated guess about what the answer could be. Then, determine the variables in your question and write a simple statement about how they might be related. Try to focus on specific predictions and variables, such as age or segment of the population, to make your hypothesis easier to test. For tips on how to test your hypothesis, read on! Did this summary help you? Yes No

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Three Famous Hypotheses and How They Were Tested

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Key Takeaways

• Ivan Pavlov's experiment demonstrated conditioned responses in dogs.
• Pavlov's work exemplifies the scientific method, starting with a hypothesis about conditioned responses and testing it through controlled experiments.
• Pavlov's findings not only advanced an understanding of animal physiology but also laid foundational principles for behaviorism, a major school of thought in psychology that emphasizes the study of observable behaviors.

Coho salmon ( Oncorhynchus kisutch ) are amazing fish. Indigenous to the Pacific Northwest, they begin their lives in freshwater streams and then relocate to the open ocean. But when a Coho salmon reaches breeding age, it'll return to the waterway of its birth , sometimes traveling 400 miles (644 kilometers) to get there.

Enter the late Arthur Davis Hasler. While an ecologist and biologist at the University of Wisconsin, he was intrigued by the question of how these creatures find their home streams. And in 1960, he used a Hypothesis-Presentation.pdf">basic tenet of science — the hypothesis — to find out.

So what is a hypothesis? A hypothesis is a tentative, testable explanation for an observed phenomenon in nature. Hypotheses are narrow in scope — unlike theories , which cover a broad range of observable phenomena and draw from many different lines of evidence. Meanwhile, a prediction is a result you'd expect to get if your hypothesis or theory is accurate.

So back to 1960 and Hasler and those salmon. One unverified idea was that Coho salmon used eyesight to locate their home streams. Hasler set out to test this notion (or hypothesis). First, he rounded up several fish who'd already returned to their native streams. Next, he blindfolded some of the captives — but not all of them — before dumping his salmon into a faraway stretch of water. If the eyesight hypothesis was correct, then Hasler could expect fewer of the blindfolded fish to return to their home streams.

Things didn't work out that way. The fish without blindfolds came back at the same rate as their blindfolded counterparts. (Other experiments demonstrated that smell, and not sight, is the key to the species' homing ability.)

Although Hasler's blindfold hypothesis was disproven, others have fared better. Today, we're looking at three of the best-known experiments in history — and the hypotheses they tested.

Ivan Pavlov and His Dogs (1903-1935)

Isaac newton's radiant prisms (1665), robert paine's revealing starfish (1963-1969).

The Hypothesis : If dogs are susceptible to conditioned responses (drooling), then a dog who is regularly exposed to the same neutral stimulus (metronome/bell) before it receives food will associate this neutral stimulus with the act of eating. Eventually, the dog should begin to drool at a predictable rate when it encounters said stimulus — even before any actual food is offered.

The Experiment : A Nobel Prize-winner and outspoken critic of Soviet communism, Ivan Pavlov is synonymous with man's best friend . In 1903, the Russian-born scientist kicked off a decades-long series of experiments involving dogs and conditioned responses .

Offer a plate of food to a hungry dog and it'll salivate. In this context, the stimulus (the food) will automatically trigger a particular response (the drooling). The latter is an innate, unlearned reaction to the former.

By contrast, the rhythmic sound of a metronome or bell is a neutral stimulus. To a dog, the noise has no inherent meaning and if the animal has never heard it before, the sound won't provoke an instinctive reaction. But the sight of food sure will .

So when Pavlov and his lab assistants played the sound of the metronome/bell before feeding sessions, the researchers conditioned test dogs to mentally link metronomes/bells with mealtime. Due to repeated exposure, the noise alone started to make the dogs' mouths water before they were given food.

According to " Ivan Pavlov: A Russian Life in Science " by biographer Daniel P. Todes, Pavlov's big innovation here was his discovery that he could quantify the reaction of each pooch by measuring the amount of saliva it generated. Every canine predictably drooled at its own consistent rate when he or she encountered a personalized (and artificial) food-related cue.

Pavlov and his assistants used conditioned responses to look at other hypotheses about animal physiology, as well. In one notable experiment, a dog was tested on its ability to tell time . This particular pooch always received food when it heard a metronome click at the rate of 60 strokes per minute. But it never got any food after listening to a slower, 40-strokes-per-minute beat. Lo and behold, Pavlov's animal began to salivate in response to the faster rhythm — but not the slower one . So clearly, it could tell the two rhythmic beats apart.

The Verdict : With the right conditioning — and lots of patience — you can make a hungry dog respond to neutral stimuli by salivating on cue in a way that's both predictable and scientifically quantifiable.

The Hypothesis : If white sunlight is a mixture of all the colors in the visible spectrum — and these travel at varying wavelengths — then each color will refract at a different angle when a beam of sunlight passes through a glass prism.

The Experiments : Color was a scientific mystery before Isaac Newton came along. During the summer of 1665, he started experimenting with glass prisms from the safety of a darkened room in Cambridge, England.

He cut a quarter-inch (0.63-centimeter) circular hole into one of the window shutters, allowing a single beam of sunlight to enter the place. When Newton held up a prism to this ray, an oblong patch of multicolored light was projected onto the opposite wall.

This contained segregated layers of red, orange, yellow, green, blue, indigo and violet light. From top to bottom, this patch measured 13.5 inches (33.65 centimeters) tall, yet it was only 2.6 inches (6.6 centimeters) across.

Newton deduced that these vibrant colors had been hiding within the sunlight itself, but the prism bent (or "refracted") them at different angles, which separated the colors out.

Still, he wasn't 100 percent sure. So Newton replicated the experiment with one small change. This time, he took a second prism and had it intercept the rainbow-like patch of light. Once the refracted colors entered the new prism, they recombined into a circular white sunbeam. In other words, Newton took a ray of white light, broke it apart into a bunch of different colors and then reassembled it. What a neat party trick!

The Verdict : Sunlight really is a blend of all the colors in the rainbow — and yes, these can be individually separated via light refraction.

The Hypothesis : If predators limit the populations of the organisms they attack, then we'd expect the prey species to become more common after the eradication of a major predator.

The Experiment : Meet Pisaster ochraceus , also known as the purple sea star (or the purple starfish if you prefer).

Using an extendable stomach , the creature feeds on mussels, limpets, barnacles, snails and other hapless victims. On some seaside rocks (and tidal pools) along the coast of Washington state, this starfish is the apex predator.

The animal made Robert Paine a scientific celebrity. An ecologist by trade, Paine was fascinated by the environmental roles of top predators. In June 1963, he kicked off an ambitious experiment along Washington state's Mukkaw Bay. For years on end, Paine kept a rocky section of this shoreline completely starfish-free.

It was hard work. Paine had to regularly pry wayward sea stars off "his" outcrop — sometimes with a crowbar. Then he'd chuck them into the ocean.

Before the experiment, Paine observed 15 different species of animals and algae inhabiting the area he decided to test. By June 1964 — one year after his starfish purge started — that number had dropped to eight .

Unchecked by purple sea stars, the barnacle population skyrocketed. Subsequently, these were replaced by California mussels , which came to dominate the terrain. By anchoring themselves to rocks in great numbers, the mussels edged out other life-forms. That made the outcrop uninhabitable to most former residents: Even sponges, anemones and algae — organisms that Pisaster ochraceus doesn't eat — were largely evicted.

All those species continued to thrive on another piece of shoreline that Paine left untouched. Later experiments convinced him that Pisaster ochraceus is a " keystone species ," a creature who exerts disproportionate influence over its environment. Eliminate the keystone and the whole system gets disheveled.

The Verdict : Apex predators don't just affect the animals that they hunt. Removing a top predator sets off a chain reaction that can fundamentally transform an entire ecosystem.

Contrary to popular belief, Pavlov almost never used bells in his dog experiments. Instead, he preferred metronomes, buzzers, harmoniums and electric shocks.

Frequently Asked Questions

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• Null and Alternative Hypotheses | Definitions & Examples

Null & Alternative Hypotheses | Definitions, Templates & Examples

Published on May 6, 2022 by Shaun Turney . Revised on June 22, 2023.

The null and alternative hypotheses are two competing claims that researchers weigh evidence for and against using a statistical test :

• Null hypothesis ( H 0 ): There’s no effect in the population .
• Alternative hypothesis ( H a or H 1 ) : There’s an effect in the population.

Table of contents

Answering your research question with hypotheses, what is a null hypothesis, what is an alternative hypothesis, similarities and differences between null and alternative hypotheses, how to write null and alternative hypotheses, other interesting articles, frequently asked questions.

The null and alternative hypotheses offer competing answers to your research question . When the research question asks “Does the independent variable affect the dependent variable?”:

• The null hypothesis ( H 0 ) answers “No, there’s no effect in the population.”
• The alternative hypothesis ( H a ) answers “Yes, there is an effect in the population.”

The null and alternative are always claims about the population. That’s because the goal of hypothesis testing is to make inferences about a population based on a sample . Often, we infer whether there’s an effect in the population by looking at differences between groups or relationships between variables in the sample. It’s critical for your research to write strong hypotheses .

You can use a statistical test to decide whether the evidence favors the null or alternative hypothesis. Each type of statistical test comes with a specific way of phrasing the null and alternative hypothesis. However, the hypotheses can also be phrased in a general way that applies to any test.

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The null hypothesis is the claim that there’s no effect in the population.

If the sample provides enough evidence against the claim that there’s no effect in the population ( p ≤ α), then we can reject the null hypothesis . Otherwise, we fail to reject the null hypothesis.

Although “fail to reject” may sound awkward, it’s the only wording that statisticians accept . Be careful not to say you “prove” or “accept” the null hypothesis.

Null hypotheses often include phrases such as “no effect,” “no difference,” or “no relationship.” When written in mathematical terms, they always include an equality (usually =, but sometimes ≥ or ≤).

You can never know with complete certainty whether there is an effect in the population. Some percentage of the time, your inference about the population will be incorrect. When you incorrectly reject the null hypothesis, it’s called a type I error . When you incorrectly fail to reject it, it’s a type II error.

Examples of null hypotheses

The table below gives examples of research questions and null hypotheses. There’s always more than one way to answer a research question, but these null hypotheses can help you get started.

*Note that some researchers prefer to always write the null hypothesis in terms of “no effect” and “=”. It would be fine to say that daily meditation has no effect on the incidence of depression and p 1 = p 2 .

The alternative hypothesis ( H a ) is the other answer to your research question . It claims that there’s an effect in the population.

Often, your alternative hypothesis is the same as your research hypothesis. In other words, it’s the claim that you expect or hope will be true.

The alternative hypothesis is the complement to the null hypothesis. Null and alternative hypotheses are exhaustive, meaning that together they cover every possible outcome. They are also mutually exclusive, meaning that only one can be true at a time.

Alternative hypotheses often include phrases such as “an effect,” “a difference,” or “a relationship.” When alternative hypotheses are written in mathematical terms, they always include an inequality (usually ≠, but sometimes < or >). As with null hypotheses, there are many acceptable ways to phrase an alternative hypothesis.

Examples of alternative hypotheses

The table below gives examples of research questions and alternative hypotheses to help you get started with formulating your own.

Null and alternative hypotheses are similar in some ways:

• They’re both answers to the research question.
• They both make claims about the population.
• They’re both evaluated by statistical tests.

However, there are important differences between the two types of hypotheses, summarized in the following table.

To help you write your hypotheses, you can use the template sentences below. If you know which statistical test you’re going to use, you can use the test-specific template sentences. Otherwise, you can use the general template sentences.

General template sentences

The only thing you need to know to use these general template sentences are your dependent and independent variables. To write your research question, null hypothesis, and alternative hypothesis, fill in the following sentences with your variables:

Does independent variable affect dependent variable ?

• Null hypothesis ( H 0 ): Independent variable does not affect dependent variable.
• Alternative hypothesis ( H a ): Independent variable affects dependent variable.

Test-specific template sentences

Once you know the statistical test you’ll be using, you can write your hypotheses in a more precise and mathematical way specific to the test you chose. The table below provides template sentences for common statistical tests.

Note: The template sentences above assume that you’re performing one-tailed tests . One-tailed tests are appropriate for most studies.

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

The null hypothesis is often abbreviated as H 0 . When the null hypothesis is written using mathematical symbols, it always includes an equality symbol (usually =, but sometimes ≥ or ≤).

The alternative hypothesis is often abbreviated as H a or H 1 . When the alternative hypothesis is written using mathematical symbols, it always includes an inequality symbol (usually ≠, but sometimes < or >).

A research hypothesis is your proposed answer to your research question. The research hypothesis usually includes an explanation (“ x affects y because …”).

A statistical hypothesis, on the other hand, is a mathematical statement about a population parameter. Statistical hypotheses always come in pairs: the null and alternative hypotheses . In a well-designed study , the statistical hypotheses correspond logically to the research hypothesis.

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• Scientific Methods

What is Hypothesis?

We have heard of many hypotheses which have led to great inventions in science. Assumptions that are made on the basis of some evidence are known as hypotheses. In this article, let us learn in detail about the hypothesis and the type of hypothesis with examples.

A hypothesis is an assumption that is made based on some evidence. This is the initial point of any investigation that translates the research questions into predictions. It includes components like variables, population and the relation between the variables. A research hypothesis is a hypothesis that is used to test the relationship between two or more variables.

Characteristics of Hypothesis

Following are the characteristics of the hypothesis:

• The hypothesis should be clear and precise to consider it to be reliable.
• If the hypothesis is a relational hypothesis, then it should be stating the relationship between variables.
• The hypothesis must be specific and should have scope for conducting more tests.
• The way of explanation of the hypothesis must be very simple and it should also be understood that the simplicity of the hypothesis is not related to its significance.

Sources of Hypothesis

Following are the sources of hypothesis:

• The resemblance between the phenomenon.
• Observations from past studies, present-day experiences and from the competitors.
• Scientific theories.
• General patterns that influence the thinking process of people.

Types of Hypothesis

There are six forms of hypothesis and they are:

• Simple hypothesis
• Complex hypothesis
• Directional hypothesis
• Non-directional hypothesis
• Null hypothesis
• Associative and casual hypothesis

Simple Hypothesis

It shows a relationship between one dependent variable and a single independent variable. For example – If you eat more vegetables, you will lose weight faster. Here, eating more vegetables is an independent variable, while losing weight is the dependent variable.

Complex Hypothesis

It shows the relationship between two or more dependent variables and two or more independent variables. Eating more vegetables and fruits leads to weight loss, glowing skin, and reduces the risk of many diseases such as heart disease.

Directional Hypothesis

It shows how a researcher is intellectual and committed to a particular outcome. The relationship between the variables can also predict its nature. For example- children aged four years eating proper food over a five-year period are having higher IQ levels than children not having a proper meal. This shows the effect and direction of the effect.

Non-directional Hypothesis

It is used when there is no theory involved. It is a statement that a relationship exists between two variables, without predicting the exact nature (direction) of the relationship.

Null Hypothesis

It provides a statement which is contrary to the hypothesis. It’s a negative statement, and there is no relationship between independent and dependent variables. The symbol is denoted by “H O ”.

Associative and Causal Hypothesis

Associative hypothesis occurs when there is a change in one variable resulting in a change in the other variable. Whereas, the causal hypothesis proposes a cause and effect interaction between two or more variables.

Examples of Hypothesis

Following are the examples of hypotheses based on their types:

• Consumption of sugary drinks every day leads to obesity is an example of a simple hypothesis.
• All lilies have the same number of petals is an example of a null hypothesis.
• If a person gets 7 hours of sleep, then he will feel less fatigue than if he sleeps less. It is an example of a directional hypothesis.

Functions of Hypothesis

Following are the functions performed by the hypothesis:

• Hypothesis helps in making an observation and experiments possible.
• It becomes the start point for the investigation.
• Hypothesis helps in verifying the observations.
• It helps in directing the inquiries in the right direction.

How will Hypothesis help in the Scientific Method?

Researchers use hypotheses to put down their thoughts directing how the experiment would take place. Following are the steps that are involved in the scientific method:

• Formation of question
• Doing background research
• Creation of hypothesis
• Designing an experiment
• Collection of data
• Result analysis
• Summarizing the experiment
• Communicating the results

Frequently Asked Questions – FAQs

What is hypothesis.

A hypothesis is an assumption made based on some evidence.

Give an example of simple hypothesis?

What are the types of hypothesis.

Types of hypothesis are:

• Associative and Casual hypothesis

State true or false: Hypothesis is the initial point of any investigation that translates the research questions into a prediction.

Define complex hypothesis..

A complex hypothesis shows the relationship between two or more dependent variables and two or more independent variables.

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From: Control of false discoveries in grouped hypothesis testing for eQTL data

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ExcelR Data Science Assignment No 3 Topics data-science statistics, seaborn ,matplotlib ,hypothesis-testing, statmodels ExcelR Data Science Assignment No 3

Hypothesis Testing : ---> Hypothesis testing is a part of statistics in which we make assumptions about the population parameter. So, hypothesis testing mentions a proper procedure by analysing a random sample of the population to accept or reject the assumption. Hypothesis testing is the way of trying to make sense of assumptions by looking at the sample data.

Type of Hypothesis : ---> The best way to determine whether a statistical hypothesis is true would be to examine the entire population. Since that is often impractical, researchers typically examine a random sample from the population. If sample data are not consistent with the statistical hypothesis, the hypothesis is rejected. There are two types of statistical hypotheses.

• Null Hypothesis : The null hypothesis, denoted by Ho, is usually the hypothesis that sample observations result purely from chance.

• Alternative Hypothesis : The alternative hypothesis, denoted by H1 or Ha, is the hypothesis that sample observations are influenced by some non-random cause.

This assignment will study following Questions: Question 1 A F&B manager wants to determine whether there is any significant difference in the diameter of the cutlet between two units. A randomly selected sample of cutlets was collected from both units and measured? Analyze the data and draw inferences at 5% significance level. Please state the assumptions and tests that you carried out to check validity of the assumptions.

Question 2 A F&B manager wants to determine whether there is any significant difference in the diameter of the cutlet between two units. A randomly selected sample of cutlets was collected from both units and measured? Analyze the data and draw inferences at 5% significance level. Please state the assumptions and tests that you carried out to check validity of the assumptions.

Question 3 Sales of products in four different regions is tabulated for males and females. Find if male-female buyer rations are similar across regions.

Question 4 TeleCall uses 4 centers around the globe to process customer order forms. They audit a certain % of the customer order forms. Any error in order form renders it defective and has to be reworked before processing. The manager wants to check whether the defective % varies by centre. Please analyze the data at 5% significance level and help the manager draw appropriate inferences

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