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Recent Advances in Applied Microbiology: Editorial

Letícia m. estevinho.

1 Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, 5300-252 Bragança, Portugal; tp.bpi@aluapassenav

Patrícia Combarros-Fuertes

2 Department of Food Hygiene and Technology, Faculty of Veterinary Science, University of León, Campus de Vegazana, 24071 León, Spain; se.noelinu@fmocp

Vanessa Branco Paula

The importance of microbiology has grown exponentially since the development of genomics, transcriptomics, and proteomics, making it possible to clarify microbial biogeochemical processes and their interactions with macroorganisms in both health and disease. Particular attention is being payed to applied microbiology, a discipline that deals with the application of microorganisms to specific endeavors, whose economic value is expected to exceed USD 675.2 billion by 2024. In the Special Issue “Recent Advances in Applied Microbiology”, twenty-four papers were published (four reviews and twenty original research papers), covering a wide range of subjects within applied microbiology, including: microbial pathogenesis, the health-promoting properties of microorganisms and their by-products, food conservation, the production of alcoholic beverages, bioremediation and the application of microbiology to several industrial processes.

Microorganisms, their activity and metabolites have remarkable effects on the functioning of human beings and the entire biological world, being the life-support system of the whole biosphere [ 1 ]. Microbiology, the science exploring the interactions between micro- and macro-organisms, both in health and disease, is a branch of life sciences that has grown exponentially since the establishment of genomics, transcriptomics, and proteomics. Applied microbiology is the discipline that deals with the application of microorganisms to specific endeavours, including: the optimization of animal and plant crops, the production of foods and supplements, the production of chemicals and biomaterials trough fermentation, the recovery of natural resources and energy production, the treatment of waste and the bioremediation of polluted sites [ 2 ], the production of drugs, vaccines, diagnostic tools and biosensor systems, the development of microbial therapies for dysbiosis-related diseases, and the microbial-induced modulation of biotechnological relevant cells and organisms [ 3 ].

The Special Issue entitled “Recent Advances in Applied Microbiology” aimed to document and increase the visibility of the current discoveries on this vast field. Twenty-four papers were published (four reviews and twenty original research papers) from teams situated in Asia, Europe, North America and South America, demonstrating the global impact of this thematic.

The reviews covered very pertinent topics. Malavia et al. [ 4 ] focused on human fungal pathogenesis by exploring the advantages and limitations of different molecular tools (interference ribonucleic acid, CRISPR technologies and transposon targeted mutagenesis) and in vivo models (zebrafish, the silkworm and murine), opening doors for improvements in the experimental designs of future studies. Wang et al. [ 5 ] developed an overview of the advances in the production of fungal laccase (a copper-containing oxidase enzyme) using lignocellulosic agricultural wastes. Among other aspects, this study emphasized the selection of subtracts and fungal strains and compared the traditional submerged and the solid-state fermentations. The review by Kobayashi et al. [ 6 ] described the d-amino acids produced by lactic acid bacteria. Besides being important for the flavour characteristics of lactic-acid-fermented foods, authors reported that those amino-acids have functional roles (e.g., in increasing collagen and preventing cells’ oxidation). Another team provided an update on the research on diatoms, a fundamental source of carbon, using a very interesting methodology—latent dirichlet allocation (LDA), a generative model that identifies the investigation trends in a given topic [ 7 ].

The original research articles published also covered a wide range of sectors. Across the field of medical microbiology, an innovative study explored the immunomodulatory properties of a mixture of lactic acid bacteria with probiotic features in an in vitro model of macrophage-like cells and a chemically induced colorectal cancer model [ 8 ]. Another interesting study reported the hepatoprotective properties of dietary Clostridium butyricum ; such effects were mediated by the modulation of genes and pathways associated with immunoregulation and lipid metabolism [ 9 ]. Combarros-Fuertes et al. [ 10 ] analysed the mechanisms underlying the antibacterial activity of manuka honey; it induced metabolic disruption in Staphylococcus aureus and was able to block efflux pump activity in a dose-dependent fashion in Escherichia coli. Also within this scope, Rocha et al. [ 11 ] analysed the diversity of the endophytic fungi produced by the medicinal plant Schinus terebinthifolius , the chemical compounds produced by the endophytes, and their antibacterial and antioxidant activities.

Concerning the topic of food microbiology, Cao et al. [ 12 ] described, for the first time, the promising characteristics of encapsulated Bdellovibrio powder as a bio-disinfectant against whiteleg shrimp-pathogenic vibrios. Regarding the production of alcoholic beverages, Pereira et al. [ 13 ] studied the volatile composition and sensory properties of mead produced using free and immobilized yeasts. Another Portuguese study by Esteves et al. [ 14 ] provided evidence that non-conventional yeast Saccharomycodes ludwigii has potential for wine production, either in monoculture fermentation or as a co-starter culture with Saccharomyces cerevisiae.

Within the scope of agricultural microbiology, Xu et al. [ 15 ] evaluated the effects of eucalyptus age and species on the soil microbial biomass and enzyme activities. In addition, two studies, performed by the same group, intended to explore the diversity and characteristics of rumen bacterial community [ 16 ], the digestive ability and physiological characteristics of finishing cattle after dietary changes [ 17 ].

Three studies provided advances in the field of bioremediation. Fang et al. [ 18 ] studied three denitrifying bacteria and two functional genes as potential biomarkers for total nitrogen removal. Cai et al. [ 19 ] used metagenomics analysis to explore the biodegradation related metabolism in an abnormally low dissolved inorganic carbon petroleum-contaminated aquifer. Algal biomass degradation by marine fungi isolated from the brown seaweed Fucus sp. was also studied [ 20 ]. Another team focused the production of biodiesel using E. coli with an engineered overexpressed fatty acid operon [ 21 ].

Within the framework of industrial bioprocesses, Gomes et al. [ 22 ] assessed the production of hyaluronic acid using Kluyveromyces lactis following the addition of the genes hasA and hasB (from Pasteurella multocida and Xenopus leavis , respectively). The experiments carried out by Lee et al. [ 23 ], using Deinococcus geothermalis, uncovered the pathway towards a better understanding of the physiological pros and cons of transposition phenomena. A German team investigated glucose-6-phosphate dehydrogenase genes expressed heterologously in a zwf1 deletion yeast. Considering the major role of those genes in human diseases and in plant growth, such systems are expected to possess a broad range of applications [ 24 ]. In another publication, Feng et al. [ 25 ], using a multilayer dielectric model of filamentous fugal cells, provided theoretical support for applying high-voltage pulsed electric fields to kill fungi, which may be useful for several practical applications. Aspergillus oryzae is an important industrial microorganism for the production of traditional fermented products and enzymes; the report of Shao et al. [ 26 ] provides innovative information regarding the responses of this filamentous fungi against various oxidative stresses. Last but not the least, a Korean group used Pseudomonas fluorescens to produce bacteriotoxic phospholipase A1, which is useful to efficiently degum oil [ 27 ].

Acknowledgments

We would like to thank to all the reviewers for their excellent performance and to the authors for their contributions to this Special Issue. Last but not the least, I am grateful to the Microorganisms Editorial Office for their continuous support and professional assistance.

Author Contributions

Conceptualization, L.M.E.; writing—original draft preparation, P.C.-F. and V.B.P.; writing—review and editing, L.M.E.; supervision, L.M.E. All authors have read and agreed to the published version of the manuscript.

This research received no external funding.

Conflicts of Interest

The authors declare no conflict of interest.

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ORIGINAL RESEARCH article

[frontiers in microbiology] the high-throughput solid-phase extraction of cis-cyclo(l-leu-l-pro) and cis-cyclo(l-phe-l-pro) from lactobacillus plantarum demonstrates efficacy against multidrug-resistant bacteria and influenza a (h3n2) virus provisionally accepted.

• 1 Department of Food and Nutrition, Institute of Food and Nutrition Science, College of Bio-Convergence, Eulji University, Seongnam 13135, Republic of Korea, Republic of Korea

The final, formatted version of the article will be published soon.

2,5-diketopiperazines are the simplest forms of cyclic dipeptides (CDPs) and have diverse frameworks with chiral side chains that are useful for drug development. Previous research has investigated the antimicrobial properties of proline-linked CDPs and their combinations in the culture filtrate (CF) of Lactobacillus plantarum LBP-K10 using anion exchange chromatography (AEC). However, the quantity of CDPs showcasing notable anti-influenza virus activity derived from AECs was generally lower than those originating from Lactobacillus CF. To address this issue, the study aims to propose a more efficient method for isolating CDPs and to introduce the antiviral combinations of CDPs obtained using a new method. The study employed a novel technique entailing high-throughput C18-based solidphase extraction with a methanol gradient (MeSPE). The MeSPE method involved increasing the methanol concentration from 5% to 50% in 5% increments. The methanol SPE fractions (MeSPEfs) eluted with methanol concentrations between 35% and 45% evinced substantial efficacy in inhibiting the influenza A/H3N2 virus via plaque-forming assay. MeSPEf-45, the 45% MeSPEf, exhibited exceptional efficacy in preventing viral infections in Madin-Darby kidney cells, surpassing both individual CDPs and the entire set of MeSPEfs. To identify the specific antiviral components of MeSPEf-45, all MeSPEfs were further fractionated through preparative high-performance liquid chromatography (prep-HPLC). MeSPEf-45 fractions S8 and S11 presented the highest activity against multidrug-resistant bacteria and influenza A/H3N2 virus among all MeSPEfs, with 11 common fractions. Antiviral fractions S8 and S11 were identified as proline-based CDPs, specifically cis-cyclo(L-Leu-L-Pro) and cis-cyclo(L-Phe-L-Pro), using gas chromatography-mass spectrometry. The combination of MeSPEf-45 fractions S8 and S11 displayed superior antibacterial and anti-influenza virus effects compared to the individual fractions S8 and S11. High-throughput MeSPE-derived MeSPEfs and subsequent HPLC-fractionated fractions presents an innovative approach to selectively purify large amounts of potent antimicrobial CDPs from bacterial CF. The findings also show the effectiveness of physiologically bioactive combinations that utilize fractions not containing CDP. This study provides the initial evidence demonstrating the antimicrobial properties of CDPs acquired through high-throughput SPE techniques.

Keywords: Cyclic dipeptides, cis-cyclo(L-Leu-L-Pro), cis-cyclo(L-Phe-L-Pro), solid-phase extraction, Influenza A virus, Lactobacillus plantarum LBP-K10 AEC, anion exchange chromatography, cis-cyclo(L-Leu-L-Pro), cis-cyclo(Lleucine-L-proline), cis-cyclo(L-Phe-L-Pro), cis-cyclo(L-phenylalanine-L-proline)

Received: 02 Dec 2023; Accepted: 19 Apr 2024.

Copyright: © 2024 Son, Hong, Oh and Kwak. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Min-Kyu Kwak, Department of Food and Nutrition, Institute of Food and Nutrition Science, College of Bio-Convergence, Eulji University, Seongnam 13135, Republic of Korea, Seongnam, Gyeonggi, Republic of Korea

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Brazilian indigenous nonstarter lactic acid bacteria enhance the diversification of volatile compounds in short-aged cheese

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Cristian Mauricio Barreto Pinilla, Wellington da Silva Oliveira, Aline de Oliveira Garcia, Leila Maria Spadoti, Begoña Redruello, Beatriz del Rio, Miguel Angel Alvarez, Adriana Torres Silva e Alves, Brazilian indigenous nonstarter lactic acid bacteria enhance the diversification of volatile compounds in short-aged cheese, Letters in Applied Microbiology , Volume 77, Issue 4, April 2024, ovae036, https://doi.org/10.1093/lambio/ovae036

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There is growing interest in using autochthonous lactic acid bacteria (LAB) that provide unique sensory characteristics to dairy products without affecting their safety and quality. This work studied the capacity of three Brazilian indigenous nonstarter LABs (NSLAB) to produce biogenic amines (BAs) and evaluated their effect on the volatile organic compounds (VOCs), microbial LAB communities, and physicochemical profile of short-aged cheese. Initially, the strain's potential for biosynthesis of BAs was assessed by PCR and in vitro assays. Then, a pilot-scale cheese was produced, including the NSLAB, and the microbial and VOC profiles were analyzed after 25 and 45 days of ripening. As a results, the strains did not present genes related to relevant BAs and did not produce them in vitro . During cheese ripening, the Lactococci counts were reduced, probably in the production of alcohols and acid compounds by the NSLAB. Each strain produces a unique VOC profile that changes over the ripening time without the main VOCs related to rancid or old cheese. Particularly, the use of the strain Lacticaseibacillus. paracasei ItalPN16 resulted in production of ester compounds with fruity notes. Thus, indigenous NSLAB could be a valuable tool for the enhancement and diversification of flavor in short-aged cheese.

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