process of hypothesis testing in research methodology

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Hypothesis Testing | A Step-by-Step Guide with Easy Examples

Published on November 8, 2019 by Rebecca Bevans . Revised on June 22, 2023.

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics . It is most often used by scientists to test specific predictions, called hypotheses, that arise from theories.

There are 5 main steps in hypothesis testing:

• State your research hypothesis as a null hypothesis and alternate hypothesis (H o ) and (H a  or H 1 ).
• Collect data in a way designed to test the hypothesis.
• Perform an appropriate statistical test .
• Decide whether to reject or fail to reject your null hypothesis.
• Present the findings in your results and discussion section.

Though the specific details might vary, the procedure you will use when testing a hypothesis will always follow some version of these steps.

Table of contents

Step 1: state your null and alternate hypothesis, step 2: collect data, step 3: perform a statistical test, step 4: decide whether to reject or fail to reject your null hypothesis, step 5: present your findings, other interesting articles, frequently asked questions about hypothesis testing.

After developing your initial research hypothesis (the prediction that you want to investigate), it is important to restate it as a null (H o ) and alternate (H a ) hypothesis so that you can test it mathematically.

The alternate hypothesis is usually your initial hypothesis that predicts a relationship between variables. The null hypothesis is a prediction of no relationship between the variables you are interested in.

• H 0 : Men are, on average, not taller than women. H a : Men are, on average, taller than women.

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For a statistical test to be valid , it is important to perform sampling and collect data in a way that is designed to test your hypothesis. If your data are not representative, then you cannot make statistical inferences about the population you are interested in.

There are a variety of statistical tests available, but they are all based on the comparison of within-group variance (how spread out the data is within a category) versus between-group variance (how different the categories are from one another).

If the between-group variance is large enough that there is little or no overlap between groups, then your statistical test will reflect that by showing a low p -value . This means it is unlikely that the differences between these groups came about by chance.

Alternatively, if there is high within-group variance and low between-group variance, then your statistical test will reflect that with a high p -value. This means it is likely that any difference you measure between groups is due to chance.

Your choice of statistical test will be based on the type of variables and the level of measurement of your collected data .

• an estimate of the difference in average height between the two groups.
• a p -value showing how likely you are to see this difference if the null hypothesis of no difference is true.

Based on the outcome of your statistical test, you will have to decide whether to reject or fail to reject your null hypothesis.

In most cases you will use the p -value generated by your statistical test to guide your decision. And in most cases, your predetermined level of significance for rejecting the null hypothesis will be 0.05 – that is, when there is a less than 5% chance that you would see these results if the null hypothesis were true.

In some cases, researchers choose a more conservative level of significance, such as 0.01 (1%). This minimizes the risk of incorrectly rejecting the null hypothesis ( Type I error ).

The results of hypothesis testing will be presented in the results and discussion sections of your research paper , dissertation or thesis .

In the results section you should give a brief summary of the data and a summary of the results of your statistical test (for example, the estimated difference between group means and associated p -value). In the discussion , you can discuss whether your initial hypothesis was supported by your results or not.

In the formal language of hypothesis testing, we talk about rejecting or failing to reject the null hypothesis. You will probably be asked to do this in your statistics assignments.

However, when presenting research results in academic papers we rarely talk this way. Instead, we go back to our alternate hypothesis (in this case, the hypothesis that men are on average taller than women) and state whether the result of our test did or did not support the alternate hypothesis.

If your null hypothesis was rejected, this result is interpreted as “supported the alternate hypothesis.”

These are superficial differences; you can see that they mean the same thing.

You might notice that we don’t say that we reject or fail to reject the alternate hypothesis . This is because hypothesis testing is not designed to prove or disprove anything. It is only designed to test whether a pattern we measure could have arisen spuriously, or by chance.

If we reject the null hypothesis based on our research (i.e., we find that it is unlikely that the pattern arose by chance), then we can say our test lends support to our hypothesis . But if the pattern does not pass our decision rule, meaning that it could have arisen by chance, then we say the test is inconsistent with our hypothesis .

If you want to know more about statistics , methodology , or research bias , make sure to check out some of our other articles with explanations and examples.

• Normal distribution
• Descriptive statistics
• Measures of central tendency
• Correlation coefficient

Methodology

• Cluster sampling
• Stratified sampling
• Types of interviews
• Cohort study
• Thematic analysis

Research bias

• Implicit bias
• Cognitive bias
• Survivorship bias
• Availability heuristic
• Nonresponse bias
• Regression to the mean

Hypothesis testing is a formal procedure for investigating our ideas about the world using statistics. It is used by scientists to test specific predictions, called hypotheses , by calculating how likely it is that a pattern or relationship between variables could have arisen by chance.

A hypothesis states your predictions about what your research will find. It is a tentative answer to your research question that has not yet been tested. For some research projects, you might have to write several hypotheses that address different aspects of your research question.

A hypothesis is not just a guess — it should be based on existing theories and knowledge. It also has to be testable, which means you can support or refute it through scientific research methods (such as experiments, observations and statistical analysis of data).

Null and alternative hypotheses are used in statistical hypothesis testing . The null hypothesis of a test always predicts no effect or no relationship between variables, while the alternative hypothesis states your research prediction of an effect or relationship.

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• > Statistics

What is Hypothesis Testing? Types and Methods

• Soumyaa Rawat
• Jul 23, 2021

Hypothesis Testing  

Hypothesis testing is the act of testing a hypothesis or a supposition in relation to a statistical parameter. Analysts implement hypothesis testing in order to test if a hypothesis is plausible or not. 

In data science and statistics , hypothesis testing is an important step as it involves the verification of an assumption that could help develop a statistical parameter. For instance, a researcher establishes a hypothesis assuming that the average of all odd numbers is an even number. 

In order to find the plausibility of this hypothesis, the researcher will have to test the hypothesis using hypothesis testing methods. Unlike a hypothesis that is ‘supposed’ to stand true on the basis of little or no evidence, hypothesis testing is required to have plausible evidence in order to establish that a statistical hypothesis is true. 

Perhaps this is where statistics play an important role. A number of components are involved in this process. But before understanding the process involved in hypothesis testing in research methodology, we shall first understand the types of hypotheses that are involved in the process. Let us get started! 

Types of Hypotheses

In data sampling, different types of hypothesis are involved in finding whether the tested samples test positive for a hypothesis or not. In this segment, we shall discover the different types of hypotheses and understand the role they play in hypothesis testing.

Alternative Hypothesis

Alternative Hypothesis (H1) or the research hypothesis states that there is a relationship between two variables (where one variable affects the other). The alternative hypothesis is the main driving force for hypothesis testing. 

It implies that the two variables are related to each other and the relationship that exists between them is not due to chance or coincidence. 

When the process of hypothesis testing is carried out, the alternative hypothesis is the main subject of the testing process. The analyst intends to test the alternative hypothesis and verifies its plausibility.

Null Hypothesis

The Null Hypothesis (H0) aims to nullify the alternative hypothesis by implying that there exists no relation between two variables in statistics. It states that the effect of one variable on the other is solely due to chance and no empirical cause lies behind it. 

The null hypothesis is established alongside the alternative hypothesis and is recognized as important as the latter. In hypothesis testing, the null hypothesis has a major role to play as it influences the testing against the alternative hypothesis. 

(Must read: What is ANOVA test? )

Non-Directional Hypothesis

The Non-directional hypothesis states that the relation between two variables has no direction. 

Simply put, it asserts that there exists a relation between two variables, but does not recognize the direction of effect, whether variable A affects variable B or vice versa. 

Directional Hypothesis

The Directional hypothesis, on the other hand, asserts the direction of effect of the relationship that exists between two variables. 

Herein, the hypothesis clearly states that variable A affects variable B, or vice versa. 

Statistical Hypothesis

A statistical hypothesis is a hypothesis that can be verified to be plausible on the basis of statistics. 

By using data sampling and statistical knowledge, one can determine the plausibility of a statistical hypothesis and find out if it stands true or not. 

(Related blog: z-test vs t-test )

Performing Hypothesis Testing  

Now that we have understood the types of hypotheses and the role they play in hypothesis testing, let us now move on to understand the process in a better manner. 

In hypothesis testing, a researcher is first required to establish two hypotheses - alternative hypothesis and null hypothesis in order to begin with the procedure. 

To establish these two hypotheses, one is required to study data samples, find a plausible pattern among the samples, and pen down a statistical hypothesis that they wish to test. 

A random population of samples can be drawn, to begin with hypothesis testing. Among the two hypotheses, alternative and null, only one can be verified to be true. Perhaps the presence of both hypotheses is required to make the process successful. 

At the end of the hypothesis testing procedure, either of the hypotheses will be rejected and the other one will be supported. Even though one of the two hypotheses turns out to be true, no hypothesis can ever be verified 100%. 

(Read also: Types of data sampling techniques )

Therefore, a hypothesis can only be supported based on the statistical samples and verified data. Here is a step-by-step guide for hypothesis testing.

Establish the hypotheses

First things first, one is required to establish two hypotheses - alternative and null, that will set the foundation for hypothesis testing. 

These hypotheses initiate the testing process that involves the researcher working on data samples in order to either support the alternative hypothesis or the null hypothesis. 

Generate a testing plan

Once the hypotheses have been formulated, it is now time to generate a testing plan. A testing plan or an analysis plan involves the accumulation of data samples, determining which statistic is to be considered and laying out the sample size. 

All these factors are very important while one is working on hypothesis testing.

Analyze data samples

As soon as a testing plan is ready, it is time to move on to the analysis part. Analysis of data samples involves configuring statistical values of samples, drawing them together, and deriving a pattern out of these samples. 

While analyzing the data samples, a researcher needs to determine a set of things -

Significance Level - The level of significance in hypothesis testing indicates if a statistical result could have significance if the null hypothesis stands to be true.

Testing Method - The testing method involves a type of sampling-distribution and a test statistic that leads to hypothesis testing. There are a number of testing methods that can assist in the analysis of data samples. 

Test statistic - Test statistic is a numerical summary of a data set that can be used to perform hypothesis testing.

P-value - The P-value interpretation is the probability of finding a sample statistic to be as extreme as the test statistic, indicating the plausibility of the null hypothesis. 

Infer the results

The analysis of data samples leads to the inference of results that establishes whether the alternative hypothesis stands true or not. When the P-value is less than the significance level, the null hypothesis is rejected and the alternative hypothesis turns out to be plausible. 

Methods of Hypothesis Testing

As we have already looked into different aspects of hypothesis testing, we shall now look into the different methods of hypothesis testing. All in all, there are 2 most common types of hypothesis testing methods. They are as follows -

Frequentist Hypothesis Testing

The frequentist hypothesis or the traditional approach to hypothesis testing is a hypothesis testing method that aims on making assumptions by considering current data. 

The supposed truths and assumptions are based on the current data and a set of 2 hypotheses are formulated. A very popular subtype of the frequentist approach is the Null Hypothesis Significance Testing (NHST). 

The NHST approach (involving the null and alternative hypothesis) has been one of the most sought-after methods of hypothesis testing in the field of statistics ever since its inception in the mid-1950s. 

Bayesian Hypothesis Testing

A much unconventional and modern method of hypothesis testing, the Bayesian Hypothesis Testing claims to test a particular hypothesis in accordance with the past data samples, known as prior probability, and current data that lead to the plausibility of a hypothesis. 

The result obtained indicates the posterior probability of the hypothesis. In this method, the researcher relies on ‘prior probability and posterior probability’ to conduct hypothesis testing on hand. 

On the basis of this prior probability, the Bayesian approach tests a hypothesis to be true or false. The Bayes factor, a major component of this method, indicates the likelihood ratio among the null hypothesis and the alternative hypothesis. 

The Bayes factor is the indicator of the plausibility of either of the two hypotheses that are established for hypothesis testing.  

(Also read - Introduction to Bayesian Statistics ) 

To conclude, hypothesis testing, a way to verify the plausibility of a supposed assumption can be done through different methods - the Bayesian approach or the Frequentist approach. 

Although the Bayesian approach relies on the prior probability of data samples, the frequentist approach assumes without a probability. A number of elements involved in hypothesis testing are - significance level, p-level, test statistic, and method of hypothesis testing. 

(Also read: Introduction to probability distributions )

A significant way to determine whether a hypothesis stands true or not is to verify the data samples and identify the plausible hypothesis among the null hypothesis and alternative hypothesis. 

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• Hypothesis Testing: Definition, Uses, Limitations + Examples

Hypothesis testing is as old as the scientific method and is at the heart of the research process. 

Research exists to validate or disprove assumptions about various phenomena. The process of validation involves testing and it is in this context that we will explore hypothesis testing. 

What is a Hypothesis? 

A hypothesis is a calculated prediction or assumption about a population parameter based on limited evidence. The whole idea behind hypothesis formulation is testing—this means the researcher subjects his or her calculated assumption to a series of evaluations to know whether they are true or false. 

Typically, every research starts with a hypothesis—the investigator makes a claim and experiments to prove that this claim is true or false . For instance, if you predict that students who drink milk before class perform better than those who don’t, then this becomes a hypothesis that can be confirmed or refuted using an experiment.  

Read: What is Empirical Research Study? [Examples & Method]

What are the Types of Hypotheses? 

1. simple hypothesis.

Also known as a basic hypothesis, a simple hypothesis suggests that an independent variable is responsible for a corresponding dependent variable. In other words, an occurrence of the independent variable inevitably leads to an occurrence of the dependent variable. 

Typically, simple hypotheses are considered as generally true, and they establish a causal relationship between two variables. 

Examples of Simple Hypothesis  

• Drinking soda and other sugary drinks can cause obesity. 
• Smoking cigarettes daily leads to lung cancer.

2. Complex Hypothesis

A complex hypothesis is also known as a modal. It accounts for the causal relationship between two independent variables and the resulting dependent variables. This means that the combination of the independent variables leads to the occurrence of the dependent variables . 

Examples of Complex Hypotheses  

• Adults who do not smoke and drink are less likely to develop liver-related conditions.
• Global warming causes icebergs to melt which in turn causes major changes in weather patterns.

3. Null Hypothesis

As the name suggests, a null hypothesis is formed when a researcher suspects that there’s no relationship between the variables in an observation. In this case, the purpose of the research is to approve or disapprove this assumption. 

Examples of Null Hypothesis

• This is no significant change in a student’s performance if they drink coffee or tea before classes. 
• There’s no significant change in the growth of a plant if one uses distilled water only or vitamin-rich water. 

Read: Research Report: Definition, Types + [Writing Guide]

4. Alternative Hypothesis 

To disapprove a null hypothesis, the researcher has to come up with an opposite assumption—this assumption is known as the alternative hypothesis. This means if the null hypothesis says that A is false, the alternative hypothesis assumes that A is true. 

An alternative hypothesis can be directional or non-directional depending on the direction of the difference. A directional alternative hypothesis specifies the direction of the tested relationship, stating that one variable is predicted to be larger or smaller than the null value while a non-directional hypothesis only validates the existence of a difference without stating its direction. 

Examples of Alternative Hypotheses  

• Starting your day with a cup of tea instead of a cup of coffee can make you more alert in the morning. 
• The growth of a plant improves significantly when it receives distilled water instead of vitamin-rich water. 

5. Logical Hypothesis

Logical hypotheses are some of the most common types of calculated assumptions in systematic investigations. It is an attempt to use your reasoning to connect different pieces in research and build a theory using little evidence. In this case, the researcher uses any data available to him, to form a plausible assumption that can be tested. 

Examples of Logical Hypothesis

• Waking up early helps you to have a more productive day. 
• Beings from Mars would not be able to breathe the air in the atmosphere of the Earth. 

6. Empirical Hypothesis  

After forming a logical hypothesis, the next step is to create an empirical or working hypothesis. At this stage, your logical hypothesis undergoes systematic testing to prove or disprove the assumption. An empirical hypothesis is subject to several variables that can trigger changes and lead to specific outcomes. 

Examples of Empirical Testing 

• People who eat more fish run faster than people who eat meat.
• Women taking vitamin E grow hair faster than those taking vitamin K.

7. Statistical Hypothesis

When forming a statistical hypothesis, the researcher examines the portion of a population of interest and makes a calculated assumption based on the data from this sample. A statistical hypothesis is most common with systematic investigations involving a large target audience. Here, it’s impossible to collect responses from every member of the population so you have to depend on data from your sample and extrapolate the results to the wider population. 

Examples of Statistical Hypothesis  

• 45% of students in Louisiana have middle-income parents. 
• 80% of the UK’s population gets a divorce because of irreconcilable differences.

What is Hypothesis Testing? 

Hypothesis testing is an assessment method that allows researchers to determine the plausibility of a hypothesis. It involves testing an assumption about a specific population parameter to know whether it’s true or false. These population parameters include variance, standard deviation, and median. 

Typically, hypothesis testing starts with developing a null hypothesis and then performing several tests that support or reject the null hypothesis. The researcher uses test statistics to compare the association or relationship between two or more variables. 

Explore: Research Bias: Definition, Types + Examples

Researchers also use hypothesis testing to calculate the coefficient of variation and determine if the regression relationship and the correlation coefficient are statistically significant.

How Hypothesis Testing Works

The basis of hypothesis testing is to examine and analyze the null hypothesis and alternative hypothesis to know which one is the most plausible assumption. Since both assumptions are mutually exclusive, only one can be true. In other words, the occurrence of a null hypothesis destroys the chances of the alternative coming to life, and vice-versa. 

Interesting: 21 Chrome Extensions for Academic Researchers in 2021

What Are The Stages of Hypothesis Testing?  

To successfully confirm or refute an assumption, the researcher goes through five (5) stages of hypothesis testing; 

• Determine the null hypothesis
• Specify the alternative hypothesis
• Set the significance level
• Calculate the test statistics and corresponding P-value
• Draw your conclusion
• Determine the Null Hypothesis

Like we mentioned earlier, hypothesis testing starts with creating a null hypothesis which stands as an assumption that a certain statement is false or implausible. For example, the null hypothesis (H0) could suggest that different subgroups in the research population react to a variable in the same way. 

• Specify the Alternative Hypothesis

Once you know the variables for the null hypothesis, the next step is to determine the alternative hypothesis. The alternative hypothesis counters the null assumption by suggesting the statement or assertion is true. Depending on the purpose of your research, the alternative hypothesis can be one-sided or two-sided. 

Using the example we established earlier, the alternative hypothesis may argue that the different sub-groups react differently to the same variable based on several internal and external factors. 

• Set the Significance Level

Many researchers create a 5% allowance for accepting the value of an alternative hypothesis, even if the value is untrue. This means that there is a 0.05 chance that one would go with the value of the alternative hypothesis, despite the truth of the null hypothesis. 

Something to note here is that the smaller the significance level, the greater the burden of proof needed to reject the null hypothesis and support the alternative hypothesis.

Explore: What is Data Interpretation? + [Types, Method & Tools]

• Calculate the Test Statistics and Corresponding P-Value 

Test statistics in hypothesis testing allow you to compare different groups between variables while the p-value accounts for the probability of obtaining sample statistics if your null hypothesis is true. In this case, your test statistics can be the mean, median and similar parameters. 

If your p-value is 0.65, for example, then it means that the variable in your hypothesis will happen 65 in100 times by pure chance. Use this formula to determine the p-value for your data: 

• Draw Your Conclusions

After conducting a series of tests, you should be able to agree or refute the hypothesis based on feedback and insights from your sample data.  

Applications of Hypothesis Testing in Research

Hypothesis testing isn’t only confined to numbers and calculations; it also has several real-life applications in business, manufacturing, advertising, and medicine. 

In a factory or other manufacturing plants, hypothesis testing is an important part of quality and production control before the final products are approved and sent out to the consumer. 

During ideation and strategy development, C-level executives use hypothesis testing to evaluate their theories and assumptions before any form of implementation. For example, they could leverage hypothesis testing to determine whether or not some new advertising campaign, marketing technique, etc. causes increased sales. 

In addition, hypothesis testing is used during clinical trials to prove the efficacy of a drug or new medical method before its approval for widespread human usage. 

What is an Example of Hypothesis Testing?

An employer claims that her workers are of above-average intelligence. She takes a random sample of 20 of them and gets the following results: 

Mean IQ Scores: 110

Standard Deviation: 15 

Mean Population IQ: 100

Step 1: Using the value of the mean population IQ, we establish the null hypothesis as 100.

Step 2: State that the alternative hypothesis is greater than 100.

Step 3: State the alpha level as 0.05 or 5% 

Step 4: Find the rejection region area (given by your alpha level above) from the z-table. An area of .05 is equal to a z-score of 1.645.

Step 5: Calculate the test statistics using this formula

Z = (110–100) ÷ (15÷√20) 

10 ÷ 3.35 = 2.99 

If the value of the test statistics is higher than the value of the rejection region, then you should reject the null hypothesis. If it is less, then you cannot reject the null. 

In this case, 2.99 > 1.645 so we reject the null. 

Importance/Benefits of Hypothesis Testing 

The most significant benefit of hypothesis testing is it allows you to evaluate the strength of your claim or assumption before implementing it in your data set. Also, hypothesis testing is the only valid method to prove that something “is or is not”. Other benefits include: 

• Hypothesis testing provides a reliable framework for making any data decisions for your population of interest. 
• It helps the researcher to successfully extrapolate data from the sample to the larger population. 
• Hypothesis testing allows the researcher to determine whether the data from the sample is statistically significant. 
• Hypothesis testing is one of the most important processes for measuring the validity and reliability of outcomes in any systematic investigation. 
• It helps to provide links to the underlying theory and specific research questions.

Criticism and Limitations of Hypothesis Testing

Several limitations of hypothesis testing can affect the quality of data you get from this process. Some of these limitations include: 

• The interpretation of a p-value for observation depends on the stopping rule and definition of multiple comparisons. This makes it difficult to calculate since the stopping rule is subject to numerous interpretations, plus “multiple comparisons” are unavoidably ambiguous. 
• Conceptual issues often arise in hypothesis testing, especially if the researcher merges Fisher and Neyman-Pearson’s methods which are conceptually distinct. 
• In an attempt to focus on the statistical significance of the data, the researcher might ignore the estimation and confirmation by repeated experiments.
• Hypothesis testing can trigger publication bias, especially when it requires statistical significance as a criterion for publication.
• When used to detect whether a difference exists between groups, hypothesis testing can trigger absurd assumptions that affect the reliability of your observation.

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• How it works

Hypothesis Testing – A Complete Guide with Examples

Published by Alvin Nicolas at August 14th, 2021 , Revised On October 26, 2023

In statistics, hypothesis testing is a critical tool. It allows us to make informed decisions about populations based on sample data. Whether you are a researcher trying to prove a scientific point, a marketer analysing A/B test results, or a manufacturer ensuring quality control, hypothesis testing plays a pivotal role. This guide aims to introduce you to the concept and walk you through real-world examples.

What is a Hypothesis and a Hypothesis Testing?

A hypothesis is considered a belief or assumption that has to be accepted, rejected, proved or disproved. In contrast, a research hypothesis is a research question for a researcher that has to be proven correct or incorrect through investigation.

What is Hypothesis Testing?

Hypothesis testing  is a scientific method used for making a decision and drawing conclusions by using a statistical approach. It is used to suggest new ideas by testing theories to know whether or not the sample data supports research. A research hypothesis is a predictive statement that has to be tested using scientific methods that join an independent variable to a dependent variable.  

Example: The academic performance of student A is better than student B

Characteristics of the Hypothesis to be Tested

A hypothesis should be:

• Clear and precise
• Capable of being tested
• Able to relate to a variable
• Stated in simple terms
• Consistent with known facts
• Limited in scope and specific
• Tested in a limited timeframe
• Explain the facts in detail

What is a Null Hypothesis and Alternative Hypothesis?

A  null hypothesis  is a hypothesis when there is no significant relationship between the dependent and the participants’ independent  variables . 

In simple words, it’s a hypothesis that has been put forth but hasn’t been proved as yet. A researcher aims to disprove the theory. The abbreviation “Ho” is used to denote a null hypothesis.

If you want to compare two methods and assume that both methods are equally good, this assumption is considered the null hypothesis.

Example: In an automobile trial, you feel that the new vehicle’s mileage is similar to the previous model of the car, on average. You can write it as: Ho: there is no difference between the mileage of both vehicles. If your findings don’t support your hypothesis and you get opposite results, this outcome will be considered an alternative hypothesis.

If you assume that one method is better than another method, then it’s considered an alternative hypothesis. The alternative hypothesis is the theory that a researcher seeks to prove and is typically denoted by H1 or HA.

If you support a null hypothesis, it means you’re not supporting the alternative hypothesis. Similarly, if you reject a null hypothesis, it means you are recommending the alternative hypothesis.

Example: In an automobile trial, you feel that the new vehicle’s mileage is better than the previous model of the vehicle. You can write it as; Ha: the two vehicles have different mileage. On average/ the fuel consumption of the new vehicle model is better than the previous model.

If a null hypothesis is rejected during the hypothesis test, even if it’s true, then it is considered as a type-I error. On the other hand, if you don’t dismiss a hypothesis, even if it’s false because you could not identify its falseness, it’s considered a type-II error.

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How to Conduct Hypothesis Testing?

Here is a step-by-step guide on how to conduct hypothesis testing.

Step 1: State the Null and Alternative Hypothesis

Once you develop a research hypothesis, it’s important to state it is as a Null hypothesis (Ho) and an Alternative hypothesis (Ha) to test it statistically.

A null hypothesis is a preferred choice as it provides the opportunity to test the theory. In contrast, you can accept the alternative hypothesis when the null hypothesis has been rejected.

Example: You want to identify a relationship between obesity of men and women and the modern living style. You develop a hypothesis that women, on average, gain weight quickly compared to men. Then you write it as: Ho: Women, on average, don’t gain weight quickly compared to men. Ha: Women, on average, gain weight quickly compared to men.

Step 2: Data Collection

Hypothesis testing follows the statistical method, and statistics are all about data. It’s challenging to gather complete information about a specific population you want to study. You need to  gather the data  obtained through a large number of samples from a specific population. 

Example: Suppose you want to test the difference in the rate of obesity between men and women. You should include an equal number of men and women in your sample. Then investigate various aspects such as their lifestyle, eating patterns and profession, and any other variables that may influence average weight. You should also determine your study’s scope, whether it applies to a specific group of population or worldwide population. You can use available information from various places, countries, and regions.

Step 3: Select Appropriate Statistical Test

There are many  types of statistical tests , but we discuss the most two common types below, such as One-sided and two-sided tests.

Note: Your choice of the type of test depends on the purpose of your study 

One-sided Test

In the one-sided test, the values of rejecting a null hypothesis are located in one tail of the probability distribution. The set of values is less or higher than the critical value of the test. It is also called a one-tailed test of significance.

Example: If you want to test that all mangoes in a basket are ripe. You can write it as: Ho: All mangoes in the basket, on average, are ripe. If you find all ripe mangoes in the basket, the null hypothesis you developed will be true.

Two-sided Test

In the two-sided test, the values of rejecting a null hypothesis are located on both tails of the probability distribution. The set of values is less or higher than the first critical value of the test and higher than the second critical value test. It is also called a two-tailed test of significance. 

Example: Nothing can be explicitly said whether all mangoes are ripe in the basket. If you reject the null hypothesis (Ho: All mangoes in the basket, on average, are ripe), then it means all mangoes in the basket are not likely to be ripe. A few mangoes could be raw as well.

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Step 4: Select the Level of Significance

When you reject a null hypothesis, even if it’s true during a statistical hypothesis, it is considered the  significance level . It is the probability of a type one error. The significance should be as minimum as possible to avoid the type-I error, which is considered severe and should be avoided. 

If the significance level is minimum, then it prevents the researchers from false claims. 

The significance level is denoted by  P,  and it has given the value of 0.05 (P=0.05)

If the P-Value is less than 0.05, then the difference will be significant. If the P-value is higher than 0.05, then the difference is non-significant.

Example: Suppose you apply a one-sided test to test whether women gain weight quickly compared to men. You get to know about the average weight between men and women and the factors promoting weight gain.

Step 5: Find out Whether the Null Hypothesis is Rejected or Supported

After conducting a statistical test, you should identify whether your null hypothesis is rejected or accepted based on the test results. It would help if you observed the P-value for this.

Example: If you find the P-value of your test is less than 0.5/5%, then you need to reject your null hypothesis (Ho: Women, on average, don’t gain weight quickly compared to men). On the other hand, if a null hypothesis is rejected, then it means the alternative hypothesis might be true (Ha: Women, on average, gain weight quickly compared to men. If you find your test’s P-value is above 0.5/5%, then it means your null hypothesis is true.

Step 6: Present the Outcomes of your Study

The final step is to present the  outcomes of your study . You need to ensure whether you have met the objectives of your research or not. 

In the discussion section and  conclusion , you can present your findings by using supporting evidence and conclude whether your null hypothesis was rejected or supported.

In the result section, you can summarise your study’s outcomes, including the average difference and P-value of the two groups.

If we talk about the findings, our study your results will be as follows:

Example: In the study of identifying whether women gain weight quickly compared to men, we found the P-value is less than 0.5. Hence, we can reject the null hypothesis (Ho: Women, on average, don’t gain weight quickly than men) and conclude that women may likely gain weight quickly than men.

Did you know in your academic paper you should not mention whether you have accepted or rejected the null hypothesis? 

Always remember that you either conclude to reject Ho in favor of Haor   do not reject Ho . It would help if you never rejected  Ha  or even  accept Ha .

Suppose your null hypothesis is rejected in the hypothesis testing. If you conclude  reject Ho in favor of Haor   do not reject Ho,  then it doesn’t mean that the null hypothesis is true. It only means that there is a lack of evidence against Ho in favour of Ha. If your null hypothesis is not true, then the alternative hypothesis is likely to be true.

Example: We found that the P-value is less than 0.5. Hence, we can conclude reject Ho in favour of Ha (Ho: Women, on average, don’t gain weight quickly than men) reject Ho in favour of Ha. However, rejected in favour of Ha means (Ha: women may likely to gain weight quickly than men)

Frequently Asked Questions

What are the 3 types of hypothesis test.

The 3 types of hypothesis tests are:

• One-Sample Test : Compare sample data to a known population value.
• Two-Sample Test : Compare means between two sample groups.
• ANOVA : Analyze variance among multiple groups to determine significant differences.

What is a hypothesis?

A hypothesis is a proposed explanation or prediction about a phenomenon, often based on observations. It serves as a starting point for research or experimentation, providing a testable statement that can either be supported or refuted through data and analysis. In essence, it’s an educated guess that drives scientific inquiry.

What are null hypothesis?

A null hypothesis (often denoted as H0) suggests that there is no effect or difference in a study or experiment. It represents a default position or status quo. Statistical tests evaluate data to determine if there’s enough evidence to reject this null hypothesis.

What is the probability value?

The probability value, or p-value, is a measure used in statistics to determine the significance of an observed effect. It indicates the probability of obtaining the observed results, or more extreme, if the null hypothesis were true. A small p-value (typically <0.05) suggests evidence against the null hypothesis, warranting its rejection.

What is p value?

The p-value is a fundamental concept in statistical hypothesis testing. It represents the probability of observing a test statistic as extreme, or more so, than the one calculated from sample data, assuming the null hypothesis is true. A low p-value suggests evidence against the null, possibly justifying its rejection.

What is a t test?

A t-test is a statistical test used to compare the means of two groups. It determines if observed differences between the groups are statistically significant or if they likely occurred by chance. Commonly applied in research, there are different t-tests, including independent, paired, and one-sample, tailored to various data scenarios.

When to reject null hypothesis?

Reject the null hypothesis when the test statistic falls into a predefined rejection region or when the p-value is less than the chosen significance level (commonly 0.05). This suggests that the observed data is unlikely under the null hypothesis, indicating evidence for the alternative hypothesis. Always consider the study’s context.

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Hypothesis Testing

When you conduct a piece of quantitative research, you are inevitably attempting to answer a research question or hypothesis that you have set. One method of evaluating this research question is via a process called hypothesis testing , which is sometimes also referred to as significance testing . Since there are many facets to hypothesis testing, we start with the example we refer to throughout this guide.

An example of a lecturer's dilemma

Two statistics lecturers, Sarah and Mike, think that they use the best method to teach their students. Each lecturer has 50 statistics students who are studying a graduate degree in management. In Sarah's class, students have to attend one lecture and one seminar class every week, whilst in Mike's class students only have to attend one lecture. Sarah thinks that seminars, in addition to lectures, are an important teaching method in statistics, whilst Mike believes that lectures are sufficient by themselves and thinks that students are better off solving problems by themselves in their own time. This is the first year that Sarah has given seminars, but since they take up a lot of her time, she wants to make sure that she is not wasting her time and that seminars improve her students' performance.

The research hypothesis

The first step in hypothesis testing is to set a research hypothesis. In Sarah and Mike's study, the aim is to examine the effect that two different teaching methods – providing both lectures and seminar classes (Sarah), and providing lectures by themselves (Mike) – had on the performance of Sarah's 50 students and Mike's 50 students. More specifically, they want to determine whether performance is different between the two different teaching methods. Whilst Mike is skeptical about the effectiveness of seminars, Sarah clearly believes that giving seminars in addition to lectures helps her students do better than those in Mike's class. This leads to the following research hypothesis:

Before moving onto the second step of the hypothesis testing process, we need to take you on a brief detour to explain why you need to run hypothesis testing at all. This is explained next.

Sample to population

If you have measured individuals (or any other type of "object") in a study and want to understand differences (or any other type of effect), you can simply summarize the data you have collected. For example, if Sarah and Mike wanted to know which teaching method was the best, they could simply compare the performance achieved by the two groups of students – the group of students that took lectures and seminar classes, and the group of students that took lectures by themselves – and conclude that the best method was the teaching method which resulted in the highest performance. However, this is generally of only limited appeal because the conclusions could only apply to students in this study. However, if those students were representative of all statistics students on a graduate management degree, the study would have wider appeal.

In statistics terminology, the students in the study are the sample and the larger group they represent (i.e., all statistics students on a graduate management degree) is called the population . Given that the sample of statistics students in the study are representative of a larger population of statistics students, you can use hypothesis testing to understand whether any differences or effects discovered in the study exist in the population. In layman's terms, hypothesis testing is used to establish whether a research hypothesis extends beyond those individuals examined in a single study.

Another example could be taking a sample of 200 breast cancer sufferers in order to test a new drug that is designed to eradicate this type of cancer. As much as you are interested in helping these specific 200 cancer sufferers, your real goal is to establish that the drug works in the population (i.e., all breast cancer sufferers).

As such, by taking a hypothesis testing approach, Sarah and Mike want to generalize their results to a population rather than just the students in their sample. However, in order to use hypothesis testing, you need to re-state your research hypothesis as a null and alternative hypothesis. Before you can do this, it is best to consider the process/structure involved in hypothesis testing and what you are measuring. This structure is presented on the next page .

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A Beginner’s Guide to Hypothesis Testing in Business

• 30 Mar 2021

Becoming a more data-driven decision-maker can bring several benefits to your organization, enabling you to identify new opportunities to pursue and threats to abate. Rather than allowing subjective thinking to guide your business strategy, backing your decisions with data can empower your company to become more innovative and, ultimately, profitable.

If you’re new to data-driven decision-making, you might be wondering how data translates into business strategy. The answer lies in generating a hypothesis and verifying or rejecting it based on what various forms of data tell you.

Below is a look at hypothesis testing and the role it plays in helping businesses become more data-driven.

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What Is Hypothesis Testing?

To understand what hypothesis testing is, it’s important first to understand what a hypothesis is.

A hypothesis or hypothesis statement seeks to explain why something has happened, or what might happen, under certain conditions. It can also be used to understand how different variables relate to each other. Hypotheses are often written as if-then statements; for example, “If this happens, then this will happen.”

Hypothesis testing , then, is a statistical means of testing an assumption stated in a hypothesis. While the specific methodology leveraged depends on the nature of the hypothesis and data available, hypothesis testing typically uses sample data to extrapolate insights about a larger population.

Hypothesis Testing in Business

When it comes to data-driven decision-making, there’s a certain amount of risk that can mislead a professional. This could be due to flawed thinking or observations, incomplete or inaccurate data , or the presence of unknown variables. The danger in this is that, if major strategic decisions are made based on flawed insights, it can lead to wasted resources, missed opportunities, and catastrophic outcomes.

The real value of hypothesis testing in business is that it allows professionals to test their theories and assumptions before putting them into action. This essentially allows an organization to verify its analysis is correct before committing resources to implement a broader strategy.

As one example, consider a company that wishes to launch a new marketing campaign to revitalize sales during a slow period. Doing so could be an incredibly expensive endeavor, depending on the campaign’s size and complexity. The company, therefore, may wish to test the campaign on a smaller scale to understand how it will perform.

In this example, the hypothesis that’s being tested would fall along the lines of: “If the company launches a new marketing campaign, then it will translate into an increase in sales.” It may even be possible to quantify how much of a lift in sales the company expects to see from the effort. Pending the results of the pilot campaign, the business would then know whether it makes sense to roll it out more broadly.

Related: 9 Fundamental Data Science Skills for Business Professionals

Key Considerations for Hypothesis Testing

1. alternative hypothesis and null hypothesis.

In hypothesis testing, the hypothesis that’s being tested is known as the alternative hypothesis . Often, it’s expressed as a correlation or statistical relationship between variables. The null hypothesis , on the other hand, is a statement that’s meant to show there’s no statistical relationship between the variables being tested. It’s typically the exact opposite of whatever is stated in the alternative hypothesis.

For example, consider a company’s leadership team that historically and reliably sees $12 million in monthly revenue. They want to understand if reducing the price of their services will attract more customers and, in turn, increase revenue.

In this case, the alternative hypothesis may take the form of a statement such as: “If we reduce the price of our flagship service by five percent, then we’ll see an increase in sales and realize revenues greater than $12 million in the next month.”

The null hypothesis, on the other hand, would indicate that revenues wouldn’t increase from the base of $12 million, or might even decrease.

Check out the video below about the difference between an alternative and a null hypothesis, and subscribe to our YouTube channel for more explainer content.

2. Significance Level and P-Value

Statistically speaking, if you were to run the same scenario 100 times, you’d likely receive somewhat different results each time. If you were to plot these results in a distribution plot, you’d see the most likely outcome is at the tallest point in the graph, with less likely outcomes falling to the right and left of that point.

With this in mind, imagine you’ve completed your hypothesis test and have your results, which indicate there may be a correlation between the variables you were testing. To understand your results' significance, you’ll need to identify a p-value for the test, which helps note how confident you are in the test results.

In statistics, the p-value depicts the probability that, assuming the null hypothesis is correct, you might still observe results that are at least as extreme as the results of your hypothesis test. The smaller the p-value, the more likely the alternative hypothesis is correct, and the greater the significance of your results.

3. One-Sided vs. Two-Sided Testing

When it’s time to test your hypothesis, it’s important to leverage the correct testing method. The two most common hypothesis testing methods are one-sided and two-sided tests , or one-tailed and two-tailed tests, respectively.

Typically, you’d leverage a one-sided test when you have a strong conviction about the direction of change you expect to see due to your hypothesis test. You’d leverage a two-sided test when you’re less confident in the direction of change.

4. Sampling

To perform hypothesis testing in the first place, you need to collect a sample of data to be analyzed. Depending on the question you’re seeking to answer or investigate, you might collect samples through surveys, observational studies, or experiments.

A survey involves asking a series of questions to a random population sample and recording self-reported responses.

Observational studies involve a researcher observing a sample population and collecting data as it occurs naturally, without intervention.

Finally, an experiment involves dividing a sample into multiple groups, one of which acts as the control group. For each non-control group, the variable being studied is manipulated to determine how the data collected differs from that of the control group.

Learn How to Perform Hypothesis Testing

Hypothesis testing is a complex process involving different moving pieces that can allow an organization to effectively leverage its data and inform strategic decisions.

If you’re interested in better understanding hypothesis testing and the role it can play within your organization, one option is to complete a course that focuses on the process. Doing so can lay the statistical and analytical foundation you need to succeed.

Do you want to learn more about hypothesis testing? Explore Business Analytics —one of our online business essentials courses —and download our Beginner’s Guide to Data & Analytics .

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1.2: The 7-Step Process of Statistical Hypothesis Testing

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• Penn State's Department of Statistics
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We will cover the seven steps one by one.

Step 1: State the Null Hypothesis

The null hypothesis can be thought of as the opposite of the "guess" the researchers made: in this example, the biologist thinks the plant height will be different for the fertilizers. So the null would be that there will be no difference among the groups of plants. Specifically, in more statistical language the null for an ANOVA is that the means are the same. We state the null hypothesis as: \[H_{0}: \ \mu_{1} = \mu_{2} = \ldots = \mu_{T}\] for \(T\) levels of an experimental treatment.

Why do we do this? Why not simply test the working hypothesis directly? The answer lies in the Popperian Principle of Falsification. Karl Popper (a philosopher) discovered that we can't conclusively confirm a hypothesis, but we can conclusively negate one. So we set up a null hypothesis which is effectively the opposite of the working hypothesis. The hope is that based on the strength of the data, we will be able to negate or reject the null hypothesis and accept an alternative hypothesis. In other words, we usually see the working hypothesis in \(H_{A}\).

Step 2: State the Alternative Hypothesis

\[H_{A}: \ \text{treatment level means not all equal}\]

The reason we state the alternative hypothesis this way is that if the null is rejected, there are many possibilities.

For example, \(\mu_{1} \neq \mu_{2} = \ldots = \mu_{T}\) is one possibility, as is \(\mu_{1} = \mu_{2} \neq \mu_{3} = \ldots = \mu_{T}\). Many people make the mistake of stating the alternative hypothesis as \(mu_{1} \neq mu_{2} \neq \ldots \neq \mu_{T}\), which says that every mean differs from every other mean. This is a possibility, but only one of many possibilities. To cover all alternative outcomes, we resort to a verbal statement of "not all equal" and then follow up with mean comparisons to find out where differences among means exist. In our example, this means that fertilizer 1 may result in plants that are really tall, but fertilizers 2, 3, and the plants with no fertilizers don't differ from one another. A simpler way of thinking about this is that at least one mean is different from all others.

Step 3: Set \(\alpha\)

If we look at what can happen in a hypothesis test, we can construct the following contingency table:

You should be familiar with type I and type II errors from your introductory course. It is important to note that we want to set \(\alpha\) before the experiment ( a priori ) because the Type I error is the more grievous error to make. The typical value of \(\alpha\) is 0.05, establishing a 95% confidence level. For this course, we will assume \(\alpha\) =0.05, unless stated otherwise.

Step 4: Collect Data

Remember the importance of recognizing whether data is collected through an experimental design or observational study.

Step 5: Calculate a test statistic

For categorical treatment level means, we use an \(F\) statistic, named after R.A. Fisher. We will explore the mechanics of computing the \(F\) statistic beginning in Chapter 2. The \(F\) value we get from the data is labeled \(F_{\text{calculated}}\).

Step 6: Construct Acceptance / Rejection regions

As with all other test statistics, a threshold (critical) value of \(F\) is established. This \(F\) value can be obtained from statistical tables or software and is referred to as \(F_{\text{critical}}\) or \(F_{\alpha}\). As a reminder, this critical value is the minimum value for the test statistic (in this case the F test) for us to be able to reject the null.

The \(F\) distribution, \(F_{\alpha}\), and the location of acceptance and rejection regions are shown in the graph below:

Step 7: Based on steps 5 and 6, draw a conclusion about H0

If the \(F_{\text{\calculated}}\) from the data is larger than the \(F_{\alpha}\), then you are in the rejection region and you can reject the null hypothesis with \((1 - \alpha)\) level of confidence.

Note that modern statistical software condenses steps 6 and 7 by providing a \(p\)-value. The \(p\)-value here is the probability of getting an \(F_{\text{calculated}}\) even greater than what you observe assuming the null hypothesis is true. If by chance, the \(F_{\text{calculated}} = F_{\alpha}\), then the \(p\)-value would exactly equal \(\alpha\). With larger \(F_{\text{calculated}}\) values, we move further into the rejection region and the \(p\) - value becomes less than \(\alpha\). So the decision rule is as follows:

If the \(p\) - value obtained from the ANOVA is less than \(\alpha\), then reject \(H_{0}\) and accept \(H_{A}\).

If you are not familiar with this material, we suggest that you review course materials from your basic statistics course.

Hypothesis Testing for Research – Complete Guide With Example

Published 16 October, 2023

Hypothesis testing is a statistical technique used for deciding whether to reject the null hypothesis. Hypothesis testing helps researchers know whether or not they are on the correct path for their research project and can save them from time wasted pursuing wrong leads. Before starting any type of hypothesis test, it is vital that you have an idea of what your variable will be and how to measure it accurately. This post will cover some of the most common types of hypothesis tests and provide examples, as well as discuss what they are designed to test.

What is a hypothesis in research?

Hypothesis in research is basically a statement that helps you to define the relationship between different types of variables for your study. You can consider the hypothesis as the expectation about the things that can happen during research. The main objective of including hypotheses in research is to get the answer to research questions .

For example, while watering a plant you expect that if you will give more amount of water and sunlight to the plant, it would grow big soon.

What are the different types of hypothesis?

There are different types of hypothesis but here, we will discuss mainly two types of hypothesis, these are :

Null hypothesis: It is a hypothesis, where you can not expect variations. The null hypothesis states that there is no relationship between the dependent and independent variables. For instance, there is no significant relationship between compensation policy and employee satisfaction. In research null hypothesis is denoted as H0. Alternative hypothesis: In the Alternative hypothesis, variations are expected. This is something that a researcher aims to indirectly verify by stating their assumption, which states that there exists a significant linkage between population parameters. For instance, there is a significant relationship between companies’ compensation policy and employee satisfaction. It denoted as H a  or H 1.

What is hypothesis testing?

Hypothesis testing is basically a statistical procedure that is researcher performs with the purpose of determining whether there are chances of a specific hypothesis to be true. In simple words, by using the statistics you can tests whether your prediction about the population parameters is correct or not.

Or we can say, Hypothesis testing is a research method that uses statistical tools to prove or disprove a theory. The hypothesis is an idea about what might be true, and the goal of hypothesis testing is to provide evidence for or against that idea.

What are the uses of hypothesis testing?

• You can use hypothesis testing for making assumptions about the outcome of the hypothesis on sample information that you have gathered from a large population.
• Students can utilize it for analyzing the strong proof which you have collected from the sample.
• Hypothesis testing provides the structure for making various assumptions about the population.

How to conduct hypothesis testing?

The test procedure or the rule is based upon a test statistic and a rejection region. The process of testing the hypothesis consists of the following steps:

Step 1 – Specification of hypothesis

It is the first step in hypothesis testing where you need to clearly define the null and alternative hypotheses . While stating the hypothesis you need to make sure that it is mutually exclusive which means that if one statement is true then the other should be false. While defining the variables you need to confirm that the statement representing the relationship between two or more variables.

Step 2 – Selection of significance level

This is a stage where you need to set the significance level. The significance level (denoted by the Greek letter alpha— a) is generally set at 0.05. This means that there is a 5% chance that you will accept your alternative hypothesis when your null hypothesis is actually true. The smaller the significance level, the greater the burden of proof needed to reject the null hypothesis, or in other words, to support the alternative hypothesis.

Step 3 – Collection of information

It is a stage where you will require accumulating all the facts about the research topic. The process of making statistical inferences should be done in a way that is designed to test your hypothesis. If you do not design the sampling methods and data collection appropriately, then it will make no sense for you to try drawing conclusions about any population at all!

Step 4 – Determination of critical values

This is a phase where you need to determine P-value. It is basically a value that the researcher utilizes for determining statistical importance in hypothesis tests. Determination of P-value is important as it will help you in evaluating the extent up to which the hypothesis statement given by you is true. It will also help you in analyzing the extent up to which the facts which you have gathered are compatible with the null hypothesis.

There are basically two types of P-value these are :

• High: High P-value indicates that the facts which you have collected are highly compatible with the null hypothesis.
• Low: It is the P-value that represents that the information which the researcher has to accumulate is not at all compatible with the null hypothesis .

Comparison of critical value and making a judgment: Here, you need to make a comparison between P values, and on the basis of the same you need to make a decision.

Step 5 – Drawing a conclusion

With the conclusion stage, we either accept or reject the null hypothesis. The decision is based on computed values of the test statistics and whether it lies in the acceptance region or rejection region respectively. If the computed value of the test statistic falls in the acceptance region (it means the computed value is less than the critical value), the null hypothesis is accepted. On the contrary, if the computed value of the test statistic is greater than the critical value, the computed value of the statistic falls in the rejection region, and the null hypothesis is rejected.

Hypothesis Testing Example

A manager in pipe manufacturing needs to ensure that the diameters of pipes manufactured by the machine are 5 cm. Then you as a manager is needed to undergo the following phases of the hypothesis test these are:

• Establishment for criteria: At this stage, you as a manager will need to design the hypothesis. The null hypothesis here could be every pipe has a diameter of 5 cm. AS manager needs to confirm that every pipe should be diameter 5 cm then he can make a selection from an alternative hypothesis which could be The population mean is fewer than the target, the mean of the population which manager has select is greater in comparison to the target. A third alternative hypothesis could be men population is completely different from the target.  In such a case, the manager can select two side alternative hypotheses. An alternative hypothesis which manager could select is the mean population of all pipes is not 5 cm.
• Selection of significance level: At this stage, you can select the most basically used significance level which is 0.05.
• Collection of facts: It is a stage where you will require gathering information about pipes and their diameters.
• Comparison between P values: After completion of the hypothesis test you will obtain P-value. Suppose here the P-value which results in 0.04 which is less than the significance level that is 0.05. on the basis of comparison between P values, you need to make a decision whether to reject the null hypothesis or not.

Read Also: Thematic Analysis for Research

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Hypothesis to Be Tested: Definition and 4 Steps for Testing with Example

What Is Hypothesis Testing?

Hypothesis testing, sometimes called significance testing, is an act in statistics whereby an analyst tests an assumption regarding a population parameter. The methodology employed by the analyst depends on the nature of the data used and the reason for the analysis.

Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data. Such data may come from a larger population, or from a data-generating process. The word "population" will be used for both of these cases in the following descriptions.

Key Takeaways

• Hypothesis testing is used to assess the plausibility of a hypothesis by using sample data.
• The test provides evidence concerning the plausibility of the hypothesis, given the data.
• Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed.
• The four steps of hypothesis testing include stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result.

How Hypothesis Testing Works

In hypothesis testing, an  analyst  tests a statistical sample, with the goal of providing evidence on the plausibility of the null hypothesis.

Statistical analysts test a hypothesis by measuring and examining a random sample of the population being analyzed. All analysts use a random population sample to test two different hypotheses: the null hypothesis and the alternative hypothesis.

The null hypothesis is usually a hypothesis of equality between population parameters; e.g., a null hypothesis may state that the population mean return is equal to zero. The alternative hypothesis is effectively the opposite of a null hypothesis (e.g., the population mean return is not equal to zero). Thus, they are mutually exclusive , and only one can be true. However, one of the two hypotheses will always be true.

The null hypothesis is a statement about a population parameter, such as the population mean, that is assumed to be true.

4 Steps of Hypothesis Testing

All hypotheses are tested using a four-step process:

• The first step is for the analyst to state the hypotheses.
• The second step is to formulate an analysis plan, which outlines how the data will be evaluated.
• The third step is to carry out the plan and analyze the sample data.
• The final step is to analyze the results and either reject the null hypothesis, or state that the null hypothesis is plausible, given the data.

Real-World Example of Hypothesis Testing

If, for example, a person wants to test that a penny has exactly a 50% chance of landing on heads, the null hypothesis would be that 50% is correct, and the alternative hypothesis would be that 50% is not correct.

Mathematically, the null hypothesis would be represented as Ho: P = 0.5. The alternative hypothesis would be denoted as "Ha" and be identical to the null hypothesis, except with the equal sign struck-through, meaning that it does not equal 50%.

A random sample of 100 coin flips is taken, and the null hypothesis is then tested. If it is found that the 100 coin flips were distributed as 40 heads and 60 tails, the analyst would assume that a penny does not have a 50% chance of landing on heads and would reject the null hypothesis and accept the alternative hypothesis.

If, on the other hand, there were 48 heads and 52 tails, then it is plausible that the coin could be fair and still produce such a result. In cases such as this where the null hypothesis is "accepted," the analyst states that the difference between the expected results (50 heads and 50 tails) and the observed results (48 heads and 52 tails) is "explainable by chance alone."

Some staticians attribute the first hypothesis tests to satirical writer John Arbuthnot in 1710, who studied male and female births in England after observing that in nearly every year, male births exceeded female births by a slight proportion. Arbuthnot calculated that the probability of this happening by chance was small, and therefore it was due to “divine providence.”

What is Hypothesis Testing?

Hypothesis testing refers to a process used by analysts to assess the plausibility of a hypothesis by using sample data. In hypothesis testing, statisticians formulate two hypotheses: the null hypothesis and the alternative hypothesis. A null hypothesis determines there is no difference between two groups or conditions, while the alternative hypothesis determines that there is a difference. Researchers evaluate the statistical significance of the test based on the probability that the null hypothesis is true.

What are the Four Key Steps Involved in Hypothesis Testing?

Hypothesis testing begins with an analyst stating two hypotheses, with only one that can be right. The analyst then formulates an analysis plan, which outlines how the data will be evaluated. Next, they move to the testing phase and analyze the sample data. Finally, the analyst analyzes the results and either rejects the null hypothesis or states that the null hypothesis is plausible, given the data.

What are the Benefits of Hypothesis Testing?

Hypothesis testing helps assess the accuracy of new ideas or theories by testing them against data. This allows researchers to determine whether the evidence supports their hypothesis, helping to avoid false claims and conclusions. Hypothesis testing also provides a framework for decision-making based on data rather than personal opinions or biases. By relying on statistical analysis, hypothesis testing helps to reduce the effects of chance and confounding variables, providing a robust framework for making informed conclusions.

What are the Limitations of Hypothesis Testing?

Hypothesis testing relies exclusively on data and doesn’t provide a comprehensive understanding of the subject being studied. Additionally, the accuracy of the results depends on the quality of the available data and the statistical methods used. Inaccurate data or inappropriate hypothesis formulation may lead to incorrect conclusions or failed tests. Hypothesis testing can also lead to errors, such as analysts either accepting or rejecting a null hypothesis when they shouldn’t have. These errors may result in false conclusions or missed opportunities to identify significant patterns or relationships in the data.

The Bottom Line

Hypothesis testing refers to a statistical process that helps researchers and/or analysts determine the reliability of a study. By using a well-formulated hypothesis and set of statistical tests, individuals or businesses can make inferences about the population that they are studying and draw conclusions based on the data presented. There are different types of hypothesis testing, each with their own set of rules and procedures. However, all hypothesis testing methods have the same four step process, which includes stating the hypotheses, formulating an analysis plan, analyzing the sample data, and analyzing the result. Hypothesis testing plays a vital part of the scientific process, helping to test assumptions and make better data-based decisions.

Sage. " Introduction to Hypothesis Testing. " Page 4.

Elder Research. " Who Invented the Null Hypothesis? "

Formplus. " Hypothesis Testing: Definition, Uses, Limitations and Examples. "

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The Craft of Writing a Strong Hypothesis

Table of Contents

Writing a hypothesis is one of the essential elements of a scientific research paper. It needs to be to the point, clearly communicating what your research is trying to accomplish. A blurry, drawn-out, or complexly-structured hypothesis can confuse your readers. Or worse, the editor and peer reviewers.

A captivating hypothesis is not too intricate. This blog will take you through the process so that, by the end of it, you have a better idea of how to convey your research paper's intent in just one sentence.

What is a Hypothesis?

The first step in your scientific endeavor, a hypothesis, is a strong, concise statement that forms the basis of your research. It is not the same as a thesis statement , which is a brief summary of your research paper .

The sole purpose of a hypothesis is to predict your paper's findings, data, and conclusion. It comes from a place of curiosity and intuition . When you write a hypothesis, you're essentially making an educated guess based on scientific prejudices and evidence, which is further proven or disproven through the scientific method.

The reason for undertaking research is to observe a specific phenomenon. A hypothesis, therefore, lays out what the said phenomenon is. And it does so through two variables, an independent and dependent variable.

The independent variable is the cause behind the observation, while the dependent variable is the effect of the cause. A good example of this is “mixing red and blue forms purple.” In this hypothesis, mixing red and blue is the independent variable as you're combining the two colors at your own will. The formation of purple is the dependent variable as, in this case, it is conditional to the independent variable.

Different Types of Hypotheses‌

Types of hypotheses

Some would stand by the notion that there are only two types of hypotheses: a Null hypothesis and an Alternative hypothesis. While that may have some truth to it, it would be better to fully distinguish the most common forms as these terms come up so often, which might leave you out of context.

Apart from Null and Alternative, there are Complex, Simple, Directional, Non-Directional, Statistical, and Associative and casual hypotheses. They don't necessarily have to be exclusive, as one hypothesis can tick many boxes, but knowing the distinctions between them will make it easier for you to construct your own.

1. Null hypothesis

A null hypothesis proposes no relationship between two variables. Denoted by H 0 , it is a negative statement like “Attending physiotherapy sessions does not affect athletes' on-field performance.” Here, the author claims physiotherapy sessions have no effect on on-field performances. Even if there is, it's only a coincidence.

2. Alternative hypothesis

Considered to be the opposite of a null hypothesis, an alternative hypothesis is donated as H1 or Ha. It explicitly states that the dependent variable affects the independent variable. A good  alternative hypothesis example is “Attending physiotherapy sessions improves athletes' on-field performance.” or “Water evaporates at 100 °C. ” The alternative hypothesis further branches into directional and non-directional.

• Directional hypothesis: A hypothesis that states the result would be either positive or negative is called directional hypothesis. It accompanies H1 with either the ‘<' or ‘>' sign.
• Non-directional hypothesis: A non-directional hypothesis only claims an effect on the dependent variable. It does not clarify whether the result would be positive or negative. The sign for a non-directional hypothesis is ‘≠.'

3. Simple hypothesis

A simple hypothesis is a statement made to reflect the relation between exactly two variables. One independent and one dependent. Consider the example, “Smoking is a prominent cause of lung cancer." The dependent variable, lung cancer, is dependent on the independent variable, smoking.

4. Complex hypothesis

In contrast to a simple hypothesis, a complex hypothesis implies the relationship between multiple independent and dependent variables. For instance, “Individuals who eat more fruits tend to have higher immunity, lesser cholesterol, and high metabolism.” The independent variable is eating more fruits, while the dependent variables are higher immunity, lesser cholesterol, and high metabolism.

5. Associative and casual hypothesis

Associative and casual hypotheses don't exhibit how many variables there will be. They define the relationship between the variables. In an associative hypothesis, changing any one variable, dependent or independent, affects others. In a casual hypothesis, the independent variable directly affects the dependent.

6. Empirical hypothesis

Also referred to as the working hypothesis, an empirical hypothesis claims a theory's validation via experiments and observation. This way, the statement appears justifiable and different from a wild guess.

Say, the hypothesis is “Women who take iron tablets face a lesser risk of anemia than those who take vitamin B12.” This is an example of an empirical hypothesis where the researcher  the statement after assessing a group of women who take iron tablets and charting the findings.

7. Statistical hypothesis

The point of a statistical hypothesis is to test an already existing hypothesis by studying a population sample. Hypothesis like “44% of the Indian population belong in the age group of 22-27.” leverage evidence to prove or disprove a particular statement.

Characteristics of a Good Hypothesis

Writing a hypothesis is essential as it can make or break your research for you. That includes your chances of getting published in a journal. So when you're designing one, keep an eye out for these pointers:

• A research hypothesis has to be simple yet clear to look justifiable enough.
• It has to be testable — your research would be rendered pointless if too far-fetched into reality or limited by technology.
• It has to be precise about the results —what you are trying to do and achieve through it should come out in your hypothesis.
• A research hypothesis should be self-explanatory, leaving no doubt in the reader's mind.
• If you are developing a relational hypothesis, you need to include the variables and establish an appropriate relationship among them.
• A hypothesis must keep and reflect the scope for further investigations and experiments.

Separating a Hypothesis from a Prediction

Outside of academia, hypothesis and prediction are often used interchangeably. In research writing, this is not only confusing but also incorrect. And although a hypothesis and prediction are guesses at their core, there are many differences between them.

A hypothesis is an educated guess or even a testable prediction validated through research. It aims to analyze the gathered evidence and facts to define a relationship between variables and put forth a logical explanation behind the nature of events.

Predictions are assumptions or expected outcomes made without any backing evidence. They are more fictionally inclined regardless of where they originate from.

For this reason, a hypothesis holds much more weight than a prediction. It sticks to the scientific method rather than pure guesswork. "Planets revolve around the Sun." is an example of a hypothesis as it is previous knowledge and observed trends. Additionally, we can test it through the scientific method.

Whereas "COVID-19 will be eradicated by 2030." is a prediction. Even though it results from past trends, we can't prove or disprove it. So, the only way this gets validated is to wait and watch if COVID-19 cases end by 2030.

Finally, How to Write a Hypothesis

Quick tips on writing a hypothesis

1.  Be clear about your research question

A hypothesis should instantly address the research question or the problem statement. To do so, you need to ask a question. Understand the constraints of your undertaken research topic and then formulate a simple and topic-centric problem. Only after that can you develop a hypothesis and further test for evidence.

2. Carry out a recce

Once you have your research's foundation laid out, it would be best to conduct preliminary research. Go through previous theories, academic papers, data, and experiments before you start curating your research hypothesis. It will give you an idea of your hypothesis's viability or originality.

Making use of references from relevant research papers helps draft a good research hypothesis. SciSpace Discover offers a repository of over 270 million research papers to browse through and gain a deeper understanding of related studies on a particular topic. Additionally, you can use SciSpace Copilot , your AI research assistant, for reading any lengthy research paper and getting a more summarized context of it. A hypothesis can be formed after evaluating many such summarized research papers. Copilot also offers explanations for theories and equations, explains paper in simplified version, allows you to highlight any text in the paper or clip math equations and tables and provides a deeper, clear understanding of what is being said. This can improve the hypothesis by helping you identify potential research gaps.

3. Create a 3-dimensional hypothesis

Variables are an essential part of any reasonable hypothesis. So, identify your independent and dependent variable(s) and form a correlation between them. The ideal way to do this is to write the hypothetical assumption in the ‘if-then' form. If you use this form, make sure that you state the predefined relationship between the variables.

In another way, you can choose to present your hypothesis as a comparison between two variables. Here, you must specify the difference you expect to observe in the results.

4. Write the first draft

Now that everything is in place, it's time to write your hypothesis. For starters, create the first draft. In this version, write what you expect to find from your research.

Clearly separate your independent and dependent variables and the link between them. Don't fixate on syntax at this stage. The goal is to ensure your hypothesis addresses the issue.

5. Proof your hypothesis

After preparing the first draft of your hypothesis, you need to inspect it thoroughly. It should tick all the boxes, like being concise, straightforward, relevant, and accurate. Your final hypothesis has to be well-structured as well.

Research projects are an exciting and crucial part of being a scholar. And once you have your research question, you need a great hypothesis to begin conducting research. Thus, knowing how to write a hypothesis is very important.

Now that you have a firmer grasp on what a good hypothesis constitutes, the different kinds there are, and what process to follow, you will find it much easier to write your hypothesis, which ultimately helps your research.

Now it's easier than ever to streamline your research workflow with SciSpace Discover . Its integrated, comprehensive end-to-end platform for research allows scholars to easily discover, write and publish their research and fosters collaboration.

It includes everything you need, including a repository of over 270 million research papers across disciplines, SEO-optimized summaries and public profiles to show your expertise and experience.

If you found these tips on writing a research hypothesis useful, head over to our blog on Statistical Hypothesis Testing to learn about the top researchers, papers, and institutions in this domain.

Frequently Asked Questions (FAQs)

1. what is the definition of hypothesis.

According to the Oxford dictionary, a hypothesis is defined as “An idea or explanation of something that is based on a few known facts, but that has not yet been proved to be true or correct”.

2. What is an example of hypothesis?

The hypothesis is a statement that proposes a relationship between two or more variables. An example: "If we increase the number of new users who join our platform by 25%, then we will see an increase in revenue."

3. What is an example of null hypothesis?

A null hypothesis is a statement that there is no relationship between two variables. The null hypothesis is written as H0. The null hypothesis states that there is no effect. For example, if you're studying whether or not a particular type of exercise increases strength, your null hypothesis will be "there is no difference in strength between people who exercise and people who don't."

4. What are the types of research?

• Fundamental research

• Applied research

• Qualitative research

• Quantitative research

• Mixed research

• Exploratory research

• Longitudinal research

• Cross-sectional research

• Field research

• Laboratory research

• Fixed research

• Flexible research

• Action research

• Policy research

• Classification research

• Comparative research

• Causal research

• Inductive research

• Deductive research

5. How to write a hypothesis?

• Your hypothesis should be able to predict the relationship and outcome.

• Avoid wordiness by keeping it simple and brief.

• Your hypothesis should contain observable and testable outcomes.

• Your hypothesis should be relevant to the research question.

6. What are the 2 types of hypothesis?

• Null hypotheses are used to test the claim that "there is no difference between two groups of data".

• Alternative hypotheses test the claim that "there is a difference between two data groups".

7. Difference between research question and research hypothesis?

A research question is a broad, open-ended question you will try to answer through your research. A hypothesis is a statement based on prior research or theory that you expect to be true due to your study. Example - Research question: What are the factors that influence the adoption of the new technology? Research hypothesis: There is a positive relationship between age, education and income level with the adoption of the new technology.

8. What is plural for hypothesis?

The plural of hypothesis is hypotheses. Here's an example of how it would be used in a statement, "Numerous well-considered hypotheses are presented in this part, and they are supported by tables and figures that are well-illustrated."

9. What is the red queen hypothesis?

The red queen hypothesis in evolutionary biology states that species must constantly evolve to avoid extinction because if they don't, they will be outcompeted by other species that are evolving. Leigh Van Valen first proposed it in 1973; since then, it has been tested and substantiated many times.

10. Who is known as the father of null hypothesis?

The father of the null hypothesis is Sir Ronald Fisher. He published a paper in 1925 that introduced the concept of null hypothesis testing, and he was also the first to use the term itself.

11. When to reject null hypothesis?

You need to find a significant difference between your two populations to reject the null hypothesis. You can determine that by running statistical tests such as an independent sample t-test or a dependent sample t-test. You should reject the null hypothesis if the p-value is less than 0.05.

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Principles of Research Methodology pp 1–14 Cite as

Overview of the Research Process

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Research is a rigorous problem-solving process whose ultimate goal is the discovery of new knowledge. Research may include the description of a new phenomenon, definition of a new relationship, development of a new model, or application of an existing principle or procedure to a new context. Research is systematic, logical, empirical, reductive, replicable and transmittable, and generalizable. Research can be classified according to a variety of dimensions: basic, applied, or translational; hypothesis generating or hypothesis testing; retrospective or prospective; longitudinal or cross-sectional; observational or experimental; and quantitative or qualitative. The ultimate success of a research project is heavily dependent on adequate planning.

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Formulating Hypotheses for Different Study Designs

Durga prasanna misra.

1 Department of Clinical Immunology and Rheumatology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India.

Armen Yuri Gasparyan

2 Departments of Rheumatology and Research and Development, Dudley Group NHS Foundation Trust (Teaching Trust of the University of Birmingham, UK), Russells Hall Hospital, Dudley, UK.

Olena Zimba

3 Department of Internal Medicine #2, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine.

Marlen Yessirkepov

4 Department of Biology and Biochemistry, South Kazakhstan Medical Academy, Shymkent, Kazakhstan.

Vikas Agarwal

George d. kitas.

5 Centre for Epidemiology versus Arthritis, University of Manchester, Manchester, UK.

Generating a testable working hypothesis is the first step towards conducting original research. Such research may prove or disprove the proposed hypothesis. Case reports, case series, online surveys and other observational studies, clinical trials, and narrative reviews help to generate hypotheses. Observational and interventional studies help to test hypotheses. A good hypothesis is usually based on previous evidence-based reports. Hypotheses without evidence-based justification and a priori ideas are not received favourably by the scientific community. Original research to test a hypothesis should be carefully planned to ensure appropriate methodology and adequate statistical power. While hypotheses can challenge conventional thinking and may be controversial, they should not be destructive. A hypothesis should be tested by ethically sound experiments with meaningful ethical and clinical implications. The coronavirus disease 2019 pandemic has brought into sharp focus numerous hypotheses, some of which were proven (e.g. effectiveness of corticosteroids in those with hypoxia) while others were disproven (e.g. ineffectiveness of hydroxychloroquine and ivermectin).

Graphical Abstract

DEFINING WORKING AND STANDALONE SCIENTIFIC HYPOTHESES

Science is the systematized description of natural truths and facts. Routine observations of existing life phenomena lead to the creative thinking and generation of ideas about mechanisms of such phenomena and related human interventions. Such ideas presented in a structured format can be viewed as hypotheses. After generating a hypothesis, it is necessary to test it to prove its validity. Thus, hypothesis can be defined as a proposed mechanism of a naturally occurring event or a proposed outcome of an intervention. 1 , 2

Hypothesis testing requires choosing the most appropriate methodology and adequately powering statistically the study to be able to “prove” or “disprove” it within predetermined and widely accepted levels of certainty. This entails sample size calculation that often takes into account previously published observations and pilot studies. 2 , 3 In the era of digitization, hypothesis generation and testing may benefit from the availability of numerous platforms for data dissemination, social networking, and expert validation. Related expert evaluations may reveal strengths and limitations of proposed ideas at early stages of post-publication promotion, preventing the implementation of unsupported controversial points. 4

Thus, hypothesis generation is an important initial step in the research workflow, reflecting accumulating evidence and experts' stance. In this article, we overview the genesis and importance of scientific hypotheses and their relevance in the era of the coronavirus disease 2019 (COVID-19) pandemic.

DO WE NEED HYPOTHESES FOR ALL STUDY DESIGNS?

Broadly, research can be categorized as primary or secondary. In the context of medicine, primary research may include real-life observations of disease presentations and outcomes. Single case descriptions, which often lead to new ideas and hypotheses, serve as important starting points or justifications for case series and cohort studies. The importance of case descriptions is particularly evident in the context of the COVID-19 pandemic when unique, educational case reports have heralded a new era in clinical medicine. 5

Case series serve similar purpose to single case reports, but are based on a slightly larger quantum of information. Observational studies, including online surveys, describe the existing phenomena at a larger scale, often involving various control groups. Observational studies include variable-scale epidemiological investigations at different time points. Interventional studies detail the results of therapeutic interventions.

Secondary research is based on already published literature and does not directly involve human or animal subjects. Review articles are generated by secondary research. These could be systematic reviews which follow methods akin to primary research but with the unit of study being published papers rather than humans or animals. Systematic reviews have a rigid structure with a mandatory search strategy encompassing multiple databases, systematic screening of search results against pre-defined inclusion and exclusion criteria, critical appraisal of study quality and an optional component of collating results across studies quantitatively to derive summary estimates (meta-analysis). 6 Narrative reviews, on the other hand, have a more flexible structure. Systematic literature searches to minimise bias in selection of articles are highly recommended but not mandatory. 7 Narrative reviews are influenced by the authors' viewpoint who may preferentially analyse selected sets of articles. 8

In relation to primary research, case studies and case series are generally not driven by a working hypothesis. Rather, they serve as a basis to generate a hypothesis. Observational or interventional studies should have a hypothesis for choosing research design and sample size. The results of observational and interventional studies further lead to the generation of new hypotheses, testing of which forms the basis of future studies. Review articles, on the other hand, may not be hypothesis-driven, but form fertile ground to generate future hypotheses for evaluation. Fig. 1 summarizes which type of studies are hypothesis-driven and which lead on to hypothesis generation.

STANDARDS OF WORKING AND SCIENTIFIC HYPOTHESES

A review of the published literature did not enable the identification of clearly defined standards for working and scientific hypotheses. It is essential to distinguish influential versus not influential hypotheses, evidence-based hypotheses versus a priori statements and ideas, ethical versus unethical, or potentially harmful ideas. The following points are proposed for consideration while generating working and scientific hypotheses. 1 , 2 Table 1 summarizes these points.

Evidence-based data

A scientific hypothesis should have a sound basis on previously published literature as well as the scientist's observations. Randomly generated (a priori) hypotheses are unlikely to be proven. A thorough literature search should form the basis of a hypothesis based on published evidence. 7

Unless a scientific hypothesis can be tested, it can neither be proven nor be disproven. Therefore, a scientific hypothesis should be amenable to testing with the available technologies and the present understanding of science.

Supported by pilot studies

If a hypothesis is based purely on a novel observation by the scientist in question, it should be grounded on some preliminary studies to support it. For example, if a drug that targets a specific cell population is hypothesized to be useful in a particular disease setting, then there must be some preliminary evidence that the specific cell population plays a role in driving that disease process.

Testable by ethical studies

The hypothesis should be testable by experiments that are ethically acceptable. 9 For example, a hypothesis that parachutes reduce mortality from falls from an airplane cannot be tested using a randomized controlled trial. 10 This is because it is obvious that all those jumping from a flying plane without a parachute would likely die. Similarly, the hypothesis that smoking tobacco causes lung cancer cannot be tested by a clinical trial that makes people take up smoking (since there is considerable evidence for the health hazards associated with smoking). Instead, long-term observational studies comparing outcomes in those who smoke and those who do not, as was performed in the landmark epidemiological case control study by Doll and Hill, 11 are more ethical and practical.

Balance between scientific temper and controversy

Novel findings, including novel hypotheses, particularly those that challenge established norms, are bound to face resistance for their wider acceptance. Such resistance is inevitable until the time such findings are proven with appropriate scientific rigor. However, hypotheses that generate controversy are generally unwelcome. For example, at the time the pandemic of human immunodeficiency virus (HIV) and AIDS was taking foot, there were numerous deniers that refused to believe that HIV caused AIDS. 12 , 13 Similarly, at a time when climate change is causing catastrophic changes to weather patterns worldwide, denial that climate change is occurring and consequent attempts to block climate change are certainly unwelcome. 14 The denialism and misinformation during the COVID-19 pandemic, including unfortunate examples of vaccine hesitancy, are more recent examples of controversial hypotheses not backed by science. 15 , 16 An example of a controversial hypothesis that was a revolutionary scientific breakthrough was the hypothesis put forth by Warren and Marshall that Helicobacter pylori causes peptic ulcers. Initially, the hypothesis that a microorganism could cause gastritis and gastric ulcers faced immense resistance. When the scientists that proposed the hypothesis themselves ingested H. pylori to induce gastritis in themselves, only then could they convince the wider world about their hypothesis. Such was the impact of the hypothesis was that Barry Marshall and Robin Warren were awarded the Nobel Prize in Physiology or Medicine in 2005 for this discovery. 17 , 18

DISTINGUISHING THE MOST INFLUENTIAL HYPOTHESES

Influential hypotheses are those that have stood the test of time. An archetype of an influential hypothesis is that proposed by Edward Jenner in the eighteenth century that cowpox infection protects against smallpox. While this observation had been reported for nearly a century before this time, it had not been suitably tested and publicised until Jenner conducted his experiments on a young boy by demonstrating protection against smallpox after inoculation with cowpox. 19 These experiments were the basis for widespread smallpox immunization strategies worldwide in the 20th century which resulted in the elimination of smallpox as a human disease today. 20

Other influential hypotheses are those which have been read and cited widely. An example of this is the hygiene hypothesis proposing an inverse relationship between infections in early life and allergies or autoimmunity in adulthood. An analysis reported that this hypothesis had been cited more than 3,000 times on Scopus. 1

LESSONS LEARNED FROM HYPOTHESES AMIDST THE COVID-19 PANDEMIC

The COVID-19 pandemic devastated the world like no other in recent memory. During this period, various hypotheses emerged, understandably so considering the public health emergency situation with innumerable deaths and suffering for humanity. Within weeks of the first reports of COVID-19, aberrant immune system activation was identified as a key driver of organ dysfunction and mortality in this disease. 21 Consequently, numerous drugs that suppress the immune system or abrogate the activation of the immune system were hypothesized to have a role in COVID-19. 22 One of the earliest drugs hypothesized to have a benefit was hydroxychloroquine. Hydroxychloroquine was proposed to interfere with Toll-like receptor activation and consequently ameliorate the aberrant immune system activation leading to pathology in COVID-19. 22 The drug was also hypothesized to have a prophylactic role in preventing infection or disease severity in COVID-19. It was also touted as a wonder drug for the disease by many prominent international figures. However, later studies which were well-designed randomized controlled trials failed to demonstrate any benefit of hydroxychloroquine in COVID-19. 23 , 24 , 25 , 26 Subsequently, azithromycin 27 , 28 and ivermectin 29 were hypothesized as potential therapies for COVID-19, but were not supported by evidence from randomized controlled trials. The role of vitamin D in preventing disease severity was also proposed, but has not been proven definitively until now. 30 , 31 On the other hand, randomized controlled trials identified the evidence supporting dexamethasone 32 and interleukin-6 pathway blockade with tocilizumab as effective therapies for COVID-19 in specific situations such as at the onset of hypoxia. 33 , 34 Clues towards the apparent effectiveness of various drugs against severe acute respiratory syndrome coronavirus 2 in vitro but their ineffectiveness in vivo have recently been identified. Many of these drugs are weak, lipophilic bases and some others induce phospholipidosis which results in apparent in vitro effectiveness due to non-specific off-target effects that are not replicated inside living systems. 35 , 36

Another hypothesis proposed was the association of the routine policy of vaccination with Bacillus Calmette-Guerin (BCG) with lower deaths due to COVID-19. This hypothesis emerged in the middle of 2020 when COVID-19 was still taking foot in many parts of the world. 37 , 38 Subsequently, many countries which had lower deaths at that time point went on to have higher numbers of mortality, comparable to other areas of the world. Furthermore, the hypothesis that BCG vaccination reduced COVID-19 mortality was a classic example of ecological fallacy. Associations between population level events (ecological studies; in this case, BCG vaccination and COVID-19 mortality) cannot be directly extrapolated to the individual level. Furthermore, such associations cannot per se be attributed as causal in nature, and can only serve to generate hypotheses that need to be tested at the individual level. 39

IS TRADITIONAL PEER REVIEW EFFICIENT FOR EVALUATION OF WORKING AND SCIENTIFIC HYPOTHESES?

Traditionally, publication after peer review has been considered the gold standard before any new idea finds acceptability amongst the scientific community. Getting a work (including a working or scientific hypothesis) reviewed by experts in the field before experiments are conducted to prove or disprove it helps to refine the idea further as well as improve the experiments planned to test the hypothesis. 40 A route towards this has been the emergence of journals dedicated to publishing hypotheses such as the Central Asian Journal of Medical Hypotheses and Ethics. 41 Another means of publishing hypotheses is through registered research protocols detailing the background, hypothesis, and methodology of a particular study. If such protocols are published after peer review, then the journal commits to publishing the completed study irrespective of whether the study hypothesis is proven or disproven. 42 In the post-pandemic world, online research methods such as online surveys powered via social media channels such as Twitter and Instagram might serve as critical tools to generate as well as to preliminarily test the appropriateness of hypotheses for further evaluation. 43 , 44

Some radical hypotheses might be difficult to publish after traditional peer review. These hypotheses might only be acceptable by the scientific community after they are tested in research studies. Preprints might be a way to disseminate such controversial and ground-breaking hypotheses. 45 However, scientists might prefer to keep their hypotheses confidential for the fear of plagiarism of ideas, avoiding online posting and publishing until they have tested the hypotheses.

SUGGESTIONS ON GENERATING AND PUBLISHING HYPOTHESES

Publication of hypotheses is important, however, a balance is required between scientific temper and controversy. Journal editors and reviewers might keep in mind these specific points, summarized in Table 2 and detailed hereafter, while judging the merit of hypotheses for publication. Keeping in mind the ethical principle of primum non nocere, a hypothesis should be published only if it is testable in a manner that is ethically appropriate. 46 Such hypotheses should be grounded in reality and lend themselves to further testing to either prove or disprove them. It must be considered that subsequent experiments to prove or disprove a hypothesis have an equal chance of failing or succeeding, akin to tossing a coin. A pre-conceived belief that a hypothesis is unlikely to be proven correct should not form the basis of rejection of such a hypothesis for publication. In this context, hypotheses generated after a thorough literature search to identify knowledge gaps or based on concrete clinical observations on a considerable number of patients (as opposed to random observations on a few patients) are more likely to be acceptable for publication by peer-reviewed journals. Also, hypotheses should be considered for publication or rejection based on their implications for science at large rather than whether the subsequent experiments to test them end up with results in favour of or against the original hypothesis.

Hypotheses form an important part of the scientific literature. The COVID-19 pandemic has reiterated the importance and relevance of hypotheses for dealing with public health emergencies and highlighted the need for evidence-based and ethical hypotheses. A good hypothesis is testable in a relevant study design, backed by preliminary evidence, and has positive ethical and clinical implications. General medical journals might consider publishing hypotheses as a specific article type to enable more rapid advancement of science.

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

• Data curation: Gasparyan AY, Misra DP, Zimba O, Yessirkepov M, Agarwal V, Kitas GD.

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HYPOTHESIS TESTING IN RESEARCH METHODOLOGY: A REVIEW

Hypothesis is usually considered as the principal instrument in research. It plays a major role in research. Its main function is to suggest new experiments and observations. It occupies a very small space in the thesis. A researcher cannot proceed in the research work without formulating one or more than one hypothesis. Hypothesis brings clarity, specificity and focus to a research problem. There are two types of hypothesis which are called the Null Hypothesis and the Alternative Hypothesis. There are four steps in hypothesis testing. After getting the results, the researcher tests whether the collected facts support the hypothesis formulated or not. There exist a number of statistical tools like ttest, F-test, chi-square test, D-W test, etc., to test the validity of hypothesis. Hypothesis testing shows whether the hypothesis should be accepted or rejected. It has been rightly said that “You torture the data until they confess”. This paper include the introduction, steps of hypothesis testing, function of hypothesis and testing of hypothesis.

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Home » Research Process – Steps, Examples and Tips

Research Process – Steps, Examples and Tips

Table of Contents

Research Process

Definition:

Research Process is a systematic and structured approach that involves the collection, analysis, and interpretation of data or information to answer a specific research question or solve a particular problem.

Research Process Steps

Research Process Steps are as follows:

Identify the Research Question or Problem

This is the first step in the research process. It involves identifying a problem or question that needs to be addressed. The research question should be specific, relevant, and focused on a particular area of interest.

Conduct a Literature Review

Once the research question has been identified, the next step is to conduct a literature review. This involves reviewing existing research and literature on the topic to identify any gaps in knowledge or areas where further research is needed. A literature review helps to provide a theoretical framework for the research and also ensures that the research is not duplicating previous work.

Formulate a Hypothesis or Research Objectives

Based on the research question and literature review, the researcher can formulate a hypothesis or research objectives. A hypothesis is a statement that can be tested to determine its validity, while research objectives are specific goals that the researcher aims to achieve through the research.

Design a Research Plan and Methodology

This step involves designing a research plan and methodology that will enable the researcher to collect and analyze data to test the hypothesis or achieve the research objectives. The research plan should include details on the sample size, data collection methods, and data analysis techniques that will be used.

Collect and Analyze Data

This step involves collecting and analyzing data according to the research plan and methodology. Data can be collected through various methods, including surveys, interviews, observations, or experiments. The data analysis process involves cleaning and organizing the data, applying statistical and analytical techniques to the data, and interpreting the results.

Interpret the Findings and Draw Conclusions

After analyzing the data, the researcher must interpret the findings and draw conclusions. This involves assessing the validity and reliability of the results and determining whether the hypothesis was supported or not. The researcher must also consider any limitations of the research and discuss the implications of the findings.

Communicate the Results

Finally, the researcher must communicate the results of the research through a research report, presentation, or publication. The research report should provide a detailed account of the research process, including the research question, literature review, research methodology, data analysis, findings, and conclusions. The report should also include recommendations for further research in the area.

Review and Revise

The research process is an iterative one, and it is important to review and revise the research plan and methodology as necessary. Researchers should assess the quality of their data and methods, reflect on their findings, and consider areas for improvement.

Ethical Considerations

Throughout the research process, ethical considerations must be taken into account. This includes ensuring that the research design protects the welfare of research participants, obtaining informed consent, maintaining confidentiality and privacy, and avoiding any potential harm to participants or their communities.

Dissemination and Application

The final step in the research process is to disseminate the findings and apply the research to real-world settings. Researchers can share their findings through academic publications, presentations at conferences, or media coverage. The research can be used to inform policy decisions, develop interventions, or improve practice in the relevant field.

Research Process Example

Following is a Research Process Example:

Research Question : What are the effects of a plant-based diet on athletic performance in high school athletes?

Step 1: Background Research Conduct a literature review to gain a better understanding of the existing research on the topic. Read academic articles and research studies related to plant-based diets, athletic performance, and high school athletes.

Step 2: Develop a Hypothesis Based on the literature review, develop a hypothesis that a plant-based diet positively affects athletic performance in high school athletes.

Step 3: Design the Study Design a study to test the hypothesis. Decide on the study population, sample size, and research methods. For this study, you could use a survey to collect data on dietary habits and athletic performance from a sample of high school athletes who follow a plant-based diet and a sample of high school athletes who do not follow a plant-based diet.

Step 4: Collect Data Distribute the survey to the selected sample and collect data on dietary habits and athletic performance.

Step 5: Analyze Data Use statistical analysis to compare the data from the two samples and determine if there is a significant difference in athletic performance between those who follow a plant-based diet and those who do not.

Step 6 : Interpret Results Interpret the results of the analysis in the context of the research question and hypothesis. Discuss any limitations or potential biases in the study design.

Step 7: Draw Conclusions Based on the results, draw conclusions about whether a plant-based diet has a significant effect on athletic performance in high school athletes. If the hypothesis is supported by the data, discuss potential implications and future research directions.

Step 8: Communicate Findings Communicate the findings of the study in a clear and concise manner. Use appropriate language, visuals, and formats to ensure that the findings are understood and valued.

Applications of Research Process

The research process has numerous applications across a wide range of fields and industries. Some examples of applications of the research process include:

• Scientific research: The research process is widely used in scientific research to investigate phenomena in the natural world and develop new theories or technologies. This includes fields such as biology, chemistry, physics, and environmental science.
• Social sciences : The research process is commonly used in social sciences to study human behavior, social structures, and institutions. This includes fields such as sociology, psychology, anthropology, and economics.
• Education: The research process is used in education to study learning processes, curriculum design, and teaching methodologies. This includes research on student achievement, teacher effectiveness, and educational policy.
• Healthcare: The research process is used in healthcare to investigate medical conditions, develop new treatments, and evaluate healthcare interventions. This includes fields such as medicine, nursing, and public health.
• Business and industry : The research process is used in business and industry to study consumer behavior, market trends, and develop new products or services. This includes market research, product development, and customer satisfaction research.
• Government and policy : The research process is used in government and policy to evaluate the effectiveness of policies and programs, and to inform policy decisions. This includes research on social welfare, crime prevention, and environmental policy.

Purpose of Research Process

The purpose of the research process is to systematically and scientifically investigate a problem or question in order to generate new knowledge or solve a problem. The research process enables researchers to:

• Identify gaps in existing knowledge: By conducting a thorough literature review, researchers can identify gaps in existing knowledge and develop research questions that address these gaps.
• Collect and analyze data : The research process provides a structured approach to collecting and analyzing data. Researchers can use a variety of research methods, including surveys, experiments, and interviews, to collect data that is valid and reliable.
• Test hypotheses : The research process allows researchers to test hypotheses and make evidence-based conclusions. Through the systematic analysis of data, researchers can draw conclusions about the relationships between variables and develop new theories or models.
• Solve problems: The research process can be used to solve practical problems and improve real-world outcomes. For example, researchers can develop interventions to address health or social problems, evaluate the effectiveness of policies or programs, and improve organizational processes.
• Generate new knowledge : The research process is a key way to generate new knowledge and advance understanding in a given field. By conducting rigorous and well-designed research, researchers can make significant contributions to their field and help to shape future research.

Tips for Research Process

Here are some tips for the research process:

• Start with a clear research question : A well-defined research question is the foundation of a successful research project. It should be specific, relevant, and achievable within the given time frame and resources.
• Conduct a thorough literature review: A comprehensive literature review will help you to identify gaps in existing knowledge, build on previous research, and avoid duplication. It will also provide a theoretical framework for your research.
• Choose appropriate research methods: Select research methods that are appropriate for your research question, objectives, and sample size. Ensure that your methods are valid, reliable, and ethical.
• Be organized and systematic: Keep detailed notes throughout the research process, including your research plan, methodology, data collection, and analysis. This will help you to stay organized and ensure that you don’t miss any important details.
• Analyze data rigorously: Use appropriate statistical and analytical techniques to analyze your data. Ensure that your analysis is valid, reliable, and transparent.
• I nterpret results carefully : Interpret your results in the context of your research question and objectives. Consider any limitations or potential biases in your research design, and be cautious in drawing conclusions.
• Communicate effectively: Communicate your research findings clearly and effectively to your target audience. Use appropriate language, visuals, and formats to ensure that your findings are understood and valued.
• Collaborate and seek feedback : Collaborate with other researchers, experts, or stakeholders in your field. Seek feedback on your research design, methods, and findings to ensure that they are relevant, meaningful, and impactful.

About the author

Muhammad Hassan

Researcher, Academic Writer, Web developer

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