hypothesis in science experiment

4.14: Experiments and Hypotheses

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Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis, and it must be possible to prove your hypothesis true or false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

• No. This statement is not testable or falsifiable.
• No. This statement is not testable.
• No. This statement is not falsifiable.
• Yes. This statement is testable and falsifiable.

[reveal-answer q=”429550″] Show Answers [/reveal-answer] [hidden-answer a=”429550″]

• d: Yes. This statement is testable and falsifiable. This could be tested with a number of different kinds of observations and experiments, and it is possible to gather evidence that indicates that air pollution is not linked with asthma.
• a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

[/hidden-answer]

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

• Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
• There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
• Some types of HPV can cause cervical cancer.
• About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

• HPV causes cervical cancer.
• People should not have unprotected sex with many partners.
• People who get the vaccine will not get HPV.
• The HPV vaccine will protect people against cancer.

[reveal-answer q=”20917″] Show Answer [/reveal-answer] [hidden-answer a=”20917″]Hypothesis A is not the best choice because this information is already known from previous studies. Hypothesis B is not testable because scientific hypotheses are not value statements; they do not include judgments like “should,” “better than,” etc. Scientific evidence certainly might support this value judgment, but a hypothesis would take a different form: “Having unprotected sex with many partners increases a person’s risk for cervical cancer.” Before the researchers can test if the vaccine protects against cancer (hypothesis D), they want to test if it protects against the virus. This statement will make an excellent hypothesis for the next study. The researchers should first test hypothesis C—whether or not the new vaccine can prevent HPV.[/hidden-answer]

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—his or her views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

• This group is given a placebo.
• This group is deliberately infected with HPV.
• This group is given nothing.
• This group is given the HPV vaccine.

[reveal-answer q=”918962″] Show Answers [/reveal-answer] [hidden-answer a=”918962″]

• a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
• d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

• Sex (all of the subjects will be female)
• Presence or absence of the HPV vaccine
• Presence or absence of HPV (the virus)

[reveal-answer q=”68680″]Show Answer[/reveal-answer] [hidden-answer a=”68680″]Answer b. Presence or absence of the HPV vaccine. This is the variable that is different between the control and the experimental groups. All the subjects in this study are female, so this variable is the same in all groups. In a well-designed study, the two groups will be of similar age. The presence or absence of the virus is what the researchers will measure at the end of the experiment. Ideally the two groups will both be HPV-free at the start of the experiment.

List three control variables other than age.

[practice-area rows=”3″][/practice-area] [reveal-answer q=”903121″]Show Answer[/reveal-answer] [hidden-answer a=”903121″]Some possible control variables would be: general health of the women, sexual activity, lifestyle, diet, socioeconomic status, etc.

What is the dependent variable in this experiment?

• Sex (male or female)
• Rates of HPV infection
• Age (years)

[reveal-answer q=”907103″]Show Answer[/reveal-answer] [hidden-answer a=”907103″]Answer b. Rates of HPV infection. The researchers will measure how many individuals got infected with HPV after a given period of time.[/hidden-answer]

Contributors and Attributions

• Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
• Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

What is a scientific hypothesis?

It's the initial building block in the scientific method.

Hypothesis basics

What makes a hypothesis testable.

• Types of hypotheses
• Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

• If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
• If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
• If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

• Read more about writing a hypothesis, from the American Medical Writers Association.
• Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
• Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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Biology library

Course: biology library   >   unit 1.

• The scientific method
• Controlled experiments

The scientific method and experimental design

• (Choice A)   The facts collected from an experiment are written in the form of a hypothesis. A The facts collected from an experiment are written in the form of a hypothesis.
• (Choice B)   A hypothesis is the correct answer to a scientific question. B A hypothesis is the correct answer to a scientific question.
• (Choice C)   A hypothesis is a possible, testable explanation for a scientific question. C A hypothesis is a possible, testable explanation for a scientific question.
• (Choice D)   A hypothesis is the process of making careful observations. D A hypothesis is the process of making careful observations.

Scientific Hypothesis Examples

• Scientific Method
• Chemical Laws
• Periodic Table
• Projects & Experiments
• Biochemistry
• Physical Chemistry
• Medical Chemistry
• Chemistry In Everyday Life
• Famous Chemists
• Activities for Kids
• Abbreviations & Acronyms
• Weather & Climate
• Ph.D., Biomedical Sciences, University of Tennessee at Knoxville
• B.A., Physics and Mathematics, Hastings College

A hypothesis is an educated guess about what you think will happen in a scientific experiment, based on your observations. Before conducting the experiment, you propose a hypothesis so that you can determine if your prediction is supported.

There are several ways you can state a hypothesis, but the best hypotheses are ones you can test and easily refute. Why would you want to disprove or discard your own hypothesis? Well, it is the easiest way to demonstrate that two factors are related. Here are some good scientific hypothesis examples:

• Hypothesis: All forks have three tines. This would be disproven if you find any fork with a different number of tines.
• Hypothesis: There is no relationship between smoking and lung cancer. While it is difficult to establish cause and effect in health issues, you can apply statistics to data to discredit or support this hypothesis.
• Hypothesis: Plants require liquid water to survive. This would be disproven if you find a plant that doesn't need it.
• Hypothesis: Cats do not show a paw preference (equivalent to being right- or left-handed). You could gather data around the number of times cats bat at a toy with either paw and analyze the data to determine whether cats, on the whole, favor one paw over the other. Be careful here, because individual cats, like people, might (or might not) express a preference. A large sample size would be helpful.
• Hypothesis: If plants are watered with a 10% detergent solution, their growth will be negatively affected. Some people prefer to state a hypothesis in an "If, then" format. An alternate hypothesis might be: Plant growth will be unaffected by water with a 10% detergent solution.
• Scientific Hypothesis, Model, Theory, and Law
• What Are the Elements of a Good Hypothesis?
• What Is a Hypothesis? (Science)
• Understanding Simple vs Controlled Experiments
• Six Steps of the Scientific Method
• What Is a Testable Hypothesis?
• Null Hypothesis Definition and Examples
• What Are Examples of a Hypothesis?
• Theory Definition in Science
• How To Design a Science Fair Experiment
• Science Projects for Every Subject
• Null Hypothesis Examples
• What 'Fail to Reject' Means in a Hypothesis Test
• Middle School Science Fair Project Ideas
• Effect of Acids and Bases on the Browning of Apples

Scientific Method: Step 3: HYPOTHESIS

• Step 1: QUESTION
• Step 2: RESEARCH
• Step 3: HYPOTHESIS
• Step 4: EXPERIMENT
• Step 5: DATA
• Step 6: CONCLUSION

Step 3: State your hypothesis

Now it's time to state your hypothesis . The hypothesis is an educated guess as to what will happen during your experiment. 

The hypothesis is often written using the words "IF" and "THEN." For example, " If I do not study, then I will fail the test." The "if' and "then" statements reflect your independent and dependent variables . 

The hypothesis should relate back to your original question and must be testable .

A word about variables...

Your experiment will include variables to measure and to explain any cause and effect. Below you will find some useful links describing the different types of variables.

• "What are independent and dependent variables" NCES
• [VIDEO] Biology: Independent vs. Dependent Variables (Nucleus Medical Media) Video explaining independent and dependent variables, with examples.

Resource Links

• What is and How to Write a Good Hypothesis in Research? (Elsevier)
• Hypothesis brochure from Penn State/Berks

• << Previous: Step 2: RESEARCH
• Next: Step 4: EXPERIMENT >>
• Last Updated: Jan 26, 2024 10:39 AM
• URL: https://harford.libguides.com/scientific_method

Writing an Introduction for a Scientific Paper

Dr. michelle harris, dr. janet batzli, biocore.

This section provides guidelines on how to construct a solid introduction to a scientific paper including background information, study question , biological rationale, hypothesis , and general approach . If the Introduction is done well, there should be no question in the reader’s mind why and on what basis you have posed a specific hypothesis.

Broad Question : based on an initial observation (e.g., “I see a lot of guppies close to the shore. Do guppies like living in shallow water?”). This observation of the natural world may inspire you to investigate background literature or your observation could be based on previous research by others or your own pilot study. Broad questions are not always included in your written text, but are essential for establishing the direction of your research.

Background Information : key issues, concepts, terminology, and definitions needed to understand the biological rationale for the experiment. It often includes a summary of findings from previous, relevant studies. Remember to cite references, be concise, and only include relevant information given your audience and your experimental design. Concisely summarized background information leads to the identification of specific scientific knowledge gaps that still exist. (e.g., “No studies to date have examined whether guppies do indeed spend more time in shallow water.”)

Testable Question : these questions are much more focused than the initial broad question, are specific to the knowledge gap identified, and can be addressed with data. (e.g., “Do guppies spend different amounts of time in water <1 meter deep as compared to their time in water that is >1 meter deep?”)

Biological Rationale : describes the purpose of your experiment distilling what is known and what is not known that defines the knowledge gap that you are addressing. The “BR” provides the logic for your hypothesis and experimental approach, describing the biological mechanism and assumptions that explain why your hypothesis should be true.

The biological rationale is based on your interpretation of the scientific literature, your personal observations, and the underlying assumptions you are making about how you think the system works. If you have written your biological rationale, your reader should see your hypothesis in your introduction section and say to themselves, “Of course, this hypothesis seems very logical based on the rationale presented.”

• A thorough rationale defines your assumptions about the system that have not been revealed in scientific literature or from previous systematic observation. These assumptions drive the direction of your specific hypothesis or general predictions.
• Defining the rationale is probably the most critical task for a writer, as it tells your reader why your research is biologically meaningful. It may help to think about the rationale as an answer to the questions— how is this investigation related to what we know, what assumptions am I making about what we don’t yet know, AND how will this experiment add to our knowledge? *There may or may not be broader implications for your study; be careful not to overstate these (see note on social justifications below).
• Expect to spend time and mental effort on this. You may have to do considerable digging into the scientific literature to define how your experiment fits into what is already known and why it is relevant to pursue.
• Be open to the possibility that as you work with and think about your data, you may develop a deeper, more accurate understanding of the experimental system. You may find the original rationale needs to be revised to reflect your new, more sophisticated understanding.
• As you progress through Biocore and upper level biology courses, your rationale should become more focused and matched with the level of study e ., cellular, biochemical, or physiological mechanisms that underlie the rationale. Achieving this type of understanding takes effort, but it will lead to better communication of your science.

***Special note on avoiding social justifications: You should not overemphasize the relevance of your experiment and the possible connections to large-scale processes. Be realistic and logical —do not overgeneralize or state grand implications that are not sensible given the structure of your experimental system. Not all science is easily applied to improving the human condition. Performing an investigation just for the sake of adding to our scientific knowledge (“pure or basic science”) is just as important as applied science. In fact, basic science often provides the foundation for applied studies.

Hypothesis / Predictions : specific prediction(s) that you will test during your experiment. For manipulative experiments, the hypothesis should include the independent variable (what you manipulate), the dependent variable(s) (what you measure), the organism or system , the direction of your results, and comparison to be made.

If you are doing a systematic observation , your hypothesis presents a variable or set of variables that you predict are important for helping you characterize the system as a whole, or predict differences between components/areas of the system that help you explain how the system functions or changes over time.

Experimental Approach : Briefly gives the reader a general sense of the experiment, the type of data it will yield, and the kind of conclusions you expect to obtain from the data. Do not confuse the experimental approach with the experimental protocol . The experimental protocol consists of the detailed step-by-step procedures and techniques used during the experiment that are to be reported in the Methods and Materials section.

Some Final Tips on Writing an Introduction

• As you progress through the Biocore sequence, for instance, from organismal level of Biocore 301/302 to the cellular level in Biocore 303/304, we expect the contents of your “Introduction” paragraphs to reflect the level of your coursework and previous writing experience. For example, in Biocore 304 (Cell Biology Lab) biological rationale should draw upon assumptions we are making about cellular and biochemical processes.
• Be Concise yet Specific: Remember to be concise and only include relevant information given your audience and your experimental design. As you write, keep asking, “Is this necessary information or is this irrelevant detail?” For example, if you are writing a paper claiming that a certain compound is a competitive inhibitor to the enzyme alkaline phosphatase and acts by binding to the active site, you need to explain (briefly) Michaelis-Menton kinetics and the meaning and significance of Km and Vmax. This explanation is not necessary if you are reporting the dependence of enzyme activity on pH because you do not need to measure Km and Vmax to get an estimate of enzyme activity.
• Another example: if you are writing a paper reporting an increase in Daphnia magna heart rate upon exposure to caffeine you need not describe the reproductive cycle of magna unless it is germane to your results and discussion. Be specific and concrete, especially when making introductory or summary statements.

Where Do You Discuss Pilot Studies? Many times it is important to do pilot studies to help you get familiar with your experimental system or to improve your experimental design. If your pilot study influences your biological rationale or hypothesis, you need to describe it in your Introduction. If your pilot study simply informs the logistics or techniques, but does not influence your rationale, then the description of your pilot study belongs in the Materials and Methods section.  

How will introductions be evaluated? The following is part of the rubric we will be using to evaluate your papers.

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The Craft of Writing a Strong Hypothesis

Table of Contents

Writing a hypothesis is one of the essential elements of a scientific research paper. It needs to be to the point, clearly communicating what your research is trying to accomplish. A blurry, drawn-out, or complexly-structured hypothesis can confuse your readers. Or worse, the editor and peer reviewers.

A captivating hypothesis is not too intricate. This blog will take you through the process so that, by the end of it, you have a better idea of how to convey your research paper's intent in just one sentence.

What is a Hypothesis?

The first step in your scientific endeavor, a hypothesis, is a strong, concise statement that forms the basis of your research. It is not the same as a thesis statement , which is a brief summary of your research paper .

The sole purpose of a hypothesis is to predict your paper's findings, data, and conclusion. It comes from a place of curiosity and intuition . When you write a hypothesis, you're essentially making an educated guess based on scientific prejudices and evidence, which is further proven or disproven through the scientific method.

The reason for undertaking research is to observe a specific phenomenon. A hypothesis, therefore, lays out what the said phenomenon is. And it does so through two variables, an independent and dependent variable.

The independent variable is the cause behind the observation, while the dependent variable is the effect of the cause. A good example of this is “mixing red and blue forms purple.” In this hypothesis, mixing red and blue is the independent variable as you're combining the two colors at your own will. The formation of purple is the dependent variable as, in this case, it is conditional to the independent variable.

Different Types of Hypotheses‌

Types of hypotheses

Some would stand by the notion that there are only two types of hypotheses: a Null hypothesis and an Alternative hypothesis. While that may have some truth to it, it would be better to fully distinguish the most common forms as these terms come up so often, which might leave you out of context.

Apart from Null and Alternative, there are Complex, Simple, Directional, Non-Directional, Statistical, and Associative and casual hypotheses. They don't necessarily have to be exclusive, as one hypothesis can tick many boxes, but knowing the distinctions between them will make it easier for you to construct your own.

1. Null hypothesis

A null hypothesis proposes no relationship between two variables. Denoted by H 0 , it is a negative statement like “Attending physiotherapy sessions does not affect athletes' on-field performance.” Here, the author claims physiotherapy sessions have no effect on on-field performances. Even if there is, it's only a coincidence.

2. Alternative hypothesis

Considered to be the opposite of a null hypothesis, an alternative hypothesis is donated as H1 or Ha. It explicitly states that the dependent variable affects the independent variable. A good  alternative hypothesis example is “Attending physiotherapy sessions improves athletes' on-field performance.” or “Water evaporates at 100 °C. ” The alternative hypothesis further branches into directional and non-directional.

• Directional hypothesis: A hypothesis that states the result would be either positive or negative is called directional hypothesis. It accompanies H1 with either the ‘<' or ‘>' sign.
• Non-directional hypothesis: A non-directional hypothesis only claims an effect on the dependent variable. It does not clarify whether the result would be positive or negative. The sign for a non-directional hypothesis is ‘≠.'

3. Simple hypothesis

A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, “Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking.

4. Complex hypothesis

In contrast to a simple hypothesis, a complex hypothesis implies the relationship between multiple independent and dependent variables. For instance, “Individuals who eat more fruits tend to have higher immunity, lesser cholesterol, and high metabolism.” The independent variable is eating more fruits, while the dependent variables are higher immunity, lesser cholesterol, and high metabolism.

5. Associative and casual hypothesis

Associative and casual hypotheses don't exhibit how many variables there will be. They define the relationship between the variables. In an associative hypothesis, changing any one variable, dependent or independent, affects others. In a casual hypothesis, the independent variable directly affects the dependent.

6. Empirical hypothesis

Also referred to as the working hypothesis, an empirical hypothesis claims a theory's validation via experiments and observation. This way, the statement appears justifiable and different from a wild guess.

Say, the hypothesis is “Women who take iron tablets face a lesser risk of anemia than those who take vitamin B12.” This is an example of an empirical hypothesis where the researcher  the statement after assessing a group of women who take iron tablets and charting the findings.

7. Statistical hypothesis

The point of a statistical hypothesis is to test an already existing hypothesis by studying a population sample. Hypothesis like “44% of the Indian population belong in the age group of 22-27.” leverage evidence to prove or disprove a particular statement.

Characteristics of a Good Hypothesis

Writing a hypothesis is essential as it can make or break your research for you. That includes your chances of getting published in a journal. So when you're designing one, keep an eye out for these pointers:

• A research hypothesis has to be simple yet clear to look justifiable enough.
• It has to be testable — your research would be rendered pointless if too far-fetched into reality or limited by technology.
• It has to be precise about the results —what you are trying to do and achieve through it should come out in your hypothesis.
• A research hypothesis should be self-explanatory, leaving no doubt in the reader's mind.
• If you are developing a relational hypothesis, you need to include the variables and establish an appropriate relationship among them.
• A hypothesis must keep and reflect the scope for further investigations and experiments.

Separating a Hypothesis from a Prediction

Outside of academia, hypothesis and prediction are often used interchangeably. In research writing, this is not only confusing but also incorrect. And although a hypothesis and prediction are guesses at their core, there are many differences between them.

A hypothesis is an educated guess or even a testable prediction validated through research. It aims to analyze the gathered evidence and facts to define a relationship between variables and put forth a logical explanation behind the nature of events.

Predictions are assumptions or expected outcomes made without any backing evidence. They are more fictionally inclined regardless of where they originate from.

For this reason, a hypothesis holds much more weight than a prediction. It sticks to the scientific method rather than pure guesswork. "Planets revolve around the Sun." is an example of a hypothesis as it is previous knowledge and observed trends. Additionally, we can test it through the scientific method.

Whereas "COVID-19 will be eradicated by 2030." is a prediction. Even though it results from past trends, we can't prove or disprove it. So, the only way this gets validated is to wait and watch if COVID-19 cases end by 2030.

Finally, How to Write a Hypothesis

Quick tips on writing a hypothesis

1.  Be clear about your research question

A hypothesis should instantly address the research question or the problem statement. To do so, you need to ask a question. Understand the constraints of your undertaken research topic and then formulate a simple and topic-centric problem. Only after that can you develop a hypothesis and further test for evidence.

2. Carry out a recce

Once you have your research's foundation laid out, it would be best to conduct preliminary research. Go through previous theories, academic papers, data, and experiments before you start curating your research hypothesis. It will give you an idea of your hypothesis's viability or originality.

Making use of references from relevant research papers helps draft a good research hypothesis. SciSpace Discover offers a repository of over 270 million research papers to browse through and gain a deeper understanding of related studies on a particular topic. Additionally, you can use SciSpace Copilot , your AI research assistant, for reading any lengthy research paper and getting a more summarized context of it. A hypothesis can be formed after evaluating many such summarized research papers. Copilot also offers explanations for theories and equations, explains paper in simplified version, allows you to highlight any text in the paper or clip math equations and tables and provides a deeper, clear understanding of what is being said. This can improve the hypothesis by helping you identify potential research gaps.

3. Create a 3-dimensional hypothesis

Variables are an essential part of any reasonable hypothesis. So, identify your independent and dependent variable(s) and form a correlation between them. The ideal way to do this is to write the hypothetical assumption in the ‘if-then' form. If you use this form, make sure that you state the predefined relationship between the variables.

In another way, you can choose to present your hypothesis as a comparison between two variables. Here, you must specify the difference you expect to observe in the results.

4. Write the first draft

Now that everything is in place, it's time to write your hypothesis. For starters, create the first draft. In this version, write what you expect to find from your research.

Clearly separate your independent and dependent variables and the link between them. Don't fixate on syntax at this stage. The goal is to ensure your hypothesis addresses the issue.

5. Proof your hypothesis

After preparing the first draft of your hypothesis, you need to inspect it thoroughly. It should tick all the boxes, like being concise, straightforward, relevant, and accurate. Your final hypothesis has to be well-structured as well.

Research projects are an exciting and crucial part of being a scholar. And once you have your research question, you need a great hypothesis to begin conducting research. Thus, knowing how to write a hypothesis is very important.

Now that you have a firmer grasp on what a good hypothesis constitutes, the different kinds there are, and what process to follow, you will find it much easier to write your hypothesis, which ultimately helps your research.

Now it's easier than ever to streamline your research workflow with SciSpace Discover . Its integrated, comprehensive end-to-end platform for research allows scholars to easily discover, write and publish their research and fosters collaboration.

It includes everything you need, including a repository of over 270 million research papers across disciplines, SEO-optimized summaries and public profiles to show your expertise and experience.

If you found these tips on writing a research hypothesis useful, head over to our blog on Statistical Hypothesis Testing to learn about the top researchers, papers, and institutions in this domain.

Frequently Asked Questions (FAQs)

1. what is the definition of hypothesis.

According to the Oxford dictionary, a hypothesis is defined as “An idea or explanation of something that is based on a few known facts, but that has not yet been proved to be true or correct”.

2. What is an example of hypothesis?

The hypothesis is a statement that proposes a relationship between two or more variables. An example: "If we increase the number of new users who join our platform by 25%, then we will see an increase in revenue."

3. What is an example of null hypothesis?

A null hypothesis is a statement that there is no relationship between two variables. The null hypothesis is written as H0. The null hypothesis states that there is no effect. For example, if you're studying whether or not a particular type of exercise increases strength, your null hypothesis will be "there is no difference in strength between people who exercise and people who don't."

4. What are the types of research?

• Fundamental research

• Applied research

• Qualitative research

• Quantitative research

• Mixed research

• Exploratory research

• Longitudinal research

• Cross-sectional research

• Field research

• Laboratory research

• Fixed research

• Flexible research

• Action research

• Policy research

• Classification research

• Comparative research

• Causal research

• Inductive research

• Deductive research

5. How to write a hypothesis?

• Your hypothesis should be able to predict the relationship and outcome.

• Avoid wordiness by keeping it simple and brief.

• Your hypothesis should contain observable and testable outcomes.

• Your hypothesis should be relevant to the research question.

6. What are the 2 types of hypothesis?

• Null hypotheses are used to test the claim that "there is no difference between two groups of data".

• Alternative hypotheses test the claim that "there is a difference between two data groups".

7. Difference between research question and research hypothesis?

A research question is a broad, open-ended question you will try to answer through your research. A hypothesis is a statement based on prior research or theory that you expect to be true due to your study. Example - Research question: What are the factors that influence the adoption of the new technology? Research hypothesis: There is a positive relationship between age, education and income level with the adoption of the new technology.

8. What is plural for hypothesis?

The plural of hypothesis is hypotheses. Here's an example of how it would be used in a statement, "Numerous well-considered hypotheses are presented in this part, and they are supported by tables and figures that are well-illustrated."

9. What is the red queen hypothesis?

The red queen hypothesis in evolutionary biology states that species must constantly evolve to avoid extinction because if they don't, they will be outcompeted by other species that are evolving. Leigh Van Valen first proposed it in 1973; since then, it has been tested and substantiated many times.

10. Who is known as the father of null hypothesis?

The father of the null hypothesis is Sir Ronald Fisher. He published a paper in 1925 that introduced the concept of null hypothesis testing, and he was also the first to use the term itself.

11. When to reject null hypothesis?

You need to find a significant difference between your two populations to reject the null hypothesis. You can determine that by running statistical tests such as an independent sample t-test or a dependent sample t-test. You should reject the null hypothesis if the p-value is less than 0.05.

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Experiment Definition in Science – What Is a Science Experiment?

In science, an experiment is simply a test of a hypothesis in the scientific method . It is a controlled examination of cause and effect. Here is a look at what a science experiment is (and is not), the key factors in an experiment, examples, and types of experiments.

Experiment Definition in Science

By definition, an experiment is a procedure that tests a hypothesis. A hypothesis, in turn, is a prediction of cause and effect or the predicted outcome of changing one factor of a situation. Both the hypothesis and experiment are components of the scientific method. The steps of the scientific method are:

• Make observations.
• Ask a question or identify a problem.
• State a hypothesis.
• Perform an experiment that tests the hypothesis.
• Based on the results of the experiment, either accept or reject the hypothesis.
• Draw conclusions and report the outcome of the experiment.

Key Parts of an Experiment

The two key parts of an experiment are the independent and dependent variables. The independent variable is the one factor that you control or change in an experiment. The dependent variable is the factor that you measure that responds to the independent variable. An experiment often includes other types of variables , but at its heart, it’s all about the relationship between the independent and dependent variable.

Examples of Experiments

Fertilizer and plant size.

For example, you think a certain fertilizer helps plants grow better. You’ve watched your plants grow and they seem to do better when they have the fertilizer compared to when they don’t. But, observations are only the beginning of science. So, you state a hypothesis: Adding fertilizer increases plant size. Note, you could have stated the hypothesis in different ways. Maybe you think the fertilizer increases plant mass or fruit production, for example. However you state the hypothesis, it includes both the independent and dependent variables. In this case, the independent variable is the presence or absence of fertilizer. The dependent variable is the response to the independent variable, which is the size of the plants.

Now that you have a hypothesis, the next step is designing an experiment that tests it. Experimental design is very important because the way you conduct an experiment influences its outcome. For example, if you use too small of an amount of fertilizer you may see no effect from the treatment. Or, if you dump an entire container of fertilizer on a plant you could kill it! So, recording the steps of the experiment help you judge the outcome of the experiment and aid others who come after you and examine your work. Other factors that might influence your results might include the species of plant and duration of the treatment. Record any conditions that might affect the outcome. Ideally, you want the only difference between your two groups of plants to be whether or not they receive fertilizer. Then, measure the height of the plants and see if there is a difference between the two groups.

Salt and Cookies

You don’t need a lab for an experiment. For example, consider a baking experiment. Let’s say you like the flavor of salt in your cookies, but you’re pretty sure the batch you made using extra salt fell a bit flat. If you double the amount of salt in a recipe, will it affect their size? Here, the independent variable is the amount of salt in the recipe and the dependent variable is cookie size.

Test this hypothesis with an experiment. Bake cookies using the normal recipe (your control group ) and bake some using twice the salt (the experimental group). Make sure it’s the exact same recipe. Bake the cookies at the same temperature and for the same time. Only change the amount of salt in the recipe. Then measure the height or diameter of the cookies and decide whether to accept or reject the hypothesis.

Examples of Things That Are Not Experiments

Based on the examples of experiments, you should see what is not an experiment:

• Making observations does not constitute an experiment. Initial observations often lead to an experiment, but are not a substitute for one.
• Making a model is not an experiment.
• Neither is making a poster.
• Just trying something to see what happens is not an experiment. You need a hypothesis or prediction about the outcome.
• Changing a lot of things at once isn’t an experiment. You only have one independent and one dependent variable. However, in an experiment, you might suspect the independent variable has an effect on a separate. So, you design a new experiment to test this.

Types of Experiments

There are three main types of experiments: controlled experiments, natural experiments, and field experiments,

• Controlled experiment : A controlled experiment compares two groups of samples that differ only in independent variable. For example, a drug trial compares the effect of a group taking a placebo (control group) against those getting the drug (the treatment group). Experiments in a lab or home generally are controlled experiments
• Natural experiment : Another name for a natural experiment is a quasi-experiment. In this type of experiment, the researcher does not directly control the independent variable, plus there may be other variables at play. Here, the goal is establishing a correlation between the independent and dependent variable. For example, in the formation of new elements a scientist hypothesizes that a certain collision between particles creates a new atom. But, other outcomes may be possible. Or, perhaps only decay products are observed that indicate the element, and not the new atom itself. Many fields of science rely on natural experiments, since controlled experiments aren’t always possible.
• Field experiment : While a controlled experiments takes place in a lab or other controlled setting, a field experiment occurs in a natural setting. Some phenomena cannot be readily studied in a lab or else the setting exerts an influence that affects the results. So, a field experiment may have higher validity. However, since the setting is not controlled, it is also subject to external factors and potential contamination. For example, if you study whether a certain plumage color affects bird mate selection, a field experiment in a natural environment eliminates the stressors of an artificial environment. Yet, other factors that could be controlled in a lab may influence results. For example, nutrition and health are controlled in a lab, but not in the field.
• Bailey, R.A. (2008). Design of Comparative Experiments . Cambridge: Cambridge University Press. ISBN 9780521683579.
• di Francia, G. Toraldo (1981). The Investigation of the Physical World . Cambridge University Press. ISBN 0-521-29925-X.
• Hinkelmann, Klaus; Kempthorne, Oscar (2008). Design and Analysis of Experiments. Volume I: Introduction to Experimental Design (2nd ed.). Wiley. ISBN 978-0-471-72756-9.
• Holland, Paul W. (December 1986). “Statistics and Causal Inference”.  Journal of the American Statistical Association . 81 (396): 945–960. doi: 10.2307/2289064
• Stohr-Hunt, Patricia (1996). “An Analysis of Frequency of Hands-on Experience and Science Achievement”. Journal of Research in Science Teaching . 33 (1): 101–109. doi: 10.1002/(SICI)1098-2736(199601)33:1<101::AID-TEA6>3.0.CO;2-Z

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How to Write a Hypothesis? Types and Examples 

All research studies involve the use of the scientific method, which is a mathematical and experimental technique used to conduct experiments by developing and testing a hypothesis or a prediction about an outcome. Simply put, a hypothesis is a suggested solution to a problem. It includes elements that are expressed in terms of relationships with each other to explain a condition or an assumption that hasn’t been verified using facts. 1 The typical steps in a scientific method include developing such a hypothesis, testing it through various methods, and then modifying it based on the outcomes of the experiments.  

A research hypothesis can be defined as a specific, testable prediction about the anticipated results of a study. 2 Hypotheses help guide the research process and supplement the aim of the study. After several rounds of testing, hypotheses can help develop scientific theories. 3 Hypotheses are often written as if-then statements. 

Here are two hypothesis examples: 

Dandelions growing in nitrogen-rich soils for two weeks develop larger leaves than those in nitrogen-poor soils because nitrogen stimulates vegetative growth. 4  

If a company offers flexible work hours, then their employees will be happier at work. 5  

Table of Contents

• What is a hypothesis? 
• Types of hypotheses 
• Characteristics of a hypothesis 
• Functions of a hypothesis 
• How to write a hypothesis 
• Hypothesis examples 
• Frequently asked questions 

What is a hypothesis?

A hypothesis expresses an expected relationship between variables in a study and is developed before conducting any research. Hypotheses are not opinions but rather are expected relationships based on facts and observations. They help support scientific research and expand existing knowledge. An incorrectly formulated hypothesis can affect the entire experiment leading to errors in the results so it’s important to know how to formulate a hypothesis and develop it carefully.

A few sources of a hypothesis include observations from prior studies, current research and experiences, competitors, scientific theories, and general conditions that can influence people. Figure 1 depicts the different steps in a research design and shows where exactly in the process a hypothesis is developed. 4  

There are seven different types of hypotheses—simple, complex, directional, nondirectional, associative and causal, null, and alternative. 

Types of hypotheses

The seven types of hypotheses are listed below: 5 , 6,7  

• Simple : Predicts the relationship between a single dependent variable and a single independent variable. 

Example: Exercising in the morning every day will increase your productivity.  

• Complex : Predicts the relationship between two or more variables. 

Example: Spending three hours or more on social media daily will negatively affect children’s mental health and productivity, more than that of adults.  

• Directional : Specifies the expected direction to be followed and uses terms like increase, decrease, positive, negative, more, or less. 

Example: The inclusion of intervention X decreases infant mortality compared to the original treatment.  

• Non-directional : Does not predict the exact direction, nature, or magnitude of the relationship between two variables but rather states the existence of a relationship. This hypothesis may be used when there is no underlying theory or if findings contradict prior research. 

Example: Cats and dogs differ in the amount of affection they express.  

• Associative and causal : An associative hypothesis suggests an interdependency between variables, that is, how a change in one variable changes the other.  

Example: There is a positive association between physical activity levels and overall health.  

A causal hypothesis, on the other hand, expresses a cause-and-effect association between variables. 

Example: Long-term alcohol use causes liver damage.  

• Null : Claims that the original hypothesis is false by showing that there is no relationship between the variables. 

Example: Sleep duration does not have any effect on productivity.  

• Alternative : States the opposite of the null hypothesis, that is, a relationship exists between two variables. 

Example: Sleep duration affects productivity.  

Characteristics of a hypothesis

So, what makes a good hypothesis? Here are some important characteristics of a hypothesis. 8,9  

• Testable : You must be able to test the hypothesis using scientific methods to either accept or reject the prediction. 
• Falsifiable : It should be possible to collect data that reject rather than support the hypothesis. 
• Logical : Hypotheses shouldn’t be a random guess but rather should be based on previous theories, observations, prior research, and logical reasoning. 
• Positive : The hypothesis statement about the existence of an association should be positive, that is, it should not suggest that an association does not exist. Therefore, the language used and knowing how to phrase a hypothesis is very important. 
• Clear and accurate : The language used should be easily comprehensible and use correct terminology. 
• Relevant : The hypothesis should be relevant and specific to the research question. 
• Structure : Should include all the elements that make a good hypothesis: variables, relationship, and outcome. 

Functions of a hypothesis

The following list mentions some important functions of a hypothesis: 1  

• Maintains the direction and progress of the research. 
• Expresses the important assumptions underlying the proposition in a single statement. 
• Establishes a suitable context for researchers to begin their investigation and for readers who are referring to the final report. 
• Provides an explanation for the occurrence of a specific phenomenon. 
• Ensures selection of appropriate and accurate facts necessary and relevant to the research subject. 

To summarize, a hypothesis provides the conceptual elements that complete the known data, conceptual relationships that systematize unordered elements, and conceptual meanings and interpretations that explain the unknown phenomena. 1  

How to write a hypothesis

Listed below are the main steps explaining how to write a hypothesis. 2,4,5  

• Make an observation and identify variables : Observe the subject in question and try to recognize a pattern or a relationship between the variables involved. This step provides essential background information to begin your research.  

For example, if you notice that an office’s vending machine frequently runs out of a specific snack, you may predict that more people in the office choose that snack over another. 

• Identify the main research question : After identifying a subject and recognizing a pattern, the next step is to ask a question that your hypothesis will answer.  

For example, after observing employees’ break times at work, you could ask “why do more employees take breaks in the morning rather than in the afternoon?” 

• Conduct some preliminary research to ensure originality and novelty : Your initial answer, which is your hypothesis, to the question is based on some pre-existing information about the subject. However, to ensure that your hypothesis has not been asked before or that it has been asked but rejected by other researchers you would need to gather additional information.  

For example, based on your observations you might state a hypothesis that employees work more efficiently when the air conditioning in the office is set at a lower temperature. However, during your preliminary research you find that this hypothesis was proven incorrect by a prior study. 

• Develop a general statement : After your preliminary research has confirmed the originality of your proposed answer, draft a general statement that includes all variables, subjects, and predicted outcome. The statement could be if/then or declarative.  
• Finalize the hypothesis statement : Use the PICOT model, which clarifies how to word a hypothesis effectively, when finalizing the statement. This model lists the important components required to write a hypothesis. 

P opulation: The specific group or individual who is the main subject of the research 

I nterest: The main concern of the study/research question 

C omparison: The main alternative group 

O utcome: The expected results  

T ime: Duration of the experiment 

Once you’ve finalized your hypothesis statement you would need to conduct experiments to test whether the hypothesis is true or false. 

Hypothesis examples

The following table provides examples of different types of hypotheses. 10 ,11  

Key takeaways  

Here’s a summary of all the key points discussed in this article about how to write a hypothesis. 

• A hypothesis is an assumption about an association between variables made based on limited evidence, which should be tested. 
• A hypothesis has four parts—the research question, independent variable, dependent variable, and the proposed relationship between the variables.   
• The statement should be clear, concise, testable, logical, and falsifiable. 
• There are seven types of hypotheses—simple, complex, directional, non-directional, associative and causal, null, and alternative. 
• A hypothesis provides a focus and direction for the research to progress. 
• A hypothesis plays an important role in the scientific method by helping to create an appropriate experimental design. 

Frequently asked questions

Hypotheses and research questions have different objectives and structure. The following table lists some major differences between the two. 9  

Here are a few examples to differentiate between a research question and hypothesis. 

Yes, here’s a simple checklist to help you gauge the effectiveness of your hypothesis. 9   1. When writing a hypothesis statement, check if it:  2. Predicts the relationship between the stated variables and the expected outcome.  3. Uses simple and concise language and is not wordy.  4. Does not assume readers’ knowledge about the subject.  5. Has observable, falsifiable, and testable results. 

As mentioned earlier in this article, a hypothesis is an assumption or prediction about an association between variables based on observations and simple evidence. These statements are usually generic. Research objectives, on the other hand, are more specific and dictated by hypotheses. The same hypothesis can be tested using different methods and the research objectives could be different in each case.     For example, Louis Pasteur observed that food lasts longer at higher altitudes, reasoned that it could be because the air at higher altitudes is cleaner (with fewer or no germs), and tested the hypothesis by exposing food to air cleaned in the laboratory. 12 Thus, a hypothesis is predictive—if the reasoning is correct, X will lead to Y—and research objectives are developed to test these predictions. 

Null hypothesis testing is a method to decide between two assumptions or predictions between variables (null and alternative hypotheses) in a statistical relationship in a sample. The null hypothesis, denoted as H 0 , claims that no relationship exists between variables in a population and any relationship in the sample reflects a sampling error or occurrence by chance. The alternative hypothesis, denoted as H 1 , claims that there is a relationship in the population. In every study, researchers need to decide whether the relationship in a sample occurred by chance or reflects a relationship in the population. This is done by hypothesis testing using the following steps: 13   1. Assume that the null hypothesis is true.  2. Determine how likely the sample relationship would be if the null hypothesis were true. This probability is called the p value.  3. If the sample relationship would be extremely unlikely, reject the null hypothesis and accept the alternative hypothesis. If the relationship would not be unlikely, accept the null hypothesis. 

To summarize, researchers should know how to write a good hypothesis to ensure that their research progresses in the required direction. A hypothesis is a testable prediction about any behavior or relationship between variables, usually based on facts and observation, and states an expected outcome.  

We hope this article has provided you with essential insight into the different types of hypotheses and their functions so that you can use them appropriately in your next research project. 

References  

• Dalen, DVV. The function of hypotheses in research. Proquest website. Accessed April 8, 2024. https://www.proquest.com/docview/1437933010?pq-origsite=gscholar&fromopenview=true&sourcetype=Scholarly%20Journals&imgSeq=1  
• McLeod S. Research hypothesis in psychology: Types & examples. SimplyPsychology website. Updated December 13, 2023. Accessed April 9, 2024. https://www.simplypsychology.org/what-is-a-hypotheses.html  
• Scientific method. Britannica website. Updated March 14, 2024. Accessed April 9, 2024. https://www.britannica.com/science/scientific-method  
• The hypothesis in science writing. Accessed April 10, 2024. https://berks.psu.edu/sites/berks/files/campus/HypothesisHandout_Final.pdf  
• How to develop a hypothesis (with elements, types, and examples). Indeed.com website. Updated February 3, 2023. Accessed April 10, 2024. https://www.indeed.com/career-advice/career-development/how-to-write-a-hypothesis  
• Types of research hypotheses. Excelsior online writing lab. Accessed April 11, 2024. https://owl.excelsior.edu/research/research-hypotheses/types-of-research-hypotheses/  
• What is a research hypothesis: how to write it, types, and examples. Researcher.life website. Published February 8, 2023. Accessed April 11, 2024. https://researcher.life/blog/article/how-to-write-a-research-hypothesis-definition-types-examples/  
• Developing a hypothesis. Pressbooks website. Accessed April 12, 2024. https://opentext.wsu.edu/carriecuttler/chapter/developing-a-hypothesis/  
• What is and how to write a good hypothesis in research. Elsevier author services website. Accessed April 12, 2024. https://scientific-publishing.webshop.elsevier.com/manuscript-preparation/what-how-write-good-hypothesis-research/  
• How to write a great hypothesis. Verywellmind website. Updated March 12, 2023. Accessed April 13, 2024. https://www.verywellmind.com/what-is-a-hypothesis-2795239  
• 15 Hypothesis examples. Helpfulprofessor.com Published September 8, 2023. Accessed March 14, 2024. https://helpfulprofessor.com/hypothesis-examples/ 
• Editage insights. What is the interconnectivity between research objectives and hypothesis? Published February 24, 2021. Accessed April 13, 2024. https://www.editage.com/insights/what-is-the-interconnectivity-between-research-objectives-and-hypothesis  
• Understanding null hypothesis testing. BCCampus open publishing. Accessed April 16, 2024. https://opentextbc.ca/researchmethods/chapter/understanding-null-hypothesis-testing/#:~:text=In%20null%20hypothesis%20testing%2C%20this,said%20to%20be%20statistically%20significant  

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