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Biotechnology articles from across Nature Portfolio
Biotechnology is a broad discipline in which biological processes, organisms, cells or cellular components are exploited to develop new technologies. New tools and products developed by biotechnologists are useful in research, agriculture, industry and the clinic.
Co-evolved genes improve the biosynthesis of secondary metabolites
A new engineering strategy for improving the biosynthesis of secondary metabolites in Streptomyces has been developed through the analysis of genes co-evolved with biosynthetic gene clusters. This strategy has been verified in 11 Streptomyces strains to enhance production of 16,385 metabolites, showing potential applications in drug discovery and industrial production.
Genetically encoding colors and images into bioengineered microbial materials
Using synthetic biology, we engineered a cellulose-producing bacterium that can produce eumelanin and respond to light, so that it is possible to grow a microbial leather material that is colored black or contains projected black patterns.
Affinity maturation of CRISPR-engineered B cell receptors in vivo
CRISPR–Cas12a was used to directly replace mouse antibody variable chain genes with human versions in primary B cells. The edited cells underwent affinity maturation in vivo, improving the potency of HIV-1 and SARS-CoV-2 neutralizing antibodies without loss of bioavailability. Affinity maturation of edited cells also enables new vaccine models and adaptive B cell therapies.
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Latest Research and Reviews
Nose-to-brain selective drug delivery to glioma via ferritin-based nanovectors reduces tumor growth and improves survival rate
- Francesco Marrocco
- Elisabetta Falvo
- Gianni Colotti
A modular and synthetic biosynthesis platform for de novo production of diverse halogenated tryptophan-derived molecules
De novo fermentation and synthetic pathway construction for halogen-containing molecules remain relatively underexplored. Here, the authors report a mix-and-match co-culture platform to de novo generate a large array of halogenated tryptophan derivatives in E. coli from glucose.
- Kevin B. Reed
- Sierra M. Brooks
- Hal S. Alper
Elucidation of genes enhancing natural product biosynthesis through co-evolution analysis
The authors develop a metabolic engineering strategy for improving polyketide production of industrial interest and discovering new natural products in bacteria.
- Xinran Wang
- Ningxin Chen
- Xiaozhou Luo
Metabotropic glutamate receptor genetic variants and peripheral receptor expression affects trait scores of autistic probands
- Nilanjana Dutta
- Mahasweta Chatterjee
- Kanchan Mukhopadhyay
Pseudomonas putida as saviour for troubled Synechococcus elongatus in a synthetic co-culture – interaction studies based on a multi-OMICs approach
This study investigates interspecies interaction in a synthetic co-culture consisting of the sucrose-secreting cyanobacterium S. elongatus cscB and its sucrose-consuming partner P. putida cscRABY by employing a comprehensive multi-OMICs approach.
- Franziska Kratzl
- Marlene Urban
- Katharina Pflüger-Grau
A bone adhesive enhances osteoporotic fracture repair by regulating bone homeostasis
Schematic diagrams of GORoM adhesive and osteoporotic fractures treatment . A The process of OVX rat model establishment and GORoM adhesive implantation. B Osteoporotic fracture healing was mediated by the osteogenesis promotion and osteoclast inhibition.
- Shenghui Su
News and Comment
SIMPLE-seq to decode DNA methylation dynamics in single cells
In this Tools of the Trade article, Dongsheng Bai and Chenxu Zhu describe SIMPLE-seq, a scalable single-cell sequencing method that simultaneously decodes the cytosine modifications 5mC and 5hmC.
- Dongsheng Bai
Response to the Commentary on: Safety and feasibility of percutaneous needle tunneling with platelet-rich plasma injections for Peyronie’s disease in the outpatient setting: a pilot study
- Ahmed S. Zugail
- Muaath Alshuaibi
- Sébastien Beley
‘Mini liver’ will grow in person’s own lymph node in bold new trial
Biotechnology firm LyGenesis has injected donor cells into a person with liver failure for the first time.
Descemet’s membrane or confirmation of a novel corneal structure: the layer of Dua
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Page 1 of 35
Transcriptomic and targeted metabolomic analyses provide insights into the flavonoids biosynthesis in the flowers of Lonicera macranthoides
Flavonoids are one of the bioactive ingredients of Lonicera macranthoides ( L. macranthoides ), however, their biosynthesis in the flower is still unclear. In this study, combined transcriptomic and targeted metabo...
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Effects of solid lipid nanocarrier containing methyl urolithin A by coating folate-bound chitosan and evaluation of its anti-cancer activity
Nanotechnology-based drug delivery systems have received much attention over the past decade. In the present study, we synthesized Methyl Urolithin A-loaded solid lipid nanoparticles decorated with the folic a...
Neq2X7: a multi-purpose and open-source fusion DNA polymerase for advanced DNA engineering and diagnostics PCR
Thermostable DNA polymerases, such as Taq isolated from the thermophilic bacterium Thermus aquaticus , enable one-pot exponential DNA amplification known as polymerase chain reaction (PCR). However, properties oth...
A solution for highly efficient electroporation of primary cytotoxic T lymphocytes
Cytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research depend on efficient and reliable transfection. Although various metho...
Adsorption of Hg 2+ /Cr 6+ by metal-binding proteins heterologously expressed in Escherichia coli
Removal of heavy metals from water and soil is a pressing challenge in environmental engineering, and biosorption by microorganisms is considered as one of the most cost-effective methods. In this study, the m...
Derivation of a novel antimicrobial peptide from the Red Sea Brine Pools modified to enhance its anticancer activity against U2OS cells
Cancer associated drug resistance is a major cause for cancer aggravation, particularly as conventional therapies have presented limited efficiency, low specificity, resulting in long term deleterious side eff...
Polyphyllin B inhibited STAT3/NCOA4 pathway and restored gut microbiota to ameliorate lung tissue injury in cigarette smoke-induced mice
Smoking was a major risk factor for chronic obstructive pulmonary disease (COPD). This study plan to explore the mechanism of Polyphyllin B in lung injury induced by cigarette smoke (CSE) in COPD.
Quantifying carboxymethyl lysine and carboxyethyl lysine in human plasma: clinical insights into aging research using liquid chromatography-tandem mass spectrometry
The objective of this study was to establish a methodology for determining carboxymethyl lysine (CML) and carboxyethyl lysine (CEL) concentrations in human plasma using liquid chromatography-tandem mass spectr...
Iron/Copper/Phosphate nanocomposite as antimicrobial, antisnail, and wheat growth-promoting agent
One of the current challenges is to secure wheat crop production to meet the increasing global food demand and to face the increase in its purchasing power. Therefore, the current study aimed to exploit a new ...
Staphopain mediated virulence and antibiotic resistance alteration in co-infection of Staphylococcus aureus and Pseudomonas aeruginosa : an animal model
Polymicrobial communities lead to worsen the wound infections, due to mixed biofilms, increased antibiotic resistance, and altered virulence production. Promising approaches, including enzymes, may overcome th...
Strain-specific features of Pleurotus ostreatus growth in vitro and some of its biological activities
The production of Pleurotus ostreatus mycelium as a promising object for use in food and other industries is hampered by a lack of information about the strain-specificity of this fungus mycelium growth and its a...
Antibacterial, antibiofilm, and anticancer activity of silver-nanoparticles synthesized from the cell-filtrate of Streptomyces enissocaesilis
Silver nanoparticles (Ag-NPs) have a unique mode of action as antibacterial agents in addition to their anticancer and antioxidant properties. In this study, microbial nanotechnology is employed to synthesize ...
Deep orange gene editing triggers temperature-sensitive lethal phenotypes in Ceratitis capitata
The Mediterranean fruit fly, Ceratitis capitata , is a significant agricultural pest managed through area-wide integrated pest management (AW-IPM) including a sterile insect technique (SIT) component. Male-only re...
Characterization, modeling, and anticancer activity of L.arginase production from marine Bacillus licheniformis OF2
L-arginase, is a powerful anticancer that hydrolyzes L-arginine to L-ornithine and urea. This enzyme is widely distributed and expressed in organisms like plants, fungi, however very scarce from bacteria. Our ...
Green and environmentally friendly synthesis of silver nanoparticles with antibacterial properties from some medicinal plants
Recently there have been a variety of methods to synthesize silver nanoparticles, among which the biosynthesis method is more noticeable due to features like being eco-friendly, simple, and cost-efficient. The...
Reaping the benefits of liquid handlers for high-throughput gene expression profiling in a marine model invertebrate
Modern high-throughput technologies enable the processing of a large number of samples simultaneously, while also providing rapid and accurate procedures. In recent years, automated liquid handling workstation...
Induction of antimicrobial, antioxidant metabolites production by co-cultivation of two red-sea-sponge-associated Aspergillus sp. CO2 and Bacillus sp. COBZ21
The growing spread of infectious diseases has become a potential global health threat to human beings. According to WHO reports, in this study, we investigated the impact of co-cultivating the isolated endophy...
A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing
Lytic polysaccharide monooxygenases (LPMOs) catalyzing the oxidative cleavage of different types of polysaccharides have potential to be used in various industries. However, AA13 family LPMOs which specificall...
Tuning spacer length improves the functionality of the nanobody-based VEGFR2 CAR T cell
The chimeric antigen receptor-expressing T (CAR-T) cells for cancer immunotherapy have obtained considerable clinical importance. CAR T cells need an optimized intracellular signaling domain to get appropriate...
Fabrication and characterization of metformin-loaded PLGA/Collagen nanofibers for modulation of macrophage polarization for tissue engineering and regenerative medicine
In tissue engineering (TE) and regenerative medicine, the accessibility of engineered scaffolds that modulate inflammatory states is extremely necessary. The aim of the current work was to assess the efficacy ...
Production of a potential multistrain probiotic in co-culture conditions using agro-industrial by-products-based medium for fish nutrition
Probiotics are viable microorganisms that when administered in adequate amounts confer health benefits to the host. In fish, probiotic administration has improved growth, and immunological parameters. For this...
Research on the targeted improvement of the yield of a new VB 12 -producing strain, Ensifer adhaerens S305, based on genomic and transcriptomic analysis
Vitamin B 12 (VB 12 ) has a wide range of applications and high economic value. In this study, a new strain with high VB 12 production potential, Ensifer adhaerens S305, was identified in sewage. Because E. adhaerens
Validation and calibration of a novel GEM biosensor for specific detection of Cd 2+ , Zn 2+ , and Pb 2+
In this study, we designed a novel genetic circuit sensitive to Cd 2+ , Zn 2+ and Pb 2+ by mimicking the CadA/CadR operon system mediated heavy metal homeostasis mechanism of Pseudomonas aeruginosa . The regular DNA m...
Exploring the microbial diversity and characterization of cellulase and hemicellulase genes in goat rumen: a metagenomic approach
Goat rumen microbial communities are perceived as one of the most potential biochemical reservoirs of multi-functional enzymes, which are applicable to enhance wide array of bioprocesses such as the hydrolysis...
The transcriptional factor Clr-5 is involved in cellulose degradation through regulation of amino acid metabolism in Neurospora crassa
Filamentous fungi are efficient degraders of plant biomass and the primary producers of commercial cellulolytic enzymes. While the transcriptional regulation mechanisms of cellulases have been continuously exp...
An online soft sensor method for biochemical reaction process based on JS-ISSA-XGBoost
A method combining offline techniques and the just-in-time learning strategy (JITL) is proposed, because the biochemical reaction process often encounters changing features and parameters over time.
Preparation, purification, and biochemical of fat-degrading bacterial enzymes from pig carcass compost and its application
A lot of kitchen waste oil is produced every day worldwide, leading to serious environmental pollution. As one of the environmental protection methods, microorganisms are widely used treating of various wastes...
Coupling fermentation of glutamic acid and γ-polyglutamic acid and preparation of poly(amino acid) superabsorbent polymers
γ-polyglutamic acid (γ-PGA) is a biomarker that can be directly obtained by microbial fermentation. Poly(amino acid) superabsorbent polymers (SAPs) were prepared with purified γ-PGA as raw material and ethylen...
Serodiagnosis of human brucellosis by an indirect ELISA test using recombinant outer membrane protein 19 kDa (rOMP19) as an antigen
Brucellosis remains one of the global health concerns that reemerges in recent years. Delayed or inaccurate diagnosis end to a long treatment duration and financial burden; therefore, finding a good antigen fo...
A magnetic solid phase chemiluminescent immunoassay for quantification of Cystatin C in human serum
A chemiluminescent immunoassay for human serum Cystatin C (Cys C) was established using a direct-antibody sandwich model. The immunoassay kit uses magnetic separation technology, using magnetic particles as th...
Target identification of small molecules: an overview of the current applications in drug discovery
Target identification is an essential part of the drug discovery and development process, and its efficacy plays a crucial role in the success of any given therapy. Although protein target identification resea...
Engineered FGF19 ΔKLB protects against intrahepatic cholestatic liver injury in ANIT-induced and Mdr2-/- mice model
The major safety concern of the clinical application of wild type FGF19 (FGF19 WT ) emerges given that its extended treatment causes hepatocellular carcinoma. Therefore, we previously generated a safer FGF19 varian...
Use of immunoinformatics and the simulation approach to identify Helicobacter pylori epitopes to design a multi-epitope subunit vaccine for B- and T-cells
Helicobacter pylori cause a variety of gastric malignancies, gastric ulcers, and cause erosive diseases. The extreme nature of the bacterium and the implantation of this bacterium protects it against designing a ...
Antifungal screening of selenium nanoparticles biosynthesized by microcystin-producing Desmonostoc alborizicum
Metal nanoparticles exhibit excellent antifungal abilities and are seen as a good substitute for controlling different kinds of fungi. Of all known taxa, cyanobacteria have received significant consideration a...
Expanding the genetic toolbox for Cutaneotrichosporon oleaginosus employing newly identified promoters and a novel antibiotic resistance marker
Cutaneotrichosporon oleaginosus is an oleaginous yeast that can produce up to 80% lipid per dry weight. Its high capacity for the biosynthesis of single cell oil makes it highly interesting for the production of ...
Effect of poly (lactic-co-glycolic acid) polymer nanoparticles loaded with vancomycin against Staphylococcus aureus biofilm
Staphylococcus aureus is a unique challenge for the healthcare system because it can form biofilms, is resistant to the host's immune system, and is resistant to numerous antimicrobial therapies. The aim of this ...
A novel allogeneic acellular matrix scaffold for porcine cartilage regeneration
Cartilage defects are common sports injuries without significant treatment. Articular cartilage with inferior regenerative potential resulted in the poor formation of hyaline cartilage in defects. Acellular ma...
Development of short hairpin RNA expression vectors targeting the internal ribosomal entry site of the classical swine fever virus genomic RNA
Classical swine fever (CSF) is a fatal contagious disease affecting pigs caused by classical swine fever virus (CSFV). The disease can be transmitted by pigs and wild boars, and it is difficult to prevent and ...
Solubility of lamotrigine in age-specific biorelevant media that simulated the fasted- and fed-conditions of the gastric and intestinal environments in pediatrics and adults: implications for traditional, re-formulated, modified, and new oral formulations
Lamotrigine is an effective antiseizure medication that can be used in the management of focal and generalized epilepsies in pediatric patients. This study was conducted to quantify and compare the solubility ...
Comparison of the effects of nitrogen-, sulfur- and combined nitrogen- and sulfur-deprivations on cell growth, lipid bodies and gene expressions in Chlamydomonas reinhardtii cc5373-sta6
Biofuel research that aims to optimize growth conditions in microalgae is critically important. Chlamydomonas reinhardtii is a green microalga that offers advantages for biofuel production research. This study co...
Development of an itaconic acid production process with Ustilaginaceae on alternative feedstocks
Currently, Aspergillus terreus is used for the industrial production of itaconic acid. Although, alternative feedstock use in fermentations is crucial for cost-efficient and sustainable itaconic acid production, ...
Whole-cell catalyze L-dopa to dopamine via co-expression of transport protein AroP in Escherichia coli
Dopamine is high-value compound of pharmaceutical interest, but its industrial scale production mostly focuses on chemical synthesis, possessing environment pollution. Bio-manufacturing has caused much attenti...
An effective device to enable consistent scratches for in vitro scratch assays
The in-vitro scratch assay is a useful method in wound healing research to assess cell migration. In this assay, a scratch is created in a confluent cell layer by mechanically removing cells through manual scr...
Highly multiplexed targeted sequencing strategy for infectious disease surveillance
Global efforts to characterize diseases of poverty are hampered by lack of affordable and comprehensive detection platforms, resulting in suboptimal allocation of health care resources and inefficient disease ...
Determination of serum CA724 levels using fluorescence immunochromatography
Carbohydrate antigen 724 (CA724) is a sensitive and specific indicator for multiple malignant tumors. The aim of this study was to establish a Eu-time resolved fluorescence immunochromatography (Eu-TRFICO) met...
Efficient delivery of a large-size Cas9-EGFP vector in porcine fetal fibroblasts using a Lonza 4D-Nucleofector system
Porcine fetal fibroblasts (PFFs) are important donor cells for generating genetically modified pigs, but the transfection efficiencies of PFFs are often unsatisfactory especially when large-size vectors are to...
A nano-Liposomal formulation potentiates antioxidant, anti-inflammatory, and fibrinolytic activities of Allolobophora caliginosa coelomic fluid: formulation and characterization
Coelomic fluid, a pharmacologically active compound in earthworms, exhibits a range of biological activities, including antioxidant, anti-inflammatory, and anticancer. However, the biological activities exerte...
Prediction and optimization of indirect shoot regeneration of Passiflora caerulea using machine learning and optimization algorithms
Optimization of indirect shoot regeneration protocols is one of the key prerequisites for the development of Agrobacterium -mediated genetic transformation and/or genome editing in Passiflora caerulea . Comprehensi...
The impact of applying various de novo assembly and correction tools on the identification of genome characterization, drug resistance, and virulence factors of clinical isolates using ONT sequencing
Oxford Nanopore sequencing technology (ONT) is currently widely used due to its affordability, simplicity, and reliability. Despite the advantage ONT has over next-generation sequencing in detecting resistance...
Development of a mammalian cell-based ZZ display system for IgG quantification
Biological laboratories and companies involved in antibody development need convenient and versatile methods to detect highly active antibodies.
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The future is bright, the future is biotechnology
* E-mail: [email protected]
Affiliation Public Library of Science, San Francisco, California, United States of America and Cambridge, United Kingdom
- Richard Hodge,
- on behalf of the PLOS Biology staff editors
Published: April 28, 2023
- https://doi.org/10.1371/journal.pbio.3002135
- Reader Comments
As PLOS Biology celebrates its 20 th anniversary, our April issue focuses on biotechnology with articles covering different aspects of the field, from genome editing to synthetic biology. With them, we emphasize our interest in expanding our presence in biotechnology research.
Citation: Hodge R, on behalf of the PLOS Biology staff editors (2023) The future is bright, the future is biotechnology. PLoS Biol 21(4): e3002135. https://doi.org/10.1371/journal.pbio.3002135
Copyright: © 2023 Hodge, on behalf of the PLOS Biology staff editors. This is an open access article distributed under the terms of the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The authors received no specific funding for this work.
Competing interests: The authors have declared that no competing interests exist.
The PLOS Biology Staff Editors are Ines Alvarez-Garcia, Joanna Clarke, RichardHodge, Paula Jauregui, Nonia Pariente, Roland Roberts, and Lucas Smith.
This article is part of the PLOS Biology 20th Anniversary Collection.
Biotechnology is a revolutionary branch of science at the forefront of research and innovation that has advanced rapidly in recent years. It is a broad discipline, in which organisms or biological processes are exploited to develop new technologies that have the potential to transform the way we live and work, as well as to boost sustainability and industrial productivity. The new tools and products being generated have a wide range of applications across various sectors, including medicine, agriculture, energy, manufacturing and food.
PLOS Biology has traditionally published research reporting significant advances across a wide range of biological disciplines. However, our scope must continue to evolve as biology increasingly becomes more and more applied, generating technologies with potentially game-changing therapeutic and environmental impact. To that end, we recently published a collection of magazine articles focused on ideas for green biotechnologies that could have an important role in a sustainable future [ 1 ], including how to harness microbial photosynthesis to directly generate electricity [ 2 ] and using microbes to develop carbon “sinks” in the mining industry [ 3 ]. Moreover, throughout this anniversary year we are publishing Perspective articles that take stock of the past 20 years of biological research in a specific field and look forward to what is to come in the next 20 years [ 4 ]; in this issue, these Perspectives focus on different aspects of the broad biotechnology field—synthetic biology [ 5 ] and the use of lipid nanoparticles (LNPs) for the delivery of therapeutics [ 6 ].
One fast moving area within biotechnology is gene editing therapy, which involves the alteration of DNA to treat or prevent disease using techniques such as CRISPR-Cas9 and base editors that enable precise genetic modifications to be made. This approach shows great promise for treating a variety of genetic diseases. Excitingly, promising phase I results of the first in vivo genome editing clinical trial to treat several liver-related diseases were reported at the recent Keystone Symposium on Precision Genome Engineering. This issue of PLOS Biology includes an Essay from Porto and Komor that focuses on the clinical applications of base editor technology [ 7 ], which could enable chronic diseases to be treated with a ‘one-and-done’ therapy, and a Perspective from Hamilton and colleagues that outlines the advances in the development of LNPs for the delivery of nucleic acid-based therapeutics [ 6 ]. LNPs are commonly used as vehicles for the delivery of such therapeutics because they have a low immunogenicity and can be manufactured at scale. However, expanding the toolbox of delivery platforms for these novel therapeutics will be critical to realise their full clinical potential.
Synthetic biology is also a rapidly growing area, whereby artificial or existing biological systems are designed to produce products or enhance cellular function. By using CRISPR to edit genes involved in metabolic pathways, researchers can create organisms that produce valuable compounds such as biofuels, drugs, and industrial chemicals. In their Perspective, Kitano and colleagues take stock of the technological advances that have propelled the “design-build-test-learn” cycle methodology forward in synthetic biology, as well as focusing on how machine-learning approaches can remove the bottlenecks in these pipelines [ 5 ].
While the potential of these technologies is vast, there are also concerns about their safety and ethical implications. Gene editing, in particular, raises ethical concerns, as it could be used to create so-called “designer babies” with specific traits or to enhance physical or mental capabilities. There are also concerns about the unintended consequences of gene editing, such as off-target effects that could cause unintended harm. These technologies can be improved by better understanding the interplay between editing tools and DNA repair pathways, and it will be essential for scientists and policymakers to be cautious and work together to establish guidelines and regulations for their use, as outlined at the recent International Summit on Human Genome Editing .
Basic research has also benefitted from biotechnological developments. For instance, methodological developments in super-resolution microscopy offer researchers the ability to image cells at exquisite detail and answer previously inaccessible research questions. Sequencing technologies such as Nanopore sequencers are revolutionising the ability to sequence long DNA/RNA reads in real time and in the field. Great strides have also been made in the development of analysis software for structural biology purposes, such as sub-tomogram averaging for cryo-EM [ 8 ]. The rate of scientific discovery is now at an unprecedented level in this age of big data as a result of these huge technological leaps.
The past few years has also seen the launch of AI tools such as ChatGPT. While these tools are increasingly being used to help write students homework or to improve the text of scientific papers, generative AI tools hold the potential to transform research and development in the biotechnology industry. The recently developed language model ProGen can generate and then predict function in protein sequences [ 9 ], and these models can also be used to find therapeutically relevant compounds for drug discovery. Protein structure prediction programs, such as AlphaFold [ 10 ] and RosettaFold, have revolutionized structural biology and can be used for a myriad of purposes. We have recently published several papers that have utilized AlphaFold models to develop methods that determine the structural context of post-translational modifications [ 11 ] and predict autophagy-related motifs in proteins [ 12 ].
The future of biotechnology is clearly very promising and we look forward to being part of the dissemination of these important new developments. Open access science sits at the core of our mission and the publication of these novel technologies in PLOS Biology can help their widespread adoption and ensure global access. As we look forward during this year of celebration, we are excited that biotechnology research will continue to grow and become a central part of the journal. The future is bright and the future is very much biotechnology.
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- © 2021
Agricultural Biotechnology: Latest Research and Trends
- Dinesh Kumar Srivastava 0 ,
- Ajay Kumar Thakur 1 ,
- Pankaj Kumar 2
Department of Biotechnology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, India
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Biotechnology Division, ICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, India
- In-depth compilation on the latest global trends of plant tissue culture and transgenic crops
- Brings out focused content RNAi, miRNA and on development of marker-free transgenic plants
- Discusses naontechnology, genome engineering and plant - omics applications in agricultural practices
48k Accesses
40 Citations
19 Altmetric
- Table of contents
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Table of contents (30 chapters)
Front matter, commercial micropropagation of some economically important crops.
- Daksha Bhatt
Clonal Propagation, a Tested Technique for Increasing Productivity: A Review of Bamboos, Eucalyptus and Chirpine
- V. Kataria, A. Masih, S. Chauhan, S. K. Sharma, A. Kant, I. D. Arya
In Vitro Production of Medicinal Compounds from Endangered and Commercially Important Medicinal Plants
- Neha Sharma, Hemant Sood
Double Haploid Production and Its Applications in Crop Improvement
- Awadhesh Kumar Mishra, Rajesh Saini, Kavindra Nath Tiwari
Encapsulation Technology: An Assessment of Its Role in In Vitro Conservation of Medicinal and Threatened Plant Species
- Manoj K. Rai, Roshni Rathour, Shashikanta Behera, Sandeep Kaushik, Soumendra K. Naik
Somaclonal Variation in Improvement of Agricultural Crops: Recent Progress
- Manoj K. Rai
Genetic Fidelity Studies for Testing True-to-Type Plants in Some Horticultural and Medicinal Crops Using Molecular Markers
- Sapna Tyagi, Deepak Rajpurohit, Amit Sharma
Callus Culture Approach Towards Production of Plant Secondary Metabolites
- Shiv Rattan, Mahinder Partap, Ashrita, Kanika, Pankaj Kumar, Archit Sood et al.
Transgenic Implications for Biotic and Abiotic Stress Tolerance in Agricultural Crops
- Shabnam Sircaik, Karuna Dhiman, Geetika Gambhir, Pankaj Kumar, Dinesh Kumar Srivastava
Production of Marker-Free Transgenic Plants
- Urvashi Sharma, Ajinder Kaur, Jagdeep Singh Sandhu
Recent Progress in Cereals Biofortification to Alleviate Malnutrition in India: An Overview
- Pankaj Kumar, Arun Kumar, Karuna Dhiman, Dinesh Kumar Srivastava
Potential and Perspective of Plant Proteinase Inhibitor Genes in Genetic Improvement of Economically Important Crops
- Pawan S. Mainkar, Manju Sharma, Yamini Agarwal, Vijay K. Gupta, Rekha Kansal
Global Status of Genetically Modified Crops
- Vipasha Verma, Shivanti Negi, Pankaj Kumar, Dinesh Kumar Srivastava
Organic GMOs: Combining Ancient Wisdom with Modern Biotechnology
- Amjad M. Husaini
Genomics in Crop Improvement: Potential Applications, Challenges and Future Prospects
- Jeshima Khan Yasin, Masudulla Khan, Shabir H. Wani, M. Arumugam Pillai, Nidhi Verma, P. Pandey et al.
Proteomic Approaches to Understand Plant Response to Abiotic Stresses
- Ragini Sinha, Meenu Bala, Alok Ranjan, Shambhu Krishan Lal, Tilak Raj Sharma, Arunava Pattanayak et al.
Plant Metabolomics for Crop Improvement
- Rahul Narasanna, Aadil Mansoori, Neelam Mishra, Vinay Sharma, Sherinmol Thomas, Abhaypratap Vishwakarma et al.
New Generation Plant Phenomics Applications for Next Generation Agricultural Practices
- Aysen Yumurtaci, Hulya Sipahi
RNA Interference Technology as a Novel and Potential Alternative for Plant Improvement
- Ranjeet Kaur, Arundhati Ghosh, Manchikatla V. Rajam
This book caters to the need of researchers working in the ever-evolving field of agricultural biotechnology. It discusses and provides in-depth information about latest advancements happening in this field. The book discusses evolution of plant tissue culture techniques, development of doubled haploids technology, role of recombinant-DNA technology in crop improvement. It also provides an insight into the global status of genetically modified crops, use of RNAi technology and mi-RNAs in plant improvement. Chapters are also dedicated for different branches of ‘omics’ science including genomics, bioinformatics, proteomics, metabolomics and phenomics along with the use of molecular markers in tagging and mapping of various genes/QTLs of agronomic importance. This book also covers the role of enzymes and microbes in agriculture in productivity enhancement. It is of interest to teachers, researchers of biotechnology and agriculture scientists. Also the book serves as additional readingmaterial for undergraduate and postgraduate students of biotechnology, agriculture, horticulture, forestry, ecology, soil science, and environmental sciences. National and international biotechnologists and agricultural scientists will also find this to be a useful read.
- Biotechnology
- transgenic plants
- marker-assisted selection
Dinesh Kumar Srivastava, Pankaj Kumar
Ajay Kumar Thakur
Dr. Dinesh Kumar Srivastava has retired as Director Extension Education, prior to this he worked as Professor and Head in the Department of Biotechnology, Dr. Y. S. Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, India. He did his post-doctoral studies in the field of Plant Molecular Biology at the Institute of Molecular Genetics, Moscow, USSR and at Washington University, St. Louis, USA. He has 32 years of research and teaching experience in the field of Plant Biotechnology and Molecular Biology. He has guided several M.Sc. and Ph.D. students and published 110 research/ review papers in the journals of national and international repute. He has participated in many national and international conferences. His main area of research includes Plant tissue culture, Genetic transformation and Molecular characterization of plants. He is life member of various National and International Academic bodies/societies. He has receivedmany awards for his scientific contributions.
Dr. Ajay Kumar Thakur is presently working as Senior Scientist (Biotechnlogy) at ICAR-Directorate of Rapeseed-Mustard Research, Bharatpur, Rajasthan. He has published 46 research/review papers in various journals of International and National repute, authored/edited three books, contributed 8 book chapters and 22 popular articles. Dr. Thakur is associated with Brassica juncea improvement programme using biotechnological interventions from last thirteen years. He is presently working on germplasm characterization and association mapping of various agronomically important traits in this oilseed crop. He has been granted one Indian patent and associated in the development of two high yielding Indian mustard varieties and one gobhi sarson variety, and three disease resistant Indian mustard genetic stocks. Dr. Thakur has received many awards from various societies and scientific organizations forhis scientific contribution. He is also an esteemed Member of Plant Tissue Culture Association of India and National Academy of Sciences-India.
Dr. Pankaj Kumar is presently working as Assistant Professor (Biotechnology), Department of Biotechnology, Dr. Yashwant Singh Parmar University of Horticulture and Forestry, Solan, Himachal Pradesh, India. He did his post-doctoral studies at the National Institute of Plant Genome Research, New Delhi and at Council of Scientific & Industrial Research - Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, India. Dr. Kumar research specialization is plant molecular biology, functional genomics, plant genetic engineering, and medicinal plant biotechnology. He has published 35 research/review papers in various journals of International and National repute, contributed 13 book chapters and 5 popular articles. Dr. Kumar has received many awards from various societies and scientific organizations for his scientific contribution.
Book Title : Agricultural Biotechnology: Latest Research and Trends
Editors : Dinesh Kumar Srivastava, Ajay Kumar Thakur, Pankaj Kumar
DOI : https://doi.org/10.1007/978-981-16-2339-4
Publisher : Springer Singapore
eBook Packages : Biomedical and Life Sciences , Biomedical and Life Sciences (R0)
Copyright Information : The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2021
Hardcover ISBN : 978-981-16-2338-7 Published: 09 January 2022
Softcover ISBN : 978-981-16-2341-7 Published: 10 January 2023
eBook ISBN : 978-981-16-2339-4 Published: 08 January 2022
Edition Number : 1
Number of Pages : XV, 726
Number of Illustrations : 1 b/w illustrations
Topics : Agriculture , Biotechnology , Plant Breeding/Biotechnology , Plant Physiology , Plant Biochemistry
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