hypothesis biology definition simple

What is a scientific hypothesis?

It's the initial building block in the scientific method.

Hypothesis basics

What makes a hypothesis testable.

• Types of hypotheses
• Hypothesis versus theory

Additional resources

Bibliography.

A scientific hypothesis is a tentative, testable explanation for a phenomenon in the natural world. It's the initial building block in the scientific method . Many describe it as an "educated guess" based on prior knowledge and observation. While this is true, a hypothesis is more informed than a guess. While an "educated guess" suggests a random prediction based on a person's expertise, developing a hypothesis requires active observation and background research. 

The basic idea of a hypothesis is that there is no predetermined outcome. For a solution to be termed a scientific hypothesis, it has to be an idea that can be supported or refuted through carefully crafted experimentation or observation. This concept, called falsifiability and testability, was advanced in the mid-20th century by Austrian-British philosopher Karl Popper in his famous book "The Logic of Scientific Discovery" (Routledge, 1959).

A key function of a hypothesis is to derive predictions about the results of future experiments and then perform those experiments to see whether they support the predictions.

A hypothesis is usually written in the form of an if-then statement, which gives a possibility (if) and explains what may happen because of the possibility (then). The statement could also include "may," according to California State University, Bakersfield .

Here are some examples of hypothesis statements:

• If garlic repels fleas, then a dog that is given garlic every day will not get fleas.
• If sugar causes cavities, then people who eat a lot of candy may be more prone to cavities.
• If ultraviolet light can damage the eyes, then maybe this light can cause blindness.

A useful hypothesis should be testable and falsifiable. That means that it should be possible to prove it wrong. A theory that can't be proved wrong is nonscientific, according to Karl Popper's 1963 book " Conjectures and Refutations ."

An example of an untestable statement is, "Dogs are better than cats." That's because the definition of "better" is vague and subjective. However, an untestable statement can be reworded to make it testable. For example, the previous statement could be changed to this: "Owning a dog is associated with higher levels of physical fitness than owning a cat." With this statement, the researcher can take measures of physical fitness from dog and cat owners and compare the two.

Types of scientific hypotheses

In an experiment, researchers generally state their hypotheses in two ways. The null hypothesis predicts that there will be no relationship between the variables tested, or no difference between the experimental groups. The alternative hypothesis predicts the opposite: that there will be a difference between the experimental groups. This is usually the hypothesis scientists are most interested in, according to the University of Miami .

For example, a null hypothesis might state, "There will be no difference in the rate of muscle growth between people who take a protein supplement and people who don't." The alternative hypothesis would state, "There will be a difference in the rate of muscle growth between people who take a protein supplement and people who don't."

If the results of the experiment show a relationship between the variables, then the null hypothesis has been rejected in favor of the alternative hypothesis, according to the book " Research Methods in Psychology " (​​BCcampus, 2015). 

There are other ways to describe an alternative hypothesis. The alternative hypothesis above does not specify a direction of the effect, only that there will be a difference between the two groups. That type of prediction is called a two-tailed hypothesis. If a hypothesis specifies a certain direction — for example, that people who take a protein supplement will gain more muscle than people who don't — it is called a one-tailed hypothesis, according to William M. K. Trochim , a professor of Policy Analysis and Management at Cornell University.

Sometimes, errors take place during an experiment. These errors can happen in one of two ways. A type I error is when the null hypothesis is rejected when it is true. This is also known as a false positive. A type II error occurs when the null hypothesis is not rejected when it is false. This is also known as a false negative, according to the University of California, Berkeley . 

A hypothesis can be rejected or modified, but it can never be proved correct 100% of the time. For example, a scientist can form a hypothesis stating that if a certain type of tomato has a gene for red pigment, that type of tomato will be red. During research, the scientist then finds that each tomato of this type is red. Though the findings confirm the hypothesis, there may be a tomato of that type somewhere in the world that isn't red. Thus, the hypothesis is true, but it may not be true 100% of the time.

Scientific theory vs. scientific hypothesis

The best hypotheses are simple. They deal with a relatively narrow set of phenomena. But theories are broader; they generally combine multiple hypotheses into a general explanation for a wide range of phenomena, according to the University of California, Berkeley . For example, a hypothesis might state, "If animals adapt to suit their environments, then birds that live on islands with lots of seeds to eat will have differently shaped beaks than birds that live on islands with lots of insects to eat." After testing many hypotheses like these, Charles Darwin formulated an overarching theory: the theory of evolution by natural selection.

"Theories are the ways that we make sense of what we observe in the natural world," Tanner said. "Theories are structures of ideas that explain and interpret facts." 

• Read more about writing a hypothesis, from the American Medical Writers Association.
• Find out why a hypothesis isn't always necessary in science, from The American Biology Teacher.
• Learn about null and alternative hypotheses, from Prof. Essa on YouTube .

Encyclopedia Britannica. Scientific Hypothesis. Jan. 13, 2022. https://www.britannica.com/science/scientific-hypothesis

Karl Popper, "The Logic of Scientific Discovery," Routledge, 1959.

California State University, Bakersfield, "Formatting a testable hypothesis." https://www.csub.edu/~ddodenhoff/Bio100/Bio100sp04/formattingahypothesis.htm  

Karl Popper, "Conjectures and Refutations," Routledge, 1963.

Price, P., Jhangiani, R., & Chiang, I., "Research Methods of Psychology — 2nd Canadian Edition," BCcampus, 2015.‌

University of Miami, "The Scientific Method" http://www.bio.miami.edu/dana/161/evolution/161app1_scimethod.pdf  

William M.K. Trochim, "Research Methods Knowledge Base," https://conjointly.com/kb/hypotheses-explained/  

University of California, Berkeley, "Multiple Hypothesis Testing and False Discovery Rate" https://www.stat.berkeley.edu/~hhuang/STAT141/Lecture-FDR.pdf  

University of California, Berkeley, "Science at multiple levels" https://undsci.berkeley.edu/article/0_0_0/howscienceworks_19

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What Is a Hypothesis? (Science)

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A hypothesis (plural hypotheses) is a proposed explanation for an observation. The definition depends on the subject.

In science, a hypothesis is part of the scientific method. It is a prediction or explanation that is tested by an experiment. Observations and experiments may disprove a scientific hypothesis, but can never entirely prove one.

In the study of logic, a hypothesis is an if-then proposition, typically written in the form, "If X , then Y ."

In common usage, a hypothesis is simply a proposed explanation or prediction, which may or may not be tested.

Writing a Hypothesis

Most scientific hypotheses are proposed in the if-then format because it's easy to design an experiment to see whether or not a cause and effect relationship exists between the independent variable and the dependent variable . The hypothesis is written as a prediction of the outcome of the experiment.

• Null Hypothesis and Alternative Hypothesis

Statistically, it's easier to show there is no relationship between two variables than to support their connection. So, scientists often propose the null hypothesis . The null hypothesis assumes changing the independent variable will have no effect on the dependent variable.

In contrast, the alternative hypothesis suggests changing the independent variable will have an effect on the dependent variable. Designing an experiment to test this hypothesis can be trickier because there are many ways to state an alternative hypothesis.

For example, consider a possible relationship between getting a good night's sleep and getting good grades. The null hypothesis might be stated: "The number of hours of sleep students get is unrelated to their grades" or "There is no correlation between hours of sleep and grades."

An experiment to test this hypothesis might involve collecting data, recording average hours of sleep for each student and grades. If a student who gets eight hours of sleep generally does better than students who get four hours of sleep or 10 hours of sleep, the hypothesis might be rejected.

But the alternative hypothesis is harder to propose and test. The most general statement would be: "The amount of sleep students get affects their grades." The hypothesis might also be stated as "If you get more sleep, your grades will improve" or "Students who get nine hours of sleep have better grades than those who get more or less sleep."

In an experiment, you can collect the same data, but the statistical analysis is less likely to give you a high confidence limit.

Usually, a scientist starts out with the null hypothesis. From there, it may be possible to propose and test an alternative hypothesis, to narrow down the relationship between the variables.

Example of a Hypothesis

Examples of a hypothesis include:

• If you drop a rock and a feather, (then) they will fall at the same rate.
• Plants need sunlight in order to live. (if sunlight, then life)
• Eating sugar gives you energy. (if sugar, then energy)
• White, Jay D.  Research in Public Administration . Conn., 1998.
• Schick, Theodore, and Lewis Vaughn.  How to Think about Weird Things: Critical Thinking for a New Age . McGraw-Hill Higher Education, 2002.
• Null Hypothesis Definition and Examples
• Definition of a Hypothesis
• What Are the Elements of a Good Hypothesis?
• Six Steps of the Scientific Method
• Independent Variable Definition and Examples
• What Are Examples of a Hypothesis?
• Understanding Simple vs Controlled Experiments
• Scientific Method Flow Chart
• Scientific Method Vocabulary Terms
• What Is a Testable Hypothesis?
• Null Hypothesis Examples
• What 'Fail to Reject' Means in a Hypothesis Test
• How To Design a Science Fair Experiment
• What Is an Experiment? Definition and Design
• Hypothesis Test for the Difference of Two Population Proportions

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High school biology

Course: high school biology   >   unit 1.

• Biology overview
• Preparing to study biology
• What is life?
• The scientific method
• Data to justify experimental claims examples
• Scientific method and data analysis
• Introduction to experimental design
• Controlled experiments

Biology and the scientific method review

• Experimental design and bias

The nature of biology

Properties of life.

• Organization: Living things are highly organized (meaning they contain specialized, coordinated parts) and are made up of one or more cells .
• Metabolism: Living things must use energy and consume nutrients to carry out the chemical reactions that sustain life. The sum total of the biochemical reactions occurring in an organism is called its metabolism .
• Homeostasis : Living organisms regulate their internal environment to maintain the relatively narrow range of conditions needed for cell function.
• Growth : Living organisms undergo regulated growth. Individual cells become larger in size, and multicellular organisms accumulate many cells through cell division.
• Reproduction : Living organisms can reproduce themselves to create new organisms.
• Response : Living organisms respond to stimuli or changes in their environment.
• Evolution : Populations of living organisms can undergo evolution , meaning that the genetic makeup of a population may change over time.

Scientific methodology

Scientific method example: failure to toast.

• Observation: the toaster won't toast.
• Question: Why won't my toaster toast?
• Hypothesis: Maybe the outlet is broken.
• Prediction: If I plug the toaster into a different outlet, then it will toast the bread.
• Test of prediction: Plug the toaster into a different outlet and try again.
• Iteration time!

Experimental design

Reducing errors and bias.

• Having a large sample size in the experiment: This helps to account for any small differences among the test subjects that may provide unexpected results.
• Repeating experimental trials multiple times: Errors may result from slight differences in test subjects, or mistakes in methodology or data collection. Repeating trials helps reduce those effects.
• Including all data points: Sometimes it is tempting to throw away data points that are inconsistent with the proposed hypothesis. However, this makes for an inaccurate study! All data points need to be included, whether they support the hypothesis or not.
• Using placebos , when appropriate: Placebos prevent the test subjects from knowing whether they received a real therapeutic substance. This helps researchers determine whether a substance has a true effect.
• Implementing double-blind studies , when appropriate: Double-blind studies prevent researchers from knowing the status of a particular participant. This helps eliminate observer bias.

Communicating findings

Things to remember.

• A hypothesis is not necessarily the right explanation. Instead, it is a possible explanation that can be tested to see if it is likely correct, or if a new hypothesis needs to be made.
• Not all explanations can be considered a hypothesis. A hypothesis must be testable and falsifiable in order to be valid. For example, “The universe is beautiful" is not a good hypothesis, because there is no experiment that could test this statement and show it to be false.
• In most cases, the scientific method is an iterative process. In other words, it's a cycle rather than a straight line. The result of one experiment often becomes feedback that raises questions for more experimentation.
• Scientists use the word "theory" in a very different way than non-scientists. When many people say "I have a theory," they really mean "I have a guess." Scientific theories, on the other hand, are well-tested and highly reliable scientific explanations of natural phenomena. They unify many repeated observations and data collected from lots of experiments.

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Module 1: Introduction to Biology

Experiments and hypotheses, learning outcomes.

• Form a hypothesis and use it to design a scientific experiment

Now we’ll focus on the methods of scientific inquiry. Science often involves making observations and developing hypotheses. Experiments and further observations are often used to test the hypotheses.

A scientific experiment is a carefully organized procedure in which the scientist intervenes in a system to change something, then observes the result of the change. Scientific inquiry often involves doing experiments, though not always. For example, a scientist studying the mating behaviors of ladybugs might begin with detailed observations of ladybugs mating in their natural habitats. While this research may not be experimental, it is scientific: it involves careful and verifiable observation of the natural world. The same scientist might then treat some of the ladybugs with a hormone hypothesized to trigger mating and observe whether these ladybugs mated sooner or more often than untreated ones. This would qualify as an experiment because the scientist is now making a change in the system and observing the effects.

Forming a Hypothesis

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis through observations and research, and it must be possible to prove your hypothesis false.

For example, Michael observes that maple trees lose their leaves in the fall. He might then propose a possible explanation for this observation: “cold weather causes maple trees to lose their leaves in the fall.” This statement is testable. He could grow maple trees in a warm enclosed environment such as a greenhouse and see if their leaves still dropped in the fall. The hypothesis is also falsifiable. If the leaves still dropped in the warm environment, then clearly temperature was not the main factor in causing maple leaves to drop in autumn.

In the Try It below, you can practice recognizing scientific hypotheses. As you consider each statement, try to think as a scientist would: can I test this hypothesis with observations or experiments? Is the statement falsifiable? If the answer to either of these questions is “no,” the statement is not a valid scientific hypothesis.

Practice Questions

Determine whether each following statement is a scientific hypothesis.

Air pollution from automobile exhaust can trigger symptoms in people with asthma.

• No. This statement is not testable or falsifiable.
• No. This statement is not testable.
• No. This statement is not falsifiable.
• Yes. This statement is testable and falsifiable.

Natural disasters, such as tornadoes, are punishments for bad thoughts and behaviors.

a: No. This statement is not testable or falsifiable. “Bad thoughts and behaviors” are excessively vague and subjective variables that would be impossible to measure or agree upon in a reliable way. The statement might be “falsifiable” if you came up with a counterexample: a “wicked” place that was not punished by a natural disaster. But some would question whether the people in that place were really wicked, and others would continue to predict that a natural disaster was bound to strike that place at some point. There is no reason to suspect that people’s immoral behavior affects the weather unless you bring up the intervention of a supernatural being, making this idea even harder to test.

Testing a Vaccine

Let’s examine the scientific process by discussing an actual scientific experiment conducted by researchers at the University of Washington. These researchers investigated whether a vaccine may reduce the incidence of the human papillomavirus (HPV). The experimental process and results were published in an article titled, “ A controlled trial of a human papillomavirus type 16 vaccine .”

Preliminary observations made by the researchers who conducted the HPV experiment are listed below:

• Human papillomavirus (HPV) is the most common sexually transmitted virus in the United States.
• There are about 40 different types of HPV. A significant number of people that have HPV are unaware of it because many of these viruses cause no symptoms.
• Some types of HPV can cause cervical cancer.
• About 4,000 women a year die of cervical cancer in the United States.

Practice Question

Researchers have developed a potential vaccine against HPV and want to test it. What is the first testable hypothesis that the researchers should study?

• HPV causes cervical cancer.
• People should not have unprotected sex with many partners.
• People who get the vaccine will not get HPV.
• The HPV vaccine will protect people against cancer.

Experimental Design

You’ve successfully identified a hypothesis for the University of Washington’s study on HPV: People who get the HPV vaccine will not get HPV.

The next step is to design an experiment that will test this hypothesis. There are several important factors to consider when designing a scientific experiment. First, scientific experiments must have an experimental group. This is the group that receives the experimental treatment necessary to address the hypothesis.

The experimental group receives the vaccine, but how can we know if the vaccine made a difference? Many things may change HPV infection rates in a group of people over time. To clearly show that the vaccine was effective in helping the experimental group, we need to include in our study an otherwise similar control group that does not get the treatment. We can then compare the two groups and determine if the vaccine made a difference. The control group shows us what happens in the absence of the factor under study.

However, the control group cannot get “nothing.” Instead, the control group often receives a placebo. A placebo is a procedure that has no expected therapeutic effect—such as giving a person a sugar pill or a shot containing only plain saline solution with no drug. Scientific studies have shown that the “placebo effect” can alter experimental results because when individuals are told that they are or are not being treated, this knowledge can alter their actions or their emotions, which can then alter the results of the experiment.

Moreover, if the doctor knows which group a patient is in, this can also influence the results of the experiment. Without saying so directly, the doctor may show—through body language or other subtle cues—their views about whether the patient is likely to get well. These errors can then alter the patient’s experience and change the results of the experiment. Therefore, many clinical studies are “double blind.” In these studies, neither the doctor nor the patient knows which group the patient is in until all experimental results have been collected.

Both placebo treatments and double-blind procedures are designed to prevent bias. Bias is any systematic error that makes a particular experimental outcome more or less likely. Errors can happen in any experiment: people make mistakes in measurement, instruments fail, computer glitches can alter data. But most such errors are random and don’t favor one outcome over another. Patients’ belief in a treatment can make it more likely to appear to “work.” Placebos and double-blind procedures are used to level the playing field so that both groups of study subjects are treated equally and share similar beliefs about their treatment.

The scientists who are researching the effectiveness of the HPV vaccine will test their hypothesis by separating 2,392 young women into two groups: the control group and the experimental group. Answer the following questions about these two groups.

• This group is given a placebo.
• This group is deliberately infected with HPV.
• This group is given nothing.
• This group is given the HPV vaccine.
• a: This group is given a placebo. A placebo will be a shot, just like the HPV vaccine, but it will have no active ingredient. It may change peoples’ thinking or behavior to have such a shot given to them, but it will not stimulate the immune systems of the subjects in the same way as predicted for the vaccine itself.
• d: This group is given the HPV vaccine. The experimental group will receive the HPV vaccine and researchers will then be able to see if it works, when compared to the control group.

Experimental Variables

A variable is a characteristic of a subject (in this case, of a person in the study) that can vary over time or among individuals. Sometimes a variable takes the form of a category, such as male or female; often a variable can be measured precisely, such as body height. Ideally, only one variable is different between the control group and the experimental group in a scientific experiment. Otherwise, the researchers will not be able to determine which variable caused any differences seen in the results. For example, imagine that the people in the control group were, on average, much more sexually active than the people in the experimental group. If, at the end of the experiment, the control group had a higher rate of HPV infection, could you confidently determine why? Maybe the experimental subjects were protected by the vaccine, but maybe they were protected by their low level of sexual contact.

To avoid this situation, experimenters make sure that their subject groups are as similar as possible in all variables except for the variable that is being tested in the experiment. This variable, or factor, will be deliberately changed in the experimental group. The one variable that is different between the two groups is called the independent variable. An independent variable is known or hypothesized to cause some outcome. Imagine an educational researcher investigating the effectiveness of a new teaching strategy in a classroom. The experimental group receives the new teaching strategy, while the control group receives the traditional strategy. It is the teaching strategy that is the independent variable in this scenario. In an experiment, the independent variable is the variable that the scientist deliberately changes or imposes on the subjects.

Dependent variables are known or hypothesized consequences; they are the effects that result from changes or differences in an independent variable. In an experiment, the dependent variables are those that the scientist measures before, during, and particularly at the end of the experiment to see if they have changed as expected. The dependent variable must be stated so that it is clear how it will be observed or measured. Rather than comparing “learning” among students (which is a vague and difficult to measure concept), an educational researcher might choose to compare test scores, which are very specific and easy to measure.

In any real-world example, many, many variables MIGHT affect the outcome of an experiment, yet only one or a few independent variables can be tested. Other variables must be kept as similar as possible between the study groups and are called control variables . For our educational research example, if the control group consisted only of people between the ages of 18 and 20 and the experimental group contained people between the ages of 30 and 35, we would not know if it was the teaching strategy or the students’ ages that played a larger role in the results. To avoid this problem, a good study will be set up so that each group contains students with a similar age profile. In a well-designed educational research study, student age will be a controlled variable, along with other possibly important factors like gender, past educational achievement, and pre-existing knowledge of the subject area.

What is the independent variable in this experiment?

• Sex (all of the subjects will be female)
• Presence or absence of the HPV vaccine
• Presence or absence of HPV (the virus)

List three control variables other than age.

What is the dependent variable in this experiment?

• Sex (male or female)
• Rates of HPV infection
• Age (years)
• Revision and adaptation. Authored by : Shelli Carter and Lumen Learning. Provided by : Lumen Learning. License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike
• Scientific Inquiry. Provided by : Open Learning Initiative. Located at : https://oli.cmu.edu/jcourse/workbook/activity/page?context=434a5c2680020ca6017c03488572e0f8 . Project : Introduction to Biology (Open + Free). License : CC BY-NC-SA: Attribution-NonCommercial-ShareAlike

1.2 The Process of Science

Learning objectives.

• Identify the shared characteristics of the natural sciences
• Understand the process of scientific inquiry
• Compare inductive reasoning with deductive reasoning
• Describe the goals of basic science and applied science

Like geology, physics, and chemistry, biology is a science that gathers knowledge about the natural world. Specifically, biology is the study of life. The discoveries of biology are made by a community of researchers who work individually and together using agreed-on methods. In this sense, biology, like all sciences is a social enterprise like politics or the arts. The methods of science include careful observation, record keeping, logical and mathematical reasoning, experimentation, and submitting conclusions to the scrutiny of others. Science also requires considerable imagination and creativity; a well-designed experiment is commonly described as elegant, or beautiful. Like politics, science has considerable practical implications and some science is dedicated to practical applications, such as the prevention of disease (see Figure 1.15 ). Other science proceeds largely motivated by curiosity. Whatever its goal, there is no doubt that science, including biology, has transformed human existence and will continue to do so.

The Nature of Science

Biology is a science, but what exactly is science? What does the study of biology share with other scientific disciplines? Science (from the Latin scientia, meaning "knowledge") can be defined as knowledge about the natural world.

Science is a very specific way of learning, or knowing, about the world. The history of the past 500 years demonstrates that science is a very powerful way of knowing about the world; it is largely responsible for the technological revolutions that have taken place during this time. There are however, areas of knowledge and human experience that the methods of science cannot be applied to. These include such things as answering purely moral questions, aesthetic questions, or what can be generally categorized as spiritual questions. Science cannot investigate these areas because they are outside the realm of material phenomena, the phenomena of matter and energy, and cannot be observed and measured.

The scientific method is a method of research with defined steps that include experiments and careful observation. The steps of the scientific method will be examined in detail later, but one of the most important aspects of this method is the testing of hypotheses. A hypothesis is a suggested explanation for an event, which can be tested. Hypotheses, or tentative explanations, are generally produced within the context of a scientific theory . A generally accepted scientific theory is thoroughly tested and confirmed explanation for a set of observations or phenomena. Scientific theory is the foundation of scientific knowledge. In addition, in many scientific disciplines (less so in biology) there are scientific laws , often expressed in mathematical formulas, which describe how elements of nature will behave under certain specific conditions. There is not an evolution of hypotheses through theories to laws as if they represented some increase in certainty about the world. Hypotheses are the day-to-day material that scientists work with and they are developed within the context of theories. Laws are concise descriptions of parts of the world that are amenable to formulaic or mathematical description.

Natural Sciences

What would you expect to see in a museum of natural sciences? Frogs? Plants? Dinosaur skeletons? Exhibits about how the brain functions? A planetarium? Gems and minerals? Or maybe all of the above? Science includes such diverse fields as astronomy, biology, computer sciences, geology, logic, physics, chemistry, and mathematics ( Figure 1.16 ). However, those fields of science related to the physical world and its phenomena and processes are considered natural sciences . Thus, a museum of natural sciences might contain any of the items listed above.

There is no complete agreement when it comes to defining what the natural sciences include. For some experts, the natural sciences are astronomy, biology, chemistry, earth science, and physics. Other scholars choose to divide natural sciences into life sciences , which study living things and include biology, and physical sciences , which study nonliving matter and include astronomy, physics, and chemistry. Some disciplines such as biophysics and biochemistry build on two sciences and are interdisciplinary.

Scientific Inquiry

One thing is common to all forms of science: an ultimate goal “to know.” Curiosity and inquiry are the driving forces for the development of science. Scientists seek to understand the world and the way it operates. Two methods of logical thinking are used: inductive reasoning and deductive reasoning.

Inductive reasoning is a form of logical thinking that uses related observations to arrive at a general conclusion. This type of reasoning is common in descriptive science. A life scientist such as a biologist makes observations and records them. These data can be qualitative (descriptive) or quantitative (consisting of numbers), and the raw data can be supplemented with drawings, pictures, photos, or videos. From many observations, the scientist can infer conclusions (inductions) based on evidence. Inductive reasoning involves formulating generalizations inferred from careful observation and the analysis of a large amount of data. Brain studies often work this way. Many brains are observed while people are doing a task. The part of the brain that lights up, indicating activity, is then demonstrated to be the part controlling the response to that task.

Deductive reasoning or deduction is the type of logic used in hypothesis-based science. In deductive reasoning, the pattern of thinking moves in the opposite direction as compared to inductive reasoning. Deductive reasoning is a form of logical thinking that uses a general principle or law to predict specific results. From those general principles, a scientist can deduce and predict the specific results that would be valid as long as the general principles are valid. For example, a prediction would be that if the climate is becoming warmer in a region, the distribution of plants and animals should change. Comparisons have been made between distributions in the past and the present, and the many changes that have been found are consistent with a warming climate. Finding the change in distribution is evidence that the climate change conclusion is a valid one.

Both types of logical thinking are related to the two main pathways of scientific study: descriptive science and hypothesis-based science. Descriptive (or discovery) science aims to observe, explore, and discover, while hypothesis-based science begins with a specific question or problem and a potential answer or solution that can be tested. The boundary between these two forms of study is often blurred, because most scientific endeavors combine both approaches. Observations lead to questions, questions lead to forming a hypothesis as a possible answer to those questions, and then the hypothesis is tested. Thus, descriptive science and hypothesis-based science are in continuous dialogue.

Hypothesis Testing

Biologists study the living world by posing questions about it and seeking science-based responses. This approach is common to other sciences as well and is often referred to as the scientific method. The scientific method was used even in ancient times, but it was first documented by England’s Sir Francis Bacon (1561–1626) ( Figure 1.17 ), who set up inductive methods for scientific inquiry. The scientific method is not exclusively used by biologists but can be applied to almost anything as a logical problem-solving method.

The scientific process typically starts with an observation (often a problem to be solved) that leads to a question. Let’s think about a simple problem that starts with an observation and apply the scientific method to solve the problem. One Monday morning, a student arrives at class and quickly discovers that the classroom is too warm. That is an observation that also describes a problem: the classroom is too warm. The student then asks a question: “Why is the classroom so warm?”

Recall that a hypothesis is a suggested explanation that can be tested. To solve a problem, several hypotheses may be proposed. For example, one hypothesis might be, “The classroom is warm because no one turned on the air conditioning.” But there could be other responses to the question, and therefore other hypotheses may be proposed. A second hypothesis might be, “The classroom is warm because there is a power failure, and so the air conditioning doesn’t work.”

Once a hypothesis has been selected, a prediction may be made. A prediction is similar to a hypothesis but it typically has the format “If . . . then . . . .” For example, the prediction for the first hypothesis might be, “ If the student turns on the air conditioning, then the classroom will no longer be too warm.”

A hypothesis must be testable to ensure that it is valid. For example, a hypothesis that depends on what a bear thinks is not testable, because it can never be known what a bear thinks. It should also be falsifiable , meaning that it can be disproven by experimental results. An example of an unfalsifiable hypothesis is “Botticelli’s Birth of Venus is beautiful.” There is no experiment that might show this statement to be false. To test a hypothesis, a researcher will conduct one or more experiments designed to eliminate one or more of the hypotheses. This is important. A hypothesis can be disproven, or eliminated, but it can never be proven. Science does not deal in proofs like mathematics. If an experiment fails to disprove a hypothesis, then we find support for that explanation, but this is not to say that down the road a better explanation will not be found, or a more carefully designed experiment will be found to falsify the hypothesis.

Each experiment will have one or more variables and one or more controls. A variable is any part of the experiment that can vary or change during the experiment. A control is a part of the experiment that does not change. Look for the variables and controls in the example that follows. As a simple example, an experiment might be conducted to test the hypothesis that phosphate limits the growth of algae in freshwater ponds. A series of artificial ponds are filled with water and half of them are treated by adding phosphate each week, while the other half are treated by adding a salt that is known not to be used by algae. The variable here is the phosphate (or lack of phosphate), the experimental or treatment cases are the ponds with added phosphate and the control ponds are those with something inert added, such as the salt. Just adding something is also a control against the possibility that adding extra matter to the pond has an effect. If the treated ponds show lesser growth of algae, then we have found support for our hypothesis. If they do not, then we reject our hypothesis. Be aware that rejecting one hypothesis does not determine whether or not the other hypotheses can be accepted; it simply eliminates one hypothesis that is not valid ( Figure 1.18 ). Using the scientific method, the hypotheses that are inconsistent with experimental data are rejected.

In recent years a new approach of testing hypotheses has developed as a result of an exponential growth of data deposited in various databases. Using computer algorithms and statistical analyses of data in databases, a new field of so-called "data research" (also referred to as "in silico" research) provides new methods of data analyses and their interpretation. This will increase the demand for specialists in both biology and computer science, a promising career opportunity.

Visual Connection

In the example below, the scientific method is used to solve an everyday problem. Which part in the example below is the hypothesis? Which is the prediction? Based on the results of the experiment, is the hypothesis supported? If it is not supported, propose some alternative hypotheses.

• My toaster doesn’t toast my bread.
• Why doesn’t my toaster work?
• There is something wrong with the electrical outlet.
• If something is wrong with the outlet, my coffeemaker also won’t work when plugged into it.
• I plug my coffeemaker into the outlet.
• My coffeemaker works.

In practice, the scientific method is not as rigid and structured as it might at first appear. Sometimes an experiment leads to conclusions that favor a change in approach; often, an experiment brings entirely new scientific questions to the puzzle. Many times, science does not operate in a linear fashion; instead, scientists continually draw inferences and make generalizations, finding patterns as their research proceeds. Scientific reasoning is more complex than the scientific method alone suggests.

Basic and Applied Science

The scientific community has been debating for the last few decades about the value of different types of science. Is it valuable to pursue science for the sake of simply gaining knowledge, or does scientific knowledge only have worth if we can apply it to solving a specific problem or bettering our lives? This question focuses on the differences between two types of science: basic science and applied science.

Basic science or “pure” science seeks to expand knowledge regardless of the short-term application of that knowledge. It is not focused on developing a product or a service of immediate public or commercial value. The immediate goal of basic science is knowledge for knowledge’s sake, though this does not mean that in the end it may not result in an application.

In contrast, applied science or “technology,” aims to use science to solve real-world problems, making it possible, for example, to improve a crop yield, find a cure for a particular disease, or save animals threatened by a natural disaster. In applied science, the problem is usually defined for the researcher.

Some individuals may perceive applied science as “useful” and basic science as “useless.” A question these people might pose to a scientist advocating knowledge acquisition would be, “What for?” A careful look at the history of science, however, reveals that basic knowledge has resulted in many remarkable applications of great value. Many scientists think that a basic understanding of science is necessary before an application is developed; therefore, applied science relies on the results generated through basic science. Other scientists think that it is time to move on from basic science and instead to find solutions to actual problems. Both approaches are valid. It is true that there are problems that demand immediate attention; however, few solutions would be found without the help of the knowledge generated through basic science.

One example of how basic and applied science can work together to solve practical problems occurred after the discovery of DNA structure led to an understanding of the molecular mechanisms governing DNA replication. Strands of DNA, unique in every human, are found in our cells, where they provide the instructions necessary for life. During DNA replication, new copies of DNA are made, shortly before a cell divides to form new cells. Understanding the mechanisms of DNA replication enabled scientists to develop laboratory techniques that are now used to identify genetic diseases, pinpoint individuals who were at a crime scene, and determine paternity. Without basic science, it is unlikely that applied science could exist.

Another example of the link between basic and applied research is the Human Genome Project, a study in which each human chromosome was analyzed and mapped to determine the precise sequence of DNA subunits and the exact location of each gene. (The gene is the basic unit of heredity represented by a specific DNA segment that codes for a functional molecule.) Other organisms have also been studied as part of this project to gain a better understanding of human chromosomes. The Human Genome Project ( Figure 1.19 ) relied on basic research carried out with non-human organisms and, later, with the human genome. An important end goal eventually became using the data for applied research seeking cures for genetically related diseases.

While research efforts in both basic science and applied science are usually carefully planned, it is important to note that some discoveries are made by serendipity, that is, by means of a fortunate accident or a lucky surprise. Penicillin was discovered when biologist Alexander Fleming accidentally left a petri dish of Staphylococcus bacteria open. An unwanted mold grew, killing the bacteria. The mold turned out to be Penicillium , and a new critically important antibiotic was discovered. In a similar manner, Percy Lavon Julian was an established medicinal chemist working on a way to mass produce compounds with which to manufacture important drugs. He was focused on using soybean oil in the production of progesterone (a hormone important in the menstrual cycle and pregnancy), but it wasn't until water accidentally leaked into a large soybean oil storage tank that he found his method. Immediately recognizing the resulting substance as stigmasterol, a primary ingredient in progesterone and similar drugs, he began the process of replicating and industrializing the process in a manner that has helped millions of people. Even in the highly organized world of science, luck—when combined with an observant, curious mind focused on the types of reasoning discussed above—can lead to unexpected breakthroughs.

Reporting Scientific Work

Whether scientific research is basic science or applied science, scientists must share their findings for other researchers to expand and build upon their discoveries. Communication and collaboration within and between sub disciplines of science are key to the advancement of knowledge in science. For this reason, an important aspect of a scientist’s work is disseminating results and communicating with peers. Scientists can share results by presenting them at a scientific meeting or conference, but this approach can reach only the limited few who are present. Instead, most scientists present their results in peer-reviewed articles that are published in scientific journals. Peer-reviewed articles are scientific papers that are reviewed, usually anonymously by a scientist’s colleagues, or peers. These colleagues are qualified individuals, often experts in the same research area, who judge whether or not the scientist’s work is suitable for publication. The process of peer review helps to ensure that the research described in a scientific paper or grant proposal is original, significant, logical, and thorough. Grant proposals, which are requests for research funding, are also subject to peer review. Scientists publish their work so other scientists can reproduce their experiments under similar or different conditions to expand on the findings.

There are many journals and the popular press that do not use a peer-review system. A large number of online open-access journals, journals with articles available without cost, are now available many of which use rigorous peer-review systems, but some of which do not. Results of any studies published in these forums without peer review are not reliable and should not form the basis for other scientific work. In one exception, journals may allow a researcher to cite a personal communication from another researcher about unpublished results with the cited author’s permission.

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Definition of hypothesis

Did you know.

The Difference Between Hypothesis and Theory

A hypothesis is an assumption, an idea that is proposed for the sake of argument so that it can be tested to see if it might be true.

In the scientific method, the hypothesis is constructed before any applicable research has been done, apart from a basic background review. You ask a question, read up on what has been studied before, and then form a hypothesis.

A hypothesis is usually tentative; it's an assumption or suggestion made strictly for the objective of being tested.

A theory , in contrast, is a principle that has been formed as an attempt to explain things that have already been substantiated by data. It is used in the names of a number of principles accepted in the scientific community, such as the Big Bang Theory . Because of the rigors of experimentation and control, it is understood to be more likely to be true than a hypothesis is.

In non-scientific use, however, hypothesis and theory are often used interchangeably to mean simply an idea, speculation, or hunch, with theory being the more common choice.

Since this casual use does away with the distinctions upheld by the scientific community, hypothesis and theory are prone to being wrongly interpreted even when they are encountered in scientific contexts—or at least, contexts that allude to scientific study without making the critical distinction that scientists employ when weighing hypotheses and theories.

The most common occurrence is when theory is interpreted—and sometimes even gleefully seized upon—to mean something having less truth value than other scientific principles. (The word law applies to principles so firmly established that they are almost never questioned, such as the law of gravity.)

This mistake is one of projection: since we use theory in general to mean something lightly speculated, then it's implied that scientists must be talking about the same level of uncertainty when they use theory to refer to their well-tested and reasoned principles.

The distinction has come to the forefront particularly on occasions when the content of science curricula in schools has been challenged—notably, when a school board in Georgia put stickers on textbooks stating that evolution was "a theory, not a fact, regarding the origin of living things." As Kenneth R. Miller, a cell biologist at Brown University, has said , a theory "doesn’t mean a hunch or a guess. A theory is a system of explanations that ties together a whole bunch of facts. It not only explains those facts, but predicts what you ought to find from other observations and experiments.”

While theories are never completely infallible, they form the basis of scientific reasoning because, as Miller said "to the best of our ability, we’ve tested them, and they’ve held up."

• proposition
• supposition

hypothesis , theory , law mean a formula derived by inference from scientific data that explains a principle operating in nature.

hypothesis implies insufficient evidence to provide more than a tentative explanation.

theory implies a greater range of evidence and greater likelihood of truth.

law implies a statement of order and relation in nature that has been found to be invariable under the same conditions.

Examples of hypothesis in a Sentence

These examples are programmatically compiled from various online sources to illustrate current usage of the word 'hypothesis.' Any opinions expressed in the examples do not represent those of Merriam-Webster or its editors. Send us feedback about these examples.

Word History

Greek, from hypotithenai to put under, suppose, from hypo- + tithenai to put — more at do

1641, in the meaning defined at sense 1a

Phrases Containing hypothesis

• counter - hypothesis
• nebular hypothesis
• null hypothesis
• planetesimal hypothesis
• Whorfian hypothesis

Articles Related to hypothesis

This is the Difference Between a...

This is the Difference Between a Hypothesis and a Theory

In scientific reasoning, they're two completely different things

Dictionary Entries Near hypothesis

hypothermia

hypothesize

Cite this Entry

“Hypothesis.” Merriam-Webster.com Dictionary , Merriam-Webster, https://www.merriam-webster.com/dictionary/hypothesis. Accessed 8 May. 2024.

Kids Definition

Kids definition of hypothesis, medical definition, medical definition of hypothesis, more from merriam-webster on hypothesis.

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Britannica.com: Encyclopedia article about hypothesis

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Scientific Theory Definition and Examples

A scientific theory is a well-established explanation of some aspect of the natural world. Theories come from scientific data and multiple experiments. While it is not possible to prove a theory, a single contrary result using the scientific method can disprove it. In other words, a theory is testable and falsifiable.

Examples of Scientific Theories

There are many scientific theory in different disciplines:

• Astronomy : theory of stellar nucleosynthesis , theory of stellar evolution
• Biology : cell theory, theory of evolution, germ theory, dual inheritance theory
• Chemistry : atomic theory, Bronsted Lowry acid-base theory , kinetic molecular theory of gases , Lewis acid-base theory , molecular theory, valence bond theory
• Geology : climate change theory, plate tectonics theory
• Physics : Big Bang theory, perturbation theory, theory of relativity, quantum field theory

Criteria for a Theory

In order for an explanation of the natural world to be a theory, it meets certain criteria:

• A theory is falsifiable. At some point, a theory withstands testing and experimentation using the scientific method.
• A theory is supported by lots of independent evidence.
• A theory explains existing experimental results and predicts outcomes of new experiments at least as well as other theories.

Difference Between a Scientific Theory and Theory

Usually, a scientific theory is just called a theory. However, a theory in science means something different from the way most people use the word. For example, if frogs rain down from the sky, a person might observe the frogs and say, “I have a theory about why that happened.” While that theory might be an explanation, it is not based on multiple observations and experiments. It might not be testable and falsifiable. It’s not a scientific theory (although it could eventually become one).

Value of Disproven Theories

Even though some theories are incorrect, they often retain value.

For example, Arrhenius acid-base theory does not explain the behavior of chemicals lacking hydrogen that behave as acids. The Bronsted Lowry and Lewis theories do a better job of explaining this behavior. Yet, the Arrhenius theory predicts the behavior of most acids and is easier for people to understand.

Another example is the theory of Newtonian mechanics. The theory of relativity is much more inclusive than Newtonian mechanics, which breaks down in certain frames of reference or at speeds close to the speed of light . But, Newtonian mechanics is much simpler to understand and its equations apply to everyday behavior.

Difference Between a Scientific Theory and a Scientific Law

The scientific method leads to the formulation of both scientific theories and laws . Both theories and laws are falsifiable. Both theories and laws help with making predictions about the natural world. However, there is a key difference.

A theory explains why or how something works, while a law describes what happens without explaining it. Often, you see laws written in the form of equations or formulas.

Theories and laws are related, but theories never become laws or vice versa.

Theory vs Hypothesis

A hypothesis is a proposition that is tested via an experiment. A theory results from many, many tested hypotheses.

Theory vs Fact

Theories depend on facts, but the two words mean different things. A fact is an irrefutable piece of evidence or data. Facts never change. A theory, on the other hand, may be modified or disproven.

Difference Between a Theory and a Model

Both theories and models allow a scientist to form a hypothesis and make predictions about future outcomes. However, a theory both describes and explains, while a model only describes. For example, a model of the solar system shows the arrangement of planets and asteroids in a plane around the Sun, but it does not explain how or why they got into their positions.

• Frigg, Roman (2006). “ Scientific Representation and the Semantic View of Theories .”  Theoria . 55 (2): 183–206. 
• Halvorson, Hans (2012). “What Scientific Theories Could Not Be.”  Philosophy of Science . 79 (2): 183–206. doi: 10.1086/664745
• McComas, William F. (December 30, 2013).  The Language of Science Education: An Expanded Glossary of Key Terms and Concepts in Science Teaching and Learning . Springer Science & Business Media. ISBN 978-94-6209-497-0.
• National Academy of Sciences (US) (1999). Science and Creationism: A View from the National Academy of Sciences (2nd ed.). National Academies Press. doi: 10.17226/6024  ISBN 978-0-309-06406-4. 
• Suppe, Frederick (1998). “Understanding Scientific Theories: An Assessment of Developments, 1969–1998.”  Philosophy of Science . 67: S102–S115. doi: 10.1086/392812

Related Posts

Null hypothesis

Null hypothesis n., plural: null hypotheses [nʌl haɪˈpɒθɪsɪs] Definition: a hypothesis that is valid or presumed true until invalidated by a statistical test

Table of Contents

Null Hypothesis Definition

Null hypothesis is defined as “the commonly accepted fact (such as the sky is blue) and researcher aim to reject or nullify this fact”.

More formally, we can define a null hypothesis as “a statistical theory suggesting that no statistical relationship exists between given observed variables” .

In biology , the null hypothesis is used to nullify or reject a common belief. The researcher carries out the research which is aimed at rejecting the commonly accepted belief.

What Is a Null Hypothesis?

A hypothesis is defined as a theory or an assumption that is based on inadequate evidence. It needs and requires more experiments and testing for confirmation. There are two possibilities that by doing more experiments and testing, a hypothesis can be false or true. It means it can either prove wrong or true (Blackwelder, 1982).

For example, Susie assumes that mineral water helps in the better growth and nourishment of plants over distilled water. To prove this hypothesis, she performs this experiment for almost a month. She watered some plants with mineral water and some with distilled water.

In a hypothesis when there are no statistically significant relationships among the two variables, the hypothesis is said to be a null hypothesis. The investigator is trying to disprove such a hypothesis. In the above example of plants, the null hypothesis is:

There are no statistical relationships among the forms of water that are given to plants for growth and nourishment.

Usually, an investigator tries to prove the null hypothesis wrong and tries to explain a relation and association between the two variables.

An opposite and reverse of the null hypothesis are known as the alternate hypothesis . In the example of plants the alternate hypothesis is:

There are statistical relationships among the forms of water that are given to plants for growth and nourishment.

The example below shows the difference between null vs alternative hypotheses:

Alternate Hypothesis: The world is round Null Hypothesis: The world is not round.

Copernicus and many other scientists try to prove the null hypothesis wrong and false. By their experiments and testing, they make people believe that alternate hypotheses are correct and true. If they do not prove the null hypothesis experimentally wrong then people will not believe them and never consider the alternative hypothesis true and correct.

The alternative and null hypothesis for Susie’s assumption is:

• Null Hypothesis: If one plant is watered with distilled water and the other with mineral water, then there is no difference in the growth and nourishment of these two plants.
• Alternative Hypothesis:  If one plant is watered with distilled water and the other with mineral water, then the plant with mineral water shows better growth and nourishment.

The null hypothesis suggests that there is no significant or statistical relationship. The relation can either be in a single set of variables or among two sets of variables.

Most people consider the null hypothesis true and correct. Scientists work and perform different experiments and do a variety of research so that they can prove the null hypothesis wrong or nullify it. For this purpose, they design an alternate hypothesis that they think is correct or true. The null hypothesis symbol is H 0 (it is read as H null or H zero ).

Why is it named the “Null”?

The name null is given to this hypothesis to clarify and explain that the scientists are working to prove it false i.e. to nullify the hypothesis. Sometimes it confuses the readers; they might misunderstand it and think that statement has nothing. It is blank but, actually, it is not. It is more appropriate and suitable to call it a nullifiable hypothesis instead of the null hypothesis.

Why do we need to assess it? Why not just verify an alternate one?

In science, the scientific method is used. It involves a series of different steps. Scientists perform these steps so that a hypothesis can be proved false or true. Scientists do this to confirm that there will be any limitation or inadequacy in the new hypothesis. Experiments are done by considering both alternative and null hypotheses, which makes the research safe. It gives a negative as well as a bad impact on research if a null hypothesis is not included or a part of the study. It seems like you are not taking your research seriously and not concerned about it and just want to impose your results as correct and true if the null hypothesis is not a part of the study.

Development of the Null

In statistics, firstly it is necessary to design alternate and null hypotheses from the given problem. Splitting the problem into small steps makes the pathway towards the solution easier and less challenging. how to write a null hypothesis?

Writing a null hypothesis consists of two steps:

• Firstly, initiate by asking a question.
• Secondly, restate the question in such a way that it seems there are no relationships among the variables.

In other words, assume in such a way that the treatment does not have any effect.

The usual recovery duration after knee surgery is considered almost 8 weeks.

A researcher thinks that the recovery period may get elongated if patients go to a physiotherapist for rehabilitation twice per week, instead of thrice per week, i.e. recovery duration reduces if the patient goes three times for rehabilitation instead of two times.

Step 1: Look for the problem in the hypothesis. The hypothesis either be a word or can be a statement. In the above example the hypothesis is:

“The expected recovery period in knee rehabilitation is more than 8 weeks”

Step 2: Make a mathematical statement from the hypothesis. Averages can also be represented as μ, thus the null hypothesis formula will be.

In the above equation, the hypothesis is equivalent to H1, the average is denoted by μ and > that the average is greater than eight.

Step 3: Explain what will come up if the hypothesis does not come right i.e., the rehabilitation period may not proceed more than 08 weeks.

There are two options: either the recovery will be less than or equal to 8 weeks.

H 0 : μ ≤ 8

In the above equation, the null hypothesis is equivalent to H 0 , the average is denoted by μ and ≤ represents that the average is less than or equal to eight.

What will happen if the scientist does not have any knowledge about the outcome?

Problem: An investigator investigates the post-operative impact and influence of radical exercise on patients who have operative procedures of the knee. The chances are either the exercise will improve the recovery or will make it worse. The usual time for recovery is 8 weeks.

Step 1: Make a null hypothesis i.e. the exercise does not show any effect and the recovery time remains almost 8 weeks.

H 0 : μ = 8

In the above equation, the null hypothesis is equivalent to H 0 , the average is denoted by μ, and the equal sign (=) shows that the average is equal to eight.

Step 2: Make the alternate hypothesis which is the reverse of the null hypothesis. Particularly what will happen if treatment (exercise) makes an impact?

In the above equation, the alternate hypothesis is equivalent to H1, the average is denoted by μ and not equal sign (≠) represents that the average is not equal to eight.

Significance Tests

To get a reasonable and probable clarification of statistics (data), a significance test is performed. The null hypothesis does not have data. It is a piece of information or statement which contains numerical figures about the population. The data can be in different forms like in means or proportions. It can either be the difference of proportions and means or any odd ratio.

The following table will explain the symbols:

P-value is the chief statistical final result of the significance test of the null hypothesis.

• P-value = Pr(data or data more extreme | H 0 true)
• | = “given”
• Pr = probability
• H 0 = the null hypothesis

The first stage of Null Hypothesis Significance Testing (NHST) is to form an alternate and null hypothesis. By this, the research question can be briefly explained.

Null Hypothesis = no effect of treatment, no difference, no association Alternative Hypothesis = effective treatment, difference, association

When to reject the null hypothesis?

Researchers will reject the null hypothesis if it is proven wrong after experimentation. Researchers accept null hypothesis to be true and correct until it is proven wrong or false. On the other hand, the researchers try to strengthen the alternate hypothesis. The binomial test is performed on a sample and after that, a series of tests were performed (Frick, 1995).

Step 1: Evaluate and read the research question carefully and consciously and make a null hypothesis. Verify the sample that supports the binomial proportion. If there is no difference then find out the value of the binomial parameter.

Show the null hypothesis as:

H 0 :p= the value of p if H 0 is true

To find out how much it varies from the proposed data and the value of the null hypothesis, calculate the sample proportion.

Step 2: In test statistics, find the binomial test that comes under the null hypothesis. The test must be based on precise and thorough probabilities. Also make a list of pmf that apply, when the null hypothesis proves true and correct.

When H 0 is true, X~b(n, p)

N = size of the sample

P = assume value if H 0 proves true.

Step 3: Find out the value of P. P-value is the probability of data that is under observation.

Rise or increase in the P value = Pr(X ≥ x)

X = observed number of successes

P value = Pr(X ≤ x).

Step 4: Demonstrate the findings or outcomes in a descriptive detailed way.

• Sample proportion
• The direction of difference (either increases or decreases)

Perceived Problems With the Null Hypothesis

Variable or model selection and less information in some cases are the chief important issues that affect the testing of the null hypothesis. Statistical tests of the null hypothesis are reasonably not strong. There is randomization about significance. (Gill, 1999) The main issue with the testing of the null hypothesis is that they all are wrong or false on a ground basis.

There is another problem with the a-level . This is an ignored but also a well-known problem. The value of a-level is without a theoretical basis and thus there is randomization in conventional values, most commonly 0.q, 0.5, or 0.01. If a fixed value of a is used, it will result in the formation of two categories (significant and non-significant) The issue of a randomized rejection or non-rejection is also present when there is a practical matter which is the strong point of the evidence related to a scientific matter.

The P-value has the foremost importance in the testing of null hypothesis but as an inferential tool and for interpretation, it has a problem. The P-value is the probability of getting a test statistic at least as extreme as the observed one.

The main point about the definition is: Observed results are not based on a-value

Moreover, the evidence against the null hypothesis was overstated due to unobserved results. A-value has importance more than just being a statement. It is a precise statement about the evidence from the observed results or data. Similarly, researchers found that P-values are objectionable. They do not prefer null hypotheses in testing. It is also clear that the P-value is strictly dependent on the null hypothesis. It is computer-based statistics. In some precise experiments, the null hypothesis statistics and actual sampling distribution are closely related but this does not become possible in observational studies.

Some researchers pointed out that the P-value is depending on the sample size. If the true and exact difference is small, a null hypothesis even of a large sample may get rejected. This shows the difference between biological importance and statistical significance. (Killeen, 2005)

Another issue is the fix a-level, i.e., 0.1. On the basis, if a-level a null hypothesis of a large sample may get accepted or rejected. If the size of simple is infinity and the null hypothesis is proved true there are still chances of Type I error. That is the reason this approach or method is not considered consistent and reliable. There is also another problem that the exact information about the precision and size of the estimated effect cannot be known. The only solution is to state the size of the effect and its precision.

Null Hypothesis Examples

Here are some examples:

Example 1: Hypotheses with One Sample of One Categorical Variable

Among all the population of humans, almost 10% of people prefer to do their task with their left hand i.e. left-handed. Let suppose, a researcher in the Penn States says that the population of students at the College of Arts and Architecture is mostly left-handed as compared to the general population of humans in general public society. In this case, there is only a sample and there is a comparison among the known population values to the population proportion of sample value.

• Research Question: Do artists more expected to be left-handed as compared to the common population persons in society?
• Response Variable: Sorting the student into two categories. One category has left-handed persons and the other category have right-handed persons.
• Form Null Hypothesis: Arts and Architecture college students are no more predicted to be lefty as compared to the common population persons in society (Lefty students of Arts and Architecture college population is 10% or p= 0.10)

Example 2: Hypotheses with One Sample of One Measurement Variable

A generic brand of antihistamine Diphenhydramine making medicine in the form of a capsule, having a 50mg dose. The maker of the medicines is concerned that the machine has come out of calibration and is not making more capsules with the suitable and appropriate dose.

• Research Question: Does the statistical data recommended about the mean and average dosage of the population differ from 50mg?
• Response Variable: Chemical assay used to find the appropriate dosage of the active ingredient.
• Null Hypothesis: Usually, the 50mg dosage of capsules of this trade name (population average and means dosage =50 mg).

Example 3: Hypotheses with Two Samples of One Categorical Variable

Several people choose vegetarian meals on a daily basis. Typically, the researcher thought that females like vegetarian meals more than males.

• Research Question: Does the data recommend that females (women) prefer vegetarian meals more than males (men) regularly?
• Response Variable: Cataloguing the persons into vegetarian and non-vegetarian categories. Grouping Variable: Gender
• Null Hypothesis: Gender is not linked to those who like vegetarian meals. (Population percent of women who eat vegetarian meals regularly = population percent of men who eat vegetarian meals regularly or p women = p men).

Example 4: Hypotheses with Two Samples of One Measurement Variable

Nowadays obesity and being overweight is one of the major and dangerous health issues. Research is performed to confirm that a low carbohydrates diet leads to faster weight loss than a low-fat diet.

• Research Question: Does the given data recommend that usually, a low-carbohydrate diet helps in losing weight faster as compared to a low-fat diet?
• Response Variable: Weight loss (pounds)
• Explanatory Variable: Form of diet either low carbohydrate or low fat
• Null Hypothesis: There is no significant difference when comparing the mean loss of weight of people using a low carbohydrate diet to people using a diet having low fat. (population means loss of weight on a low carbohydrate diet = population means loss of weight on a diet containing low fat).

Example 5: Hypotheses about the relationship between Two Categorical Variables

A case-control study was performed. The study contains nonsmokers, stroke patients, and controls. The subjects are of the same occupation and age and the question was asked if someone at their home or close surrounding smokes?

• Research Question: Did second-hand smoke enhance the chances of stroke?
• Variables: There are 02 diverse categories of variables. (Controls and stroke patients) (whether the smoker lives in the same house). The chances of having a stroke will be increased if a person is living with a smoker.
• Null Hypothesis: There is no significant relationship between a passive smoker and stroke or brain attack. (odds ratio between stroke and the passive smoker is equal to 1).

Example 6: Hypotheses about the relationship between Two Measurement Variables

A financial expert observes that there is somehow a positive and effective relationship between the variation in stock rate price and the quantity of stock bought by non-management employees

• Response variable- Regular alteration in price
• Explanatory Variable- Stock bought by non-management employees
• Null Hypothesis: The association and relationship between the regular stock price alteration ($) and the daily stock-buying by non-management employees ($) = 0.

Example 7: Hypotheses about comparing the relationship between Two Measurement Variables in Two Samples

• Research Question: Is the relation between the bill paid in a restaurant and the tip given to the waiter, is linear? Is this relation different for dining and family restaurants?
• Explanatory Variable- total bill amount
• Response Variable- the amount of tip
• Null Hypothesis: The relationship and association between the total bill quantity at a family or dining restaurant and the tip, is the same.

Try to answer the quiz below to check what you have learned so far about the null hypothesis.

Choose the best answer. 

Send Your Results (Optional)

• Blackwelder, W. C. (1982). “Proving the null hypothesis” in clinical trials. Controlled Clinical Trials , 3(4), 345–353.
• Frick, R. W. (1995). Accepting the null hypothesis. Memory & Cognition, 23(1), 132–138.
• Gill, J. (1999). The insignificance of null hypothesis significance testing. Political Research Quarterly , 52(3), 647–674.
• Killeen, P. R. (2005). An alternative to null-hypothesis significance tests. Psychological Science, 16(5), 345–353.

©BiologyOnline.com. Content provided and moderated by Biology Online Editors.

Last updated on June 16th, 2022

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1.3: Scientific Theories

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As you view Know the Difference (Between Hypothesis and Theory) , focus on these concepts: the controversy surrounding the words ‘‘hypothesis’’ and ‘‘theory’’, the scientific use of the words ‘‘hypothesis’’ and ‘‘theory’’, the criteria for a ‘‘hypothesis,’’ the National Academy of Sciences definition of ‘‘theory’’, the meaning of the statement, ‘‘theories are the bedrock of our understanding of nature’’.

The Theory of Evolution

The theory of evolution by natural selection is a scientific theory. Evolution is a change in the characteristics of living things over time. Evolution occurs by a process called natural selection . In natural selection, some living things produce more offspring than others, so they pass more genes to the next generation than others do. Over many generations, this can lead to major changes in the characteristics of living things. The theory of evolution by natural selection explains how living things are changing today and how modern living things have descended from ancient life forms that no longer exist on Earth. No evidence has been identified that proves this theory is incorrect. More on the theory of evolution will be presented in additional concepts.

The Cell Theory

The cell theory is another important scientific theory of biology. According to the cell theory , the cell is the smallest unit of structure and function of all living organisms, all living organisms are made up of at least one cell, and living cells always come from other living cells. Once again, no evidence has been identified that proves this theory is incorrect. More on the cell theory will be presented in additional concepts.

The Germ Theory

The germ theory of disease, also called the pathogenic theory of medicine, is a scientific theory that proposes that microorganisms are the cause of many diseases. Like the other scientific theories, lots of evidence has been identified that supports this theory, and no evidence has been identified that proves the theory is incorrect.

• With repeated testing, some hypotheses may eventually become scientific theories. A scientific theory is a broad explanation for events that is widely accepted as true.
• Evolution is a change species over time. Evolution occurs by natural selection.
• The cell theory states that all living things are made up of cells, and living cells always come from other living cells.
• The germ theory proposes that microorganisms are the cause of many diseases.

Explore More

Use these resources to answer the questions that follow.

Explore More I

• Darwinian Evolution - Science and Theory at Non-Majors Biology: http://www.hippocampus.org/Biology .
• How is the word ‘‘theory’’ used in common language?
• How is the word ‘‘theory’’ used in science?
• Provide a detailed definition for a ‘‘scientific theory’’.

Explore More II

• Concepts and Methods in Biology - Theories and Laws at Non-Majors Biology: http://www.hippocampus.org/Biology .
• What is a scientific law ?
• What is a scientific theory?
• Give two examples of scientific theories.
• Can a scientific theory become a law? Why or why not?
• Contrast how the term theory is used in science and in everyday language.
• Explain how a hypothesis could become a theory.
• Describe the evidence that proves the cell theory is incorrect.